Product Data

AcroPrep™ Membrane-bottom
Filter Plates

Multi-well, Membrane-bottom Plates for

High Throughput Sample Preparation
and Detection Procedures
• Broad selection – available in 96- or 384-well
formats with a variety of membranes, plate color
choices, well volumes, and outlet tip lengths for
use in a multitude of sample preparation and
detection processes.
• Automation friendly – designed in accordance
with the ANSI/Society for Biomolecular Screening
(SBS) standards. Rigid construction enables the
plate to be easily maneuvered by robotic
equipment and assures that plates do not flex
and jam detectors or other instrumentation.
• Low binding, chemically resistant –
• No crosstalk – specially engineered fluid
polypropylene housing assembly is compatible
deflectors and outlet tips on the bottom of
with a broad range of aqueous and organic
the plate were designed to reduce the potential
liquids, and is noted for its low biomolecule
for crosstalk. Elimination of crosstalk is assured
binding so that non-specific adsorption to the
through individually sealing a membrane in each
plate is minimized.
well using proprietary sealing technology.
• Serialized barcodes – serialized barcode labels
allow for the use of automated tracking systems.

Applications

• Sample prep/gross fractionation • Fluorescence detection

• DNA purification, concentration, and fractionation
• Receptor:ligand binding assays
• Prefiltration
• Clarification of cell lysates and tissue homogenates
• Preparation of proteinaceous solutions
• Drug binding studies
• Low protein or nucleic acid binding procedures
• Chemiluminescence detection
• Radioactive (isotopic) detection
• Combinatorial chemistry library screening
• Cleavage assays
• Bead-based assays
• Protein purification, concentration, and fractionation
• Capture from liquid chromatography
• Antibody purification
• Contaminant removal such as DNA, RNA, or other
charged molecules
Plate Selection

Low Biomolecule Binding

Solvent Resistance Required

Hydrophobic Membrane
(Solvent Based Solutions)

PTFE Membrane

Applications
• Molecular Synthesis Reactions
• Drug Synthesis Reactions
• Resin Cleavage Reactions
• Combi-chem Library Screening
• Sample Prep Involving Solvents

96 Well

350 µL 1 mL

0.2 µm 0.45 µm 0.2 µm 0.45 µm

Natural Housing Natural Housing Natural Housing Natural Housing

PN 5037 PN 5038 PN 5055 PN 5056

Low Biomolecule Binding

Solvent Resistance Not Required

Integrated Prefilter

Polypropylene Prefilter Glass Fiber Prefilter

Applications Applications
• Debris Removal • Plasmid Preparation
• Low Salt DNA Purification • Lysate Clearance
• Rapid Plasmid Preparation • Protein Solutions
• Proteinaceous Sample
Purification
• Tissue Homgenates

96 Well 96 Well

350 µL 350 µL 1 mL

Polypropylene/1.2 µm 3.0 µm GF/0.2 µm 3.0 µm GF/0.2 µm

Supor® Membrane Bio-Inert® Membrane Bio-Inert Membrane

PN 5041 PN 5046 PN 5053

 Hydrophilic Membrane
(Aqueous or Solvent Based Solutions)

GHP Membrane
(Hydrophilic Polypropylene)

Applications
• Cleavage Reactions
• In-gel Digestion
• Combi-chem Library Screening
• Drug Binding Studies
• Sample Prep
• Bead/Resin Applications

96 Well 384 Well

350 µL 1 mL 100 µL

0.2 µm 0.45 µm 0.2 µm 0.45 µm 0.45 µm

Natural Housing Natural Housing Natural Housing Natural Housing

PN 5045 Natural Housing White Housing Black Housing PN 5052 PN 5054 Short Tip Long Tip

PN 5030 PN 5043 PN 5044 PN 5071 PN 5070

No Prefilter

Polyester Screen Bio-Inert Membrane Supor® Membrane GHP Membrane

(Hydrophilic Nylon) (Modified PES) (Hydrophilic Polypropylene)

