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capable)
PROFESSION
STEM CELL
What is a stem cell?
EUTHANASIA o Stem cell therapy is a set of techniques that aim
From Greek word means “good health” to replace cells damaged or destroyed by
A theory that states that all of us have the right to die in a disease with healthy functioning ones. The
painless and peaceful manner when we are suffering from a techniques themselves are still relatively new but
dreadful disease their applications and benefits are broad. Some
Refers to the practice of intentionally ending life In order to of the diseases they can help include:
relieve pain and suffering Cancer
Parkinson's disease
TYPES OF EUTHANASIA Diabetes
Voluntary - When the patient brings about his or her own Various injuries (e.g. spinal cord)
death with the assistance of a physician, the term assisted Eye diseases
suicide is often used instead. TYPES OF STEM CELL
Non-voluntary – where the consent of the patient is Embryonic stem cells (pluripotent)
unavailable Tissue stem cells (multipotent)
Involuntary - conducted against the will of the patient
Euthanasia by Omission or Passive - entails the withholding TISSUE STEM CELLS
of common treatments, such as antibiotics, necessary for the Known as “adult stem cell”
continuance of life. Includes stem cells isolated from fetal and cord blood
Euthanasia by Commission or Active - entails the use of lethal Reside in the most tissues of the body where they are
substances or forces to kill, such as administering a lethal involved in repair and replacement
injection Generally very difficult to isolate
Under right conditions, they can be grown in the laboratory
ACTIVE VS PASSIVE EUTHANASIA It is widely used in research for treatments of blood
Active Euthanasia is when is brought about by an act , for cancers(leukemia and autoimmune diseases)
example when a person is killed by being given an overdose CLONING
of pain killers
Passive Euthanasia is when death is brought about by an
omission, i.e., when someone let the person die. This can be TYPES OF CLONING
by withdrawing or withholding treatment: DNA Cloning or Recombinant DNA Cloning - a process in
o Withdrawing treatment – for example, switching which the biological matter that is cloned is only a small
off a machine that is keeping a person alive, so fragment of DNA
that they die of their disease Reproductive DNA Cloning or Organism Cloning – involves
o Withholding treatment – for example, not creation of an identical copy of an entire organism
carrying out surgery that will extend life for a Therapeutic Cloning (SCNT) – involves cloning of human
short time embryos for the production of stem cells. The embryos are
destroyed in this process.
INDIRECT EUTHANASIA
This means providing treatment (usually to reduce pain) that DNA CLONING OR RECOMBINANT DNA CLONING
has the side effect of speeding the patient’s death Procedures by which DNA from different species can be
Since the primary intention is not to kill , this is seen by some isolated, cut and spliced together -- new "recombinant”
people (but not all) as morally acceptable molecules are then multiplied in quantity in populations of
A justification along these lines is formally called the doctrine rapidly dividing cells (e.g. bacteria, yeast)
of double effect
REPRODUCTIVE CLONING
ASSISTED SUICIDE Reproductive cloning is the production of a genetic duplicate
This usually refers to cases where the person who is going to of an existing organism. A human clone would be a genetic
die needs help to kill themselves and asks for it. It may be copy of an existing person.
something as simple as getting drugs for the person and
putting those drugs within their reach. METHODS OF CLONING
Artificial Embryo Twinning – is a technology that mimics the
HIERARCHY OF SUBSTITUTE DECISION MAKERS natural process of identical twin creation. This is done by
Guardian of the person manual separation of very early embryo into individual cells
Lawyer of the person then allowing the cell to divide on its own. The resulting
Representative appointed by the Consent and Capacity e4mbryos are then placed into the surrogate mother, where
Board they are carried to term and delivered
Spouse or Partner SCNT (Somatic Cell Nuclear Transfer – created Dolly, the
Child or Parent or Children’s Aid Society famous sheep. Done by isolating a somatic cell from a donor
Parent having a right of access and transfer the nucleus of that cell to an enucleated egg cell.
Brother or Sister After few weeks, it will behave as fertilized egg and develop
Any other relative (related by blood, marriage or adoption) into an embryo which is implanted into a surrogate mother
and carried to term
Collecting and storing human sperm from sperm donors for
use by women who, for whatever reason, need donor-
provided sperm to achieve pregnancy
Sperm donated by the sperm donor is known as donor sperm,
and the process for introducing the sperm into the woman is
called artificial insemination, which is a form of third party
reproduction.
ZYGOTE BANKING
Embryo cryopreservation or embryo freezing is a method
used to preserve embryos by cooling and storing them at low
temperatures. They can then be thawed at a future date and
transferred to the uterus, providing additional opportunity for
achieving conception.
IVF (In Vitro Fertilization)
is the process of fertilization by manually combining an egg
and sperm in a laboratory dish, and then transferring the
embryo to the uterus.
ALTERNATIVES TO IVF
POTENTIAL APPLICATIONS
Potential benefits for medicine field and agricultural GIFT. Gamete Intrafallopian Transfer
field o Is an assisted reproductive procedure which
Some cloned sheep have been genetically altered to involves removing a woman’s eggs, mixing them
produce milk containing human protein important for with sperm, and immediately placing them into a
blood clotting which could someday be helpful once the fallopian tube.
protein can be purified from the milk ZIFT. Zygote Intrafallopian Transfer
Testing of new drugs and treatment strategies o Is an assisted reproductive procedure similar to
Build populations of endangered or extinct species of in vitro fertilization and embryo transfer, the
animals difference being that the fertilized embryo is
transferred into the fallopian tube instead of the
DRAWBACKS OF CLONING uterus.
