Biofloc in Aquaculture

High porosity aggregate in water body; made of

microorganism, particle, colloid, and organic matter

Biotic component (interaction of Abiotic

microorganism) component

Plankton, Particle,
Microalgae Bacteria colloid, organic
protozoa matter

(Junda, 2011)

Figure 1. Bioflok yang terapung

( www.aiyu.com, 2011)
 Biofloc Production

• Flok adalah agregat yang terbentuk oleh bakteri berfilamen,

plankton, polimer organik, dan sel-sel mikroba yang telah
mati, dan disatukan oleh polimer ekstraseluler.
• Pembentukannya dipengaruhi oleh C/N
• Perannya bagi mikroba adalah untuk meningkatkan transpor
substrat ke dalam sel dalam medium yang substratnya
terbatas.

Produksi Bioflok
 Biofloc Production

Natural resources of biofloc : BIOFLOC REACTOR

- Uncertainity floc composition Consorsium
- Unstable floc performance microalgae and
- Uncontroled water quality parameter bacteria to
optimize nutrient
cycle
CONTOH SKEMA PRODUKSI BIOFLOK

Hasil Seleksi Mikroba Pembentuk Bioflok dan Optimasi C/N

 • Produce carbohydrate, lipid ,and
Nutrition
protein as nutrition for white
supplement shrimp

Water quality • Utilize ammonia so that support

control zero water discharge system

• Produce bacteriosin against

probiotic-like pathogen
Inoculation Observation Application Treatments Analyse
• C. gracilis • Structure • Optimum • Food • Water
and and biofloc culture conversion quality
nitrifying activity period of ratio
bacteria biofloc • Bacteria
diversity
 BIOFLOC GROWTH

0,2000
0,1500

gram
0,1000
0,0500
0,0000
0 5 10 15
Day -

Suantika et al, 2011

 Application of Biofloc

 Example: application of biofloc in shrimp nursery

Nitrifying Bacteria, microalgae Chaetoceros gracilis
gracilis,, and
Probiotic Halomonas aquamarina

Suantika et al., 2011

 Role of Biofloc technology in aquaculture :
Vibrio harveyi

5,00

4,00
log jumlah sel Vibrio

3,00 A (100% pakan)

B (90% pakan : bioflok-probiotik)
C (80% pakan : bioflok-probiotik)
2,00
D (70% pakan : bioflok-probiotik)
E (60% pakan : bioflok-probiotik)
1,00

0,00
0 2 4 6 8 10
minggu ke-

Vibrio harveyi load on nursery

Gede Suantika et al, 2011

White shrimp ( post larvae 13 ) in Bacteria Microalgae
35 days cultured with biofloc:
Combination of microalgae
Thalasiossira sp and bacteria
Achromobacter liquafacreas

Achromobacter sp.  Thalassiosira sp.

 Cellulose and pectin  Diatoms
degradation  Phytoplankton
 Chitin hydrolysis  as prime producer
 Reduce accumulation of  High salinity tolerance
phosphate
 High salinity tolerance

(Suantika et al, 2007)

White shrimp ( post larvae 13 ) in 35 days cultured with :
BIOFLOC : combination of microalgae Thalasiossira sp and
bacteria Achromobacter liquafacreas

FCR SURVIVAL RATE TOTAL

BIOMASS
100 % feed 1,85 44 % 7,88 gram

10% v/v B, 50% feed 1,05 51,33 % 8,57 gram

10% v/v B, 75% feed 1,03 61,33 % 13,50 gram

10% v/v Biofloc - < 20 % 0,22 gram

(Suantika et al, 2007)

 A low or zero water exchange production “tool” used
primarily in intensive shrimp / fish production

 Utilizing pond microbial community to improve water

quality and maximize feed recycling

 Promotion of stable, heterotrophic bacteria dominated

pond environment instead of phytoplankton
dominated that is light sensitive and highly unstable

 Bacteria thrive and “floc” and flocs assimilate waste

nutrients

 In short – your pond becomes your biofilter!

Thank You