CHINNY D.

MARCELINO 4-BSDM-B

Laboratory Exercise 2

Familiarization of Laboratory Facilities and Equipment in a Biotech Laboratory

1. Laboratories housed at the Biotechnology Unit of Southern Mindanao Agricultural Research

Center (USMARC).

Laboratory Uses and Importance

1. Plant tissue culture laboratory
2. Isozyme laboratory
3. Animal science laboratory
4. Virus-indexing laboratory
5. Molecular and biotechnology
laboratory
-used to produce clones of a plant in a method known as
micro propagation.
-To clean particular plants of viral and other infections and to
quickly multiply these plants as 'cleaned stock'
for horticulture and agriculture.
- performs electrophoretic analyses of genetically
conditioned enzyme variation by separating multiple forms
of enzymes in a solid matrix
- detection of genetic variation within and among
populations, the identification
of plant clones, hybrids and their distribution in an area, and
studies of
evolutionary dynamics in natural plant populations as well as
plant mating
systems and genetic diversity in rare or invasive species.
- housing, caretaking, genetic and microbial quality control,
anesthesia and analgesia, feeding, experimental techniques,
etc
- contribute to the reliability and reproducibility of the
results of animal experiments and to the life quality of
laboratory animals that are used for research.
-Production of virus antibody by molecular cloning and
expression technology
-Identification and characterization of plant viruses occurring
in economically important crops in, preparation and
preservation of virus antibodies, and development of
indexing techniques.
- it is already used widely in manufacturing insulin for
diabetics, and is central to a range of other biomedical
products.
-provides you with the right mixture of sound scientific
knowledge and commercial expertise to succeed in the
emerging world of biotechnology
2. Laboratory facilities that might be added to the laboratory at Biotech unit University of
Southern Mindanao Agricultural Research Center. (USMARC)

Laboratory Facilities Uses

1. Bioreactor -used for precise phototrophic cultivation of algae and
cyanobacteria. They feature a unique combination of the cultivator
and monitoring device. Light power and spectral composition as
well as the temperature and aeration gas composition can be set
with a high accuracy. The illumination unit can be removed easily
prior to sterilization and then clicked back into position following
the procedure.
2. CO2 Incubator -plate & sample drying problems are greatly reduced with high
humidity levels using this device. An anticipative logic system
controlling both temperature and CO2 levels.
3. Environmental -with programmable controller provides precise temperature and
Chamber humidity control.
4. FTIR Spectrometer The application of programmable gain amplifier, high accuracy A/D
converter and embedded computer improves the accuracy
and reliability of the whole system. It is user friendly, rich
function software enables easy, convenient and flexible operation.
Spectrum collect, spectrum conversion, spectrum collect,
spectrum conversion, spectrum processing, spectrum analyzing, and
spectrum output function etc. can be performed.
5. Particle Counter -traditional design with measuring unit, computer and oscillograph.
Advance pulse measurement technology, more than 8000 channels.
It is replaced with a pressure sensor, provides more accurate
measurement and control of degree of vacuum, and higher
automation.
6. Particle Size Analyzer -it is used to measure fog drops: Its structure can be changed
according to the requirements. A Fourrier Lens with different focal
length can be used to change the measuring range.
7. Plant Growth Chamber -are ideal for agriculture or biotechnology testing growth of plants
in an artificial climatic condition. It controls temperature, relative
humidity and illumination. The wide variety of temperatures and
light intensity patterns that are essential in plant research can now
be accurately reproduced and controlled with the updated range by
Plant Growth Chambers.
8. X-Ray Spectrometer -it can simultaneously analyze elements ranging from Na to 92U. It
is a very rapid method with determination time from 10 seconds to
1000 seconds.
9. Safety Cabinets -are designed for balance applications in the safety storage
industry. Handling of harmful and toxic compounds are done safely
thus reducing the risk of explosion. Safety Cabinets are high
performance unit that are easy to use, clean and maintain.
Particular attention is also paid to reliability and smooth running.
10. Water Purification -it is a compact system that purifies water for general laboratory
System use or as a feed source for ultrapure system. Utilizing tap water for
feed.
 3. List of available equipment in Biotech unit University of Southern Mindanao Agricultural
Research Center (USMARC).

