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PERFORMANCE MATERIALS

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Reagents for Sysmex analyzers
Sysmex SF 3000
tWBC + 5 part differential analysis
Application Description Product Nr. Pack size
Diluent Diluid 100 Plus 3961 20 L

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Lysing reagent CyMet 1000 CN Free + 3744 5L
CyMet SF Baso + 3480.5000PC 5L
CyMet SF Diff 1 + 3481.5000PC 5L
CyMet SF Diff 2 3482.0500PE 500 ml

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Cleaning HypoChlorite 1.4% 3941.10000PE 1L
Hematology Control SF Diff Control L/N/H 3693/3694/3695 4.5 ml

Sysmex K series K4500

tWBC + 3 part differential analysis
Application Description Product Nr. Pack size

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Diluent Diluid 100 Plus 3961 20 L
Lysing reagent CyMet 4500 CN Free 3773.5000PC 5L
Emergency Cleaning Hypochlorite 0.5% 3917 1L
3917.5000 5L
Hematology Control 8 Parameter Control L/N/H 3427/3428/3429 2.5 ml
3463/3464/3465 2.5 ml, pierceable

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3701/3702/3703 4.5 ml
K-Diff Control L/N/H 3455/3456/3457 2.5 ml

Sysmex K series 1000

tWBC + 3 part differential analysis

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Application Description Product Nr. Pack size
Diluent Diluid 100 Plus 3961 20 L
Lysing reagent CyMet 1000 CN Free 3744 5L
Emergency Cleaning Hypochlorite 0.5% 3917 1L
3917.5000 5L

sysmex
Hematology Control 8 Parameter Control L/N/H 3427/3428/3429 2.5 ml
3463/3464/3465 2.5 ml, pierceable
3701/3702/3703 4.5 ml
K-Diff Control L/N/H 3455/3456/3457 2.5 ml

Sysmex K series KX21

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tWBC + 3 part differential analysis
Application Description Product Nr. Pack size
Diluent Diluid 100 Plus 3961 20 L
Lysing reagent CyMet KX CN Free 3425.0500 500 ml
Emergency Cleaning Hypochlorite 0.5% 3917 1L
controls

3917.5000 5L
Hematology Control 8 Parameter Control L/N/H 3427/3428/3429 2.5 ml
3463/3464/3465 2.5 ml, pierceable
3701/3702/3703 4.5 ml
K-Diff Control L/N/H 3455/3456/3457 2.5 ml
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Cross list: Sysmex reagents vs J.T.Baker

Toa Sysmex S Nr. J.T.Baker JTB Nr.
Cellpack (20 L) 884-0871-1 Diluid 100 Plus 3961
Cellpack GL (20 L) 884-1271-1
Cellent (20 L) 824-0043-0 Diluid 100 Plus 3961
Cellkit CD (500 ml) 784-0032-0
Cellsheath (20 L) 834-0032-0
Stromatolyser FB CyMet SF Baso 3480.5000PC
Stromatolyser FD-I CyMet SF Diff 1 3481.5000PC
Stromatolyser FD-II CyMet SF Diff 2 3482.0500 PE
Stromatolyser-3WP (5 L or 3 x 500 ml) 854-0011-7 CyMet 1000 CN Free or 3744
874-0331-9 CyMet 4500 CN Free 3773
Stromatolyser-C (5 L or 3 x 500 ml) 834-0371-6 CyMet 1000 CN Free or 3744
874-0361-1 CyMet 4500 CN Free 3773
Sulfolyser (5 L) J0411511 CyMet 1000 CN Free or 3744
CyMet 4500 CN Free 3773
Stromatolyser-WH (500 ml) CyMet KX CN Free 3740
Rhemox-W (3 x 250 ml) 784-0142-1 Lyzerglobin (500 ml) 3077
Rhemox-3WP (3 x 500 ml) 884-0151-6
Rhemox-PDA (3 x 250 ml) 824-0012-4
Rhemox-M (3 x 250 ml) 874-0161-0 Lyzerglobin (6 x 15 ml) 3769
Quicklyser-Hb (3 x 250 ml) 804-0172-2 CyMet Automated or 3755
CyMet Automated CN Free 3757
3780
Quicklyser-QLY (50 x 6.0 ml or 50 x 1.5 ml) Lyzerglobin or 3769
Lyzerglobin Cyanide Free 3505
Quicklyser II 50 x 6.0 ml) 884-0681-7 Lyzerglobin II 3437
Cellclean (50 ml) 834-0161-8 Hypochlorite 0.5% or 3917
ProClean Plus 3901
Pash (5 L) 804-0082-4 ProClean 3900
Manoresh (5 L) 804-0062-0 Detectoterge or 3763
ProClean 3900
Eightcheck 8 parameter control blood
Cellcheck-400 (50 x 10 ml) 814-0022-6 8 parameter control blood
Placheck-100 (10 x 2 ml) 784-0102-2 Platelet Control Extended Value 3424

