LABORATORIO DE ANÁLISIS INSTRUMENTAL

PROGRAMA DE QUÍMICA
UNIVERSIDAD DEL QUINDÍO

Sample Information
Analyzed by : Admin
Analyzed : 5/3/2019 5:39:33 PM
Sample Type : Unknown
Level # :1
Sample Name : Piperina-SENA
Sample ID : Piperina-SENA
IS Amount : [1]=1
Sample Amount :1
Dilution Factor :1
Vial # :2
Injection Volume : 1.00
Data File : C:\GCMSsolution\Data\DOCENCIA\FITO II\Piperina pimienta 02-05-2019\Metodo 5\Piperina-SENA.q
Org Data File : C:\GCMSsolution\Data\DOCENCIA\FITO II\Piperina pimienta 02-05-2019\Metodo 5\Piperina-SENA.q
Method File : C:\GCMSsolution\Data\DIPLOMADOS\DIPLOMADO FORENSE\THC calibracion split 20 - 30 min.q
Org Method File : C:\GCMSsolution\Data\DIPLOMADOS\DIPLOMADO FORENSE\THC calibracion split 20 - 30 min.q
Report File : C:\GCMSsolution\Data\REPORTES\CATALISIS\rEPORTE_MANUEL.qgr
Tuning File : C:\GCMSsolution\System\Tune1\24-01-19 tuning.qgt
Modified by : Admin
Modified : 5/6/2019 4:31:04 PM
Chromatogram Piperina-SENA C:\GCMSsolution\Data\DOCENCIA\FITO II\Piperina pimienta 02-05-2019\Metodo 5\Piperina-SENA.qgd
TIC
30,000,000

2
1

2.0 10.0 20.0 25.0

min
Peak Report TIC
Peak# R.Time Name Area Area% Height Height% A/H
1 2.140 Caryophyllene 919943 0.83 725903 6.96 1.27
2 16.605 Piperine 110156977 99.17 9698793 93.04 11.36
111076920 100.00 10424696 100.00
 Spectrum

Peak#:1 R.Time:2.140(Scan#:9)
MassPeaks:247
RawMode:Averaged 2.130-2.150(7-11)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
110
41
100

90

80

70
69
60

50 79 93

40
105
55 133
30

20 119
10 40 148
175 204 224242 264 287 315 334 356 388405 429 452 475 495
30 70 110 150 190 230 270 310 350 390 430 470
m/z
Peak#:2 R.Time:16.605(Scan#:2902)
MassPeaks:362
RawMode:Averaged 16.595-16.615(2900-2904)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
110
115
100

90

80
201
70

60
285
50
84 173
40 143
30

20
41
63 100 137
10 42 159
77
40 187 214 242 268 295313 341 370 389407 429 456 481
30 70 110 150 190 230 270 310 350 390 430 470
m/z
Library
<< Target >>
Line#:1 R.Time:2.140(Scan#:9) MassPeaks:247
RawMode:Averaged 2.130-2.150(7-11) BasePeak:41.00(68634)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 41
69
79 93
50 55 105
119
133
40 148 161 175 189 204 224 235 264 276 287 315 334 348 371 388 405 429 441 452 475 489

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:1 Entry:18022 Library:NIST11s.lib
SI:91 Formula:C15H24 CAS:118-65-0 MolWeight:204 RetIndex:1494
CompName:Bicyclo[7.2.0]undec-4-ene, 4,11,11-trimethyl-8-methylene-,[1R-(1R*,4Z,9S*)]- $$ Isocar
100 41 69 93
70 79 133
55 107
40 27 120
148 161
175 189 204

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:2 Entry:18028 Library:NIST11s.lib
SI:90 Formula:C15H24 CAS:489-39-4 MolWeight:204 RetIndex:1386
CompName:Aromandendrene $$ 1H-Cycloprop[e]azulene, decahydro-1,1,7-trimethyl-4-methylene-, [
100 41
70 79
91
105
40 27 55 67 119 133
147
161
204
175 189

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:3 Entry:18026 Library:NIST11s.lib
SI:88 Formula:C15H24 CAS:495-61-4 MolWeight:204 RetIndex:1500
CompName:.beta.-Bisabolene $$ Cyclohexene, 1-methyl-4-(5-methyl-1-methylene-4-hexenyl)-, (S)- $
100 41
69
70 93

40 54
79
107 119
28 133 147 161 176 189 204

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:4 Entry:18025 Library:NIST11s.lib
SI:87 Formula:C15H24 CAS:28973-97-9 MolWeight:204 RetIndex:1440
CompName:cis-.beta.-Farnesene $$ 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- $$ (Z)-.be
100 41
69
70
40 53 79
93
133
28 107 120 148 161 175 189 204

