Paco Catholic School

1521 Paz Street Paco, Manila

High School Department

PAASCU Accredited Level III

S.Y. 2019-2020

“The Effect of the Organic Biostimulant from Leftover Waste of Tilapia on the Growth of
Chili Plant.”

Submitted to:

Mrs. Camposano

Group 3 12-STEM 3

Canlas, Kian Therese

Dimalibot, Quincy

Dela Cruz, Aoshi

Caurez, Rusty

De Dios, Lawrence

August 13, 2019

Chapter I: Introduction

A. Background of the Study

Tilapia is likely the most established homestead brought fish up around the world. Tilapia is a

tough herbivorous fish that feeds on green growth or little oceanic plant cells, and is essentially

brought up in freshwater frameworks utilizing confines, lakes, raceways or vast waters. The

water conditions in the cultivating tasks importantly affect item quality and taste. Tilapia has

been known as the "water chicken" as a result of the rearing enhancements and large scale

manufacturing strategies that summon correlations with the land based chicken industry.

(National Institute of Food and Agriculture, U.S. Department of Agriculture, 2018). Tilapia

became the second most popular fish in the Philippines. In the article "the fishing industry of the

Philippines" (Mendoza, 2019), 17% of total Freshwater fish cage in Batangas is due to tilapia,

98% of the total freshwater fish pond is also tilapia. In addition, Batangas contributes high value

of fishes because of tilapia; it reached the top 3 of aquaculture production and top 1 in

freshwater fish cage with 74,946 MT.

As expressed in the article of Aquaculture Research of Sanchez, Vivian‐Rogers, and Urakawa

(May 2019) titled" Tilapia recycling aquaculture frameworks as a wellspring of plant

development advancing microscopic organisms", tilapia waste comprise of microorganism that

encourages the plant to adjust and keep up its development and solid root which become more

significant than other compost. Microbial social order chose using high‐throughput sequencing

demonstrated the estimation of Bacteroidetes and Alphaproteobacteria as beneficial microbial

pointers to study the prosperity condition of reusing aquaculture structures. The researchers

discovered that tilapia waste have "Actinobacteria", a naturally significant gathering and

generally found in earthly and amphibian environments that assume to have a significant role in
a few organic procedures, for example, biogeochemical cycles, bioremediation, bioweathering

and plant development advancement. (Kandiah, Jeevanantham, et al., 2019)

Based on the Department of Agriculture, Regional Field O Ce No. 02, High Value Crops

Development Program, Pepper is one of the common vegetables because aside from we can

see it anywhere, it is also one of the basic ingredients that people use in cooking. It is a well-

known vegetable element for Filipino dishes. It is utilized in the planning of sauces, pickles and

as avoring element of different plans. Hot pepper, that has a scientific name of Capsicum

frutescens, is a favorite in food because of its aroma. It is being developed for its medicinal and

pharmaceutical properties. It eases torment in patients that has arthritis and helps in lowering

the risk of diabetes. Hot chili pepper grows faster in sandy loam that is rich in organic

substances and it is normally planted from May to September during the wet season and

October to February during the dry season. (Tubbun, H., 2017). Hot peppers are set up in

roughly 60 to 95 days in the wake of planting. Pick hot peppers when they have touched base at

full stature which is shrubs 1½ to 2 feet tall and their create shading from green to red to gold

and yellow. Hot peppers can go long from 1 to 7 creeps long and in concealing. (Albert, S.,

2016).

One of the traditional strategies for the reuses of fish waste are creature nourishes and treating

the soil it into manure with the utilization of biotechnology to diminish fish squander that will

support the natural issues. Fish compost comprise of extra misuse of a fish, for example, its

organs or tissues, bones and scales joined with various substrates including sugar, jaggery and

palm jaggery and transformed into fluid manure. It additionally has characters, for instance,

smell, physical state, weight, pH and protein substance were assessed. (Suganya, Renuka,

Saravanan, and Elavarasi, February 2015)

This study seeks to know the use the leftover waste of tilapia through an organic biostimulant

that will have an effect on the growth of chili plant. Fishery is one of the main sources of income

in our country especially in the Province of Lipa in Batangas that have various records of high

production that helped the researchers in thinking of using its leftovers as biostimulant on chili

plants that will not just help agriculturally but also financially.
 B. Scope and Limitations of the Study

This study focuses on The Effectiveness of Organic Biostimulant made from Leftover Tilapia

Waste to the Growth of a Chili Plant. This research aims to inform people on how the leftover

parts of a tilapia can be used as an organic biostimulant.

