Wavelength, nm %T A

380 90.6 0.0428718023

400 95.5 0.0199966284
420 96.8 0.0141246427
0.14
440 96.7 0.0145735259
460 95.3 0.0209070994 0.12
480 89.8 0.0467236633
0.1
500 82.4 0.0840727883
520 76 0.1191864077 0.08

Absorbance
540 75.1 0.124360063
0.06
560 83.6 0.0777937226
580 90.9 0.0414361168 0.04
600 96.5 0.0154726867
0.02
620 97.3 0.0118871597
640 97.8 0.0096611452 0
660 98.3 0.0074464822 340 390 440 490 540 590 6
680 98.8 0.0052430554 Wavelength, nm
700 99.3 0.0030507515

Lamda Max 525.5

440 490 540 590 640 690 740
Wavelength, nm
Concentration, M %T A
0 100.0 0
0.000008 97.2 0.012334 0.14
0.00002 93.1 0.03105
0.00004 86.4 0.063486 f(x) = 1643.7959085206x - 0.0010384092
0.12 R² = 0.9996901795
0.00006 79.9 0.097453
0.00008 73.9 0.131356 0.1

1000.0 0.08

Absorbance
Sample 99.3 0.003051
0.06

0.04

0.02

0
0 0.00001 0.00002 0.00003 0.00004 0.00005 0.00006 0
Concentration, M
0.0010384092

0003 0.00004 0.00005 0.00006 0.00007 0.00008 0.00009

Concentration, M
Wavelength, nm %T A
380 98.6 0.0061230851
400 99.3 0.0030507515
420 98.9 0.0048037084
0.45
440 98 0.0087739243
460 95.3 0.0209070994 0.4
480 88.5 0.0530567293 0.35
500 79.2 0.1012748184 0.3
520 67.9 0.1681302257

Absorbance
0.25
540 56.6 0.2471835688 0.2
560 48.7 0.3124710388
0.15
580 43.6 0.3605135107
0.1
600 40.8 0.3893398369
620 40.3 0.3946949539 0.05
640 41.7 0.379863945 0
660 44.6 0.3506651413 340 390 440 490 540 590 6
680 48.5 0.3142582614 Wavelength, nm
700 53.1 0.2749054789
440 490 540 590 640 690 740
Wavelength, nm
Concentration, M %T A
0 100.0 0
0.0008 89.2 0.049635 0.45
0.002 80.5 0.094204
0.004 64.0 0.19382 0.4
f(x) = 48.4220219119x + 0.002087748
0.006 51.6 0.28735 0.35 R² = 0.9985672115
0.008 40.3 0.394695
0.3
DF 10.0
0.25

Absorbance
Sample 60.4 0.218963
0.2

0.15

0.1

0.05

0
0 0.001 0.002 0.003 0.004 0.005 0.006
Concentration, M
002087748

003 0.004 0.005 0.006 0.007 0.008 0.009

Concentration, M