PMID- 31369634

OWN - NLM
STAT- In-Data-Review
LR - 20190801
IS - 1932-6203 (Electronic)
IS - 1932-6203 (Linking)
VI - 14
IP - 8
DP - 2019
TI - IA-Lab: A MATLAB framework for efficient microscopy image analysis
development,
applied to quantifying intracellular transport of internalized peptide-drug
conjugate.
PG - e0220627
LID - 10.1371/journal.pone.0220627 [doi]
AB - This work presents a MATLAB-based software package for high-throughput
microscopy
image analysis development, making such development more accessible for a
large
user community. The toolbox provides a GUI and a number of analysis
workflows,
and can serve as a general framework designed to allow for easy extension.
For a
new application, only a minor part of the object-oriented code needs to be
replaced by new components, making development efficient. This makes it
possible
to quickly develop solutions for analysis not available in existing tools. We
show its use in making a tool for quantifying intracellular transport of
internalized peptide-drug conjugates. The code is freely available as open
source
on GitHub (https://github.com/amcorrigan/ia-lab).
FAU - Corrigan, Adam M
AU - Corrigan AM
AUID- ORCID: http://orcid.org/0000-0001-5142-6579
AD - Discovery Sciences, R&D, AstraZeneca, Cambridge, United Kingdom.
FAU - Karlsson, Johan
AU - Karlsson J
AD - Discovery Sciences, R&D, AstraZeneca, Gothenburg, Sweden.
FAU - Wildenhain, Jan
AU - Wildenhain J
AD - Discovery Sciences, R&D, AstraZeneca, Cambridge, United Kingdom.
FAU - Knerr, Laurent
AU - Knerr L
AD - Research and Early Development, Cardiovascular, Renal and Metabolism,
BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
FAU - Olwegard-Halvarsson, Maria
AU - Olwegard-Halvarsson M
AD - Research and Early Development, Cardiovascular, Renal and Metabolism,
BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
FAU - Karlsson, Maria
AU - Karlsson M
AD - Research and Early Development, Respiratory, Inflammation and Autoimmune,
BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
FAU - Lunse, Svenja
AU - Lunse S
AD - Discovery Sciences, R&D, AstraZeneca, Gothenburg, Sweden.
FAU - Wang, Yinhai
AU - Wang Y
AD - Discovery Sciences, R&D, AstraZeneca, Cambridge, United Kingdom.
LA - eng
PT - Journal Article
DEP - 20190801
PL - United States
TA - PLoS One
JT - PloS one
JID - 101285081
SB - IM
COIS- The funding for this research was provided by AstraZeneca. All authors are or
were employees of AstraZeneca. There are no patents, products in development
or
marketed products to declare. This does not alter the authors' adherence to
all
the PLOS ONE policies on sharing data and materials, as detailed online in
the
guide for authors.
CRDT- 2019/08/02 06:00
PHST- 2018/12/20 00:00 [received]
PHST- 2019/07/19 00:00 [accepted]
PHST- 2019/08/02 06:00 [entrez]
AID - 10.1371/journal.pone.0220627 [doi]
AID - PONE-D-18-36390 [pii]
PST - epublish
SO - PLoS One. 2019 Aug 1;14(8):e0220627. doi: 10.1371/journal.pone.0220627.
eCollection 2019.