The researchers will conduct an experiment with 4 set-ups.First, Plant A which is the controlled

set-up that will have commercial fertilizer instead of the organic biostimulant, Second, Plant B

which will have 10 g of the Organic Biostimulant, Third, Plant C which will have 15 g of the

Organic Biostimulant, and lastly, Plant D which will have 20 g of the Organic Biostimulant. The

experiment will be done in two months and are to be measured only when the biostimulant is

mixed in the soil. The chili plant will be measured every three (3) days five (5) times. The

experiment will be done in the researcher's house.

 C. Significance of the Study

The study is beneficial to the following:

 Researchers- will benefit by the knowledge of this research and how to use the leftover

waste of the tilapia and to help other people and nature.

 Fish Vendors-will benefit by selling the organs and bones for the people who need for

their plants instead of throwing it away.

 Chili Plant Producers- will no longer use other chemicals to give plants’ nutrients to grow

better and the producers will pay low amount of money to buy a fertilizers for their plants.

 Agricultural Sector of the Local Government of Lipa, Batangas -will no longer need to

throw the leftover waste organs and bones on the fish markets because the waste is

needed for the plants and they will benefit by the fertilizer by not paying high cost of the

chemical fertilizer to increase of the chili plant production.

 People in Lipa, Batangas-will not to throw the organs and bones of tilapia because the

leftover waste will help the chili plants to grow better and have more nutrients and can

also help for extra income.

 D. Statement of the Problem:

This study seeks to answer the following question:

1) What amount of leftover waste of Tilapia specifically the organs and bones are needed to

make a successful biostimulant?

2) Which of the physical attributes of the chili plant is greatly affected by the organic

biostimulant?

3) What amount of biostimulant improves the physical attributes specifically the height, odor and

color of the chili plant?

E. Hypothesis:

If the amount of biostimulant increases then the growth of the chili plant increases.
 F. Review of Related Literature

Based on European Biostimulants Industry Council, (n.d.), Agricultural biostimulants include

various formulations of compounds, substances and micro-organisms that are applied to plants

or soils to improve qualities like crop vigor, yields, quality and sensitivity to abiotic stresses and

has the following benefits: improves the effectiveness of the plant’s metabolism to

stimulateincreased crop yield and improved crop quality, increases plant tolerance and revival

from abiotic stresses, facilitates nutrient absorption, translocation and use, enhances quality

attributes of produce, including sugar content, color, fruit seeding, etc; renders water use more

efficient, and develops soil fertility, particularly by nurturing the improvement of complementary

soil micro-organisms.

Rabe, M. E., (2011) from Inquirer.Net reported that there are no signs of abating; the Taal Lake

fish kill has spread to two more towns and a city over the weekend. Local authorities on Sunday

reported tons of farmed and endemic fish species turning belly up in new areas of the

freshwater lake, just hours after they were done emptying some of the worst-hit fish cages of

rotting stock from last week’s devastation.

A review article from Frontiers in Plant Science (2018) with the title, “PlantGrowth-Promoting

Rhizobacteria: Context, Mechanisms of Action, and Roadmap to Commercialization of

Biostimulants for Sustainable Agriculture” stated that the rhizomicrobiome is of great

significance to agriculture due to the rich diversity of root emission discharge and plant cell

remains that attract diverse and unique patterns of microbial migration. The microbes and

compounds they secrete comprise valuable biostimulants and play key roles in modulating plant

stress responses. This review article is to inform about the role of PGPR in agriculture, from

their collection to commercialization as low-cost commercial agricultural inputs.

