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a) Glucose-1-P
b) Glucose-6-P
c) UDP- Glucose
d) UTP
e) ATP.
2) A 30- year-old male presents with severe muscle cramps. He is found to have
us le gl oge phospho lase defi ie M A dle s disease, Gl oge sto age
type 5).Glycogen phosphorylase degrades glycogen to produce-
a) Glucose
b) Glucose-1-P
c) Glucose-6-P
d) UDP Glucose
e) Glycogen Primer
a) Glycogen synthase
b) Glycogen phosphorylase
c) Glucokinase
d) Hexokinase
4) A 30-year-old presents with intractable vomiting and inability to eat or drink for
the past 3 days. His blood glucose level is normal. Which of the following is most
important for maintenance of blood glucose ?
a) Liver
b) Heart
c) Skeletal muscle
d) Lysosome
e) Spleen.
5) Shortly after birth, an infant presents with tremors, irritability and seizures. His
blood glucose is profoundly low, and he is fed through a nasogastric tube. he is
diagnosed with glycogen storage disease type I, a disease that causes excessive
build up of liver glycogen, resulting in abnormally low blood glucose level. In
glycogen, the linkage at branch points is:
a α -1,4
α-2,3
α-1,6
e β-2,3.
a) Phosphorylase b
b) Hexokinase
c) Glucose-6-phosphatase
d) Glycogen synthase
e) Lactate dehydrogenase
a) Phosphoglucomutase
α-1,6 glucosidase
d) Glycogen primer
e) Uridine diphosphate
10) In glycogen, glucose residues form a straight chain via which of the followings-
a α -1,4 linkages
α -1,6 linkages
b) Requires Phosphoglucomutase
d) Inhibited by c AMP
13) During the breakdown of glycogen, free glucose is formed from which of the
following ?
e) Hydrolysis of glucose-1-P
14) Which of the following statements explains the synthesis of glycogen directly
from D- Glucose ?
c) It occurs in erythrocytes.
d) It requires UDP-Glucose
e) Only Glucose-I-P
17) A newborn is found to have fasting hypoglycemia. The nursery staff begins
overnight feeds by nasogastric tube because they find that the child consistently
has low blood glucose. A liver biopsy and molecular studies demonstrate an
absence of Glycogen synthetase. The normal function of this enzyme is to do
which of the followings ?
18) A 3-year-old child presents to the pediatrician for failure to thrive. A workup
including an ultrasound of his liver shows cirrhosis. A biopsy of liver demonstrates
a deficiency of an enzyme involved in glycogen synthesis. Which of the following
is the most likely glycogen storage disease ?
b)Type II – Po pe s disease
a) Glycogenin deficiency
c) A glucose-6-phosphatase deficiency
a) Phosphorylase kinase
b) Calmodulin
c) Phosphorylase
d) Protein kinase A
e) Glycogen synthase
a)Epinephrine
b) Glucagon
c) Insulin
d) Phosphorylation
22) An infant was brought in to the emergency room after her parents witnessed
he ha i g seizu es. The hild s lood glu ose as 8 ol/L. Afte a tho ough
workup, a GSD was suspected, and a muscle biopsy was significant for the
accumulation of dextrin, a form of glycogen with branching limited to only a few
glucose molecules. Which of the following GSD is most likely the cause of
hypoglycemia and subsequent seizures ?
b)Type II – Po pe s disease
c) conversion to 1,6-bisphosphogluconate.
a) 6-phosphogluconate dehydrogenase
b) Aldolase
c) Glycogen phosphorylase
d) Phosphofructokinase-1
e) Pyruvate kinase
4) In a tissue that metabolizes glucose via the pentose phosphate pathway, C-1 of
glucose would be expected to end up principally in:
a) Carbon dioxide
b) Glycogen
c) Phosphoglycerate
d) Pyruvate
e) Ribulose 5-phosphate
c) supply energy
d) supply NADH
d) They are not required for the production of NADPH in the mature red blood
cells
8) In the pentose phosphate pathway, the major products are which of the
followings ?
9) Which of the following statements about the oxidative section of the pentose
phosphate pathway is correct?
10) Which of the following statements about the nonoxidative section of the
pentose phosphate pathway is correct?
