2/20/2019 Viral Pollution in the Environment and in Shellfish: Human Adenovirus Detection by PCR as an Index of Human Viruses | Applied

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ENVIRONMENTAL AND PUBLIC HEALTH MICROBIOLOGY

Viral Pollution in the Environment and in Shellfish:

Human Adenovirus Detection by PCR as an Index
of Human Viruses
Sonia Pina, Montserrat Puig, Francisco Lucena, Joan Jofre, Rosina Girones

DOI:

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ABSTRACT

A study of the presence of human viruses (adenoviruses, enteroviruses, and hepatitis A viruses [HAVs]) in
environmental and shellfish samples was carried out by applying DNA and cDNA amplification techniques
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by PCR. The detection of human adenoviruses by PCR was also examined as a potential molecular test to
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monitor viral pollution. The samples studied were urban and slaughterhouse sewage, river water, seawater,
and shellfish. Enteroviruses were quantified by PFU in Buffalo green monkey kidney cells and fecal
coliforms and phages of Bacteroides fragilis HSP40 were also evaluated in some of the samples. The
amplification of viral DNA and cDNA has shown a high prevalence of human viruses that would not be
detected by the use of classical techniques, such as the quantification of PFU in cell lines. The results of the
analysis of slaughterhouse sewage samples together with the test of farm animal feces indicate that the
adenoviruses and the HAVs detected in the environment are mostly of human origin. A significative
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correlation between the detection of human viruses by PCR and the values of bacteriophages of B. fragilis
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HSP40 in urban raw sewage was observed. Human adenoviruses were the viruses most frequently detected
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throughout the year, and all the samples that were positive for enteroviruses or HAVs were also positive for
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2/20/2019 Viral Pollution in the Environment and in Shellfish: Human Adenovirus Detection by PCR as an Index of Human Viruses | Applied and En…

human adenoviruses. The results suggest that the detection of adenoviruses by PCR could be used as an
index of the presence of human viruses in the environment where a molecular index is acceptable.

Copyright © 1998 American Society for Microbiology

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