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carbonate
Urine
● urine becomes ammoniacal in odor and
- Filtrate from the blood alkaline in pH
- Serves as a medium for the excretion of the ff: ➔ In ALKALINE urine, the ff precipitate:
COLLECTION & PRESERVATION OF URINE SAMPLE ➔ Specific Gravity: range of 1.015 - 1.025
➔ Volume: of 24 hour urine is 1000-1500 ml. ➔ These precipitates are the primary components of kidney
➔ Color: stones.
➔ When urine is allowed to stand for sometime, exposed to - Creatinine - breakdown product of Creatine phosphate
air: (CP)
➔ When urine is allowed to stand after sometime w/o B. Picric acid Reaction (Jaffe)
preservative:
1) 5 mL urine + picric acid (C6H3N3O7)
◆ Oxidation:
2) Alkalinize with NaOH
● Result: Red ppt = due to formation of tautomer 2) Filter off the earthy phosphates and small amount of
of creatinine picrate. magnesia mixture to the filtrate.
- If it does not turn yellow, acetone ● Result: Magnesia mixture precipitates alkali phosphates (Na
bodies are present and K salts).
- Glucose gives a similar color (when heated) 4) The alkali phosphates of Na and K separate.
1) Boil urine
**Normal amt of chlorides eliminated in 24h: 10-15 grams - Perform Benedict's Test : Semi quantitative test.
● Result: Precipitation of earthly phosphates (Ca and Mg ○ Red - conc. amt. of glucose
salts) occur.
○ Yellow - glucose substantially present
- The earthy phosphates / phosphates of Ca and ○ Green - little glucose present
Mg separate.
○ Blue - no color change; no glucose present Ketonuria - presence of these substances in urine
9. BLOOD
2) Cool. Treat with 3 mL of sat. benzidine (C12H12N2) in ● Results: ruby red color = presence of acetone
glacial acetic acid.
3) Add 0.5 mL of acetic acid. Observe further.
3) Add 1 mL of 3% hydrogen peroxide (H2O2).
● red color persists = ketone bodies are present.
● Result: blue/green color = presence of blood
10. BILE
PROCEDURE:
➔ Acetone