Yap Jr., J. (n.d.) from Allied Botanical Corporation claimed that a practical farmer from Barangay

San Fernando in Sto. Tomas, Batangas, was able to harvest at least one-ton or 1,000 kilos of

chili pepper per month from a 2,500-square meter farmland and he is Elmer L. Umali that said

that his bountiful harvest was realized after cultivating almost 10,000 plants of the Pinatubo F1

hybrid pepper variety in his farm.

According to a research article from Biology Open (2019) entitled “Characterization of plant

growth promoting rhizobacteria and their benefits on growth and phosphate nutrition of faba

bean and wheat” thatin current years, more attention has been paid to plant growth promoting

(PGP) rhizobacteria use as a biofertilizer alternative to chemical fertilizers, which might cause

harm to the environment. The main purpose of this work was to evaluate the field application of

PGP bacteria and rhizobial strains on the productivity of two food crops extensively used in

Morocco; Vicia faba L. and Triticum durum L. A field experiment with four treatments was

designed: (1) control without immunization, (2) PGP bacteria alone (P), (3) rhizobia alone (R)

and (4) a mixture of PGP-rhizobia (PR).Inaddition the PGP strains were tested for their ability to

solubilize intricate mineral phosphorus and potassium and for their creation of indole acetic acid

and exopolysaccharides.

TheSpruceEats.com (2019) defined capsaicin as the chemical in chili peppers that makes them

spicy. Specifically, capsaicin occurs in the fruits of plants in the Capsicum family,

including jalapeño peppers, cayenne peppers, and other chili peppers and it may also arouse

the production of endorphins, which is why some people report experience a sense of euphoria

when eating spicy foods and a number of non-culinary applications, including as a pain reliever

and as the active ingredient in pepper spray.

Russel, P. (2011) from Groundtoground.org wrote that the fish fertilizer can be effective organic

way to grow healthy plants but beware of advertising fish and toxins of fish from polluted waters
because it might not be good for your plants so it’s better to used the organic waste of the fish to

achieve the healthy result of your plants.

According to gardeningknowhow.com (2018) Composting fish has long been use by Native

Americans whey they planting corn seeds encourage healthy yields. Basic needs for creating

organic waste of fish are source of carbon and nitrogen which is where the fish scraps come in

to play. A simple recipe is three parts carbon to one part nitrogen which is where the fish scraps

comes in to play.

A simple recipe is three parts carbon to one part nitrogen in the article made by Xu, L. &Geelen,

D. (2018) stated that in the first step toward developing biostimulants from organic waste, the

choice of biomass resource is critical. Various active ingredients found in industrial waste

streams and by-products of biological origins pose the perfect opportunity to extract molecules

for better growth and pathogen resistance of valuable crop plants.

According to Europepemc.org (2019) boistimulants means a material that contains substance or

microorganisms it will function when you apply it to a plants or the rhizosphere is to stimulate

natural processes to benefit nutrients uptake.

In the article made by Yakahin, O. Lubyanov, A. & Brown, P. (n.d) Biostimulants are made to

develop the condition of the plant not only the nutrients improve in putting biostimulant but also

growth regulators or the protective compounds of plant.

According to Colla, G., Hoagland L, and Rouphael, Y. (n.d) Plant-derived protein hydrolysates

(PHs) have gained dominance as plant biostimulants because of their potential to increase the

germination, productivity and quality of a wide range of horticultural and agronomic crops.

According to the study of Suganya, Renuka, Saravanan, and Elavarasi (February 2015) titled

“Studies on turning fish waste into gardening fertilizer”, there’s an increase of fish consumption
of every person in the world therefore, the leftover waste of fish also increases. Fish waste

produces different environmental problems including odor problems, floating debris and other

disposal problems. One of the conventional methods for the reuse of fish waste is animal feeds

and composting it into a fertilizer with the use of biotechnology to reduce fish waste that will help

the environmental issues. Fish fertilizer consist of leftover waste of a fish such as its organs or

tissues, bones and scales combined with different substrates including sugar, jiggery and palm

jiggery and turned into liquid fertilizer. It also has characters, for example, smell, physical state,

weight, pH and protein substance were evaluated. Manure worth was evaluated by breaking

down the blossoming impact and delicate leaf arrangement of rose plant. Following 48 days,

awful scent transformed into fruity smell in fish waste blended with natural sweetener, stick

jiggery and palm jiggery. The sprouting of blossom in the rose plant was expanded in the wake

of applying the fertilizer. In conclusion, there is a need to discover naturally worthy methods for

reutilization of these fish waste.