Consumption of reagents
Type Reagents Consumption per test
K 1000 Diluid 100 Plus 30 ml
CyMet 1000 2 ml
(Hb and WBC lytic reagent)
ProClean Plus required to remove clogs
Hypochlorite
K4500 Diluid 100 Plus 30 ml
CyMet 4500 2 ml
(Hb and WBC lytic reagent)
ProClean Plus required to remove clogs
Hypochlorite
KX-21 Diluid 100 Plus 34 ml
CyMet KX 1 ml
Diluid, CyMet and ProClean are all trademarks of Avantor Performance Materials Inc.

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Extra information CyMet 1000 for Sysmex K1000

The Sysmex K1000 and equivalent instruments operate with a

separate lysing reagent for the WBC differential analysis and the
hemoglobin monitoring.
Opposite Coulter and equivalent instruments, these 2 parame-

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ters are also measured in a separate channel on the K1000. We
have found that there is no reason to have 2 independent
reagents. Both the Hb lyser and the WBC lyser are the same
solution. The J.T.Baker lysing reagent is CyMet 1000, product
number 3744 5L polycube.

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CyMet 1000 replaces both the Stromatolyser 3wp and the Reagent connection to the K1000/K4500.
Sulfolyser. CyMet 1000 should be used in combination with
Diluid 100 Plus.

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sysmex
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controls
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Priming and Calibration of Sysmex Analyzers

Sysmex K1000
Priming of reagents on Toa Sysmex Example
Priming of the Avantor Performance Materials reagents as bits 8 7 6 5 4 3 2 1
described in this protocol is necessary, because the K800/K1000 switch 1 0 0 1 0 0 1 1
has internal reagent reservoirs. When priming is not complete,
contamination of Avantor reagents with Toa reagents will occur.
The priming protocol as described below must be done to 6. Switch bit 7 in the “1” position by pressing first key <6> and
perform a successful priming: then key <8>. Repeat this handling to switch bit 6 in the “1”
position. Press two times <ENTER> to confirm the settings
Priming of K1000 in the PROGRAM SWITCH SW 3.
1. Switch ON the power of the Toa K1000 instrument and
the compressor with the POWER SWITCH. An AUTO RINSE 7. Turn off the Toa K1000 instrument with the POWER
procedure will be carried out. SWITCH. The POWER SWITCH of the compressor unit must
be kept in the switch on position.
2. Go on with step 3 when the background values are within 8. Turn on the Toa K1000 instrument with the POWER
to the following specifications: SWITCH. The WBC, HGB and diluent reagent chamber and
all tubes with reagent will be emptied. After this action
WBC < 0.3 x 109/l the Toa K1000 will automatically perform an AUTORINSE
RBC < 0.02 x 1012/l procedure. When the following error message appears
Hb < 0.1 mmol/l on the printout: NO DILUENT REPLENISH & PRESS 1. Put
PLT < 10 x 109/l first the aspirating tube of the diluent into the Diluid 100
plus cubitainer and then press key <1> to continue. If the
3. Remove the aspirating tubes from the Toa Reagent error message appears again press key <1> to solve this
cubitainers and clean the outside surface of these tubes error. The Avantor Performance Materials diluent is now
thoroughly by rinsing them with distilled or de-ionised introduced in the Toa K1000 instrument.
water. After rinsing put the aspirating tubes in a clean and
empty beaker.
WARNING: DO NOT SWITCH OFF the K1000 instrument.
4. To remove the Toa Reagents from the K1000, PROGRAM
SWITCH SW 3 must be changed. Press <SELECT> <C> <7>
<ENTER> on the keyboard. Select PROGRAM SWITCH by
pressing 3 times the key <2>. The message PROGRAM WARNING: CONVINCE YOURSELVES THAT THE INTERNAL
SWITCH will appear in the display. Then press <ENTER>. REAGENT RESERVOIRS OF THE HGB AND WBC
REAGENT ARE COMPLETELY EMPTY.
5. Press two times the <ENTER> key to activate the
PROGRAM SWITCH SW 3 is activated. You can see it visually by taking of the front cover and
PROGRAM SWITCH SW 3 consists of the following look into the reservoirs. If there is still some reagent in the
functions: reservoirs, repeat step 4 to 7 again, until the reservoirs are
Bit No. Function completely emptied.
8 Not in use
9. The Toa Reagents are removed from the K1000. The
7 6 Service Purpose only. Avantor Lytic Reagent CyMet 1000 must be introduced.
These bits should be in the “0” position. Connect the aspirating tube of the WBC Reagent and HGB
Reagent with the Y-joint as showed in the picture below.
5 4 Select the date format. One of the aspirating tubes is now redundant and will not
Bit 5 4 = 0 0 (yy/mm/dd) be used anymore.
0 1 (mm/dd/yy) It’s also possible to connect CyMet 1000 cubitainers to the
1 0 (dd/mm/yy) WBC and HGB aspiration tube, without using the Y-joint.
1 1 (yy/mm/dd)
aspirating tube aspirating tube
3 2 1 Select the units of HGB REAGENT of WBC REAGENT
Bit 3 2 1 = 0 0 0 (Japanese)
0 0 1 (General export units)
0 1 0 (Standard SI)
0 1 1 (Dutch SI)
1 0 0 (Canadian SI)
1 0 1 (Hongkong SI)
1 1 0 (Japanese)
1 1 1 (Japanese)
CyMet 1000

