Adventist Medical Center College

Department of Pharmacy
CLINICAL TOXICOLOGY
Experiment number 3
LABORATOY
GASTROINTESTINAL MOTILITY TEST WITH ANTI-ENTERPOOLING TEST (with slight
modification)

Reagents/ Drugs/ Chemicals needed:

Activated Charcoal, Loperamide, Plain NSS

Materials/ Apparatuses/ Glasswares:

50 mL Beakers, Spatula, Stirring rod, 10mL graduated cylinder, oral gavage for mice, rulers,
scissors/scalpel/knife/cutter, needles. Analytical balance

Test animals needed:

2 white albino mice ((Mus musculus (Muridae))

Procedures:
The method that will be used in the experiment is the gastrointestinal method described by
Qnais et. al., and Meite et. al.,(2009) as cited in Tafesse and Mekonnen (2012) with slight
modification suited for the laboratory experimentation.

1) The test animals that will be used will be fasted for 18 hours prior to experimentation but
they will be given water freely.
2) The test animals will be labelled accordingly, one as the positive control and the other as
the negative control.
3) Prior to the experimentation proper, reagents, chemicals and drugs to be administered will
be prepared first. A charcoal food will be mixed first with a ratio of 1:10 (1 gram of activated
charcoal per 10 mL of PNSS).
4) The test animals will be given the positive control (Loperamide 5mg/Kg) and PNSS ) 0.5mL
through oral gavage.
5) After 30 minutes prior to administration of the control groups, 1mL of charcoal food will be
given per experimental animal through oral gavage.
6) Thirty minutes after the administration of charcoal food, the test animals will be sacrificed
and will be laparatomized.
7) The test animals intestines will be removed and the distance covered by the activated
charcoal from the pylorus to the caecum will be measured. The percentage inhibition will be
calculated as follows (Oben et. al, 2006 as cited in Tafesse and Mekonnen, 2012)

TLI − DCC
% 𝐼𝑛ℎ𝑖𝑏𝑖𝑡𝑖𝑜𝑛 = 𝑥 100
TLI

Where: TLI: Total Length of the intestines and DCC: Distance Covered by the Charcoal

8) The intestines will be weighed and the intestinal content will be collected by milking it into
the graduated cylinder and the volume will be measured. The intestines will then be
reweighed and the difference between a full intestine and an empty intestine will be
calculated and recorded as the weight of the intestinal content.

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 Adventist Medical Center College
Department of Pharmacy
CLINICAL TOXICOLOGY
Experiment number 3
LABORATOY
GASTROINTESTINAL MOTILITY TEST WITH ANTI-ENTERPOOLING TEST (with slight
modification)

Name: ________________________________________ Year and Section: __________________

Date: ______________ Group number: _________ Member number: _____________

RESULTS:
Test Test Parameters Mice 1 (Positive Mice 2 ( Negative
control) Control)
Gastrointestinal 1) Total length of intestines
Motility test
2) Distance covered by the

charcoal

3) % Inhibition

Anti- 1) Weight of intestines before

enterpooling
milking.
test
2) Weight of intestines after

milking.

3) Volume of intestinal content

4) Weight of intestinal content

QUESTIONS:

1) What is the MOA of Loperamide?

2) What type of Antimotility agent is loperamide?

3) What type of antidote is castor oil?

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4) What are the different types of laxatives? Give it’s MOA and examples.

5) What are the different types of antidotes? Give at least 3 and explain its mode of detoxification.

6) What is the use of antimotility test?

7) What is the use of anti-enterpooling test?