Vous êtes sur la page 1sur 7

67th Conference on

Mass Spectrometry and Allied Topics


Sponsored by
June 2 - 6, 2019
American Society for Atlanta, Georgia
Mass Spectrometry

SHORT COURSES June 1 - 2

Susan Richardson
Vice President for Programs

Important Dates
January 15
Deadline to apply for
The conference and short courses will be held in the Georgia World Congress Center. Corporate
undergraduate or graduate
hospitality suites will be at the Omni CNN Center Hotel.
student conference
travel awards
The Georgia World Congress Center is surrounded by a range of hotels and restaurants in the heart of
February 1 downtown Atlanta.
Deadline for abstract Corporate Membership and Exhibits. The participation of ASMS Corporate Members is an integral part of
submission the conference. A Corporate Member exhibit booth or hospitality suite provides access to over 6,000 scientists
April 30 attending one of the most dynamic conferences in the world. Information is online at www.asms.org.
Deadline for advance Social Events.
conference registration and • Sunday: Welcome Reception in the poster/exhibit hall immediately follows the opening plenary session.
short course registration A buffet of heavy hors d’oeuvres, beer and soft beverages are free to all conference registrants.
• Monday - Thursday: Poster session coffee break and afternoon pick-me-up snacks in the poster/exhibit hall.
April 30 • Monday - Wednesday: Light snacks, beer and beverages bridge the transition from plenary session to
Deadline for hotel reservations workshops as you meet-up to discuss the science of the day.
at the conference rates • Thursday: Closing Event at the Georgia Aquarium. An incredible opportunity to visit one of the world’s
largest aquariums. A dinner buffet and full aquarium access is included. This event is a value of over $150.
Your cost is $40 per person. Purchase tickets online via conference registration. Tickets can be added on to
your existing conference registration at any time.
Travel and Accommodations.
Hartsfield-Jackson Atlanta International Airport (ATL) is one of the busiest in the world with daily non-
stop flights to hundreds of cities. Go to asms.org for details on airport public transportation (MARTA) and ride
share options.
Hotel reservations are managed online. Visit asms.org for hotels, rates and map.

www.asms.org
Short Courses
Go to www.asms.org to view a description of each course.
Short courses have limited enrollments. The deadline for registration is April 30. There is no on-site registration for short courses.
TWO-DAY SHORT COURSES 07 LC-MS: Advanced Techniques and Applications 14 Case Studies in Quantitative Proteomics
Saturday and Sunday, June 1 - 2, 2019 Instructor: Jack Henion (Advion, Inc.) Instructors: Meena Choi (Northeastern University), Jake Jaffe (The Broad
08 LC-MS: Practical Maintenance and Troubleshooting Institute), Brendan MacLean (University of Washington), Olga Vitek
01 Bioinformatics for Protein Identification
Instructors: Susan Abbatiello (Northeastern University), Thomas Blau (Ion (Northeastern University)
Instructors: Nuno Bandeira (University of California San Diego),
Alexey Nesvizhskii (University of Michigan Health System), David Tabb Technology Support, Inc), Will Thompson (Duke University) 15 Untargeted Metabolomics: From Basic Methods to Advanced
(Stellenbosch University) 09 Native Mass Spectrometry Workflows and Isotope Labeling
Instructors: Vicki Wysocki (The Ohio State University), Sophie Harvey Instructor: Gary Patti (Washington University School of Medicine)
02 Clinical Diagnostics: Innovation, Validation, Implementation and
Operation by Mass Spectrometry (The Ohio State University), David Russell (Texas A&M University), Arthur
Instructors: Matthew Crawford, Brian Rappold and Chris Shuford (LabCorp) Laganowski (Texas A&M University) ONE-DAY SHORT COURSES
03 DMPK: Experimentation and Data Interpretation 10 Peptides and Proteins in Mass Spectrometry Sunday June 2, 2019
Instructors: Mark Hayward (ITSP Solutions and Active Ingredient Tech- Instructors: Arthur Moseley (Duke University), Douglas Sheeley (NIH), Eric 17 Introduction to GLP Regulations and Bioanalytical Method
nologies), Mike S. Lee (Milestone Development Services), Naidong Weng Soderblom (Duke University) Validation by LC-MS/MS (Sunday only)
(Janssen Research and Development), Mingshe Zhu (Bristol-Myers Squibb) 11 Protein Structural Analysis by Mass Spectrometry: Hydrogen Instructor: Perry Wang (LC-MS Technical Expert)
Exchange and Covalent Labeling 18 Ion Mobility Mass Spectrometry: An Introduction to
04 Glycans and Glycoproteins in Mass Spectrometry
Instructors: Mark Chance (Case Western Reserve), Roxana Iacob Instrumentation, Applications, and Data Analysis (Sunday only)
Instructors: Jon Amster (University of Georgia), Carlito Lebrilla (University
(Northeastern University), Janna Kiselar (Case Western Reserve), Thomas Instructors: Erin Baker (North Carolina State University), Brian H. Clowers
of California, Davis), Ron Orlando (University of Georgia), Joe Zaia (Boston
Wales (Northeastern University) (Washington State University), John A. McLean (Vanderbilt University)
University)
12 Protein Therapeutics: Practical Characterization and 19 Advanced Metabolomics: Bioinformatics, Activity &
05 High Resolution Mass Spectrometry for Qualitative and
Quantitation by Mass Spectrometry Systems Biology
Quantitative Analysis: An Introduction
Instructors: Guodong Chen and Li Tao (Bristol-Myers Squibb) Instructors: Gary Siuzdak, H. Paul Benton, Xavi Dominguez and Erica
Instructors: Ragu Ramanathan, Matthew Blatnik, Cong Wei (Pfizer)
13 Quantitative Mass Spectrometry Majumder (The Scripps Research Institute)
06 Understanding, Optimizing and Applying LC/MS/MS Techniques
Instructors: Dwight E. Matthews (University of Vermont) and Robert Bethem
using Electrospray, APCI and APPI to Develop Successful Methods
(Consultant)
Instructor: Robert D. Voyksner (LCMS Limited)