Base from the article of Steve Albert, (2016) entitled “how to grow hot chili peppers), hot

peppers are prepared in approximately 60 to 95 days after planting. Pick hot peppers when they

have arrived at full height which is bushes 1½ to 2 feet tall and their develop color from green to

red to gold and yellow. Hot peppers can go long from 1 to 7 inches in length and in shading.

Peppers are delicate perennials that are developed as annuals. Peppers develop on compress

erect. The organic product pursues a solitary flower developing in the edge between the leaf

and the stem. In addition, hot peppers are good in responding to organic biostimulant especially

animal fertilizer and other waste compost where in it will get enough sources of nutrients that a

chili plant need.

According to Vicky Viray-Mendoza an executive Editor, Maritime Review Magazine (2019),

Batangas become part of the top 10 in Aquaculture production due to tilapia and freshwater
bangus. In addition, Batangas became the top 3 which contributes high value due to freshwater

bangus and specially tilapia.

The research of Guerrero, R. III (2018) titled "Farmed Tilapia Production in the Philippines Is

Declining: What Has Happened and What Can Be Done", the Philippine journal science of the

republic of the Philippines stated that there are 300,720 metric tons (MT) and its estimated

valued at ₱24 billion of the total tilapia production in the Philippines which is 86% of the

production come from farms and 14% from inland waters. In 2015, the production of tilapia is

usually coming from freshwater ponds (54%), fresh water cages especially the top 1 province is

Batangas (30%), freshwater pens (8%) and brackish water pond (7%). Most of the productions

are coming from Luzon and Mindanao.

As stated in the article of Aquaculture Research of Sanchez, Vivian‐Rogers, and Urakawa (May

2019) titled" Tilapia reticulating aquaculture systems as a source of plant growth promoting

bacteria", tilapia waste consist of microorganism that helps the plant to balance and maintain its

growth and strong root which become more important than other fertilizer. Microbial people

group decided utilizing high‐throughput sequencing showed the value of Bacteroidetes and

Alphaproteobacteria as advantageous microbial pointers to survey the wellbeing state of

recycling aquaculture frameworks. Siderophore creation, smelling salts generation and

phosphate solubilization measures were utilized for screening and 41% of separates were

distinguished as plant development advancing microbes. It was found that the 32% of bacteria

that is present in tilapia is "Actinobacteria", phylum of Gram-positive microscopic organisms.

They can be earthly or sea-going. They are of extraordinary monetary significance to people

since farming and backwoods rely upon their commitments to the system of soil. The

researchers concluded that the tilapia‐rearing water normally contained different ancestries of

PGPB and could be regarded as a commendable seed bank of PGPB. Since aquaponics is a
troublesome framework to utilize pesticides and herbicides, the job of PGPB to anticipate plant

pathogens and keep up solid root.

Based on the research of Jader Galba Busato, Caroline Moreira de Carvalho, Daniel Basilio

Zandonadi, Fernando Fabriz Sodré, Alan Ribeiro Mol, Aline Limade Oliveira and Rodrigo Diana

Navarro(November 23, 2017) titled “Recycling of wastes from fish beneficiation by composting:

chemical characteristics of the compost and efficiency of their humic acids in stimulating the

growth of lettuce”, A compost made of waste of fishes and crushed grass in using the humic

acid found in the compost in affecting the growth of lettuce by stimulating the compound located

in the soil.

According to Michael James Van Oosten, Olimpia Pepe, Stefania De Pascale, Silvia Silletti and

Albino Maggio (April 3, 2017) titled as” The role of biostimulants and bioeffectors as alleviators

of abiotic stress in crop plants”, The use of bioeffectors, formally known as plant biostimulants,

has become common practice in agriculture and provides a number of benefits in stimulating

growth and protecting against stress. Biostimulants have an effect on the prevention of abiotic

stress of the plant.