Applications Applications Applications Applications

• Sieve Filtration
• Cytometry Sample Preparation
• Sample Prep • Sample Prep • Cleavage Reactions
• Drug Binding Studies • Drug Binding Studies • In-gel Digestion
• Purification of Oligos from • Purification of Oligos from • Combi-chem Library Screening
Polyacrylamide Gels Polyacrylamide Gels • Drug Binding Studies
• Bead Assays and Applications • Resin-based Purifications • Sample Prep
• Resin-based Purifications • Lysate Clearance • Bead/Resin Applications

96 Well 96 Well 96 Well 96 Well 384 Well See Above

350 µL 350 µL 350 µL 1 mL 100 µL

30-40 µm 0.2 µm 0.45 µm 1.2 µm 1.2 µm 1.2 µm

PN 7267 PN 5042 Short Tip Long Tip

PN 5029 PN 5039, 5039L PN 5065
PN 5085 PN 5084

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Plate Selection

High Biomolecule Binding

Binding Media

Glass Fiber

Applications
• Receptor Ligand Binding Studies
• Cell-based Assays
• Sample Prep
• Gross Fractionation

96 Well 384 Well

350 µL 1 mL 100 µL

1.0 µm 1.0 µm 1.0 µm 1.0 µm 1.0 µm

Natural Housing White Housing Long Tip Natural Housing White Housing
PN 5031, 5031L PN 5032 PN 5051
Short Tip Long Tip Short Tip Long Tip
PN 5073 PN 5072 PN 5073W PN 5072W

Ion Exchange Separations

Membrane Chromatography

Mustang™ Membrane

Applications
• Fractionation of Proteins/Peptides
• Capture from Liquid Chromatography
• Antibody Purification
• Contaminant Removal Such as DNA, RNA,
or Other Charged Molecules

96 Well

350 µL 1 mL

Mustang S Mustang Q Mustang S Mustang Q

(Cation Exchange) (Anion Exchange) (Cation Exchange) (Anion Exchange)

PN 5048 PN 5047 PN 5063 PN 5062

 Molecular Separations

Ultrafiltration

Polyethersulfone Membrane
(OmegaTM Membrane)

Applications
• Concentrate, Desalt, and Purify DNA, RNA,
Oligos, Proteins, and Peptides
• Size Exclusion Applications
• Clean-up of Labeling Reactions
• Determination of Free vs. Bound Drug in Plasma
• Purification of Nucleic Acids and Proteins

96 Well 384 Well

1 mL 350 µL 100 µL

3K 3K 10K 30K 100K 10K 30K 100K

PN 5033 PN 5034 PN 5035 PN 5036

Long Tip Short Tip Long Tip Short Tip Long Tip Short Tip Long Tip

PN 5066 PN 5077 PN 5076 PN 5079 PN 5078 PN 5081 PN 5080

Plate Color Selection Guide

Detection results can be further optimized by selecting the
proper plate color. Use the following recommendations as
a guideline for easy selection.

Natural
(semi-opaque)
– fluorescence, TRF
White
(opaque)
– radioactivity,
chemiluminescence
Black
(opaque)
– lowest background for
fluorescence, TRF