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10. Put the combined aspirating tube into the cubitainer of
CyMet 1000.
11. Perform an AUTO RINSE by pressing the keys <SELECT>
<C> <4> <ENTER>. If there is a CAP PIERCING UNIT on the
Toa K1000 you must press key <1> after these commands
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instrument. If change of the HGB calibration value is not
necessary, press <ENTER> without pressing numeric keys.
Then the cursor will move to the HCT calibration value. Set
the HCT calibration value in the same way.
4. After setting of HCT calibration value by pressing <ENTER>,

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for the OPEN MODE or key <2> for the CLOSED MODE. the built in printer prints date, old calibration values and
new calibration values and the instrument returns to the
12. Within a few minutes the following error message appears ready mode.
on the printout: NO HGB REAGENT REPLENISH & PRESS 1.
Press key <1> and the CyMet 1000 will be aspirated in the Toa Example

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K1000 and the HGB buffer chamber will be filled. If the error CALIBRATION ACCEPTED
message appears again press key <1> to solve this error. 15/10/97 09:20
HGB : 117.0%  119.8%
13. The next error message on the printout will be: NO WBC HCT : 104.0%  105.0%
REAGENT REPLENISH & PRESS 1 Press key <1> and the CyMet
1000 will be aspirated in the Toa K1000 and the WBC buffer NOTICE THAT HGB AND HCT FACTORS CAN BE CHANGED
chamber will be filled. If the error message appears again press WITH STEPS OF MAXIMUM 5%. IF A HIGHER CALIBRATION IS

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key <1> to solve this error. REQUIRED, THIS CAN BE DONE WITH SOME STEPS.
The AUTO RINSE procedure will be completed.
Example
14. Perform another AUTO RINSE procedure by pressing HGB : 100.0% to 108.0%
the keys <SELECT> <C> <4> <ENTER> and check if First enter the value 104.0% and then the second time
the background values are within the specifications 108.0% (2 times 4%)
as mentioned in point 2 of this protocol. When the A calibration of >5% at once will be ignored.

background values are too high perform another AUTO
RINSE procedure.
Note: After priming the background value of WBC may be
increased. This is mainly due to air bubbles in the system.
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5. If it’s necessary to calibrate the other parameters: WBC,
RBC and PLT. Press first <C> <9> <.> <0> to go in the
maintenance menu and then press <SELECT> <C> <8>
<ENTER>.