11) Which of the following statements about the use of the NADPH generated
from the pentose phosphate pathway is not correct?
a) NADPH generated from the pentose phosphate pathway is used for steroid
synthesis.
b) NADPH generated from the pentose phosphate pathway is used for the
regeneration of glutathione to its reduced state.
c) NADPH generated from the pentose phosphate pathway is used for the
synthesis of fatty acids
e) NADPH generated from the pentose phosphate pathway is used for the
Macrophageal functions.
a) Fructokinase
b) Aldolase B
c) Glucose-6-P dehydrogenase
d) Galactokinase
e) Galactosyl Transferase.
13) A 50 -year-old alcoholic male presents with pain, numbness, tingling and
weakness in his feet. He is diagnosed with thiamine deficiency. Thiamine and ATP
condense together to form thiamine pyrophosphate, a cofactor important for
the e z es that atal ze o idati e de a o latio of α-keto acids) in the TCA
cycle and also at one of the steps in pentose phosphate pathway, which out of the
following enzymes requires TPP as a coenzyme ?
a) Glucose-6-P dehydrogenase
b) Transaldolase
c) Transketolase
d) 6-P-Gluconate dehydrogenase
e) Gluconolactone hydrolase.
a) Glucose-6-P dehydrogenase
b) Transaldolase
c) Transketolase
d) 6-P-Gluconate dehydrogenase
e) Gluconolactone hydrolase.
15) Xylulose 5-phosphate can be formed from Ribulose 5-phosphate by the action
of-
a) Glucose-6-P dehydrogenase
b) Transaldolase
c) Transketolase
d) Phosphopentose epimerase
e)Phosphopentose isomerase.
c) Pentoses the major product of this pathway are not required by muscle tissue
d) Muscle tissue contains very small amount of enzymes of non oxidative phase
17) In rapidly dividing cancer cells the need for large amount of ribose is fulfilled
by-
16)-a 17)-e
a) Pyruvate
b) Oxaloacetate
c) Malate
d) Acetyl co A
e) α- Keto glutarate
a) 3-phosphoglycerate kinase.
b) Glucose 6-phosphatase
c) Hexokinase
d) Phosphofructokinase-1
e) Pyruvate kinase
d) It is one of the ways that mammals maintain normal blood glucose levels
between meals.
6) All of the following enzymes involved in the flow of carbon from glucose to
lactate (glycolysis) are
a) 3-phosphoglycerate kinase.
b) Aldolase
c) Enolase
d) Phosphofructokinase-1
e) Phosphohexoseisomerase
7) In humans, gluconeogenesis:
b) Glutamate
c) Palmitate
d) Pyruvate
e) α-Keto glutarate.
9) A 6-year-old boy begins playing soccer in a community league. After his first
game of the year, he is brought to see his pediatrician because of his severe muscle
cramps and blood in urine. He is subsequently found to be having deficiency of
lactate dehydrogenase. This enzyme is important in which of the following
conversions ?
a) Pyruvate to Acetyl co A
b) Pyruvate to Oxaloacetate
c) Pyruvate to Alanine
e) Pyruvate to lactate
b) It is induced by Insulin
a) Spleen
c) Skeletal muscle
d) Liver
e) Brain
c) Erythrocytes
a) Pyruvate carboxylase
c) Glucose-6-phosphatase
d) Fr 1,6 bisphosphatase
e) Phosphoglycerate kinase.
14) In an individual at rest, who has fasted for 12 hours, which of the following
occurs ?
a) Pyruvate
b) Oxaloacetate
c) Malate
d) Glucose-6-phosphate
e) Phosphoenolpyruvate.
a) Biotin is required
a) Glucokinase
b) Phosphofructokinase-1
c) Aldolase B
d) Phosphoglycerate kinase
e) Pyruvate kinase.
b) Requires hexokinase
a) Promote gluconeogenesis
b) Promote glycogenolysis
a) Glycogen
b) Glycerol
d) Galactose
e) Glucose-1- P.
Answers- 1)-b , 2)-d, 3)-a, 4)-a, 5)-b, 6)-d, 7)-a, 8)-c, 9)-e, 10)-c, 11)-d,
12)-e, 13)-a, 14)-e, 15)-d, 16)-a ,17)-d, 18)-d,
19)-e, 20)-b.
