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Introduction:
The microorganism that I have selected is Lactobacillus acidophilus. "Lacto" means milk and
"bacillus" means rod like. Acidophilus means it is acid loving. It is a member of the Kingdom
Eubacteria.
General characteristics:
It is a gram positive bacteria
L. acidophilus produces the acids that make food taste sour
It is rod shaped coccobacilli
It is homofermentative specie.
It ferments sugar into lactic acid
It is non-spore-forming bacteria.
It is facultative anaerobic or microaerophilic,
It is very acid-resistant and tolerates bile quite effectively
It is well known that Lactobacillus acidophilus is catalase negative
Cultural characteristics:
Part of the normal flora of mouth, intestinal tract and vagina of humans and other warm-blooded
animals. Also found in dairy meat, fish, pickled and fermented products
Growth Requirements:
It grows readily between pH 6.5 to 8.
It has an optimum growth temperature of around 37 °C (99 °F)
They can grow in the absence of oxygen
RECOMMENDED MEDIA
For culture: Rich, complex media.
For selective isolation: Universal Beer Agar, MRS Agar and Broth.
For maintenance: Skim Milk Media may be used for storage at -70 degrees C.
Lyophilization may be used for preservation.
INCUBATION
Temperature: 30-35 degrees C.
Time: 24-72hr
Strains
Laboratory: NCFM, 4962, CNRZ216, CNRZ218
Human: HA1, HA2, HA3, HM2, HM6
Pig: PA3, PA12, PA19, P18, P47
Chicken: C1, C2, C3, C7, C11
Isolating Methods:
Sterilize all the things except sample
Prepare the media (MRS agar)
Pour the media into petri plates
Curd is taken in sterilized flask. Under aseptic conditions curd was serially diluted from
10-1 to 10-14 from this 14 dilutions 10-5 10-7 10-9 are selected.
For this 3 tubes spread plate technique farther with streak plate technique is done on
MRS medium.
They are incubated in incubator at 37°C for 24 hours.
After the period of incubation 3 isolated colonies were grown
Biochemical tests result:
Many test reactions are weak and dependent on the composition of the media and exact cultural
conditions
TEST OBSERVATIONS
Arabinose No fermentation
Sorbitol No fermentation
Colony morphology:
MICROSCOPIC APPEARANCE
Gram Stain: Positive.
Capsules: None.
Spores: None.
Colony appearance:
Typically, colonies on agar media are usually 2-5 micrometers, convex, entire, opaque, and
without pigment.
Lactobacillus acidophilus (ATCC ® 4356) colonies growing on Lactobacilli MRS Agar (Cat. no. G117). Incubated in CO 2 for 72 hours at
35ºC.
References:
https://microbewiki.kenyon.edu/index.php/Lactobacillus_acidophilus
https://www.ijppsjournal.com/Vol6Issue1/7982.pdf
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/LactobacilliMRSAgar.html
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/Lactobacillus.htm
http://bacmap.wishartlab.com/organisms/1292