In this case, perform the next handling:
- Press <SELECT> <C> <6> <ENTER>, all counting
chambers will be drained.
- Press the <START> BAR to perform a blank count.
- Repeat this a few times. The blank counts will slowly
drop to acceptable values.
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6. Press key <2> or < >until C.O.C. appears in the LCD. Press
<ENTER> to activate this option.
7. If calibration is required, change only the first WBC, RBC
and PLT calibration values. Calibration values can be

sysmex
- If high background values still remain, turn the power changed by pressing numeric keys, followed by <ENTER>.
off and wait for 30 minutes. Turn the power on and the DO NOT change the calibration values with suffix (C) or (P).
K1000 will run an AUTO RINSE procedure.
8. With the option <PRESET DATA> in the <SELECT> <C>
Note: When changing from Cyanide to Cyanide free <8> <ENTER> menu you can print out all calibration data.
reagents a recalibration for the Hb is required.

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Example
15. All Avantor Reagents are now introduced in the Toa K1000. CALIBRATION DATA
Check the priming by analysing five times one EDTA blood WBC : 85.6%
sample. If the WBC and Hb values remain constant the RBC : 104.5%
priming was successful. If these values aren’t constant, PLT : 96.0%
controls

perform another AUTO RINSE.

WBC : 127.7% (P)
Calibration of Toa Sysmex K1000 Analyzers RBC : 100.4% (P)
1. Press the SELECT key in the ready mode, and select the PLT : 108.0% (P)
CALIB. HGB/HCT program by using the cursor key (“2” HGB : 100.1% (P)
key or “8” key ). Then press the ENTER key to execute the MCV : 99.8% (P)
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CALIBRATION HGB/HCT program. <SELECT> <C> <5>

<ENTER> will give the same action. WBC : 97.0% (C)
RBC : 105.8% (C)
2. The LCD will display the current calibration value. The left PLT : 114.6% (C)
value is HGB calibration HGB : 79.3% (C)
value and the right HCT calibration value. MCV : 94.3% (C)
3. The HGB calibration value which is on the cursor can be
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changed. Input a new setting value with numeric keys and 9. After calibration, when in the ready mode, press <C> <0>
press <ENTER> to set the new calibration value into the to get out of the maintenance menu.
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Sysmex K4500
Priming of K4500
1. Take the aspirating tubes from the Sysmex reagent
cubitainers. Don’t lay the tubes on tissues or dusty surfaces.
Place them in clean beakers.
2. It is important to rinse the outside surface of the aspirating
tubes with distilled or de-ionised water to be aware of any
kind of reagent contamination.
3. Open the front cover of the instrument by turning the
cover upwards.
4. Unscrew the screws on the left top and left bottom. These
screws lock the left side panel.
s K4500
5. When the left side panel is opened, the internal reagent
chambers can be found on top. There are three reagent
reservoirs: the one on the left is the WBC-lyse chamber, the
one in the middle the Hgb-lyse chamber and the larger
one on the rigth is the diluent reagent chamber.
6. Unscrew these internal reagent chambers and waste the
reagents inside.
7. Screw the reagent chambers on again. Press AUTORINSE.
The remaining reagents in the aspiration tubes are
aspirated into the reagent chambers. Discard any error on
the LCD screen. Press the C key to deactivate the audio
beep.
8. Unscrew these internal reagent chambers again and
waste the reagents inside for the second time. All original
reagents are wasted now.
9. Screw the reagent chambers on again.
10. Connect Avantor Performance Materials reagents to the
aspiration tubes.
11. Press AUTORINSE. The following error message appears on
the print out: ! ERROR ! DILUENT ERROR REPLENISH AND
PRESS AUTORINSE KEY TO RETRY.
12. Press AUTORINSE. The next error message appears on the
print out: ! ERROR ! HGB REAGENT ERROR REPLENISH AND
PRESS AUTORINSE KEY TO RETRY.
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PERFORMANCE MATERIALS
13. Press AUTORINSE. The next error message appears on the
print out: ! ERROR ! WBC REAGENT ERROR REPLENISH AND
PRESS AUTORINSE KEY TO RETRY.
14. Press AUTORINSE. All Avantor reagents are aspirated into
the reagent chambers now.
15. After the Autorinse procedure is finished, the background
values are printed out. Verify if these values are acceptable
within limits. If not, press AUTORINSE again for another
cleaning/priming procedure. Verify the background values.
Calibration of Toa Sysmex K4500 Analyser
1. Press the <SELECT> key in the sample analysis mode.
2. Then press < >, until menu 4. CALIBRATION.
3. Press <ENTER> twice. In this menu only the calibration
factors for Hemoglobin (HGB) and Hematocrit (HCT) can
be altered.
4. If values needs to be corrected press <C> <.> To confirm
press <ENTER>
5. Set HGB/HCT calibration value by pressing numeric keys
followed by <ENTER>.
NOTICE THAT HGB AND HCT FACTORS CAN BE CHANGED
WITH STEPS OF MAXIMUM 5%. IF A HIGHER CALIBRATION IS
REQUIRED, THIS CAN BE DONE WITH SOME STEPS.
Example
HGB : 100.0% to 108.0%
First enter the value 104.0% and then the second time
108.0% (2-times 4%)
A calibration of >5% at once will be ignored.
6. Finally press <SELECT>. In the LCD next message will
appear:
CHANGE SETTINGS Y:ENTER / N: SELECT
7. Press <ENTER> or <SELECT>
8. Press <SELECT>. New calibration factors will be printed
automatically.
9. To calibrate other factors press <C> <9> <.> <0>. (Service
mode)
10. Then press <SELECT> and select with the cursor keys
menu 6 <SERVICE PROGRAM> and press <ENTER>.
11. Select menu 3 <Service Calibration> with cursor keys and
press <ENTER>. Press <PRINT>. In this menu the calibration
factors for WBC, RBC, HGB, MCV and PLT can be altered.
12. Change factors if necessary. Go to next factor by pressing
<ENTER>
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Sysmex KX-21
Priming of KX-21
1. Take the aspirating tubes from the Sysmex diluent 5. Finally press <SELECT>. In the LCD next message will
cubitainer. Don’t lay the tube on tissues or dusty surfaces. appear:
Place it in a clean beaker. CHANGE SETTINGS
Y:ENTER / N: SELECT

