Salient Features:-

The unit will produce fungal cellulase for detergent, food, paper, textile and fruit processing industries in
the first phase. Subsequently, the unit will be diversified for production of Pectinase, Protease, Amylase
etc with same basic infrastructure after necessary augmentation.

Potential use of Cellulase

Detergents
Cellulase-based detergents; superior cleaning action without damaging fibers; improved color brightness
and dirt removal; remove of rough protuberances in cotton fabrics; antiredeposition of ink particles.

Fermentation
Improved malting and mashing; improved pressing and color extraction of grapes; improved aroma of
wines; improved primary fermentation and quality of beer; improved viscosity and filterability of wort;
improved must clarification in wine production; improved filtration rate and wine stability

Food
Release of the antioxidants from fruit and vegetable pomace; improvement of yields in starch and protein
extraction; improved maceration, pressing, and color extraction of fruits and vegetables; clarification of
fruit juices; improved texture and quality of bakery products; improved viscosity fruit purees; improved
texture, flavor, aroma, and volatile properties of fruits and vegetables; controlled bitterness of citrus
fruits.

Pulp and Paper

Coadditive in pulp bleaching; biomechanical pulping; improved draining; enzymatic deinking; reduced
energy requirement; reduced chlorine requirement; improved fiber brightness, strength properties, and
pulp freeness and cleanliness; improved drainage in paper mills; production of biodegradable cardboard,
paper towels, and sanitary paper.

Textile
Biostoning of jeans; biopolishing of textile fibers; improved fabrics quality; improved absorbance property
of fibers; softening of garments; improved stability of cellulosic fabrics; removal of excess dye from
fabrics; restoration of colour brightness.

Agriculture Plant
pathogen and disease control; generation of plant and fungal protoplasts; enhanced seed germination
and improved root system; enhanced plant growth and flowering; improved soil quality; reduced
dependence on mineral fertilizers.

Bioconversion
Conversion of cellulosic materials to ethanol, other solvents, organic acids and single cell protein, and
lipids; production of energy-rich animal feed; improved nutritional quality of animal feed; improved
ruminant performance; improved feed digestion and absorption; preservation of high quality fodder.

Others
Improved carotenoids extraction; improved oxidation and colour stability of carotenoids; improved olive
oil extraction; improved malaxation of olive paste; improved quality of olive oil; reduced risk of biomass
waste; production of hybrid molecules; production of designer cellulosomes.
INFRASTRUCTURAL AND OTHER INVESTMENTS

A. Company formation and building:

Particulars Rs. In lakh

1. Building construction effective area- 2000 sq. Ft. With false ceiling 30.00
and vinyl flooring @ Rs. 1500 per sq. Ft.

2. Separate sterile glass room 350 sq. Ft 2.00

3. Furniture and computers 2.00

4. Licence fees(Trade, fire, pollution etc) 0.20

5. Misc. Expenses 0.50

_________________

34.70

B. R&D machineries
(For research and quality control) Rs. In lakh(approx)

1. Autoclave (41”x 41”) 0.20

2. Phase contrast microscope 2.00

3. UV-VIS spectrophotometer 1.00

4. Laminar air flow 1.00

5. Desk top centrifuge(18”) 0.30

6. Digital weighing machine 30 kg 0.05

7. Bacteriological incubator 340L 0.30

8. -20 degree freezer 0.20

9. 320 L double door normal refrigerator 0.15

10. Hot air oven (24”x 24”x 36”) 0.30

11. Digital pH meter 0.05

12. Distillation plant (10 liters per hour capacity) 0.10

13. Vortex 0.02

14. Water bath 0.08

15. Magnetic starrer with hot plate 0.07

16. Vacuum desiccators 0.10

17. Mixer grinder 0.03

18. Filter press unit(3 different filter size; 1, .5, .25 micron) 0.15

19. Shaking incubator( double decker) 0.30

20. Micro pipettes 0.10

C. Consumable chemicals and glassware 4.00

---------------------
10.70

D. Human resource requirements:

Per month salary(in lakh)

1. Advisory and technical guidance 0.40

2. 1 R&D Scientist (M.Sc Microbiology / Biotechnology) 0.15

3. 1 Production Assistant (B.Sc Biology/ Microbiology) 0.08

4. 1 Quality Control Scientist (M.Sc Chemistry/ Biochemistry) 0.10

5. 2 Labour 0.04x2= 0.08

6. 1 accountant cum office manager 0.08

_______________
0.89

** Other expenses (technology, organism etc ) 1.00