SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

Group 2 – SAMPLE QUESTIONS AND SOLUTIONS

AN OVERVIEW OF BIOLOGICAL BASICS
Compiled by: Decatoria, Terry Clarice SJ.
Garcia, Dale Tristan O.
Lazaro, Janielle Xandra
Legaspi, Lhaila T.
SAMPLE QUESTIONS:
1. In RNA, instead of Thymine, _____ is used.
(A) Guanine (C) Cytosine
(B) Uracil (D) Adenine

2. This is a cell that grow best at low temperature (below 2℃)

(A) Lipophiles (C) Thermophiles
(B) Mesophiles (D) Psychrophiles

3. Viruses cannot capture or store free energy and are functionally active except when inside
their host cells.
(A) True (C) Sometimes true
(B) False (D) Cannot be determined

4. These are bacteria that can grow in an environment with only a little moisture and few
dissolved minerals
(A) Faculative Bacteria (C) Cyanobacteria
(B) E.coli (D) Bacillus

5. Which of the following statements is true about the nomenclature of E.coli?

(A) Escherichia is the genus and coli is the species
(B) Coli is the genus and Escherichia is the species
(C) Espergillus is the genus and coli is the species
(D) Coli is the genus and Espergillus is the species

6. In eukaryotes, the cell nucleus contains nucleic acids in the form of

(A) RNA (C) mRNA
(B) DNA (D) Enzymes

7. I. The cellular macromolecules are functional only when in the proper two-dimensional-
configuration.
II. The interaction among them is very complicated.
(A) Only the first statement is true
(B) Only the second statement is true
(C) Both statements are true
(D) Both statements are false

COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.

LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.
SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

8. Most of the enzymes and metabolic intermediates are present in the_____.

(A) Nucleus (C) Endoplasmic Reticulum
(B) Cell Membrane (D) Cytoplasm

9. I. Living cells are composed of high-molecular-weight monomeric compound such as proteins

and nucleic acids.
II. These biopolymers constitute the major structural elements of the living cell.
(A) Only the first statement is true
(B) Only the second statement is true
(C) Both statements are true
(D) Both statements are false

10. These are very small and obligate parasites if other cells.
(A) Bacteria (C) Virus
(B) Influenza (D) Protists

11. It is the most abundant organic molecules in living cells which constitutes 40% to 70% of
their dry weight.
(A) Carbohydrates (C) Lipids
(B) Proteins (D) Nucleic Acids

12. The amino acids that are considered the building blocks of proteins and contain at least one
carboxyl group
(A) α-amino acids (C) γ-amino acids
(B) β-amino acids (D) θ-amino acids

13. Each enzyme’s molecule contains a/an _____ to which its specific substrate is bound during
catalysis.
(A) location site (C) web site
(B) specific site (D) active site

14. It refers to larger amino acids.

(A) Carbohydrates (C) Lipids
(B) Proteins (D) Nucleic Acids

15. The sizes of most prokaryotes vary from 0.5 to 3 _____ in equivalent radius.
(A) Micrometer (C) Meter
(B) Nanometer (D) Millimeter

16. What is the shape of the species Rhodospirillum?

(A) Spiral (C) Cylindrical
(B) Spherical (D) NOTA

COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.

LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.
SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

17. What is the shape of the species Staphylococci?

(A) Spiral (C) Cylindrical
(B) Spherical (D) NOTA

18. Aroxygenic photosynthetic bacteria (the purple and green bacteria) have light-gathering
pigments called
(A) Streptomyces (C) Bacteriochlorophyll
(B) Thermomonospora (D) Actinomyces

19. _____ are five times larger than _____ in diameter.

(A) Prokaryotes; Eukaroytes
(B) Eukaroytes; Prokaryotes
(C) Eubacteria; Probacteria
(D) Probacteria; Eubacteria

20. It strengthens the structure and make the membrane less flexible
(A) Sterols (C) Eukaryotes
(B) Chromosomes (D) Prokaryotes

21. Which of the following does not belong in the eukaryotes group?
(A) Protists (C) Animals
(B) Plants (D) Bacteria

22. In the replication of a virulent bacteriophage, on what stage does the phage DNA directs the
cell’s metabolism to produce viral components?
(A) Adsorption (C) Biosynthesis
(B) Maturation (D) Penetration

23. What is the cycle wherein phage DNA may be incorporated into the host DNA, and the host
may continue to multiply on this state?
(A) Lysonic stage (C) Lysogenic stage
(B) Lytic stage (D) Linolic stage

24. The following are included in an animal cell except

(A) Cell wall (C) Chromosomes
(B) Cytoplasmic membrane (D) Nucleus

25. How many phases does the cell-division cycle has?

(A) 2 (C) 4
(B) 3 (D) 5

COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.

LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.
SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

26. Among the following are the diverse biological functions of proteins except
(A) Structural protein (C) Process protein
(B) Regulatory protein (D) Transport protein

27. An amino acid that has positively and negatively charged groups, a dipolar molecule is called
(A) Bi-charged ion (C) Dual-charged ion
(B) Twitterion (D) Zwitterion

28. The condensation reaction between two amino acids results in the formation of
(A) Covalent bond (C) Ionic bond
(B) Peptide bond (D) Hydrogen bond

29. A macromolecule which key role involves structural and storage compounds in cells.
(A) Carbohydrates (C) Lipids
(B) Proteins (D) Nucleic Acids

30. Carbohydrates are represented in the formula (CH2O)n, where n is

(A) n ≥ 1 (C) n ≥ 3
(B) n ≥ 2 (D) n ≥ 4

31. Monosaccharides are in the form of this/these organic compounds

(A) Aldehydes (C) Both A and B
(B) Ketones (D) NOTA

32. What type of bond was utilized when polysaccharides are formed by the condensation of
more than two monosaccharides?
(A) Peptide Bond (C) Ionic Bond
(B) Covalent Bond (D) Glycosidic Bond

33. It is the sugar molecule in the nucleotide.

(A) Tetrose (C) Triose
(B) Hexose (D) Pentose

34. Which among the following is not classified as a pyrimidine?

(A) Uracil (C) Cytosine
(B) Adenine (D) Thymine

35. In RNA or DNA, Guanine is always pair with

(A) Uracil (C) Cytosine
(B) Adenine (D) Thymine

COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.

LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.
SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

36. Which of the following statements is true regarding transcription and translation?
(A) During transcription, an mRNA (messenger RNA) molecule is created from the DNA
molecule.
(B) Translation takes place in the nucleus, while transcription takes place in the
cytoplasm.
(C) During protein synthesis, translation occurs prior to transcription.
(D) During translation, a DNA sequence is used to synthesize an amino acid sequence.

37. Which functional group is present in phospholipid?

(A) Carbonyl (C) Amino
(B) Hydroxyl (D) Phosphate

38. Butter and animal fats are an example of the following

(A) Cholesterol (C) Unsaturated fats
(B) Trans fats (D) Saturated fats

39. The following are examples of macronutrients except for?

(A) Calcium (C) Magnesium
(B) Potassium (D) Phosphorus

40. How much nucleic acid does a cell contain in terms of dry basis?
(A) 5-15% (C) 10-20%
(B) 5-20% (D) about 90%

41. Which macronutrient is a cofactor for some enzymes and is required in carbohydrate
metabolism?
(A) Calcium (C) Magnesium
(B) Potassium (D) Phosphorus

42. What macronutrient is required as a terminal electron acceptor in the aerobic metabolism of
carbon compounds?
(A) Excess oxygen (C) Free oxygen
(B) Gaseous oxygen (D) Molecular oxygen

43. Which microorganism utilize CO2 as a carbon source and obtain energy from the oxidation
of inorganic compounds?
(A) Heterotrophs (C) Mixotrophs
(B) Chemoautotrophs (D) Photoautotrophs

44. The following are common carbon sources except for?

(A) Glucose (C) Lactose
(B) Fructose (D) Sucrose

45. It is the constituent of nucleic acids, phospholipids, nucleotides, and certain coenzymes.
(A) Carbon (C) Phosphorus
(B) Nitrogen (D) Potassium
COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.
LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.
 SAMPLE QUESTIONS AND SOLUTIONS BIOCHEMICAL ENGINEERING

46. _____________ is also an enzyme cofactor and plays a role in the regulation of secondary
metabolism and excretion of primary metabolites.
(A) Manganese (C) Carbon
(B) Zinc (D)Magnesium

47. ______________ is needed in trace amounts by some bacteria, especially by methanogens

for ion balance.
(A) Chlorine (C) Sodium
(B) Nickel (D) Selenium

48. __________ contain specific amounts of pure chemical compounds with known chemical
compositions.
(A) Complex Media (C) Undefined Media
(B) Defined Media (D) Mass Media

49. Which of the following is not a Defined Medium?

(A) Glucose (C) ZnSO4◊7H2O
(B) KH2PO4 (D)Corn Steep Liquor

50. ___________ contain natural compounds whose chemical composition is not exactly known.
(A) Complex Media (C) Undefined Media
(B) Defined Media (D) Mass Media

ANSWER FOR QUESTIONS 1-50:

Number Answer Number Answer Number Answer Number Answer Number Answer
1 B 11 B 21 D 31 C 41 B
2 D 12 A 22 C 32 D 42 D
3 B 13 D 23 C 33 D 43 B
4 C 14 B 24 A 34 B 44 C
5 A 15 A 25 C 35 C 45 C
6 B 16 A 26 C 36 A 46 A
7 B 17 B 27 D 37 D 47 C
8 D 18 C 28 B 38 D 48 B
9 B 19 B 29 A 39 A 49 D
10 C 20 A 30 C 40 C 50 A

COMPILED BY: DECATORIA, TERRY CLARICE SJ, GARCIA, DALE TRISTAN O.

LAZARO, JANIELLE XANDRA, LEGASPI, LHAILA T.