TCA CYCLE
a) Citrate synthase
b) Isocitrate dehydrogenase
d) Succinate dehydrogenase
e) Malate dehydrogenase
a) Succinate
b) Malate
c) α-Keto glutarate
d) Isocitrate
e) Pyruvate.
a) Thiamine
b) Lipoic acid
c) Pantothenic acid
d) Niacin
e) Ascorbic acid.
a) Thiamine
b) Lipoic acid
c) Pantothenic acid
d) Niacin
e) Riboflavin.
b) Acetoacetic acid
c) Fumaric acid
d) Lactic acid
e) Hydrochloric acid.
a) Succinyl co A
b) Acetyl co A
c) Succinate
d) Malate
e) Pyruvate
a) Succinyl co A
b) Acetyl co A
c) Succinate
d) Malate
e) Pyruvate.
a) Succinate
c) α-Keto glutarate
d) Isocitrate
e) Pyruvate.
a) Succinate Thiokinase
b) Succinate dehydrogenase
c) Citrate synthase
d) Isocitrate dehydrogenase
e) Malate dehydrogenase
a) Citrate synthase
b) Aconitase
c) Succinate dehydrogenase
d) Isocitrate dehydrogenase
e) Malate dehydrogenase.
a) Citrate synthase
b) Aconitase
c) Succinate dehydrogenase
e) Malate dehydrogenase.
12) A 56-year- old chronic alcoholic has been brought in a semiconscious state to
the medical emergency. Blood biochemistry reveals hypoglycemia with blood
glucose level of 45 mg/dl. Which of the following intermediates of TCA cycle can
be directly converted to phosphoenolpyruvate to trigger the pathway of
gluconeogenesis ?
a) Succinate
b) Malate
c) α-Keto glutarate
d) Oxaloacetate
e) Pyruvate.
a) 32
b)2
c) 34
d) 36
d) 38
14) Which of the following allosteric modulators is not effective in influencing the
rate of TCA cycle ?
a) NADH
b) FADH2
c) Ca++
e) ATP
15) How many molecules of CO2 are produced per mole of Acetyl co A in TCA
Cycle ?
a) 2
b) 1
c) 3
d) 0
e) 4
16) The cytosolic isoform of which of the following TCA cycle enzymes requires
NADP+ as a coenzyme, that can further be utilized in the reduced state for
reductive biosynthesis?
a) Citrate synthase
b) Aconitase
c) Succinate dehydrogenase
d) Isocitrate dehydrogenase
e) Malate dehydrogenase.
a) Succinate
b) Malate
c) α-Keto glutarate
d) Oxaloacetate
18)- Which of the following enzymes is Thiamine dependent and essential for
glucose oxidation in the brain?
b) Acetyl co A carboxylase
c) Transaldolase
d) Succinyl-co A Thiokinase
e) Succinate dehydrogenase
a) Malate dehydrogenase
b) Pyruvate carboxylase
c) Pyruvate kinase
d) Citrate synthase
e) Succinyl-co A Thiokinase
20) -Which of the following intermediates of TCA cycle cannot be utilized for
gluconeogenesis ?
a) Succinate
b) Malate
c) α-Keto glutarate
d) Oxaloacetate
e) Acetyl co A.
2). A 45- year- old woman is diagnosed with breast cancer. The oncologist orders a
positron emission tomography(PET) scan of the head to rule out metastasis. This
imaging modality covalently links a radioactive isotope most commonly to
Glucose to appreciate highly active areas in the body such as tumor. Which of the
following traps the tracer in the cell ?
a) Insulin
c) GLUT-4
d) Glucokinase
e) PFK-1
a) Liver
d) Brain tissue
e) Small intestine.
4) A 24-year woman is getting training for her first marathon. Her coach instructed
her to keep a pace that allows her to stay below her anaerobic threshold. Under
such conditions pyruvate does not accumulate as it is converted to-
a) Ethanol
b) Lactic acid
c) Acetyl co A
d) Alanine
e) Oxaloacetate.
a) Fr-2,6 bisphosphate
b) 2,3 bisphosphoglycerate
c) Citrate
d) Acetyl co A
e) Fr-1,6 bisphosphoglycerate
a) Acetyl co A
b) Glucose
c) Lactate
d) ATP
e) Alanine
b) 1
c) 2
d) 3
e) 4
a) Aldolase
b) Glyceraldehyde-3-P dehydrogenase
c) Enolase
d) Phosphoglycerate kinase
e) Phosphoglycerate mutase
10) After a heavy meal, which out of the following allosteric activator would be
most effective in increasing the rate of glycolysis ?
a) ATP
b) Citrate
c) Acetyl co A
d) Fr-2,6 bisphosphate
e) 2,3 bisphosphoglycerate.