2019 PROGRAM OVERVIEW


SATURDAY JUNE 1 TUESDAY JUNE 4
9:00 am - 5:00 pm Short Courses 8:30 - 10:30 am Oral Sessions
2:00 - 5:00 pm Conference Registration • TOA Informatics: Innovations
• TOB Fundamentals: Photoionization and Photodissociation
SUNDAY JUNE 2 • TOC Native MS in Structural Biology
9:00 am - 5:00 pm Short Courses • TOD Imaging: Pharmaceuticals, Metabolites, and Lipids
10:00 am - 8:00 pm Conference Registration • TOE Environmental: Emerging Contaminants (In Honor of Ron Hites)
5:00 - 6:30 pm Tutorial Lectures • TOF Protein-Ligand Interactions
Targeted Imaging Data Independent Acquisition • TOG MS in the QC Lab
Enrico Davoli Birgit Schilling • TOH Nucleic Acids and Oligonucleotides
(Mario Negri Institute) (Buck Institute) 10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break
2:30 - 4:30 pm Oral Sessions
Native Mass Spectrometry Lipidomics
• TOA Informatics: Data-Independent Acquisition
Michal Sharon Stephen Blanksby
• TOB GCxMS, GC/GC/MS, GC-MS/MS, and GC/HRMS
(Weizmann Institute) (Queensland University of Technology)
• TOC Top Down Protein Analysis
Gavin Reid
• TOD Drug Target Identification by MS
(University of Melbourne)
• TOE Food Safety & Chemistry: Innovations
6:45 - 7:45 pm Opening Session: Plenary Lecture • TOF Cancer Research
• TOG Instrumentation: Innovative Separations Approaches Coupled to MS
Mark Z. Jacobson (Stanford University) • TOH Energy, Petroleum, and Biofuels: Instrumentation and Applications
Transitioning the World Energy for All Purposes
to Stable Electricity Powered by 100% Wind, 4:45 - 5:30 pm Plenary Session: Biemann Medal
Water, and Sunlight 5:45 - 7:00 pm Workshops
After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel
7:45 - 9:30 pm Welcome Reception in the Poster/Exhibit Hall
WEDNESDAY JUNE 5
MONDAY JUNE 3 8:30 - 10:30 am Oral Sessions
8:30 - 10:30 am Oral Sessions • WOA Metabolomics: New Technologies and Applications
• MOA Cannabis Testing • WOB Carbohydrates
• MOB Glycopeptides and Glycoproteins • WOC Fundamentals for Everyone: Peptides and Proteins
• MOC Membrane Protein MS • WOD Microdosing and Microsampling: Analytical Challenges
• MOD Imaging: Instrumentation & Method Development • WOE Environmental: Innovative Approaches and Instrumentation
• MOE Lipidomics: Targeted and Untargeted • WOF Ion Mobility: New Developments & Applications
• MOF Fundamentals: Ion Mobility and MS (In Memory of Al Yergey) • WOG Fundamentals for Everyone: Structural Elucidation
• MOG Instrumentation: Portable and Transportable Mass Spectrometers • WOH Synthetic Polymers
• MOH Biomarkers: Qualitative Analysis
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break 2:30 - 4:30 pm Oral Sessions
2:30 - 4:30 pm Oral Sessions • WOA Metabolomics: Untargeted Profiling
• MOA Informatics: Multiomics Integration and Applications • WOB Hydrogen-Deuterium Exchange MS: Innovations
• MOB Homeland Security: Chemical/Biological Defense • WOC Forensics: Innovations and Applications
