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  • SIKLAMAT

    Transféré par

    reni
    8 vues7 pages
    The document describes a procedure for quantitative analysis of sodium saccharin using high performance liquid chromatography (HPLC). Key details include: - A standard curve was generated using sodium saccharin standards of 100-1000 ppm to quantify sample concentrations. - The HPLC system used a C18 column with a mobile phase of phosphate buffer (pH 5.5):acetonitrile (75:25) at 1 mL/min flow rate and detected at 196 nm. - Repeatability and reproducibility tests on sample concentrations of 200-1000 ppm showed precision of below 1%. - Recovery tests at 200, 600, 1000 ppm found recovery percentages between 4-112%, indicating acceptable accuracy.

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    The document describes a procedure for quantitative analysis of sodium saccharin using high performance liquid chromatography (HPLC). Key details include: - A standard curve was generated using sodium saccharin standards of 100-1000 ppm to quantify sample concentrations. - The HPLC system used a C18 column with a mobile phase of phosphate buffer (pH 5.5):acetonitrile (75:25) at 1 mL/min flow rate and detected at 196 nm. - Repeatability and reproducibility tests on sample concentrations of 200-1000 ppm showed precision of below 1%. - Recovery tests at 200, 600, 1000 ppm found recovery percentages between 4-112%, indicating acceptable accuracy.

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    © All Rights Reserved
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    8 vues7 pages

    SIKLAMAT

    Transféré par

    reni
    The document describes a procedure for quantitative analysis of sodium saccharin using high performance liquid chromatography (HPLC). Key details include: - A standard curve was generated using sodium saccharin standards of 100-1000 ppm to quantify sample concentrations. - The HPLC system used a C18 column with a mobile phase of phosphate buffer (pH 5.5):acetonitrile (75:25) at 1 mL/min flow rate and detected at 196 nm. - Repeatability and reproducibility tests on sample concentrations of 200-1000 ppm showed precision of below 1%. - Recovery tests at 200, 600, 1000 ppm found recovery percentages between 4-112%, indicating acceptable accuracy.

    Droits d'auteur :

    © All Rights Reserved
    Téléchargez comme XLSX, PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    sur 7

    bobot penimbangan : RS : 10.

    07 mg
    potensi RS
    BB sodium siklamat 100.6 mg
    sampel
    cara kerja : RS 1000 ppm : 10 mg/10 mL
    std induk 1000 ppm: 1000 mg/100 ml Akuabides
    RS
    Konsentrasi (ppm) Area RT (min) Tailing Kprime Plate Count
    1467076 3.759 0.828 6.518 12154
    1476863 3.761 0.829 6.521 12419
    1469570 3.752 0.831 6.505 12488
    1007
    1470574 3.747 0.833 6.494 12293
    1467297 3.765 0.818 6.531 11643
    1468393 3.748 0.835 6.496 12374
    Rerata 1469962.16666667 3.755 0.829 6.511 12228.5
    SD 3634.4185458842 0.007 0.006 0.015 309.0
    %RSD 0.25 0.20 0.72 0.23 2.5

    Bahan Baku
    Ulangan Area kadar (ppm) Rerata SD %RSD
    1 1517254 1038.37
    2 1509565 1033.10
    3 1509809 1033.27
    1031.59 4.155 0.403
    4 1500926 1027.19
    5 1502506 1028.27
    6 1504101 1029.36
    Sampel 3941
    Ulangan Area kadar (ppm) RT (min) Tailing Kprime
    1 180538 123.56 3.759 0.828 6.518
    2 179706 122.99 3.761 0.829 6.521
    3 415085 284.07 3.752 0.831 6.505

    DERET KURVA
    [Konsentrasi] Area Rerata
    100 401678 401678 150736.0167
    200 504430 504430 301472.0333
    400 767890 767890 602944.0667
    600 1034402 1034402 904416.1
    800 1273837 1273837 1205888.133
    1517254
    1509565
    1509809
    1000 1507360.1667
    1500926
    1502506
    1504101