11) Compared with the resting state a vigorously contracting muscle shows-
a) Phosphoglucose isomerase
b) Phosphofructokinase
c) Hexokinase
d) Glucokinase
e) Pyruvate kinase.
a) Hexokinase
b) Phosphofructokinase
c) Glyceraldehyde-3-P dehydrogenase
d) Lactate dehydrogenase
e) Enolase
a) Hexokinase
b) Phosphofructokinase
c) Glyceraldehyde-3-P dehydrogenase
d) Lactate dehydrogenase
a) Hexokinase
b) Phosphofructokinase
c) Glyceraldehyde-3-P dehydrogenase
d) Lactate dehydrogenase
e) Pyruvate dehydrogenase
Answers-
a) Hexokinase
b) Glucokinase
c) 3-phosphoglycerate kinase
d) Phosphofructokinase-1
2) Which out of the following statements is not true about aerobic glycolysis ?
a) Is catalyzed by glucokinase
d) Is an irreversible step
a) Oxidative phosphorylation.
c) Oxidative decarboxylation.
d) Phosphorolysis
e) Oxidative deamination
6). The enzyme that produces NADH from a triose phosphate in the glycolytic
pathway-
7). The enzyme that catalyzes first substrate level phosphorylation in glycolysis-
d) Is called phosphofructokinase
8). The enzyme that catalyzes the second substrate level phosphorylation of
glycolysis-
a) Is called phosphoglyceromutase
e) Is stimulated by Glucagon.
9). Which of the following statements about the Lactate dehydrogenase (LDH)
reaction is false
10). The two major factors determining whether a cell oxidizes glucose by aerobic
glycolysis or by anaerobic glycolysis are-
a) 7; 20
b) 2; 38
d)2; 12
e) 7; 25
13). All of the following help to explain some cases of Lactic Acidosis except-
a) High altitude
b) Heart failure
14) . All of the following are part of the Cori Cycle except-
15). The ATP/AMP ratio has a major effect upon the rate of ATP production by
glycolysis. ATP and AMP bind to allosteric sites on-
a) Hexokinase
b) Glucokinase
c) Phosphofructokinase-1
d) Phosphofructokinase-2
e) 3-phosphoglycerate kinase
a) Creatine phosphate
b) ATP
c) ADP
d) AMP
e) Pi
18). In the liver, glucagon causes a decrease in the glycolytic pathway by all of the
following mechanisms except-
20).During fasting state, the insulin to glucagon ratio drops and all of the following
occur except-
b) Glucokinase
c) PFK-1
d) PFK-2
a) Alanine
b) Glutamate
c) Glycerol
d) Pyruvate ( c )
a) Aerobically, oxidative decarboxylation of pyruvate forms acetate that enters the citric acid
cycle.
d) Under anaerobic conditions pyruvate does not form because Glycolysis does not occur.
a) ATP synthesis.
Q.4 – The oxidation of 3 mol of glucose by the pentose phosphate pathway may result in the
production of:
Q.5 – How many ATP molecules can be derived from each molecule of acetyl CoA that enters
the K e s C le?
a) 6
b) 12
c) 18
d) 38 ( b )
Q.6 – All of the following vitamins except one participate in the TCA cycle-
a)Pantothenic acid
b)Lipoic acid
c) Folic acid
d)Riboflavin ( c )
Q.8 - All are correct about pyruvate dehydrogenase complex except one-
d) Pyruvate as well as ADP (a signal of low energy charge) inhibits the complex. ( d )
Q.10 – Which of the following is not an intermediate of the citric acid cycle?
a) Acetoacetate
b) Citrate
c) Oxalosuccinate
d) Succinyl-CoA ( a )
Q.11 - In an anaerobic system that is metabolizing glucose as a substrate, which of the following
compounds would you expect to increase in concentration following the addition of fluoride?
a) 2-phosphoglycerate
b) Glucose
c) Phosphoenolpyruvate
a) ATP
b) Cu2+
c) Heme
d) NAD+ ( d )
a) 3-phosphoglycerate kinase.
b)Glucose 6-phosphatase.