• MOC Food Safety & Chemistry: Foodomics, Allergens, Bacteria, Foods, and Supplements • WOD Endogenous Protein Biomarkers in Drug Discovery and Development: Quantitative
• MOD Therapeutic Proteins, Antibodies, and Antibody/Drug Conjugates Analysis
• MOE Lipidomics: New MS Technologies and Applications • WOE Clinical Analysis: MS in the Operating Room
• MOF Biomarkers: Quantitative Analysis • WOF Ion Mobility: Small Molecules, Pharmaceuticals, and DMPK
• MOG Instrumentation: New Developments in Ionization and Sampling • WOG Instrumentation: Ambient Ionization & Applications
• MOH Art, Archaeology, and Paleontology • WOH Fundamentals: DDA and DIA LC-MS
4:45 - 5:30 pm Plenary Session: Award for a Distinguished Contribution in Mass Spectrometry 4:45 - 5:30 pm ASMS Meeting
5:45 - 7:00 pm Workshops 5:45 - 7:00 pm Workshops
After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel After 8:00 pm Corporate Hospitality Suites, Omni CNN Center Hotel
Program Overview
THURSDAY JUNE 6 • ThOE Plant “omics”
8:30 - 10:30 am Oral Sessions • ThOF Ion Mobility: Structure
• ThOA Informatics: Metabolomics • ThOG Instrumentation: Innovations in Mass Analyzers
• ThOB Fundamentals: Ion Spectroscopy • ThOH Fundamentals: Ion Activation and Dissociation
• ThOC Posttranslational Modifications: Qualitative and Quantitative
4:45 - 5:30 pm Closing Session: Plenary Lecture
• ThOD Drug Discovery and Development: Quantitative Analysis
• ThOE Supramolecular and Macromolecular Complexes Lilly D’Angelo (Global Food & Beverage Technology Associates)
• ThOF Clinical Analysis using MS Chemistry of Food and Soft Drinks
• ThOG Stable Isotope Labeling in MS: Applications

• ThOH Exposomics, Toxicology, and Human Health
10:30 am - 2:30 pm Poster Session, Exhibits, Lunch Break 6:30 - 9:30 pm Closing Event, Georgia Aquarium (advance ticket purchase required)
2:30 - 4:30 pm Oral Sessions
• ThOA Informatics: Peptide and Protein Identification, Proteomics
• ThOB Microorganisms and the Microbiome
• ThOC Quantitative Proteomics in Systems Biology
• ThOD Covalent Labeling and Chemical Crosslinking

ASMS CONFERENCE & SHORT COURSE REGISTRATION, Deadline: April 30, 2019
Register online: www.asms.org The deadline for advance registration is April 30. After April 30, fees increase by $50. Short Course registration closes on April 30.
Cancellations. To cover the cost of handling and processing, $40 is deducted from the total refund for cancellations. Conference registration cancellation must be received in writing on or before May 15, 2019. Short Course cancellation
must be received by May 15.
Name: I am an UNDERGRADUATE student.
Company/Institution:
Address:
Address:
Phone: Email:
Advance Conference Registration
 ASMS Member, $200*  ASMS Student Member, $90**  Non-member, $400
 Student Non-member, $160**  Emeritus Member, No Charge Enter registration amount $ ___________