Laboratory Facilities Uses

1 Thermal cycler - is a laboratory apparatus most commonly used to amplify
segments of DNA via the polymerase chain reaction (PCR)
2 Microcentrifuge - is a piece of laboratory equipment, driven by a motor, which
spins liquid samples at high speed. There are various types of
centrifuges, depending on the size and the sample capacity.
3 Distilling Apparatus -a set of apparatuses that uses distillation, a common operation in
many laboratories for the purpose of separating and/or purifying
components of a liquid mixture the apparatus used consists of
three major parts: distillation flask (or ‘pot’) to heat the mixture
and volatilize the components, a condenser to cool the vapors
back to liquid state, and a collection vessel.
4 Fumehood - is a type of local ventilation device that is designed to limit
exposure to hazardous or toxic fumes,vapors or dusts.
5 Pipetor/micropipettor -is a laboratory tool commonly used in chemistry, biology and
medicine to transport a measured volume of liquid, often as a
media dispenser
6 Vortex - is a simple device used commonly in laboratories to mix small
vials of liquid.
7 Water bath -is a device or a vessel for regulating the temperature of anything
subjected to heat, by surrounding the vessel containing it with
another vessel containing water which can be kept at a desired
temperature.
8 Horizontal gel -also called Agarose gel electrophoresis because it uses Agarose
electrophoresis gel. It maintains a uniform electric field across the gel, provide
apparatus cooling to prevent thermal artifacts, and allow access to the gel for
sample loading and monitoring the run.
9 Gel documentation - is equipment widely used in molecular biology laboratories for
system the imaging and documentation of nucleic acid and protein
suspended within polyacrylamide or agarose gels. These gels are
typically stained with ethidium bromide or other fluorophores such
as SYBR Green
10 Polyacrylamide gel - a gel electrophoresis device that uses acrylamide gel that is use in
electrophoresis a separation method frequently
used to analyze DNA fragments generated by restriction enzymes,
and it
is a convenient analytical method for determining the size of DNA
molecules
in the range of 500 to 30,000 base pairs. It can also be used to
separate other charged biomolecules such as dyes, RNA and
proteins.
11 Microwave oven -is a device that heats food by bombarding it with electromagnetic
radiation in the spectrum causing polarized molecules in the food
to rotate and build up thermal energy in a process known as
dielectric heating.
12 Stirrer/hot plate -is a portable self-contained tabletop small appliance that features
one, two or more gas burners or electric heating elements. A hot
plate can be used as a stand-alone appliance, but is often used as a
substitute for one of the burners from an oven range or the cook
top of a stove.
13 Electronic digital -is a weighing scale that is used for measuring substances. It can be
balance/Analytical found in place where large quantities of food are prepared like
balance hotel kitchens and institutional kitchens. People also use them at
home. They are easier to use and they provide more accurate
readings.
14 Biofreezer -is a kind of freezer that user a topical over-the-counter gel for
pain relief consisting of menthol with and other ingredients.
15 Ice maker - may refer to either a consumer device for making ice, found
inside a home freezer; a stand-alone appliance for making ice, or
an industrial machine for making ice on a large scale.
16 Autoclave/sterilizer - is a device used to sterilize equipment and supplies by subjecting
them to high pressure saturated steam at 121 °C for around 15–20
minutes depending on the size of the load and the contents.
17 Binder -It is suitable for all sensitive incubation applications and ensures
optimal cell growth.
18 Dual action shaker -is a device that have Orbital or reciprocating shaking action over a wide
speed range (10 to 300 rpm) offering an ideal solution for your mixing needs
19 Beakers -is a simple container for stirring, mixing and heating liquids
commonly used in many laboratories.
20 Cylinders/Graduated -is a piece of laboratory equipment used to measure the volume of
cylinders a liquid. Graduated cylinders are generally more accurate and
precise than laboratory flasks and beakers.
21 Erlenmeyer flask -The tapered sides and narrow neck of this flask allow the contents
of the flask to be mixed by swirling, without risk of spillage, making
them suitable for titrations. Such features similarly make the flask
suitable for boiling liquids. Hot vapors condense on the upper
section of the Erlenmeyer flask, reducing solvent loss. Erlenmeyer
flasks' narrow necks can also support filter funnels.
22 Syringe -is a simple pump consisting of a plunger that fits tightly in a tube.
The plunger can be pulled and pushed along inside a cylindrical
tube (called a barrel), allowing the syringe to take in and expel
a liquid or gas through an orifice at the open end of the tube.
23 Thermometers -is a device that measures temperature or temperature gradient
using a variety of different principles.