C) Hexokinase.
d)Phosphofructokinase-1. ( a )
Q.14 – Which of the following statements about the pentose phosphate pathway is incorrect?
Q.15 – Which of the following enzymes catalyzes a reaction that involves a decarboxylation
reaction?
a)Pyruvate dehydrogenase
b) Isocitrate dehydrogenase
Q.16 -Anaplerotic reactions are those that result in replenishing intermediates in the TCA cycle.
Which of the following enzymes catalyzes an Anaplerotic reaction?
b) Pyruvate carboxylase
c) Pyruvate kinase
Q.17- Which statement BEST describes the fate of Propionyl-CoA in mammalian systems?
a) Propionyl-CoA is metabolized via are action sequence that involves vitamin B12 and biotin.
c) Propionyl-CoA and acetyl CoA condense to form a 5 carbon precursor of a TCA cycle
intermediate.
Q.18 – It is very important to feed the baby very soon after birth, because during the first few
hours after birth the enzyme Phosphoenolpyruvate carboxykinase is present in very low
amounts, and this fact compromises:
a) Gluconeogenesis
b) Glucose phosphorylation
c) Glycogenesis
d) Glycogenolysis ( a )
a) Fluoroacetate
b) Aerobic conditions
c) Malic acid
d) Fluorouracil ( a )
b) Phosphorylase
d) Glucose-6- phosphatase ( a )
Q.21 – A medical student developed hemolytic anemia after taking the oxidizing Antimalarial
drug primaquine. This severe reaction is most likely due to
b) Concomitant Scurvy
c) Diabetes
Q.22- Which of the following explains why individuals with hyperlipidemia should minimize
their intake of sucrose and high fructose syrup?
a) 2,3bisphosphoglycerate
b) 1,3bisphosphoglycerate
d) Lactate ( a )
c) Pyruvate
Q.24 – Poorly perfused areas exposed to chronic hypoxia have decreased metabolic energy for
tissue maintenance and repair an important reason for this is-
b) Decreased ATP production and increased glucose utilization due to an aerobic mode of
glycolysis
d) Decreased respiratory quotient on changing from carbohydrates to fats as the major fuel
( b )
Q.25 – Asians and Native Americans may flush and feel ill after drinking a small amount of
ethanol in alcoholic beverages. This reaction is due to genetic variation in an enzyme that
metabolizes the liver metabolite of alcohol, which is-
a) Methanol
b) Acetone
c) Acetaldehyde
d) Glycerol ( c )
Q.26 – Which one of the following enzymes catalyzes the phosphorylation of the substrate with
the use of inorganic phosphate-?
a) Hexokinase
b) Phospho fructokinase
c) Glyceraldehyde-3-phosphate dehydrogenase
b) Acetyl co A carboxylase
c) Transaldolase
d) Succinyl-co A Thiokinase ( a )
a) FMN
b) Biotin
c) NAD+
d) TPP ( b )
a) Glucose-6 phosphate
b) Fructose 1, 6 bisphosphate
c) Fructose-6- phosphate
d) 2, 3 bisphosphoglycerate ( d )
a) Hexokinase
b) Aldolase
c) Galactokinase
c) Malic enzyme
d) Glucose-6-phophatase ( d )
Q.7- Which of the following product of Triglyceride breakdown and subsequent beta
oxidation would undergo gluconeogenesis-
a) Propionyl Co A
b) Acetyl CoA
c) Aceto acetate
b) Pyruvate to Lactate
a) Simple diffusion
a) Xylulose-5- phosphate
b) Glucose-6-phosphate
c) Ribulose-5-phosphate
d) Glyceraldehyde-3- phosphate ( a )
a) Hepatic hexokinase
b) Muscle Phosphorylase
d) Muscle Hexokinase ( b )
a) Hepatic Glycogenolysis
b) Gluconeogenesis
c) Muscle Glycogenolysis
a) Glucose
b) Fructose
c) Galactose
Q.14- In the fasting state there is considerable release of———–from the muscles.