Check ONE course for which you wish to register


ü TWO-DAY SHORT COURSES (Saturday and Sunday) ASMS member* Non-Member Student**
01 Bioinformatics for Protein Identification $400 $700 $150
02 Clinical Diagnostics by MS, Innovation, Validation, Implementation, and Operation by MS $400 $700 $150
03 DMPK: Experimentation and Data Interpretation, includes cost of textbook. $506 $806 $256
04 Glycans and Glycoproteins in Mass Spectrometry $400 $700 $150
05 High Resolution MS for Qualitative and Quantitative Analysis: An Introduction $400 $700 $150
06 Understanding, Optimizing and Applying LC/MS/MS Techniques using Electrospray, APCI and APPI to Develop Successful Methods $400 $700 $150
07 LC-MS: Advanced Techniques and Applications $400 $700 $150
08 LC-MS: Practical Maintenance and Troubleshooting $400 $700 $150
09 Native Mass Spectrometry $400 $700 $150
10 Peptides and Proteins in Mass Spectrometry $400 $700 $150
11 Protein Structural Analysis by Mass Spectrometry, includes cost of textbook $477 $777 $227
12 Protein Therapeutics: Practical Characterization and Quantitation $400 $700 $150
13 Quantitative Mass Spectrometry $400 $700 $150
14 Case Studies in Quantitative Proteomics $400 $700 $150
15 Untargeted Metabolomics: From Basic Methods to Advanced Workflows and Isotope Labeling $400 $700 $150

Check one or two courses for which you wish to register


ü ONE-DAY SHORT COURSES Member* Non-Member Student**
17 Intro to GLP Regulations and Bioanalytical Method Validation by LC-MS/MS (Sunday only) $300 $500 $75
18 Ion Mobility MS: Instrumentation, Applications, & Data Analysis (Sunday only) $300 $500 $75
19 Advanced Metabolomics: Bioinformatics, Activity & Systems Biology (Sunday only) $300 $500 $75

* To register as a member, ASMS dues for 2019 must have been processed by ASMS prior to conference registration.
** To qualify for the student rate, you must be enrolled full-time in a degree program up to and including the Ph.D. at the time of the conference Enter short course amount $ ___________
Spouse/Companion includes Welcome Reception on Sunday and name badge. Does not give access to scientific talks, posters or exhibits. Enter $10. Enter companion amount $ ___________
Closing Event Thursday, June 6. $40 per registrant. (Limit one ticket per registrant & one ticket per spouse/companion,
see below for spouse/companion closing event ticket) Closing Event amount $ ___________
Closing Event for Spouse/Companion Thursday, June 6. $40 per registered spouse/companion. Closing Event for spouse/companion amount $ ___________
Separate Spouse/Companion registration ($10) is required.
ENTER TOTAL AMOUNT $ ___________
Payment Method
 I have enclosed a check or money order payable to ASMS for the TOTAL AMOUNT. Purchase orders or money transfers cannot be accepted. Checks must be payable in U.S. dollars and through a U.S. bank.
 Please charge the TOTAL AMOUNT to my Visa, MasterCard, or AMEX.

Visa, MasterCard, or Amex Number Security Code Expiration Date

Card Holder’s Name (please print) Card Holder’s Signature

Send this form with payment to: ASMS Conference, 2019 Galisteo Street, Building I-1, Santa Fe, NM 87505
Email to info@asms.org or Fax: (505) 989-1073 (Email or Fax registrations must include credit card information.)
TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019
Go to www.asms.org for more detailed descriptions, sample slides, and registration.