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Specifications

AcroPrep™ 96 Filter Plates, 350 µL Wells

Materials of Construction Dimensions
Housing: Polypropylene (natural, white, or black) Length: 12.78 cm (5.03 in.)
Lid: Polystyrene Width: 8.55 cm (3.37 in.)
Filter Media: Height (without lid): 1.44 cm (0.57 in.)
0.2 and 0.45 µm GHP membrane (hydrophilic polypropylene) Height (with lid): 1.66 cm (0.66 in.)
0.2 and 0.45 µm PTFE membrane (hydrophobic PTFE Well-bottom Area: 0.25 cm2
on polyester support) Maximum Well Volume
3.0 µm Glass Fiber/0.2 µm Bio-Inert® membrane 380 µL
(modified hydrophilic nylon) Recommended Working Volume
0.2 µm Bio-Inert membrane (modified hydrophilic nylon) 300 µL
1.0 µm (nominal) Glass Fiber (borosilicate glass without Recommended Operating Vacuum
binder over polypropylene support) 25.4 cm Hg (10 in. Hg) or greater
3, 10, 30, and 100K Omega™ membrane Filtration by Centrifugation
(modified polyethersulfone) 500-3,000 x g
0.45 and 1.2 µm Supor® membrane (hydrophilic
Typical Sample Hold-up Volume
polyethersulfone)
< 15 µL/well
0.8 µm Mustang™ Q and S membranes
(hydrophilic polyethersulfone)
30-40 µm polyester screen
AcroPrep 96 Well Plates, 1 mL Wells
Materials of Construction Dimensions
Housing: Polypropylene (natural) Length: 12.78 cm (5.03 in.)
Filter Media: Width: 8.55 cm (3.37 in.)
0.2 and 0.45 µm GHP membrane (hydrophilic polypropylene) Height (without lid): 2.87 cm (1.13 in.)
0.2 and 0.45 µm PTFE membrane (hydrophobic PTFE Tip Length: 0.53 cm (0.21 in.)
on polyester support) Well-bottom area: 0.25 cm2
3.0 µm Glass Fiber/0.2 µm Bio-Inert membrane Maximum Well Volume
(modified hydrophilic nylon) 1 mL
1 µm (nominal) Glass Fiber (borosilicate glass without
Recommended Working Volume
binder over polypropylene support)
900 µL
0.8 µm Mustang Q and S membranes
(hydrophilic polyethersulfone) Recommended Operating Vacuum
3K Omega membrane (modified polyethersulfone) 25.4 cm Hg (10 in. Hg) or greater
1.2 µm Supor membrane (hydrophilic polyethersulfone) Filtration by Centrifugation
1,000-3,000 x g
Typical Sample Hold-up Volume
< 27 µL/well
AcroPrep 384 Well Plates, 100 µL Wells
Materials of Construction Long Tip Length: 0.41 cm (0.16 in.)
Housing: Polypropylene (natural) Total Plate Height Including Long Tips: 1.85 cm (0.73 in.)
Lid: Polystyrene Well-bottom Area: 0.05 cm2
Media:
0.45 µm GHP membrane (hydrophilic polypropylene) Maximum Well Volume
100 µL
1 µm (nominal) Glass Fiber (borosilicate glass without binder
over polypropylene support) Recommended Working Volume
1.2 µm Supor (hydrophilic polyethersulfone) 80 µL
10, 30, and 100K Omega ultrafiltration membrane Recommended Operating Vacuum
(modified polyethersulfone) 50.8 cm Hg (20 in. Hg) or greater
Dimensions Filtration by Centrifugation
Length: 12.78 cm (5.03 in.) 500-3,000 x g
Width: 8.55 cm (3.37 in.) Typical Sample Hold-up Volume
Height (with lid): 1.66 cm (0.66 in.) < 4 µL per well
Height (without lid): 1.44 cm (0.57 in.)
Methodology

Filtration vs. Retention

1. Place filter plate on vacuum manifold or hold the plate 2. Add sample and incubate. Apply vacuum.
so that the outlet tips are not touched.

3a. Release vacuum from the manifold. Remove filter plate OR 3b. Release vacuum from the manifold. Remove filter
and retained sample for further processing. plate. Remove collection (receiver) plate and utilize
collected filtrate in downstream applications.

AcroPrep™ Filter Plate Operating Instructions

AcroPrep filter plates are ideally suited for both manual use and high-volume robotics applications.

Manual Vacuum Filtration Procedures Robotic Vacuum Filtration Procedure

1. During use, hold the plate so that the outlets on the 1. AcroPrep filter plates can be stacked with or without
bottom of the plate are not touched. This prevents lids in place.
liquid flow due to wicking.
2. Process the plates in the robotic equipment in the
2. Add the solution to be filtered and incubate. Place same way as a standard solid-bottom plate.
the plate on the vacuum manifold and apply vacuum.
3. Transfer the AcroPrep filter plates to a vacuum
(See product specifications for recommended vacuum.)
manifold station and apply the vacuum. (See product
3. Release vacuum from the manifold. DO NOT RELEASE specifications for recommended vacuum.)
VACUUM BY PULLING THE CORNER OF THE PLATE
AS IT WILL DEGRADE THE MANIFOLD GASKET.
4. Gently tap the plate to remove any hanging droplets.
5. Add wash solution(s) and filter as needed.