a) Glycine
b) Alanine
c) Valine
d) Glutamic acid ( b )
a) Hepatomegaly
b) Splenomegaly
c) Cataract
d) Mental retardation ( b )
a) Lactate<———–>Pyruvate
b) Oxaloacetate—->Phosphoenol pyruvate
c) Glucose-6-phosphate—– >Glucose
d) Phosphoenol pyruvate–>Pyruvate ( b )
a) Adipose tissues
b) Skeletal muscles
c) Brain
a) Succinate dehydrogenase
b) Succinate Thiokinase
c) Succinate lyase
d) Succinate Thioesterase ( b )
b) SH linkages
a) Glucose-6 phosphate
b) Fructose 1, 6 bisphosphate
c) Fructose-6- phosphate
d) Glycerol – 3- phosphate ( d )
b) Transaldolase
c) Transketolase
Q.22- The net production of ATP in glycolysis during anaerobic condition is:
Q.23- Which of the followings does not generate free glucose during the enzymatic
breakdown of glycogen in skeletal muscles?
a) Phosphorylase
b) α-1-6-amyloglucosidase
c) Debranching enzyme
d) glucose-6-phosphatase ( a )
Q.24- Which of the followings promotes glucose and amino acid uptake by muscle?
a) Adrenaline
b) Insulin
c) Glucagon
d) Cortisol ( b )
Q.25- In a tissue that metabolizes glucose via the pentose phosphate pathway, C-1 of glucose
would be expected to end up principally in:
a) Carbon dioxide
b) Glycogen
d) Pyruvate ( a )
a) Alanine
b) Glutamate
c) Glycerol
d) Pyruvate
d) Under anaerobic conditions pyruvate does not form because Glycolysis does
not occur
a) ATP synthesis.
Q.5 – How many ATP molecules can be derived from each molecule of acetyl
CoA that e ters the Kre s’ Cy le?
a) 6
b) 12
c) 18
d) 38
Q.6 – All of the following vitamins except one participate in the TCA cycle-
a) Pantothenic acid
b) Lipoic acid
c) Folic acid
d) Riboflavin
d) Pyruvate as well as ADP (a signal of low energy charge) inhibits the complex.
Q.10 – Which of the followings is not an intermediate of the citric acid cycle?
a) Acetoacetate
b)Citrate
c) Oxalo succinate
d) Succinyl-CoA
a) 2-phosphoglycerate
b) Glucose
c) Phosphoenolpyruvate
d) Pyruvate
a) ATP
b) Cu2+
c) Heme
d) NAD+
a) 3-phosphoglycerate kinase.
b)Glucose 6-phosphatase.
C) Hexokinase.
d)Phosphofructokinase-1.
a)Pyruvate dehydrogenase
b) Isocitrate dehydrogenase
a) Malate dehydrogenase
b) Pyruvate carboxylase
c) Pyruvate kinase
d) Citrate synthase
Q.18 – It is very important to feed the baby very soon after birth, because
during the first few hours after birth the enzyme Phosphoenolpyruvate
carboxykinase is present in very low amounts, and this fact compromises:
a) Gluconeogenesis
b) Glucose phosphorylation
c) Glycogenesis
d) Glycogenolysis
a) Fluoroacetate
c) Malic acid
d) Fluorouracil
b) Phosphorylase
c) Amylo-1,6-glucosidase
d) Glucose-6- phosphatase
b) Concomitant Scurvy
c) Diabetes
b) Acetyl co A carboxylase
c) Transaldolase
d) Succinyl-co A Thiokinase
a) FMN
b) Biotin
c) NAD+
d) TPP
a) Glucose-6 phosphate
b) Fructose 1, 6 bisphosphate
c) Fructose-6- phosphate
d) 2, 3 bisphosphoglycerate
a) Hexokinase
b) Aldolase
c) Galactokinase
Q.6- All the following enzymes except one require NADP+ as a coenzyme-
d) Glucose-6-phophatase
a) Propionyl Co A
b) Acetyl CoA
c) Aceto acetate
b) Pyruvate to Lactate
a) Simple diffusion
Q.10- Which one of the following compounds is common to both the oxidative
phase and the non- oxidative phase of the pentose phosphate pathway?