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:5 Entry:18054 Library:NIST11s.lib
SI:87 Formula:C15H24 CAS:495-61-4 MolWeight:204 RetIndex:1500
CompName:.beta.-Bisabolene $$ Cyclohexene, 1-methyl-4-(5-methyl-1-methylene-4-hexenyl)-, (S)- $
100 41 69
93
70
40 55
79 109 119
204
27 135 147 161
176 189

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
<< Target >>
Line#:2 R.Time:16.605(Scan#:2902) MassPeaks:362
RawMode:Averaged 16.595-16.615(2900-2904) BasePeak:115.00(1137491)
BG Mode:Calc. from Peak Group 1 - Event 1 Scan
100 115 201
285
50 84 143 173
63 77 100 137 159
40 187 214 229 242 256 268 295 313 325 341 356 370 389 405 429 440 456 467 481491

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:1 Entry:25223 Library:NIST11s.lib
SI:93 Formula:C17H19NO3 CAS:94-62-2 MolWeight:285 RetIndex:2399
CompName:Piperine $$ Piperidine, 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-, (E,E)- $$ Pi
100 115 201 O

60 173 285
O N

84 O
20 39 55 63 77
100 137
159 187 212 228 242 254 268

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:2 Entry:25222 Library:NIST11s.lib
SI:91 Formula:C17H19NO3 CAS:94-62-2 MolWeight:285 RetIndex:2399
CompName:Piperine $$ Piperidine, 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-, (E,E)- $$ Pi
100 115 201 O
285
60 173
O N

143 O
20 39 63 77
89
100 137 159 187 214 228 242 256 268

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:3 Entry:25225 Library:NIST11s.lib
SI:90 Formula:C17H19NO3 CAS:94-62-2 MolWeight:285 RetIndex:2399
CompName:Piperine $$ Piperidine, 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-, (E,E)- $$ Pi
100 201 O
115 285
60 173
O N

84 O
20 42 55 63 77 100 137 159 187 214 229 242 256 268

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:4 Entry:25220 Library:NIST11s.lib
SI:86 Formula:C17H19NO3 CAS:94-62-2 MolWeight:285 RetIndex:2399
CompName:Piperine $$ Piperidine, 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-, (E,E)- $$ Pi
100 115
201
O

60 173
O N

84 O
137 285
20 40 55 69 81 100 159

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
Hit#:5 Entry:25224 Library:NIST11s.lib
SI:82 Formula:C17H19NO3 CAS:94-62-2 MolWeight:285 RetIndex:2399
CompName:Piperine $$ Piperidine, 1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]-, (E,E)- $$ Pi
100 115 201 O
285
60 O N

O
20 26 41 63 77 84 100 137 159
173
187 229 256 268

20 50 80 110 140 170 200 230 260 290 320 350 380 410 440 470
 Method

[Comment]

===== Analytical Line 1 =====

[AOC-20i+s]
# of Rinses with Presolvent :2
# of Rinses with Solvent(post) :2
# of Rinses with Sample :3
Plunger Speed(Suction) :High
Viscosity Comp. Time :0.2 sec
Plunger Speed(Injection) :High
Syringe Insertion Speed :High
Injection Mode :Normal
Pumping Times :5
Inj. Port Dwell Time :0.3 sec
Terminal Air Gap :No
Plunger Washing Speed :High
Washing Volume :8uL
Syringe Suction Position :0.0 mm
Syringe Injection Position :0.0 mm
Solvent Selection :All A,B,C

[GC-2010]
Column Oven Temp. :200.0 °C
Injection Temp. :250.00 °C
Injection Mode :Split
Flow Control Mode :Linear Velocity
Pressure :111.5 kPa
Total Flow :21.5 mL/min
Column Flow :1.00 mL/min
Linear Velocity :38.7 cm/sec
Purge Flow :0.5 mL/min
Split Ratio :20.0
High Pressure Injection :OFF
Carrier Gas Saver :OFF
Splitter Hold :OFF
Oven Temp. Program
Rate Temperature(°C) Hold Time(min)
- 200.0 2.00
10.00 260.0 15.00
10.00 300.0 3.00

< Ready Check Heat Unit >

Column Oven : Yes
SPL1 : Yes
MS : Yes
< Ready Check Detector(FTD) >
< Ready Check Baseline Drift >
< Ready Check Injection Flow >
SPL1 Carrier : Yes
SPL1 Purge : Yes
< Ready Check APC Flow >
< Ready Check Detector APC Flow >
External Wait :No
Equilibrium Time :1.0 min
[GC Program]

[GCMS-QP2010 Ultra]
IonSourceTemp :250.00 °C
Interface Temp. :280.00 °C
Solvent Cut Time :2.00 min
Detector Gain Mode :Relative
Detector Gain :1.18 kV +0.00 kV
Threshold :0

[MS Table]
--Group 1 - Event 1--
Start Time :2.10min
End Time :30.00min
ACQ Mode :Scan
Event Time :0.30sec
Scan Speed :1666
Start m/z :40.00
End m/z :500.00

Sample Inlet Unit :GC

[MS Program]
Use MS Program :OFF