01 Bioinformatics for Protein Identification Instructors: Mark Hayward (ITSP Solutions and Active Ingredient
Bioinformatics tools are routinely used to identify proteins from Technologies), Mike S. Lee (Milestone Development Services),
“shotgun” LC-MS/MS data sets. These tools, however, are often Naidong Weng (Janssen Research and Development), Mingshe
poorly understood by their users. This course seeks to familiarize Zhu (Bristol-Myers Squibb)
proteomics researchers with the inner workings of the software
that enables this field. The course will emphasize the key steps 04 Glycans and Glycoproteins in Mass Spectrometry
in protein identification: peptide-spectrum matching, error rate This course is designed for scientists who want to learn specific
estimation, and protein assembly. It will build on these topics techniques for the MS and MS/MS characterization of glycans
by discussing advanced techniques for MS/MS identification and glycoproteins. The course will address fundamental
through spectral libraries and algorithms that leverage de novo aspects of glycobiology, sample preparation and handling,
sequence inference. Live demonstrations of the Trans-Proteomic mass spectrometry (hardware and software), and bioinformatic
Pipeline, ProteoSAFe, and BumberDash / IDPicker tools will tools for interpretation of results. Real-world examples of the
familiarize participants with the decisions that produce reliable application of these techniques will include characterization
results from these systems. Participants will emerge from the of intact glycoproteins, characterization of released glycans,
course with a solid understanding of the underlying algorithms analysis of complex mixtures of glycoproteins and glycans. The
that enable protein identification as well as the software available role of MS-based methods in interdisciplinary efforts to solve
to accomplish this task. these complex problems will also be addressed.
Instructors: Nuno Bandeira (University of California San Diego), Instructors: Jon Amster (University of Georgia), Carlito Lebrilla
Alexey Nesvizhskii (University of Michigan Health System), David (University of California, Davis), Ron Orlando (University of
Tabb (Stellenbosch University) Georgia), Joe Zaia (Boston University)

02 Clinical Diagnostics: Innovation, Validation, 05 High Resolution Mass Spectrometry for Qualitative and
Implementation and Operation by Mass Spectrometry Quantitative Analysis: An Introduction
This course will cover aspects of mass spectrometric analysis as This introductory course is designed to teach participants
applied to clinical diagnostics. Rather than approach the use of the utility, advantages, and limitations of conducting LC-
diagnostic mass spectrometry from the ground-up, the course MSn analysis using high resolution mass spectrometry. The
will focus on those components which are singular to the clinical fundamental concepts of high resolution and accurate mass
diagnostics industry. Attendees will be provided with details and measurement will be developed, as will techniques for mass
practical examples of laboratory operations in an environment measurement and mass calibration. Current high resolution
which is regulated by the FDA Office of In Vitro Diagnostics (FDA- instrument options including time-of- flight (TOF) and Fourier
IVD), College of American Pathologists (CAP) and the Clinical transform mass spectrometry (FTMS) will be described and
Laboratory Improvement Act (CLIA). compared. Mass analyzer options will be extended into MS/
Instructors: Matthew Crawford, Brian Rappold and Chris Shuford MS platforms including hybrid instruments such as Q-TOF and
(LabCorp) LTQ-FTMS. Qualitative and quantitative applications of high
resolution mass spectrometry will be discussed, especially for
03 DMPK: Experimentation and Data Interpretation small-molecule pharmaceutical compounds, biologics (antibody
Mass spectrometry has become the dominant tool throughout drug conjugates, peptides, and proteins) and biomarkers.
the drug discovery / development continuum. This short course These applications will include molecular formula and structure
will provide a thesis on mass spectrometry in drug metabolism, analyses such as identification of metabolites, as well as effects
pharmacokinetics (DMPK), and pharmacodynamics (biomarker) of high resolution on sensitivity and specificity in quantitation.
in support of R&D and the registration process. The course will Instructors: Ragu Ramanathan, Matthew Blatnik, Cong Wei (Pfizer)
use case studies to focus on the “why” and “how” knowledge
base with regard to the use of mass spectrometry to measure
small molecule drugs, biologics, and their conjugates in the
discovery and development phases. Contents will include an
introduction to the concepts / principles of DMPK, an overview of
drug discovery / development processes, regulatory submission
requirements, and common practices in DMPK studies. Current
mass spectrometry technologies applied in ADME screening
in lead optimization, drug quantification in PK studies, drug
metabolite identification in animals and humans, as well as
GLP bioanalysis quantification in clinical and toxicology studies
will be discussed along with updated industry practices for
experimental design, data interpretation, and data reporting.
Practice sessions will be given to reinforce data analysis
techniques learned in class.