a) Xylulose-5- phosphate
b) Glucose-6-phosphate
d) Glyceraldehyde-3- phosphate
a) Hepatic hexokinase
b) Muscle Phosphorylase
d) Muscle Hexokinase
a) Hepatic Glycogenolysis
b) Gluconeogenesis
c) Muscle Glycogenolysis
a) Glucose
b) Fructose
c) Galactose
d) Mannose
a) Glycine
b) Alanine
d) Glutamic acid
a) Hepatomegaly
b) Splenomegaly
c) Cataract
d) Mental retardation
a) Lactate<———–>Pyruvate
b) Oxaloacetate—->Phosphoenol pyruvate
c) Glucose-6-phosphate—– >Glucose
d) Phosphoenol pyruvate–>Pyruvate
a) Adipose tissues
b) Skeletal muscles
c) Brain
d) Erythrocytes
a) Succinate dehydrogenase
b) Succinate Thiokinase
c) Succinate lyase
d) Succinate Thioesterase
b) SH linkages
a) Glucose-6 phosphate
b) Fructose 1, 6 bisphosphate
c) Fructose-6- phosphate
d) Glycerol – 3- phosphate
b) Transaldolase
c) Transketolase
d) Glucose-6-phophatase
Q.22- The net production of ATP in glycolysis during anaerobic condition is:
Q.23- Which of the followings does not generate free glucose during the
enzymatic breakdown of glycogen in skeletal muscles?
b) α-1-6-amyloglucosidase
c) Debranching enzyme
d) glucose-6-phosphatase
Q.24- Which of the followings promotes glucose and amino acid uptake by
muscle?
a) Adrenaline
b) Insulin
c) Glucagon
d) Cortisol
Q.25- In a tissue that metabolizes glucose via the pentose phosphate pathway,
C-1 of glucose would be expected to end up principally in:
a) Carbon dioxide
b) Glycogen
c) Phosphoglycerate
d) Pyruvate
1) The answer is –b) Beta hydroxy Butyrate, a ketone body. Ketone bodies serve as alternative
fuel for brain during prolonged fasting or starvation. Fatty acids due to long hydrophobic chain
can not cross blood brain barrier. Glycerol is a substrate of gluconeogenesis. In fact during
prolonged fasting this is the only substrate left to provide glucose through pathway of
gluconeogenesis. It can be oxidized through glycolysis after phosphorylation. Beta carotene is a
provitamin; it is not a source of energy.
2) The answer is –a) Fatty acid break down provides Acetyl co A that serves as a precursor for
ketone bodies. In Diabetes Mellitus glucose utilization is impaired due to absolute or relative
insulin deficiency. Fatty acid breakdown occurs to provide energy and the resultant excessive
Acetyl co A enters the pathway of ketogenesis. Protein breakdown provides amino acids, 6
amino acids are ketogenic, while 14 are glucogenic. Hence protein breakdown contributes only
3) The answer is –a) Galactosemia. The clinical manifestations are typical of classical
Gala tose ia. Bilate al ata a t ules out the possi ilit of Vo Gie ke s disease a d hereditary
fructose intolerance, although other symptoms are there in both these diseases. In juvenile
diabetes mellitus, jaundice and hepatomegaly are not observed.
4) The answer is-d)- Lactate, the end product of glycolysis in erythrocytes and during intense
exercise in skeletal muscles ,is mobilized through Cori cycle to liver to provide glucose by the
process of gluconeogenesis. (Erythrocytes lack mitochondria so the end product of glycolysis is
always lactate. The mode of glycolysis during intense exercise is anaerobic; hence lactate is
formed as a result of glycolysis.
Alanine is transported to liver through Glucose Alanine cycle. Glycerol is also similarly
transported but not from the erythrocytes or skeletal muscles, rather from the adipose tissues.
Glycerol is a waste product in adipose tissues since without phosphorylation it can not be
utilized and the phosphorylating enzyme glycerol kinase is absent in adipose tissues.
