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If you receive a plasmid from a collaborator in the mail, you will usually get only a few microlitres.
It's better not to amplify the plasmid in protein expression strains, since they are not always well
suited to plasmid extraction and purification. To amplify, transform into E. coli DH5α , pick a
single colony and do a mini-plasmid preparation as usual. Check with the donator or the
appropriate reference what is the antibiotic selection for the plasmid. It's usually ampicillin, but
can also kanamycin, tetracycline or chloramphenicol. Second, find out the copy number of the
plasmid (low or high). This will determine how much culture is grown for plasmid extraction.
*Antibiotics:
KanR: 50 µ g/ml kanamycin
AmpR: 100 µ g/ml carbenicillin/ampicillin
TetR: 12.5 µ g/ml tetracycline
CamR: 25 to 34 µ g/ml chloramphenicol