www.asms.org
TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019

06 Understanding, Optimizing and Applying LC/MS/MS instrument maintenance and repair skills, which are critical for
Techniques using Electrospray, APCI and APPI to Develop limiting downtime and providing cost savings to any laboratory.
Successful Methods Instructors: Susan Abbatiello (Northeastern University),
This course is designed for the chromatographer / mass Thomas Blau (Ion Technology Support, Inc), Will Thompson
spectrometrist who wants to be successful in developing (Duke University)
methods, optimizing methods and solving problems using LC/
MS. The course covers the atmospheric pressure ionization 09 Native Mass Spectrometry
(API) techniques of electrospray, pneumatically assisted Native mass spectrometry is a growing area in the mass
electrospray and atmospheric pressure chemical ionization spectrometry community. Native MS is making significant
(APCI) and atmospheric pressure photo ionization (APPI) using contributions to structural biology and because of its ease of
single quadrupole, triple quadrupole, time-of-flight and ion application and broad applicability, often leads and guides
trap mass analyzers. Discussions of sample preparation and an integrated approach that eventually uses other structural
modes of chromatography will target method development biology tools. This course is designed to introduce the broad field
and optimization for the analysis of “real-world” samples by LC/ to new practitioners of native MS. Topics to be covered include
MS. The course highlights the following topics with respect to sample preparation for native MS; instrumentation used for
optimization methods to achieve the best sensitivity, specificity native MS - how does it differ from other MS; spray conditions to
and sample throughput. keep soluble complexes native; spray conditions for membrane
Instructor: Robert D. Voyksner (LCMS Limited) protein complexes (micelles, liposomes, nanodiscs); activation
methods for native MS (CID, CIU, SID, UVPD, ETD/ECD/EID);
07 LC-MS: Advanced Techniques and Applications complementary methods (HDX, covalent labeling, crosslinking,
This two-day course presents a comprehensive overview of top-down MS); ion mobility for native MS; online separations
technology and techniques of analytical mass spectrometry approaches coupled to native MS (IEX, SEC, CE); software tools
and from that foundation extends into exciting, disruptive for native MS; (Unidec, Impact, Pulsar, EMnIM, DynamXL, XLinkX,
recent developments. Course will include sample preparation; iFAMS); case studies for protein:protein, protein ligand, RNA:
advanced separation techniques; ionization techniques for MS; protein, DNA:protein, and membrane protein complexes.
mass Analyzers; imaging and profiling by MS; high resolution Instructors: Vicki Wysocki (The Ohio State University), Sophie
MS; miniaturization in MS; and synergistic integration. Harvey (The Ohio State University), David Russell (Texas A&M
Instructor: Jack Henion (Advion, Inc.) University), Arthur Laganowski (Texas A&M University)

08 LC-MS: Practical Maintenance and Troubleshooting 10 Peptides and Proteins in Mass Spectrometry
With expanding use of LC-MS in laboratories not specifically Over the past two decades, mass spectrometry has become the
focused or trained in analytical chemistry, the analytical skills key technology for the characterization of proteins. This course is
needed to maintain high instrument performance and data designed as an introduction for researchers needing to expand
quality are less often part of the central curriculum or training of their knowledge of the use of mass spectrometry-based methods
instrument operators in these laboratories.  Indeed, high-end LC- for the identification, characterization, and quantification of
MS systems are now routinely found far outside of the traditional peptides and proteins. Background material in basic protein
analytical chemistry laboratory, including labs focused on chemistry will be provided along with a review of mass analyzers,
pharmaceutical, biochemical, biological, or environmental acquisition types, and ionization sources used for protein and
applications. This is a natural progression as LC-MS technologies peptide analysis. Real-world examples will be used to illustrate
become more mature and robust, however the increased use protein sample preparation strategies, characterization of intact
of LC-MS has not removed the need for basic troubleshooting, proteins, characterization of post-translational modifications
(with emphasis on glycosylation and phosphorylation),
identification of proteins via database searching, direct
identification of proteins from gels and complex mixtures, and
quantitative differential protein expression studies. The role of
MS-based methods in interdisciplinary efforts to solve complex
biomedical problems will also be addressed. Additionally, there
will be tutorials on the use of open source proteomic software
tools for interpretation of proteomic datasets. Problem sets
will be used to emphasize practical aspects of comprehensive
protein characterization, including peptide sequencing and
protein identification by database searching.
Instructors: Arthur Moseley (Duke University), Douglas Sheeley
(NIH), Eric Soderblom (Duke University)