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2. Open the front cover of the instrument at the left side
and turn the cover to the right. The lyse bottle is located 6 Move to Yes with > and < press <ENTER>
behind the front cover. Take the aspirating tube from the
Sysmex lyse bottle. Don’t lay the tube on tissues or dusty 7. Press <SELECT>. New calibration factors will be printed
surfaces. Place it in a clean beaker. automatically.

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NOTICE THAT HGB AND HCT FACTORS CAN BE CHANGED
WITH STEPS OF MAXIMUM 5%.
IF A HIGHER CALIBRATION IS REQUIRED, THIS CAN BE DONE
WITH SOME STEPS.

Example

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HGB: 100.0% to 108.0%
First enter the value 104.0% and then the second time
108.0% (2-times 4%)
A calibration of >5% at once will be ignored.

8. To calibrate other parameters press <C> <9> <.> <0>.

KX-21 Reagents: (service mode)

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1. Diluent (Cellpack)
2. Lyse (Stromatolyser-WH) 9. Then press <SELECT>, <9. SERVICE>, <3. SETTINGS>, <2.
CHANGE>,
3. Press on numeric keyboard <SELECT>. Then select <5. <1. CALIBRATION>.
AUTO RINSE> key.

4. When the instrument gives an alarm, which indicates no
reagents, then connect Avantor reagents to the aspiration
tubes.
Sysmex Baker
Cellpack Diluid 100 Plus
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10. Using the ,  and <ENTER> keys, move the cursor to
the parameter you wish to change. Using the numeric keys
and decimal key, enter the calibration value.
11. With the cursor on the bottom line, press the  key will
change the displayed parameters.

sysmex
Stromatolyser WH Cymet KX-21
12. If you press the <SELECT> key, the change confirmation
5. Then press the <5. AUTO RINSE> key again. Reagents will message will appear in the menu display area
be primed. If you select <CONT>, you can continue to enter the
6. Repeat step 3-times. calibration values.
If you select <SET>, the calibration value will be updates

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7. The lyse can be separately primed by using next steps: and the system will return to the READY screen.
– Press <SELECT>
– Then press <4. REPLACE LYSE> If you select <CANCEL> or press the <SELECT> key, the
system will return to the READY screen without changing
Calibration of Toa Sysmex KX-21 Analyzer the settings.
controls

1. Press the <SELECT> key in the sample analysis mode.

2. Then press <3> to enter <3. CALIBRATION>. Sysmex HGB (Hemoglobin) Adjustment
Procedure for Sysmex KX-21
3. Press <2> <ENTER> to select
<2. MANUAL CAL.>. In this menu only the calibration Problem Description:
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factors for Hemoglobin (HGB) and Hematocrit (HCT) can
be altered.
4. Set HGB/HCT calibration value by pressing numeric keys
followed by <ENTER>.
Sometimes customers run into Hemoglobin calibration
problems on the Sysmex KX-21 instruments when switching
from Original Sysmex reagents to JT Baker reagents. Due
to the combination of Diluid 100 Plus and CyMet Kx,
the Methemoglobin complex is measured differently in
comparison to the original reagents. This sometimes results in
calibration problems, where calibration factors are calculated
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for Hgb outside the possible enterable ranges.