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Methodology (continued)

Compatible Robotic Systems

AcroPrep™ filter plates offer a robust platform for sample preparation and detection applications and are compatible
with a variety of currently available robotic systems. Following is a short list of just some of the compatible equipment.

Liquid Handlers Note: Polypropylene is an inert material with low

• Adept Technology, Inc. • Robbins Scientific biomolecule binding. This is important to prevent non-
• Beckman Technologies Corp. specific binding. If a small amount of binding still occurs,
• Cyber Lab • Robocon classical passivation procedures can be used (see
• Hamilton Company • Tecan U.S. Nanosep® Application Guide – PN 32989).
• Matrix Technologies Corp. • Titantele
• Oyster Bay Pump Works, Inc. • Tomtec
• PerkinElmer Inc. • Velocity 11
• Porvair • Waters
• Qiagen, Inc

Vacuum Manifolds
• Pall Life Sciences • Porvair
• 3M • Qiagen, Inc.
• Innovative Microplates • Whatman
• Millipore

Performance

Figure 1
Lower Level of False Positives with AcroPrep 96 Filter Plates Than Competitive Plates

Pall Pall Pall

AcroPrep 96 Competitor M AcroPrep 96 Competitor M AcroPrep 96 Competitor M
3 µg/mL 47 101 4 µg/mL 58 191 5 µg/mL 172 1187
PE-Ab 24 1313 PE-Ab 74 2234 PE-Ab 209 1740
32 678 65 972 86 2146
31 1425 126 1467 120 1084
55 826 54 894 119 4021
30 752 175 806 52 1628
29 1118 38 1064 177 2072
42 1300 41 1663 108 767
10 1728 77 1302 1307 3584
14 692 108 1149 1194 2842
34 743 88 1229 937 319
42 905 215 1957 1170 2657
52 889 168 1012 455 288
29 770 99 2239 696 656
45 403 231 1919 478 1053
14 895 179 1177 17 869

Avg. 33 908 Avg. 112 1330 Avg. 456 1682

SD 13 399 SD 63 556 SD 457 1129
% CV 40.97 43.89 % CV 55.98 41.84 % CV 100.18 67.11

The serological immunoassays were performed with two manufacturer’s 96 well filter plates to compare Competitor M filter plates to Pall AcroPrep 96 filter
plates with 1.2 µm Supor® membrane. The serological immunoassays were performed using a single serum sample and three concentrations of rPhycoerythrin
conjugated antibody. The results from these filter plates were read with one Luminex LX100 Instrument. The results presented are for the sixteen individual
responses, the average, the standard deviation, and the percent coefficient of variation for the single serum sample. The Pall Life Sciences AcroPrep 96 filter
plates exhibit a lower level of false positive results than Competitor M filter plates.
Performance (continued)

Figure 2
AcroPrep™ 96 Filter Plates with Prefilter Speeds Sample Processing Times

12
5042

10 5046

COMP
Flow Rate (mL/sec)

0
100 µL 200 µL
Volume Filtered

The use of a prefilter shortens filtration times and allows greater flow rates
to be maintained at higher loads. Average flow rates for 100 and 200 µL
lysate samples were calculated for the AcroPrep 96 filter plate with Bio-Inert®
membrane (PN 5042), AcroPrep 96 filter plate with glass fiber over Bio-Inert
membrane (PN 5046), and Competitor hydrophilic PVDF plate (COMP). Error
bar indicates standard error (n = 8).

See Pall Corp. Technical Report PN 33308 for complete experimental details.

Figure 3
AcroPrep 96 UF Filter Plates Remove Salts While Maintaining High Sample Recoveries

AcroPrep 96 10K AcroPrep 96 30K

99.1 97.3 94 93 99.2 98.1 94 95

100 100
90 90
80 80
70 70
60 60
50 50
40 40
30 30
20 20
10 10
0 0
% Salt Removed % Protein Recovered % Salt Removed % Protein Recovered

Ovalbumin (45kDa) 0.1 mg/mL BSA (66kDa) 0.1 mg/mL

500 mM NaCl 500 mM NaCl
Ovalbumin (45kDa) 1.0 mg/mL BSA (66kDa) 1.0 mg/mL
200 mM NaCl 200 mM NaCl

In addition to desalting, ultrafiltration is also useful for the concentration of biomolecules. The efficiency of AcroPrep 96 UF filter plates with Omega™
membrane to remove salts and other small molecules is not at the expense of sample recovery. Using the AcroPrep 96 10K filter plate, recovery of ovalbumin
proteins were greater than 90% (n = 3) with salt removal efficiencies at greater than 95%. Using BSA and a 30K MWCO membrane, similiar results were
observed. A representative experiment is shown.