    1600000
    1400000 f(x) = 1247.6529817352x + 270312.153881279
    1200000 R² = 0.9992980708
    1000000
    800000
    600000
    400000
    200000
    0
    0 200 400 600 800 1000 1200

    sistem kromatografi :
    Fase gerak : buffer pospat pH 5.5 : asetonitril (75:25)
    Laju alir : 1 mL/menit
    Panjang gelombang : 196 nm
    Vol. Injeksi : 20 µL
    Kolom : C18 Shim pack250x4.6 mm
    Plate Count
    12154
    12419
    12488
    tgl :26 maret 2019
    bobot penimbangan :

    BB sodium siklamat 200.3


    sampel
    cara kerja : std induk 2000 ppm : 200 mg/100 mL akuabides
    sampel : 1 sachet (0.11g/sachet)/50 ml akuabides, disaring, pipet 5 ml/25 ml akuabides
    DERET KURVA
    [Konsentrasi] Area Rerata
    40 304530 304530
    100 362806 362806 1600000
    1400000 f(x) = 1285.8806069447x + 24532
    200 509950 509950 R² = 0.9980833898
    1200000
    400 763886 763886
    1000000
    600 984897 984897
    800000
    800 1308116 1308116
    600000
    1000 1520722 1520722
    400000
    y=a+bx 200000
    a (intercep) 245320.270599118 0
    b (slope) 1285.8806069447 0 200 400 600 800

    PRESISI REPEAT
    ULANGAN Area konsentrasi (ppm)
    1 8728446 6597.13
    2 8759991 6621.67
    3 8658644 6542.85
    Rerata 6587.22
    %RSD 0.612

    PRESISI REPRODUCIBILITY
    ULANGAN Area konsentrasi (ppm)
    1 8710405 6583.10
    2 8663782 6546.85
    3 8663390 6546.54
    Rerata 6558.83
    %RSD 0.321

    AKURASI
    KONSNTRASI Area spk+spike Area spike konsentrasi (ppm)
    200 8967085 256680.00 8.83
    600 9771040 1107258.00 670.31
    1000 10160815 1497425.00 973.73

    sistem kromatografi :
    Fase gerak : buffer pospat pH 5.5 : asetonitril (75:25)
    Laju alir : 1 mL/menit
    Panjang gelombang : 196 nm
    Vol. Injeksi : 20 µL
    Kolom : C18 Shim pack250x4.6 mm
    mg

    et 5 ml/25 ml akuabides

    f(x) = 1285.8806069447x + 245320.270599118


    R² = 0.9980833898

    0 200 400 600 800 1000 1200

    %recovery
    4.42
    112.42
    98.47
    tgl :28 maret 2019
    bobot penimbangan :

    BB sodium siklamat 200.3


    sampel
    cara kerja : std induk 2000 ppm : 200 mg/100 mL akuabides
    sampel : 1 sachet (0.11g/sachet)/50 ml akuabides, disaring, pipet 5 ml/25 ml akuabides
    DERET KURVA
    [Konsentrasi] Area Rerata
    100 421752 421752
    1800000
    200 560982 560982 1600000
    f(x) = 1357.1985479452x + 288
    400 818638 818638 1400000
    R² = 0.9992080272
    600 1128379 1128379 1200000
    1000000
    800 1368250 1368250 800000
    1000 1640294 1640294 600000
    y=a+bx 400000
    a (intercep) 200000
    288496.583561644
    0
    b (slope) 1357.1985479452 0 200 400 600 800

    PRESISI REPEAT
    ULANGAN Area konsentrasi (ppm)
    1 4684186 3238.80
    2 4668177 3227.00
    3 4627326 3196.90
    Rerata 4659896.33 3220.90
    %RSD 0.629 0.671

    AKURASI
    KONSNTRASI Area spk+spike Area spike konsentrasi (ppm)
    200 4804841 144944.67 -105.77
    600 5021118 361221.67 53.58
    1000 6661904 2002007.67 1262.54

    sistem kromatografi :
    Fase gerak : buffer pospat pH 5.5 : asetonitril (75:25)
    Laju alir : 1 mL/menit
    Panjang gelombang : 196 nm
    Vol. Injeksi : 20 µL
    Kolom : C18 Shim pack250x4.6 mm
    mg

    et 5 ml/25 ml akuabides

    f(x) = 1357.1985479452x + 288496.583561644


    R² = 0.9992080272

    0 200 400 600 800 1000 1200

    %recovery
    -52.89
    32.01
    122.05

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