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Problem Solution:
In the service manual (page 4-5) a procedure is explained how
to adjust the HGB reference blank, but more important the HGB
conversion measured electronically. By means of a potential
meter (potmeter) the HGB conversion can be changed (and
directly influenced the HGB value measured) in a way that no
calibration has to be done or that a calibration is again possible
within the enterable ranges.
13. Adjust VR2 (HGB SAMPLE) on the PCB No. 2135 clockwise
to increase the HGB value measured. One full turn
clockwise or counterclockwise will change the HGB value
around 0.7 g/dL.
14. Aspirate sample 1 and check again the value measured.
Adjust VR2 until the required HGB value is measured.

Procedure: 15. Check the HGB Convert BLANK value again and adjust VR1
1. Run 5 samples with the original Sysmex reagents for if needed (see step 9).
reference purposes.
16. Press [SELECT] key to resume the sequence.
2. Install J.T. Baker reagents on the analyzer following the
normal procedures.
Sysmex Sysmex TM KX-21 and Sysmex TM
3. Run 5 samples (from step 1) with the J.T. Baker reagents. K4500, high WBC Background
For many years we produce reagents for the 3-part differential
4. Calculate new Calibration factors for CBC parameters that instruments from SysmexTM Company. Initially we started
need calibration producing reagents for the K1000, but more and more we
produce reagents for the K-4500 and KX-21.
5. If possible change calibration factors (Note that HGB and
HCT factors can be changed with steps of maximum 5%). If Feedback from some of our distributors show that sometimes
the calibration factor is out of range, go to step 6. distributors run into problems installing JT Baker’s reagents on
the above instruments. Especially when they switch from other
6. Shutdown the analyzer. manufacturer’s reagents to JT Baker’s reagents, they observe
high WBC Background counts after installation and priming.
7. Disconnect the net cable and remove the Top Cover. Take notice that this problem will not occur on all K-4500 and
KX-21 instruments in the field.
8. Switch on the instrument and startup.
The reason for this increase in WBC Background is the result of a
9. Display the Status Display by pressing [SELECT] – [7] chemical process in the Diluid circuit, Diluid lines and the WBC
(Maintenance) – [5] (Status Display). Counting Chamber.
S *System Display* Due to long use of reagents of other manufacturers a small
layer (film) of coating can be introduced in the Diluid circuit of
SEQ. NO. 12 above instruments and especially in the WBC counting
PRESSURE 0.50
VACUUM 250 chamber. After connection of our reagents and performing the
HGB CONVERT 2000 priming protocol, as described in our Distributor’s Catalogue
SENSOR 1 2 3 4 5 6 7 8
Hematology, this layer slowly dissolves, resulting in an increased
background of the WBC’s after the Autorinse cycle.
SV 1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1 2 3 4 Next procedure explains how you can solve this high WBC
Background problem:
1. Prepare a dilution of 500ml distilled water and 500ml of
Hypochlorite (3917).
10. Adjust the VR1 on the PCB No. 2135 (located in the Top
middle part of the analyzer) so that the HGB CONVERT 2. Follow the procedure to empty the Diluid reagent reservoir
BLANK value falls within the range 2000+/- 200. (only K4500).

3. Connect the hypochlorite solution to the Diluid Line.

4. Perform 3 times an Autorinse.

5. Wait for 30 minutes.

6. Perform another Autorinse.

7. Connect 500ml of distilled water to the Diluid Line.

11. Aspirate sample 1 of step 1 and wait for the printed sample
result. 8. Follow the procedure to empty the Diluid reagent reservoir
(only K4500).
12. Compare the HGB value measured with the reference value.

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9. Perform two Autorinses with distilled water. Priming of SF3000:

10. Connect the Diluid 100 Plus to the Diluid Line. Note:Before starting this protocol, please be sure
that you ran fresh human samples with the original
11. Follow the procedure to empty the Diluid reagent Sysmex reagents. These are necessary for reference and
reservoir (only K4500). calibration. Or use Hematology controls for reference.