Expensive Embryo Cryopreservation (frozen fertilized egg and sperm)
Highly inefficient
Most cloned embryos cannot develop into healthy beings LIMS / LIS / LMS
Serious health effects: premature aging, short life span, A laboratory information management system (LIMS),
increase in birth size, defects with immune system, defects in sometimes referred to as a laboratory information system
essential organs (liver, brain and heart) (LIS) or laboratory management system (LMS), is a software-
based laboratory and information management system with
SPERM BANKING features that support a modern laboratory's operations.
“Cryopreservation” Database applications that are used to store and manage
Freezing and preserving sperms at -196C or -320F information associated with the laboratory. They contain
Standard average storage time: 10 years information such as:
Means providing a semen sample for freezing o Customers
o Collection Sites ADDITIONAL FUNCTIONS
o Preservatives Personnel and Workload Management
o Analysis Methods o Organize work schedules, workload
o Employees assignments, employee demographic
o Passwords information, training, and financial information
o Test and Test Results Quality Assurance and Control
o Bottle Types o Gauge and control sample quality, data entry
o Sample Matrix standards, and workflow
o Parameters Reports
o Control Limits o Create and schedule reports in a specific format;
schedule and distribute reports to designated
LIMS KEY FEATURES parties
Workflow and Data tracking Time Tracking
Flexible architecture o Calculate and maintain processing and handling
Data exchange interference times on chemical reactions, workflows, and
more
BENEFITS OF LIMS Traceability
Very fast report access and queries o Show audit trail and/or chain of custody of a
Reduction in paperwork sample
Improved data quality Workflows
Improved operational efficiency o track a sample, a batch of samples, or a "lot" of
Increased productivity batches through its lifecycle
Reduces transcription errors
Avoids duplication DISTINCTION BETWEEN A LIMS AND A LIS
Increases throughput LIMS LIS
LMS handle complex batches of LIS for individual patients in
QC BENEFITS data in biology labs, water clinical setting
Track training records and employee certifications treatment facilities, drug trials
Track instrument maintenance and calibration Needs to satisfy GMP Must satisfy the reporting and
Full audit/trend and QC charts auditing needs of hospital
Automatic limit checking accreditation agencies
Automatic sample scheduling Most competitive in group centric Most competitive in patient-
Auto reporting settings centric settings and clinical labs
Often deal with mostly
anonymous research specific
SAMPLE MANAGEMET
laboratory data
Some LIMS will allow the customer to place an "order" for a
sample directly to the LIMS at which point the sample is
generated in an "unreceived" state. HAZARDOUS HEALTHCARE WASTES
The processing could then include a step where the sample Any residues or combination of residues other than
container is registered and sent to the customer for the radioactive waste which by reason of its chemical reactivity
sample to be taken and then returned to the lab. or toxic, explosive, corrosive or other characteristics causes
The registration process may involve accessioning the potential hazard to human or environment, either alone or
sample and producing barcodes to affix to the sample when in contact with other wastes and which therefore cannot
container. be handled, store, transported, treated or disposed of without
Various other parameters such as clinical or phenotypic special precautions.
information corresponding with the sample are also often
recorded. CLASSIFICATION OF HAZARDOUS HEALTHCARE WASTE
The LIMS then tracks chain of custody as well as sample Infectious waste
location. Location tracking usually involves assigning the o waste is suspected to contain pathogens and
sample to a particular freezer location, often down to the that poses a risk of disease transmission
granular level of shelf, rack, box, row, and column. Pathological waste
LIMS users may also have regulatory concerns to comply o Human tissues, organs or fluids; body parts;
with such as CLIA, HIPAA, GLP, and FDA specifications, fetuses; unused blood products
affecting certain aspects of sample management in a LIMS Sharps
solution. o Considered highly hazardous items that can cut,
unused or used sharps
ELECTRONIC DATA EXCHANGE Pharmaceutical waste
The successful transfer of data files in spreadsheet and other o Expired products, split or rejected by the patient
formats, as well as the import and export of data to MySQL, for medication (e.g. drugs, vaccine)
PostgreSQL, and other databases is a pivotal aspect of the Cytotoxic or Genotoxic waste
modern LIMS o Highly hazardous waste which includes vomit,
feces and urine from the patient treated with
cytostatic drugs (often used in CA therapy)
Chemical waste
o Waste containing chemical substances
o Corrosive (e.g. Acids of pH <2 and bases of pH
Radioactive waste <12)
o Waste containing radioactive substances o Flammable
INFECTIOUS WASTE o Reactive (explosive, water reactive, shock
Infectious waste is suspected to contain pathogens (bacteria, sensitive)
viruses ,parasites, or fungi) in sufficient concentration or
quantity to cause disease RADIOACTIVE WASTE
In susceptible hosts. This category includes: Radioactive waste are materials contaminated with
o Waste contaminated with blood or other body radionuclides. They are produced as a result of procedures
fluids such as in vitro analysis of body tissue and fluid, in vivo organ
o Cultures and stocks of infectious agents from imaging and tumor localization and various investigative and
laboratory work therapeutic practices
o Wastes from infected patients in isolation wards. Radionuclides used in health care are
FIRE HAZARDS
Fire is the rapid oxidation of a material in the exothermic
process of combustion releasing heat, light and various
reaction products
ELECTRICAL HAZARDS
Includes autoclaves, vacuum and pressure vessels, gas
cylinders, freezers, liquid nitrogen containers and all electrical
equipment in the laboratory