According to the research of Ivana Koleška, Dino Hasanagić, Vida Todorović, Senad Murtić,

Izudin Klokić, Nada Parađiković and Biljana Kukavica (May 2, 2017) titled” Biostimulant

prevents yield loss and reduces oxidative damage in tomato plants grown on reduced NPK

nutrition”, it states that biostimulants can prevent degradation of the quality and reduce the

stress genertion of the plant.

According to Chris Sworder (April 9, 2018) “Biostimulants are the nexus of biotechnology and

fertilizer” it states that agricultural industries are using Biostimulants as a nutrient enhancing

substance in increasing the nutrients in the soil for the plant’s benefit. Biostimulants are widely
used in agriculture because it keeps the plants healthy depending on the environment where the

biostimulant is specialized is.

Based on the study Andrea Ertani, Paolo Sambo, Carlo Nicoletto, Silvia Santagata, Michela

Schiavon &Serenella Nardi (April 24, 2015) titled “The use of Biostimulants in hot pepper plants

to help low input sustainable agriculture”, The findings obtained in the present investigation

demonstrate the effectiveness of RG and AH in improving growth and the nutritional value of

peppers. Specifically, the two biostimulants increased the phenol concentration, antioxidant

activity, and ascorbic acid concentration in fruits, as well as the capsaicin concentration in

plants. In the light of these results, the application of biostimulants could be considered as a

good strategy for obtaining high yields of nutritionally valuable vegetables with lower

environmental impact.

In the article of Rachel Backer, J. Stefan Rokem, [...], and Donald L. Smith (Oct. 23, 2018) titled

,” Plant Growth-Promoting Rhizobacteria: Context, Mechanisms of Action, and Roadmap to

Commercialization of Biostimulants for Sustainable Agriculture”, Microbes of the

rhizomicrobiome play key roles in nutrient acquisition and assimilation, improved soil texture,

secreting, and modulating extracellular molecules such as hormones, secondary metabolites,

antibiotics, and various signal compounds, all leading to enhancement of plant growth. The

microbes and compounds they secrete constitute valuable biostimulants and play pivotal roles

in modulating plant stress responses has demonstrated that inoculating plants with plant-

growth promoting rhizobacteria (PGPR) or treating plants with microbe-to-plant signal

compounds can be an effective strategy to stimulate crop growth. Furthermore, these strategies

can improve crop tolerance for the abiotic stresses (e.g., drought, heat, and salinity) likely to

become more frequent as climate change conditions continue to develop.

Nelson, A. &Pagliari, P. (December 5, 2018) affirmed in an article that biostimulants are

separated into 4 types, this are Humic and fulvic acids (plant, animal, and microbial residues),

Seaweed Extracts (can be obtained through extraction processes), Liquid Manure Composting

(made by mixing manure water and other particles needed for microbial growth), and Beneficial

Bacteria and Fungi (it is from concentration of bacteria and/or fungi in the soil that help with root

growth).

Ali, I. (2018) conducted an experiment on applying Biostimulants to a Bell Pepper. The

Biostimulant is made from various chemicals which are mixed in and hand-sprayed every week.

The biostimulant composes of different amounts of chitosan, putrescine, and salycic acid. An

even amount of calcium chloride is also mixed with the chemical. The study was deemed

successful as Irfan Ali (2018) concluded that the bell pepper plants that were sprayed with the

biostimulant produced significant improvements than those of the controlled set-up.

Lugtenburg, B. &Kamilova, F. (2009) stated in the article that the bacteria "Rhizobacteria"

promotes the growth of the plant indirectly or directly. Rhizobacteria can affect the plant's

growth indirectly by strengthening its immune system, and improves the nutrient intake of a

plant. It could also improve the plant's ability to handle abiotic and biotic stress, enhance root

growth and the water intake directly.

An article made by greeneden.co (September 5, 2018) stated that according to he U.S. farm bill

defined biostimulant as "A substance or microorganism that, when applied to seeds, plants, or

the rhizosphere, stimulates natural processes to enhance or benefit nutrient uptake, nutrient

efficiency, tolerance to abiotic stress, or crop quality and yield.” The article also contained the

advantages of using a biostimulant which are increased nutrient and water uptake, increased

root and plant growth, tolerance to Abiotic Stress (Extreme Temperatures, Drought, Flood, High
Winds, Nutrient Deficiency, and Soil Compaction), improved Soil Health, Function, and

Performance, and increased Resistance to Pathogens.

