{AN RIN — 25 B.Sc. (Semester — VI) Examination, April 2014 CHEMISTRY (Paper — IV, 6 Units) Analytical Chemistry (Optional) Duration : 2 Hours Max. Marks :80 Instructions: 1) Answers to the two Sections to be written on separate answerbooks. 2) Allquestions are compulsory. 3) Figures to the right indicate full marks. 4) Use of non-programmable calculators are allowed. Physical constant : C=3x10' msec h=6.625x10™ ergs-sec SECTION-I (40 Marks) 1. Answer any four of the following : (4x4=16) 1) Explain the deviations of Beer-Lambert’s law. II) Describe the working of a photomultiplier tube. Ill) Explain the sources of radiations used for obtaining ultra-violet visible spectroscopy. !V) Discuss ascending paper chromatography. V) Explain the basic instrumentation features of HPLC. VI) Explain the working of Flame lonization detector. 2. A) |) With aneat labelled diagram explain the principle and working of double beam colorimeter. 4 Il) Acuvette 2 centimeter path length contains a solution of concentration 6.4x10° M. Ifthe transmitted light is 75% of the incident light of wavelength 720 nm, calculate the molar extinction coefficient at this wavelength. 2 OR P10.RIN-25 2 {RR A) Ill) Give four points of distinctions between colorimeters and ‘spectrophotometers. 4 1V) Asolution of a substance with molar absorptivity 8500 gives an absorbance of 0.85 using a cell of 2 centimeters. Calculate the concentration and the percentage transmittance of the solution. 2 B) 1) Write anote on thin layer chromatography. 4 Il) With reference to gas chromatography, what is the function of the carrier gas reservoir. 2 3. A) 1) _ Explain the principle of ion exchange chromatography. 4 ll) With reference to chromatography process, what is meant by retention time. OR A) Il!) Explain the basic principles of gas chromatography. 4 1V)_ Give two applications of gas chromatography. 2 B) 1) With reference to column chromatography explain column adsorbent and preparation of column. Il) Whatis ion exchange capacity ? 2 SECTION-II (40 Marks) 4. Answer any four of the followings : (4x4=16) i) Whats rectifier ? With the help of circuit diagram, explain diode as full wave rectifier. ii) Discuss the construction of SCR and Triac. iii) Explain the principle of thermogravimetric analysis and the nature of the thermogram obtained. iv) Discuss the chemiluminescence method of determining NO-NO, in air. v) Whats fluorimetry ? Uranium content in the sample gives 23 intensity units. The fluorescence calibration curve for the uranium metal has a slope of 6 intensity units per milligram per litre. Find the uranium content in the sample. vi) Give the significance of dissolve oxygen in water. Explain Winkler’s method of determining D.O.(AOU + RIN -25 5. A) i) Discuss the advantages and limitations of Neutron Activation Analysis (NAA). 4 ii) What are fluorescent indicators ? 2 OR iii) 100 mg of the radioactive glycine with a specific activity S, = 30 counts per milligram per minute is mixed with a amino acid mixture. 15 mg of the mixture is isolated and its specific activity is found to be S, = 8 counts per milligram per minute. Determine the amount of glycine in a mixture of amino acid. 4 A) iv) List the type of physical changes that can yield exothermic and endothermic peaks in differential thermal analysis. B) i) Discuss the basic components involved in the instrumentation of TGA. ii) Explain a method of determination of HS in air. 6. A) i) Discuss the n-channel FET. What is pinchoff potential ? yb FM ED ii) What are linear integrated circuits ? OR iii) What is an operational amplifier ? Give the general characteristics of operational amplifier. 4 A) iv) Convert : 108 to a binary number. 2 B) i) Define the terms : 4 a) Fluorescence b) Delayed fluorescence c) Singlet state d) Triplet state ji) Describe the method of analysis of phosphate. 2