5) The answer is-b) Branching enzyme. During the process of glycogen synthesis, branching
enzyme creates branch points and further elongation is carried out by Glycogen synthase . In its
deficiency stored glycogen is abnormal in chemistry, in the form of long polysaccharide chains
with few branch points, resembling the structure of Amylopectin, thus this defect is also called
Amylopectinosis. Alpha Amylase is an enzyme for digestion of starch and glycogen. Debranching
enzyme deficiency results in the accumulation of abnormal glycogen, There is inability to
remove the branch points, the resultant structure resembles Limit dextrin , thus it is also called
Limit dextrinosis
6) The answer is-d) The hydrolysis of starch is catalyzed by salivary and pancreatic amylases,
which catalyze random hydrolysis of alpha (1- 4) glycoside bonds, yielding dextrins, and further
hydrolysis yields a mixture of glucose, maltose, isomaltose (from the branch points in
amylopectin) and maltotriose.
7) The answer is- c) Palmitate, a fatty acid with 16 carbon atoms, is not a substrate for
gluconeogenesis. Even chain fatty acids, predominantly present in our body, yield Acetyl co A
upon oxidation, which can not contribute towards gluconeogenesis. The Pyruvate to Acetyl co
A conversion is irreversible and moreover both of the carbon atoms of Acetyl co A are lost in
the TCA cycle in the form of CO2.Oddchain fatty acid do act as substrates of gluconeogenesis,
since propionyl co A the product of their oxidation can enter TCA cycle through formation of
Succinyl co A, hence can contribute towards Glucose production.
9) The answer is- a) Hexokinase is a non specific enzyme, it can phosphorylate fructose as well
as other sugars but it has high km(low affinity) for fructose. Glucose is the true substrate for
this enzyme.Fructose-6-phosphatethe end product of Hexokinase reaction can enter glycolytic
pathway to be utilized further, so it does not accumulate to produce the toxic effects. Liver
Aldolase (Aldolase B) cleaves Fructose-1-P only, the product of fructokinase catalyzed
reaction. Aldolase A, present in all the cells of the body cleaves Fructose 1,6 bisphosphate, the
product of PFK-1 catalyzed reaction of glycolysis.
10) The answer is- b) Free glucose is released by the action of α-1-6-amyloglucosidaseenzyme,
a component of debranching enzyme. Debranching enzyme has two components. α-[1 4] -α-
[1 4] Glucan transferase and α-1-6-amyloglucosidase.Glucan transferase shifts the trisaccharide
on a branch bound by α-[1- 4] linkage to the straight chain and joins by α-[1 4] linkage. The
exposed branch point is hydrolyzed by α-1-6-amyloglucosidase enzyme. Both components are
present on the same polypeptide chain. Glucose-6- phosphatase does produce free glucose but
it is absent in skeletal muscles.
1) A 30-year-old man has been fasting for religious reason for several days. His brain has
reduced its need for glucose by using which of the following substances as an alternate source
of energy?
a) Fatty acids
c) Glycerol
d) Beta carotene
2) A 7-year-old girl is brought to the emergency department by her parents with complaints of
severe polyuria and Polydipsia. Laboratory examination reveals ketones in her urine. Which of
the followings is the most likely source of ketones?
c) Glycogenolysis
d) Gluconeogenesis.
a) Galactosemia
b) Von-Gie ke s disease
4) The major metabolic product produced under normal circumstances by erythrocytes and by
muscle cells during intense exercise is recycled through liver in the Cori cycle. The metabolite is-
a) Oxaloacetate
b) Alanine
c) Glycerol
d) Lactate
5) A 3-month-old infant presents with hepatosplenomegaly and failure to thrive. A liver biopsy
reveals glycogen with an abnormal, amylopectin like structure with long outer chains and
missing branches. Which of the following enzymes would most likely be deficient?
a) Alpha Amylase
b) Branching enzyme
c) Debranching enzyme
d) Glycogen phosphorylase
6) Prior to a race, many marathon runners will try to increase their glycogen concentrations by
loading up with foods with a high starch content, such as pasta. Alpha amylase secreted by the
pancreas will digest the starch into which of the following major products?
a) Alanine
b) Glutamate
c) Palmitate
d) Pyruvate
8) Which of the following complications is less likely to occur in type II diabetics, as opposed to
type I diabetics?
a) Retinopathy
b) Weight gain
c) Cardiovascular disease
d) Hypoglycemic coma
10) Which of the followings generates free glucose during the enzymatic breakdown of
glycogen in skeletal muscles?
a) Phosphorylase
α-1-6-amyloglucosidase
c) Debranching enzyme
d) Glucose-6-phosphatase