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SALIVARY BIOMARKERS AS A TOOL IN EARLY DETECTION OF ORAL

CARCINOMAS

AIM: To determine the expression pattern of miRNA’s present in the human


saliva as preliminary diagnostic tool in detection of oral cancer.

INTRODUCTION:
MicroRNAs (miRNAs) in human saliva have recently become an emerging field in
saliva research for diagnostics applications and its potential role in biological
implications. miRNAs are short noncoding RNA molecules that play important
roles in regulating a variety of cellular processes. Understanding the biological
function of miRNAs in association with diseases is important towards utilizing
miRNAs as diagnostic markers.

Salivary miRNA biomarkers have recently become an emerging field for


monitoring oral diseases and systemic diseases (Liu et al., 2012; Lau et al., 2013;
Yoshizawa et al., 2013). Early detection of oral carcinomas can improve the
survival rate of the individual drastically. Saliva has attracted increased attention
as a novel non-invasive tool for cancer diagnosis. By using the saliva sample
from the patient among other diagnostic samples make this study a versatile one.

RESEARCH METHODOLOGY :
In the present study, we examined the differential expression pattern of miRNAs
among 3 groups of subjects—including patients with habits(smoking ,alcohol,
bêtel quid chewing), second group being patients with a potentially malignant
disorder and a third group of healthy individuals with no noticeable habits.

Nanostring nCounter assay? RTqpcr? Microassay? NGS?

REFERNCES :

1. Genomewide Study of Salivary MicroRNAs for Detection of Oral Cancer


F. Momen-Heravi, A.J. Trachtenberg et al, JDR Clinical Research Supplement
vol. 93, issue 7 , suppl no. 1 .

2. Salivary MicroRNAs and Oral Cancer Detection, . Janice M. Yoshizawa and


David T. W. Wong , Janice M. Yoshizawa and David T. W. Wong, Methods Mol Biol.
2013 ; 936: 313–324. doi:10.1007/978-1-62703-083-0_24