Centrifuge

To Separate Platelets and Red Blood Cells

Team 3
Scott Kopp, Andrew Czyzowski, Jason Wang

Project For: James Paolino

 Presentation Overview
 Background on Centrifuge

 Our Centrifuge Design and CAD

 Breakdown of Pieces and Materials

 Breakdown of Electrical Components

 Projected Budget

 Questions
 Centrifuge
 Centrifuges are used to separate heavier particles
from lighter particles in a suspension

 Tubes are placed in the centrifuge at an angle

 Uses centripetal acceleration to force heavy

particles to the bottom of a tube

 Wide variety of uses: separate blood components,

oil, and fat from milk
Typical Centrifuge
 Our Design Key Points
Must be able to:
 Separate blood platelets and red blood cells
from a blood sample

 Have variable speeds

 Have temperature control of samples

 Be able to display temperature and RPM

 Separating Procedure
 To separate the red blood cells from a
blood sample, the tube is placed in the
centrifuge with a counter weight in the
chamber across, and the plasma is
decanted after centrifuging

 To separate the blood platelets from the

blood sample, the decanted plasma is
centrifuged again, and the blood
platelets will settle to the bottom
Centrifuge CAD - Front
Centrifuge CAD - Back
Centrifuge CAD – Test Tube
Holder and Thermal Sensor
Centrifuge CAD – Motor and
Gears
Centrifuge Dimensions Plot
Centrifuge CAD - Rotor and Test
Tube Holder
Centrifuge CAD - Lid
Centrifuge CAD - Motor
Centrifuge CAD - Test Tube
Holder
Centrifuge CAD – Heating Coil
Centrifuge Design and Materials
Centrifuge Design and Materials
 Housing will be constructed from powder
coated aluminum for corrosion
resistance

 Rotor and bearing made of steel for

strength

 Rotatable tube holder made of plastic

 Motor
MS22 24-Volt Motor from Dynetic Systems

High Speed Capability, High Torque

Rated up to 8900 RPM

Diameter: 2.25’’

Length: 3.2’’
 Electronic Components
 PCB Board

 LCD Display

 Variable Speed Controls

 Temperature Sensor

 Heat Coil

 Power Supply
PCB Layout
PCB Schematic
 PCB Setup
Inputs to the PCB
 There are 4 inputs for variable speeds of 500,
1000, 1500 and 2000 RPM
 There is an input for the temperature sensor

Output from PCB

 There is an output to the motor based on the
selected speed
 There is an output to the heat coil based on the
selected temperature
 There is an output to the LCD to display that the
centrifuge is running and at what speed and
temperature
 C-code Key Points - Speed
Adjustment
void rpm500()
{
if(SPEED_1)
{
int t
changeRPM(500); //sets Centrifuge Speed to 500 RPMs
clear display(); //clear previous display
DisplayC (0x80, "500 RPM"); //displays "500 RPM" in top left corner
DisplayC (0xC0, "RUNNING"), //displays "RUNNING" in bottom left corner
for(i=120000,i>0,i--) // runs the centrifuge for 20 minutes
{
LoopTime();
t=temperature();
if(t>38)
changeHeat(36);
if(t<36)
changeHeat(38);
}
clear_display(); //clears the display once complete

DisplayC(0x80, "DONE"); //tells the user the cycle is complete

return;
}
}
 Separating Process
Process to separate:
 The red blood cells from a blood sample
 Place sample tube in centrifuge, with counter
weight
 Centrifuge at low temperature, 20 minutes,
1000RPM should be sufficient
 The blood platelets from the blood sample
 Place decanted plasma from previous
centrifugation into sample tube in centrifuge, with
counter weight
 Centrifuge at low temperature, 20 minutes,
1500RPM should be sufficient
 Projected Budget
 Metal for construction: $200
 2 PCB boards $83.62
 MS22 DC Motor $50
 Heating Coil $40
 LCD Display $21.50
 Electrical Components $20
 Test Tube Holder $25

 Total $440.12
 PCB Cost Breakdown
 PCB board from ExpressPCB
 PCB Manufacture and Delivery
Setup fee per order: $55.00
Setup fee for 2 boards: $2.00
Area Cost: $16.77
Shipping: $9.85
Total cost of order: $83.62
Questions?