Plants benefit from Rhizobacterias because of the process of the conversion of the Rhizobia of

nitrogen to ammonia, the ammonia is then absorbed by the soil which in turn enriches it and

thus, the plant benefits from it. On the other hand, microorganisms like the one mentioned

earlier benefit due to the nitrogen intake as a source of food and energy as mentioned by

Dardanelli, M.S. ET. al. (September 5, 2010).

A study conducted by Parađiković, N. ET. al. (May 2, 2011) is about the effect of putting

Biostimulants made up of diphenyl, picryl, hydrazyl, and azinobis to pepper cultivars. The study

was deemed to be successful as the pepper plants showed improvements in nutrient uptake

and immune system.

G. Definition of Terms

1. Abating - Become less intense or widespread (subside).

2. Abiotic Stress - Negative impact of non-living factors on the living organisms in a specific

environment.

3. Actinobacteria - Bacteria present in Tilapia.

4. Alphaproteobacteria - They frequently adopt an intracellular lifestyle as plant mutualists or

plant or animal pathogens.

5. Ammonia - A colorless gas with a distinct odor composed of nitrogen and hydrogen atoms. It

is produced naturally in the human body and in nature—in water, soil and air, even in tiny

bacteria molecules.

6. Bacteroidetes - Gram-negative bacteria that ferment polysaccharides and otherwise

indigestible carbohydrates and produce short-chain fatty acids

7. Biomass - Organic matter used as a fuel.

8. Biostimulants - Organic matter used as a fuel, especially in a power station for the

generation of electricity.

9. Biotic Stress - Negative impact of living factors on the living organisms in a specific

environment.

10. Crop Vigor - The capacity for natural growth and survival, as of plants.

11. Endemic - Native and restricted to a certain place.

12. Microorganisms - A microscopic organism, especially a bacterium, virus, or fungus.

13. Pathogen Resistance - Relative to a susceptible plant, disease resistance is the reduction

of pathogen growth on or in the plant (and hence a reduction of disease), while the term disease

tolerance describes plants that exhibit little disease damage despite substantial pathogen levels.

14. PGPB - Plant Growth Promoting Bacteria.

15. Plant-Derived Protein Hydrolysates - Has the potential to improve yield, nutritional quality

as well as tolerance to abiotic stressors.

16. Profluent - Flowing smoothly.

17. Rhizobacteria - Are root-associated bacteria that form symbiotic relationships with many

plants. It is the bacteria that plants benefit from thru converting nitrogen to ammonia.

18. Rhizobia - Rhizobia require a plant host; they cannot independently fix nitrogen. In general,

they are gram negative, motile, non-sporulating rods

19. Rhizosphere - Narrow region of soil that is directly influenced by root secretions, and

associated soil microorganisms known as the root microbiome.

20. Root Emission - the production and growth of a plant's roots.

21. Soil Fertility - The ability of the soil to sustain agriculture plant growth.

22. Solubilization - The process of incorporating the solubilizate (the component that

undergoes solublization) into or onto micelles. Solublization may occur in a system consisting of

a solvent, an association colloid (a colloid that forms micelles), and at least one other

solubilizate.

23. Tabulation - Displaying data in compact form (Table).

24. Translocation - A phenomenon that results in unusual rearrangement of chromosomes.

This includes balanced and unbalanced translocation, with two main types: reciprocal-, and

Robertsonian translocation.

26. Waste Streams - A waste stream is the complete flow of waste from domestic or industrial

areas through to final disposal. The intervention of recycling may act to lessen the content of a

waste stream as it moves down the line.