12. Perform two Autorinses with Diluid 100 Plus.
13. Run background counts until background counts for WBC,
RBC, HGB and PLT are acceptable. Sometimes more than
backgrounds are necessary.
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1. Take the reagent tubes from the original Sysmex reagents
containers and bottles.
2. It is important to rinse the outside surface of the aspirating
tubes with distilled water or de-ionised water to avoid

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reagent contamination.
Sysmex SF3000
3. The internal reagent reservoirs are connected to the
Introduction of Priming on the SF3000: Fluidic system of the SF3000 with Fluid Connectors, which
Priming of the Sysmex SF3000 consists of a lot of sequential are used in this protocol to empty all separate reagent
steps that have to be performed to avoid micro-bubbles, reservoirs. Next picture shows an example and the pin
cross-contamination of Sysmex and JT Baker reagents and high connections of a Fluid Connector.

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background counts.

The SF-3000 has several internal reagent reservoirs for the 5

reagents connected. It is really advised and necessary to empty the
reagent reservoir using this procedure. To access these reagents
reservoirs, open the left door by removing two screws that are
located on the left of the front panel.

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In the left compartment you can find reagent reservoirs for the
Cymet SF Baso (Stromatolyzer FB), CyMet Diff 1 (Stromatolyzer FDI),
CyMet Diff 2 (Stromatolyzer FDII), Cymet 1000 CN Free (Sulfolyzer)
and three reservoirs for the Diluid 100 Plus (Cellpack), PK1, PK2 and
PK3. In total 7 reagent reservoirs have to be emptied.

swelab
Next table shows an overview of all Fluid connectors,
valves and pin numbers that are used in this priming
protocol.

sysmex
Item Pin Reagent
Nr
Fluid Connector No. 12 1 CyMet SF Baso
Fluid Connector No. 12 2 CyMet SF Diff 2
Fluid Connector No. 12 3 CyMet SF Diff 1

Fluid Connector No. 12
Fluid Connector No. 8
Fluid Connector No. 19
Valve 31
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4 Diluid 100 Plus (PK3)
3 Diluid 100 Plus (PK1)
4 Diluid 100 Plus (PK2)
CyMet 1000
controls

4. Open the left cover and locate the reagent reservoir for the
CyMet SF Baso (see picture on previous page). This is the
reservoir on the left. Follow the tubing coming from the
bottom of the reagent reservoir until you reach the Fluid
Connector No.12. Clamp the tubing that is coming from
cleaners

the CyMet SF Baso reservoir and disconnect the tubing

Priming of the reagents can only be performed if the
instrument is in the “Ready” state. After 10 minutes of non-
usage the Standby mode is activated and the Vacuum/Pressure
will be switched off. Press the Start button to return to the
from the Fluid Connector tubing connector pin 1. Take care
of liquid spills! Place a beaker under the disconnected tube
and open the tubing clamp. CyMet SF Baso reagent will
flush out of the reservoir. Connect the tubing back to the
correct pin number (Watch out: there are some unused
pins on the Fluid connectors!).
pbs

Ready state. If the Vacuum and Pressure are switched off, it is

impossible to empty the reagent reservoirs manually.
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91
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PERFORMANCE MATERIALS

5. Perform the same procedure for the CyMet SF Diff 2 (pin2),
CyMet SF Diff 1 (pin3) and Diluid 100 Plus/ PK3 (pin4)
connected to Fluid connector 12. Be sure to clamp the
tube for Diluid PK3 because of the pressure on the reagent
reservoir.
6. Locate the Fluid Connector 8 and 19 to perform the
same procedure for Diluid 100 Plus, PK1 and PK2 reagent
reservoirs. Clamp the tubing on time after emptying,
otherwise a pressure alarm will follow. Deactivate the alarm
by pushing the Clear Alarm button.
7. Locate the reagent reservoirs for the CyMet 1000 (second
reagent reservoir from the right) and follow the Teflon
tubing coming from the reservoir up to Valve no. 31. Don’t
clamp the tubing. Use tissues and a beaker to avoid liquid
spills after disconnecting.
15. After finishing step 14, go to the Ready Screen and press
again the “Next No.” icon. Enter [C] [0] and check if you have
deactivated the Service mode. Press [More] twice to see if
the extra button [Service] is gone. If not repeat step 15.
16. Press [Auto-Rinse] and wait till the sequence ends and
check the background results. If the instrument gives WBC
and/or Diff errors press [Quit] and repeat the Auto-rinse
sequence until the Backgrounds are ok. Micro-bubbles
cause these Background problems.
17. Run 5 Blanc / Background counts. The WBC should be
lower than 0.05 and the scatter graph should look like
the result below. Sometimes it helps to run some Human
Blood samples between the Blanc / Background counts to
improve the Background counts.