See Pall Corp. Technical Report PN 33309 for complete experimental details.

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Ordering Information
AcroPrep™ 96 Filter Plates, 350 µL well AcroPrep 384 Filter Plates, 100 µL well
Part Number Description Pkg Part Number Description Pkg
5033 3K Omega™ membrane, natural 10/pack 5076 10K Omega membrane, long tips, natural 10/pack
5034 10K Omega membrane, natural 10/pack 5077 10K Omega membrane, short tips, natural 10/pack
5035 30K Omega membrane, natural 10/pack 5078 30K Omega membrane, long tips, natural 10/pack
5036 100K Omega membrane, natural 10/pack 5079 30K Omega membrane, short tips, natural 10/pack
5045 0.2 µm GHP membrane, natural 10/pack 5080 100K Omega membrane, long tips, natural 10/pack
5030 0.45 µm GHP membrane, natural 10/pack 5081 100K Omega membrane, short tips, natural 10/pack
5043 0.45 µm GHP membrane, white 10/pack 5070 0.45 µm GHP membrane, long tips, natural 10/pack
5044 0.45 µm GHP membrane, black 10/pack 5071 0.45 µm GHP membrane, short tips, natural 10/pack
5072 1.0 µm glass fiber media, long tips, natural 10/pack
5037 0.2 µm PTFE membrane, natural †† 10/pack
5072W 1.0 µm glass fiber media, long tips, white 10/pack
5038 0.45 µm PTFE membrane, natural †† 10/pack
5073 1.0 µm glass fiber media, short tips, natural 10/pack
5042 0.2 µm Bio-Inert® membrane, natural 10/pack
5073W 1.0 µm glass fiber media, short tips, white 10/pack
5046 3.0 µm glass fiber media/0.2 µm Bio-Inert 10/pack
membrane, natural 5084 1.2 µm Supor membrane, long tips, natural 10/pack
5031 1.0 µm glass fiber, natural † 10/pack 5085 1.2 µm Supor membrane, short tips, natural 10/pack
5031L 1.0 µm glass fiber, natural †, with lid 10/pack
† Glass Fiber plates have polypropylene support
5032 1.0 µm glass fiber, white † 10/pack
†† PTFE plates have polyester support
5029 0.45 µm Supor® membrane, natural 10/pack
5039 1.2 µm Supor membrane, natural 10/pack Vacuum Manifold
5039L 1.2 µm Supor membrane, natural, with lid 10/pack
Part Number Description Pkg
5041 Prefilter material/1.2 µm Supor membrane, 10/pack
natural 5017 Multi-well plate vacuum manifold 1/pkg

5047 Mustang™ Q ion exchange membrane, natural 10/pack 5014 1 mL receiver plate spacer block 1/pkg

5048 Mustang S ion exchange membrane, natural 10/pack 5015 350 µL receiver plate spacer block 1/pkg

7267 30-40 µm screen, natural 10/pack 5016 Replacement accessory kit 1/pkg
(includes O-ring, gasket and allen wrench)