Chapter 2: Methodology

A. Research Design

This study will have a quantitative research design because it will use numerical data and

tabulation that will consists of 4 (four) tables, the first 3 (three) tables contain the physical

attributes height measured in centimeters (cm),color and odor respectively while the 4th

table contains the summary of all the data collected in the three (3) tables and the study is

also a quasi-experimental research design because the study is caused by the production of

chili in Batangas and the effect is through the biostimulant that can affect the chili plant’s

physical attributes specifically the color, odor and height that can help in production.
B. Population and Sample

The participants of this study are the chili plants and its physical attributes (height, odor and

color) in which will be observe by the researchers. There will be 4 set ups of chili plants. The

controlled set up which is Plant A will have commercialized fertilizer, this is the set up where

the researchers will base if the biostimulant from leftover waste of tilapia has a difference to

the commercialized fertilizer and the set up B, 10g of organic biostimulant will be apply while

for set up C, 15g of organic biostimulant. Lastly, for set up D, 20g of organic biostimulant will

be place in the chili plant. For every set ups, the researchers will observe and collect data

from the chili plant's height in centimeters (cm), color and odor through observational

checklist.
 C. Instrument

The instrument that the researchers will use is the observation checklist that consists of the

plants to be observed with the corresponding amount of biostimulant, the number of days of

observation and the physical attributes to be observed specifically odor, color and height.

Table 1: Height of the Chili Plant

Plants Day 1 Day 3 Day 6 Day 9 Day 12 Day 15

Plant A

(Commercial

Fertilizer)

Plant B

(10 g)

Plant C

(15 g)

Plant D

(20 g)

Average

Table 2: Color of the Chili Plant

Plants Day 1 Day 3 Day 6 Day 9 Day 12 Day 15

Plant A

(Commercial

Fertilizer
 Plant B

(10 g)

Plant C

(15 g)

Plant D

(20 g)

Table 3: Odor of the Chili Plant

Plants Day 1 Day 3 Day 6 Day 9 Day 12 Daty15

Plant A

(Commercial

Fertilizer)

Plant B

(10 g)

Plant C

(15 g)

Plant C

(20 g)

Table 4: Summarization of the Data Collected

Plants Day 1 Day 3 Day 6 Day 9 Day 12 Day 15

Plant A H O C H O C H O C H O C H O C H O C
(Commercial

Fertilizer)

Plant B H O C H O C H O C H O C H O C H O C

(10 g)

Plant C H O C H O C H O C H O C H O C H O C

(15 g)

Plant D H O C H O C H O C H O C H O C H O C

(20 g)
D. Data Collection

The researchers would use observational techniques in observing any changes among the

chili plants of the research and will also use experimental techniques where the researchers

would set up a variety of test samples such as chili plants in the experiment when given with

different variables such as the biostimulants and record any changes in its physical

attributes specifically the height, odor and color of the chili plant.
 E. Data Analysis

For this study, the researchers will be using three data analysis techniques: tabulation,

observation and coding. First is the Tabulation technique for recording the data that will be

collected by the researchers that is divided into three (3): the height, color, and odor of the chili

plant and Plant A will be the controlled set-up that will not receive any amount of biostimulant

and fourth table for summary of the data collected in the three (3) tables. Second is the

Observation technique that the researchers will use in order to analyze the changes happening

in the chili plants and lastly is the Coding technique that the researchers will use codes to

identify the physical attributes color and odor of the chili plant.

For Table 1 which is the table for the height of the chili plant in which the researchers will

measure the plant by centimeters and data will be analyzed through observation. The plants will

be measured every 3 days for 15 days to effectively see the difference of the plants with

different amounts of the biostimulant mixed in specifically 10 grams, 15 grams, and 20 grams

respectively. The equation for getting the average or the mean is to be used.

∑𝑥
Mean is equals to where x is the height collected and N is the number of data collected.
𝑁

For Tables 2 and 3 which is the table of color and odor respectively where in the researchers

will use the coding technique for easier recording and analysis. For the color, the codes R for

Red, G for Green, RG for Reddish Green, and Y for Yellow are going to be used by the

researchers to identify the color produced by the different amount of biostimulant in the four

plants while for the odor, the codes F for fragrant, S for Smelly and Od for odorless are going to

be used by the researchers to identify the smell produced by the plants.

For table 4 which is the table containing the summarization of the first three (3) tables that will

help the researchers visualize the overall result of the data collected and it can also help in

easier interpretation.