Fluorescent Protein

Bacteria Expression vector, Driven

by T7 promoter, with a 6xHis tag at the N-terminus.

Box 1 | Product List

Fluorescent Protein Vectors

I MPORTANT NOTICE TO PURCHASER:
These products may be subjects of pending
U.S. and foreign patents. Allele Biotech grants
Vector Name Cat. Number Page academic/government research institutions a
pNCS-mWasabi ABP-FP-WPNCS10 2
worldwide, non-exclusive, royalty-free, limited
license to use this product for non-commercial
pNCS-mTFP1 ABP-FP-TPNCS10 3
life science research use only. Such license
pNCS-LanRFP ABP-FP-RPNCS10 4
specifically prohibits the right to sell or otherwise
pNCS-LanYFP ABP-FP-YPNCS10 5 transfer this product or its derivatives to a third
party.

Industry For-Profit Institutions: Institutions that

wish to use this product are required to obtain a
sub-licensing agreement from Allele Biotech. For
more information please visit Fluorescent Protein
Sublicense, or contact us via phone or email.

F or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer
this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted
use without the express written authorization of Allele Biotech is strictly prohibited

Allele Biotech-Introducing Cost Effectiveness to Research

Alleleustrious pNCS-mWasabi
Recommended Use
(E.Coli bacteria expression)
mWasabi has been optimized for use with standard GFP/FITC filter
sets.
Catalog Number: ABP-FP-WPNCS10 Features
• About 2-fold brighter than EGFP

Introduction • Similar photostability to EGFP

ele • Uses standard filter sets
All ustrious pNCS-mWasabi is a bacterial expression vector that
ele • Can be co-imaged with blue and red FPs or dyes
constitutively expresses 6xHis-tagged mWasabi in E. coli. All ustrious
• Mammalian expression vector ready to transfect your favorite cells
mWasabi is a monomeric green fluorescent protein that can be easily
• Low sensitivity to acidic pH (fluorescence pKa=4.3)
detected using standard GFP filter sets. mWasabi may be used as a direct
• True monomer that will not aggregate or cause nonspecific
replacement for EGFP or other GFPs for superior performance, and may be
interactions
co-imaged with blue and red fluorescent labels without substantial
bleed-through.
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Source
Storage
Engineered variant of mTFP1, originally derived from Clavularia sp. coral. Store at -20°C or at -80°C for long-term preservation.

T7 Promoter………………..........64-151
6X HIS………………………….....152-250 T7
6X HIS
mWasabi.....................................251-961 pUC Ori
T7 Terminator.............................1024-1042 Wasabi

bla Promoter...............................1679-1783 pNCS-

mWasabi
Ampicillin Resistance Gene......1768-2628 T7
3623bp Terminator
pUC Origin..................................2777-3419
F1
Upstream Sequencing Primer: Origin
AMPR
Universal CMV Promoter Primer
BLA
Downstream Sequencing Primer:
SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature
Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of
Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA,
Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09
Alleleustrious pNCS-mTFP1
(E.Coli expression)
Catalog Number: ABP-FP-TPNCS10
Introduction
ele
All ustrious pNCS-mTFP1 is a bacterial expression vector that
constitutively expresses 6xHis-tagged mTFP1 in E. coli. The properties of
ele
All ustrious mTFP1 are superior to those of the commonly used Aequorea
victoria variant ECFP and which can be easily detected in mammalian cells
using standard CFP filter sets. In addition, the fluorescent signal from
mTFP1 is significantly brighter than that from EGFP using standard GFP
filter sets. mTFP1 also makes an excellent fluorescence resonance energy
transfer (FRET) donor to yellow or orange fluorescent proteins.
Source
Engineered variant of Clavularia sp. fluorescent protein cFP484.
Recommended Use
To further optimize detection, the use of filter sets specific to the
mTFP1 excitation and emission spectra is recommended.
Features
• More than 3-fold brighter than ECFP
• More than twice as photostable as ECFP
• Single emission peak
• Single-exponential fluorescence lifetime (τ = 3.2 ns)
• Can be co-imaged with red and yellow FPs
• Low sensitivity to acidic pH (fluorescence pKa=4.3)
• Mammalian expression vector ready to transfect your favorite cells
• True monomer that will not aggregate or cause non-specific
interactions
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Storage
Store at -80°C for long-term preservation.

T7 Promoter………………..........64-151
6X HIS………………………….....152-250 T7
6X HIS
mTFP1….....................................251-961 pUC Ori
T7 Terminator.............................1024-1042 mTFP1

bla Promoter...............................1679-1783 pNCS-mTFP1

Ampicillin Resistance Gene......1768-2628 3623bp T7

Terminator
pUC Origin..................................2777-3419
F1
Upstream Sequencing Primer: Origin
AMPR
Universal CMV Promoter Primer
BLA
Downstream Sequencing Primer:
SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature
Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of
Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA,
Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09

pNCS-LanRFP
(E.Coli bacteria expression)
Catalog Number: ABP-FP-RPNCS10
Introduction
Alleleustrious pNCS-LanRFP is a bacterial expression vector that
constitutively expresses 6xHis-tagged LanRFP in E. coli. The lancelet
fluorescent proteins are a newly discovered, promising group of
fluorescent proteins introduced to the market exclusively from Allele
Biotech. These proteins were discovered in Lancelets (also called
amphioxus), a species of small fish that are usually found buried in sand
in shallow parts of temperate or tropical seas. Lancelets are believed to
be a very primitive chordate species. Compared to jellyfish and coral, the
most common source of virtually all the currently used fluorescent
proteins, Lancelets are closer to mammalians and their proteins may find
great application in human cells and other commonly used animal models.
When expressed, this protein emits a red fluorescence.
Recommended Use
LanRFP has been optimized for use with standard GFP/FITC filter
sets.
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Storage
Store at -20°C or at -80°C for long-term preservation.

pNCS-LanYFP
(E.Coli bacteria expression)
Catalog Number: ABP-FP-YPNCS10
Introduction
Alleleustrious pNCS-LanYFP is a bacterial expression vector that
constitutively expresses 6xHis-tagged LanYFP in E. coli. The lancelet
fluorescent proteins are a newly discovered, promising group of
fluorescent proteins introduced to the market exclusively from Allele
Biotech. These proteins were discovered in Lancelets (also called
amphioxus), a species of small fish that are usually found buried in sand
in shallow parts of temperate or tropical seas. Lancelets are believed to
be a very primitive chordate species. Compared to jellyfish and coral, the
most common source of virtually all the currently used fluorescent
proteins, Lancelets are closer to mammalians and their proteins may find
great application in human cells and other commonly used animal models.
When expressed, this protein emits a yellow fluorescence.
Recommended Use
LanYFP has been optimized for use with standard GFP/FITC filter
sets.
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL of
nuclease-free water for a final concentration of 1 µg/µL.
Storage
Store at -20°C or at -80°C for long-term preservation.