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This exam consists of 40 multiple choice questions worth 2.5 points each.
On the separate testing form, please fill-in the single best choice for each
question. Be sure to fill-out your student number and name on the answer sheet.
Good luck!
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1. DNA glycosylases
2. methylation patterns
3. 3’ 5’ exonuclease activity
4. AP endonucleases
1. frameshift.
2. base analogue.
3. transversion.
4. transition.
4. A tautomeric shift
1. photoreactivation in humans.
2. excision repair in humans
3. oxidative damage.
4. telomerase.
1. uracil
2. adenine
3. guanine
4. phosphorylated
1. percent recombination.
2. RFLPs.
3. centiMorgans.
4. base pairs.
10. Which technique would be used to make a genetic map of the genome of
Mendel’s pea plants?
1. dihybrid cross with two linked genes
2. map-based sequencing
3. restriction mapping
4. shotgun sequencing
12. The genome sequences of four bacterial species are compared for the presence
or absence of eight proteins. What can you conclude from the data in the table below?
14. Based on the most recent analysis of the sequence of the human genome, which
is closest to the estimated number of human genes?
1. 3,000
2. 30,000
3. 100,000
4. 500,000
15. A fragment of DNA was sequenced and the gel pattern shown above was
obtained. For the strand of DNA that was synthesized in the sequencing reaction, what
base was incorporated first (closest to the primer)?
1. G
2. A
3. C
4. T
16. What was the sequence of the DNA strand that acted as the template in the
sequencing reaction?
1. ambiguous.
2. overlapping.
3. not present in mRNA.
4. not absolutely universal.
21. Imagine a world in which proteins are made from 65 different amino acids.
Assume that in this imaginary world the genetic code is nonoverlapping and
utilizes the smallest possible size for a codon that accommodates all amino
acids unambiguously. In this particular world, which of the following mutations in
the coding region of a gene would not cause a frame-shift?
1. an insertion of 2 nucleotides
2. an insertion of 3 nucleotides.
3. a deletion of 8 nucleotides
4. a deletion of 5 nucleotides.
23. What is the term which refers to a contiguous set of bacterial genes which are
under coordinate control?
1. lysogen.
2. prototroph.
3. operon.
4. allosteric.
Use the following diagram of the lac I gene and lac operon for questions 26 and 27.
(I = lac repressor gene; Z, Y, A = lac operon structural genes; P = lac promoter; O = lac
operator)
I P O Z Y A
26. Where would the lac repressor be bound in a (nonmutant) E. coli cell that is
growing in low glucose and high lactose?
1. P
2. O
3. P and O
4. the repressor would not be bound
27. Which parts of the DNA region shown in the diagram encode proteins?
1. P
2. P, O, Z, Y, A
3. I, Z, Y, A
4. I, P, O, Z, Y, A
29. A mutant E. coli strain, grown under conditions that normally induce the lac
operon, produces high amount of ß-galactosidase. What is a possible genotype of the
cells?
1. synthesize lactose.
2. display inducible expression of the lac operon.
3. display constitutive expression of the lac operon.
4. probably not catabolize lactose.
1. tryptophan is an inducer.
2. tryptophan is a co-repressor.
3. attenuation can halt replication.
4. none of the above.
33. What modification neutralizes the charges on histones and loosens up the
interactions between histones and DNA?
1. phosphorylation
2. demethylation
3. acetylation
4. polyadenylation
1. RNA polymerase I
2. RNA polymerase II
3. RNA polymerase III
4. none of the above.
1. removal of introns.
2. shuffling of exons.
3. removal or modification of amino acids.
4. removal of exons.
36. A type of DNA sequence in eukaryotes that helps to regulate transcription, may
be tissue specific, and may exert control over a structural gene that is a great
distance away is called
1. a promoter.
2. a Shine-Dalgarno sequence.
3. a TATA box.
4. an enhancer.
1. DNase insensitive.
2. in a highly condensed form
3. usually DNase sensitive.
4. sometimes referred to as heterochromatin.
That’s All !