8. After emptying all 7 reservoirs check if all tubing are GOOD

properly connected back to the Fluid Connectors. Press the
“Replace Reagent” button on the screen. The system will try
to fill all reagent reservoirs. The remaining reagents in the
tubing and system will be flushed in the corresponding
reagent reservoirs. Several alarms will follow. Press “Clear
Alarm” until next message appears:

Replace Reagent, and Press EXEC. Key.

9. Repeat steps 4 to 7 to empty the last remaining reagent

from the reagent reservoirs. The CyMet SF Diff 1 reservoir
will fill totally again, because of the length of the tubing
coming from the reagent container. WRONG

10. Connect, after cleaning the outside of the reagent tubing,

JT Baker reagents to the instrument.

11. Push the “EXEC” button. The instrument will fill all reagent
reservoirs with JT Baker reagents. Again several alarms will
sound. Press the “EXEC” button every time the instrument
asks for it. The instrument will go to the READY state if all
reagent reservoirs are filled with reagents.

12. Step 12 is one of the most important steps in the priming

sequence. The lines coming from the reagent reservoirs are
still empty and filled with air. It is necessary to disconnect
every single reagent line from its corresponding Fluid
Connector for 1-2 seconds. If you see reagents coming out
of the tubing, clamp the tubing and connect the tubing
back to the pin connector. Watch out for spills, use clamps.
13. In the Ready mode, press the “Next No.” icon (on the top of
the screen). Enter [C] [9] [-] [0] en [Enter] and check if you
activated the Service mode. Press [More] twice to see if
there is an extra button [SERVICE]. If not repeat step 13.
14. Press [Service], [Special Sequence], [Setting Sequence] and
the “Start Switch”. The instrument will now flush all reagent
pumps, tubing and syringes with the JT Baker reagents.
This will take around 10 minutes.
Calibration of Sysmex SF3000
1. Customers can only calibrate HGB/HCT calibration factors.
Other parameters can only be calibrated in the Service
Mode.
2. Press the [Calib] button in the Ready screen.
3. Choose between the Auto or Manual HGB/HCT calibration.
Please refer to chapter 7 of the operator Manual for further
explanation of the calibration protocol.
4. If other parameters than HGB and HCT have to be
calibrated, the Service Mode has to be entered. In the
Ready mode, press the “Next No.” icon (on the top of the
screen). Enter [C] [9] [-] [0] en [Enter] and check if you
activated the Service mode. Press [More] twice to see if
there is an extra button [SERVICE]. If not repeat step 3.

92
 Medonic
TM

PERFORMANCE MATERIALS

melet schloesing
5. Press [Service], [Change] and [Calib.]. You have entered
the screen where all different Calibration factors for all
parameters can be found. The different measurement
modes are shown.
For example:
WBC-Cal = Manual mode

mindray
WBC-SM = Sampler mode
WBC-Cap = Capilary Mode
WBC-V = ?? Mode.

6. Use the cursor to go to through the calibration factors that

nihon kohden
have to be changed. Use the standard Calibration Method
to calculate new factors. Note: Only the Cal factors for the
Manual mode and Sampler Mode have to be changed.
When both the manual mode and sampler mode are
correctly calibrated towards each other, the same change
in manual mode calibration
factor can be used in sampler mode calibration factor as

orphee
well.

Example:
Reference values Sysmex Reagents:
Manual Mode:
PLT = 280
PLT CAL factor (old) = 98,7%

seac
Sampler mode:
PLT = 275
PLT SM Factor (old) = 96,5%

Measured values JT Baker Reagents:

swelab
Manual Mode:
PLT = 290
New PLT CAL Factor = 280 / 290 * 98.7%
= 95,3 %

New PLT SM Factor = 280 / 290 * 96,5%

sysmex
= 93,2 %

The same change in Manual Mode calibration factor is used for

the Sampler mode calibration factor.

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controls
cleaners
pbs
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93
About Avantor™ Performance Materials
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