BS-411 No.

of Printed Pages – 2 BS-411

(c) Describe regulation of gene expression in prokaryotes with special Roll No.
emphasis on Lac operons.

M.Sc. (BIOTECHNOLOGY / BIOCHEMISTRY)

4. Attempt any Two of the following questions: 7½ x 2 = 15
SECOND SEMESTER EXAMINATION, 2015-16
(a) Describe properties of genetic codons with appropriate examples.
GENE EXPRESSION & REGULATION
How does degeneracy of codon help in evolution? Explain with
suitable justification.
Time : 3 Hours Max. Marks : 75
(b) Describe Structure and function of ribosome. Explain reactions
catalyzed at A, P and E sites of ribosome. Note : (i) Attempt ALL questions.
(ii) Choices are given in each question set.
(c) Write the significance of alternative splicing by giving a suitable
1. Attempt any Three of the following questions: 5 x 3 = 15
example.
(a) Describe salient features of promoter sequences of Class-II genes.

5. Attempt any Two of the following questions: 7½ x 2 = 15 (b) Describe steps of spliceosome mediated splicing mechanism of pre

(a) Explain the basics of protein folding. mRNA.

(b) Explain RNA editing with suitable example. (c) Discuss the role of sno RNA in rRNA processing.

(c) Describe regulation of gene expression of Ara operon. (d) Write short notes on various DNA binding motifs.

2. Attempt any Three of the following questions: 5 x 3 = 15

(a) Discuss the role of different transcription factors in RNA polymerase
II mediated transcription.
(b) Describe the post translational modification in eukaryotes.
(c) Describe the types and function of Amino acyl tRNA synthetase.
(d) Explain the steps and significance of Intein splicing.

3. Attempt any Two of the following questions: 7½ x 2 = 15

(a) Describe steps of addition of 5' cap in eukaryotic mRNA.
(b) Elaborate role of initiation factors and release factors during
translation in prokaryotes.

2 1 P.T.O.