AcroPrep 96 Filter Plates, 1 mL well Accessories

Part Number Description Pkg Part Number Description Pkg
5066 3K Omega membrane, natural 5/pack 5225 Adapter collar for centrifugation 2/pkg
5052 0.2 µm GHP membrane, natural 5/pack 5230 Cap mat for incubation 5/pkg
5054 0.45 µm GHP membrane, natural 5/pack 5231 Multi-well plate lids 10/pkg
5055 0.2 µm PTFE membrane, natural †† 5/pack
5056 0.45 µm PTFE membrane, natural †† 5/pack
5051 1.0 µm glass fiber media, natural † 5/pack
5053 3.0 µm glass fiber media/0.2 µm 5/pack
Bio-Inert membrane, natural
5065 1.2 µm Supor membrane, natural, 5/pack
with valve mats
5062 Mustang Q ion exchange membrane, natural 5/pack
5063 Mustang S ion exchange membrane, natural 5/pack
Application by Membrane Type
GHP Membrane
• Cleavage Reactions
• Receptor:Ligand Screens
• Bead- and Resin-based Applications
• Plasmid Lysate Clearing
• HPLC Sample Prep and Clarification
• Metabolic Studies
• Applications that Use Harsh Organics
• Combi-chem Library Screens
• General Sample Prep
PTFE Membrane
• Molecular Synthesis
• Drug Synthesis Reactions
• Bead- and resin-based Applications
Bio-Inert® Membrane Omega™ Ultrafiltration Membrane
• Drug Binding Studies • Size Exclusion
• Purification of Oligos from Polyacrylamide Gels • Concentrate, Purify, and Desalt Proteins,
• General Sample Prep Peptides, Oligos, DNA, and RNA
Glass Fiber over Bio-Inert Membrane • DNA Clean-up
• Clarification of Cell Lysates and • Recovery of Proteins, Oligos, and RNA
Tissue Homogenates from Polyacrylamide Gels
• Preparation of Proteinaceous Solutions Supor® Membrane
• Prefiltration • Sample Preparation
• Plasmid Lysate Purification
Glass Fiber Membrane
• Gross Fractionation • Bead- and Resin-based Assays
• Lysate Clarification • Receptor:Ligand Binding Assays
• Cell-based Assays
• DNA Binding

Related Products Available from Pall

• Centrifugal devices for nucleic acid and protein sample • BioTrace™, Biodyne,® and FluoroTrans® Transfer
preparation in the following sample volumes: Membranes offer precise performance and compatibility
Device Sample Volume with nearly every detection system available.
Nanosep® Device up to 0.50 mL
• AcroWell™ 96 Filter Plates are an excellent platform for
Microsep™ Device 0.5 to 3.5 mL
a wide variety of molecular biology detection
Macrosep® Device 3 to 15 mL
applications.
Jumbosep™ Device 15 to 60 mL
• Pall Chromatography Resins for the purification of
• Nanosep MF (microfiltration) Centrifugal Devices
biomolecules and compounds.
are available with Bio-Inert or GHP membranes for low
protein binding and high recoveries in applications such • Enchant™ Protein Purification Kits expedite
as particulate removal prior to sample analysis (GHP proteomic sample prep.
product is HPLC grade) and removal of precipitates.

References

K.J. Valenzano, W. Miller, J. Kravitz, P. Samama, D. Complementary Literature

Fitzpatrick and K. Seeley. 2000. Development of a
Fluorescent Ligand-Binding Assay Using the AcroWell
Filter Plate, J. Biomolecular Screening, 5(6):455-461,
PN 33220.
N. Tang, 2002. Chemiluminescent Detection of Protein
Binding, Genetic Engineering News, 22(8):34.
• Product Data, AcroWell 96 Membrane-bottom Filter
Plates, PN 33306
• Technical Report, Automated Purification of
Combinatorial Libraries Using AcroPrep™ 96 Filter Plate
with GHP Membrane, PN 33245
• Protocol, Desalting/Buffer Exchange for Biomolecules
Using the AcroPrep 96 UF Filter Plate, PN 33309
• Protocol, Lysate Clearance for Prokaryotic DNA Isolation
Using the AcroPrep 96 Filter Plate, PN 33308
• Product Brochure, Accelerate Protein Sample
Preparation and Analysis, PN 33411
• Sales Sheet, AcroPrep and AcroWell Multi-well Filter
Plates PN 33287
• Scientific Technical Report, The AcroWell Filter Plate
Minimizes Crosstalk, online
• Protein Sample Preparation and Analysis Application
Manual, PN 33465

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© 2007, Pall Corporation. Pall, , AcroWell, AcroPrep, Biodyne, Bio-Inert, BioTrace, Emflon, Enchant, FluoroTrans,
Jumbosep, Macrosep, Microsep, Nanosep, Omega, and Supor are trademarks of Pall Corporation. ® indicates a
trademark registered in the USA. is a service mark of Pall Corporation.

8/07, pdf, GN07.1641 PN33296