www.asms.org
TWO-DAY SHORT COURSES • ATLANTA
Saturday and Sunday, June 1 and 2, 2019
11 Protein Structural Analysis by Mass Spectrometry: instrument and assay optimization procedures; (d) real world
Hydrogen Exchange and Covalent Labeling approaches to assessing problems and improving method
This course is designed for those who wish to understand protein ruggedness; (e) general guidelines for method validation in
structure with mass spectrometry. a regulated environment; (f ) an introduction to quantitation
We will focus on covalent labeling for half of the course and on of endogenous compounds in biological matrices; and (g) an
hydrogen exchange for the second half. There will be a discussion introduction to quantitation in proteomics. Ample time will be
of the theory behind each labeling method as it relates to provided for general discussion of the topics presented and
proteins in solution, the general methodological steps one takes other current topics in quantitative MS.
to do these analyses, and a guide on how to process and interpret Instructors: Dwight E. Matthews (University of Vermont) and
the resulting data. There will be a discussion of the pitfalls one Robert Bethem (Consultant)
can encounter during these experiments. Specific examples of
protein structure analysis will be provided in applications like 14 Case Studies in Quantitative Proteomics
enzymology, protein-ligand interactions, protein dynamics, and This course will explore practical aspects of setting up and
membrane proteins. Course participants will receive a copy of performing a quantitative proteomics experiment. The course
a book from Wiley edited by the course instructors providing will focus on how to develop methods and perform the
detailed additional background on all the approaches. analysis using the Skyline Software Ecosystem (http://skyline.
Instructors: Mark Chance (Case Western Reserve), Roxana Iacob ms). Specifically, we will cover practical issues we have faced in
(Northeastern University), Janna Kiselar (Case Western Reserve), performing data collection and in the interpretation of data using
Thomas Wales (Northeastern University) case studies from our own labs. At the end of the course each
participant will be familiar with introduction to quantitation of
12 Protein Therapeutics: Practical Characterization and proteins by mass spectrometry; strategies for peptide selection
Quantitation by Mass Spectrometry as a proxy for a protein; using Skyline for method refinement and
This introductory course is designed for practicing analytical data analysis; automated strategies for LC-MS system suitability
scientists (new users, chromatographers, analytical chemists, monitoring; visualization and assessment of multiplex peptide
protein chemists, and laboratory managers) performing/ LC-MS/MS data; sharing data using Panorama; assessing assay
supporting recombinant protein characterization/analysis, in- figures of merit; different mass spectrometry data acquisition
process testing, quality control, quality assurance, research and strategies used in proteomics.
development, and manufacturing. It covers the fundamental Instructors: Meena Choi (Northeastern University), Jake
principles and practical applications of mass spectrometry (MS) Jaffe (The Broad Institute), Brendan MacLean (University of
for the characterization and quantitation of protein therapeutics Washington), Olga Vitek (Northeastern University)
with a focus on monoclonal antibodies including antibody-
drug conjugates (ADCs). It emphasizes problem-solving skills 15 Untargeted Metabolomics: From Basic Methods to
in the areas where MS is used most extensively, for example, Advanced Workflows and Isotope Labeling
characterization of chemical modifications and post-translational It has become relatively routine to acquire mass spectrometry-
modifications of protein therapeutics. The interpretation of based metabolomic data, either in one’s own laboratory or using
protein therapeutics mass spectra will be illustrated with real one of the many service facilities around the world. Despite this
case examples. This course will focus on electrospray ionization progress, however, interpreting metabolomic results continues
(ESI) and matrix-assisted laser desorption / ionization (MALDI) to be a major challenge for many researchers that severely
techniques in the analysis of protein therapeutics, and will limits potential applications of the technology. The overarching
survey various mass analyzer options for the characterization of objective of the two-day metabolomics short course is to guide
protein therapeutics. A thorough coverage of approaches toward students to overcome this critical interpretation barrier.
method development for both qualitative and quantitative Instructor: Gary Patti (Washington University-St Louis)
analysis of protein therapeutics will provide a good starting
point for understanding practical issues that are frequently
encountered during implementation and applications of MS on
protein therapeutics in the laboratory.
Instructors: Guodong Chen and Li Tao (Bristol-Myers Squibb)

