Final Year Exam Form 4, 2010

Date : 4 November 2010.

Paper 3 BIOLOGY 4551/3

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Question 1.

1(a) Record the time taken for the methylene blue in water sample Q,S,T.

Water sample Time shown on The time taken for methylene

taken from the stop watch blue to decolorize
village (hour) (hour)

Q 3.0

S 2.0

T 1.5

(b)i …..two observations…..

1. At location Q, the time taken for methylene blue to decolourise is 3 hours

2. At location T, the time taken for methylene blue to decolourise is1.5 hour

(ii) …….Inference ….

1. Water sample Q, the time taken for methylene blue to decolourise is longer
because higher concentration of dissolved oxygen// less polluted/ low
microorganism.// low amount of waste.

2. Water sample T, the time taken for methylene blue to decolourise is ………
because …
c).(i).Complete Table…….

Variable Method to handle the variable

Manipulated variable
Water sample from different location Use different water sample at location
Q,S & T.
Responding variable
Time taken for of methylene blue to By measure & record the time taken for
decolourise. decolourisation of methllene blue by
using a stop watch
Controlled variable
Concentration of methylene blue . By fixing the concentration of of
methylene blue at …….%.

ii) …… classifying the apparatus and material…..

Apparatus Material
Measuring cylinder Water sample

Syringe Methylene blue (0.1%)

Syringe//reagent bottle Methylene blue// water sample

(d) State the hypothesis……

Water sample Q is most polluted//has the lowest

concentration of dissolved oxygen// highest B.O.D value compare to water
sample S & T
or //
Water sample Q takes the longest time to decolourise the methylene blue solution
compared to S andT.

e(i) Construct a table …..

Water Time taken for the Level of water The BOD level
sample methylene blue to pollution
decolourise(hours) 1/ time (hour -1)
Q 3.0 0.33 Low
S 2.0 0.50 Moderate
T 1.5 0.67 High
e(ii)
Time for
methylene
blue to
decolouris
(hour)

Q S T water samples

f) ……. relationship between the time……….

The higher the level of water pollution, the shorter the time taken for the methylene
blue to decolourise because more waste are release into the river / low oxygen content /
higher BOD.

g)…….deduce about operational of B.O.D………

Biochemical oxygen demand (BOD) quantity of in the water sample dissolved

oxygen that is the time needed to decolourise 0.1% methylene blue
affected by different locations / water samples.

Question 2:

MV: concentration of carbon dioxide RV: rate of photosynthesis

1. Aim/Objective:
To determine the effect of concentration of carbon dioxide
on the rate of photosynthesis

2. Statement of problem
Does the different concentration of carbon dioxide affects the rate of
photosynthesis?

3. Hypothesis
The more the concentration of carbon dioxide, the higher the
rate of photosynthesis.
4. Variable
Manipulated : concentration of carbon dioxide
Responding : number of air bubbles released per 5 minute
Constant : water temperature that is 370C // type of aquatic plant,
distance from the light source ie 5cm //power of the
bulb ie 100W.

5. Apparatus and materials

Materials: Beaker, boiling tube, light source, stopwatch, meter rule,
Retort stand.
Apparatus : aquatic plant, sodium hydrogen bicarbonate.

6. Technique
Count and record the bubbles produced by the aquatic plant
in five minutes by using a stopwatch.

7. Procedure
1. The 200 ml of water is filled in a beaker.
2. An aquatic plant is put in a test tube.
3. Pour 0.5% of sodium hydrogen bicarbonate solution in the test tube.
4. Clamp the test tube to a retort stand.
5. The test tube is place in the beaker, which the temperature of the water is fixed
at 370C
6. A light source / bulb of 100W is placed at distance of 10 cm from the beaker.
7. Then start the stopwatch, the air bubbles released are counted for five minute.
The readings is recorded in a table.
8. Steps 3 to 7 are repeated with different concentration of carbon dioxide such
as 1.0%, 1.5%, 2.0% and 2.5%..
Precaution: use the fresh aquatic plant.

(Note: Sodium hydrogen bicarbonate provides carbon dioxide gas)

Boiling Tube

Gas bubbles

beaker
Aquatic plants

Water bath (370C)

Sodium hydrogen carbonate

solution
 8. Results / Observations

Concenration of No. of bubbles Rate of

cabon dioxide ( %) released in 5 Photosynthesis
minute (No. of bubble / 5)
{ min-1}
0.5
1.0
1.5
2.0
2.5

9. Conclusion

The increases the concentration of carbon dioxide , the increases the

rate of photosynthesis. The hypothesis is accepted.

End ……………. ….