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Atomic absorption units have four basic parts: interchangeable lamps that emit
light at element-specific wavelengths, a sample aspirator, a flame or furnace apparatus
for volatilizing the sample or hydride generation apparatus and a photon detector. In
order to analyze for any given element, a lamp that produces a wavelength of light that
is absorbed by the element is chosen. Subsequently, a sample solution containing the
element is aspirated into the flame. If ions of the element are present in the flame, they
will absorb light produced by the lamp before it reaches the detector. The amount of
light absorbed depends on the amount of the element presents in the sample.
Absorbance values for unknown samples are compared to calibration curves prepared
by running known standard concentrations.
An atomic absorption spectrophotometer (AAS) is a microprocessor controlled double
beam spectrophotometer equipped with a graphite furnace attachment which is employed for
flameless analyses. Both acetylene and nitrous oxide fuel mixtures can be used for the
analyses of a wide range of elements.
Science Officer
Division of Instruments and MUPA
School Of Chemical Sciences, USM, 11800, Penang.