13 Quantitative Mass Spectrometry


This introductory/intermediate level course explores the
principles of quantitative mass spectrometry as they apply
primarily to small molecule analyses. Topics that will be discussed
include: (a) MS instrumentation used for quantitative analyses;
(b) how a quantitative assay is set up including statistics for
quantitation, preparation and evaluation of standard curves
and analysis of variance; (c) LC/MS/MS method development
for target quantitative analysis of small molecules, including

www.asms.org
ONE-DAY
ONE-DAY SHORT
SHORT COURSES
COURSES • ATLANTA
Sunday, June 2, 2019
Go to www.asms.org for more detailed descriptions, sample slides, and registration.
17 Introduction to GLP Regulations and Bioanalytical 19 Advanced Metabolomics: Bioinformatics,
Method Validation by LC-MS/MS Activity & Systems Biology
Sunday only Sunday only
“GUIDANCE FOR INDUSTRY/Bioanalytical Method Validation” The field of metabolism has had resurgence due to major
represents the Food and Drug Administration’s current thinking technological advances in mass spectrometry, which has
on this topic and was first published in May 2001. Since then, enabled new insights into the involvement of metabolites in
almost all regulated bioanalytical methods have been validated biological processes and enzymatic pathways. It is one of the
based on the guidance even though it does not create or fastest growing areas of science today and its primary analysis
confer any rights for or on any person and does not operate tool is mass spectrometry. The Advanced Metabolomics course
to bind FDA or the public. In September 2013, FDA published will complement the other ASMS Metabolomics short course
a draft guidance, which provides general recommendations for going into greater depth on key informatic topics that are critical
bioanalytical method validation using advanced technologies. to scientists trying to establish themselves in the field. Topics
The content of the original guidance issued in 2001 was revised to include metabolomics activity screening, assessing statistically
reflect advances in science and technology related to validating relevance, pathway analysis, and systems biology guided by
bioanalytical methods. This one-day short course will focus on metabolomics.
GLP regulations and the bioanalytical method validation for Instructors: Gary Siuzdak, H. Paul Benton, Xavi Dominguez and
drugs and metabolites in biological matrices using LC-MS/MS. Erica Majumder (The Scripps Research Institute)
It will help audiences to comply with FDA’s regulations for drug
discovery and development in the pharmaceutical industry
and CROs. The short course will also reflect the contents of the
updated guidance and recently published white papers with
regard to bioanalytical method validation using LC-MS/MS.
International harmonization of bioanalytical method validation
guidance will be discussed.
Instructor: Perry Wang (LC-MS Technical Expert)

18 Ion Mobility Mass Spectrometry: An Introduction to


Instrumentation, Applications, and Data Analysis
Sunday only
Realization of the value-added by combining ion mobility
spectrometry (IMS) and mass spectrometry (MS) has generated
significant interest in its use in range of fields including omics
pursuits, threat detection, and fundamental studies of gas-
phase ion chemistry. This course provides students with a basic
understanding of the most common instrumental configurations,
experimental sequence, best practices, and the theory underlying
the different types of ion mobility systems employed both in
academic and commercial settings. An overview of hyphenated
IMS-MS instrumentation with multiple practical applications and
experimental designs will be presented including comparative
discussions on advantages and disadvantages between different
stand-alone and hyphenated techniques. This course will provide
a comprehensive look at ion mobility spectrometry and its use
in modern analytical chemistry. While ion mobility systems are
largely compatible with all mass spectrometry systems each IM-
MS combination possesses a range of unique advantages and
applications. These instrumental considerations and specific
experimental sequences will be covered in this course. Graduates
of the course will have gained a detailed understanding of IMS
and an overview of its practical applications for both stand-alone
and MS-coupled operation.
Instructors: Erin Baker (North Carolina State University), Brian
H. Clowers (Washington State University), John A. McLean
(Vanderbilt University)

www.asms.org