Nursing Biochemistry

Laboratory Report

Carbohydrates 1: Common
Reaction
Activity No. 3

Sanaani, Nur-Fatima M. (Principal Author)

Lui, Sharah Ghyll P.
Jasim, Nerizza B.
Locker No.33; NurBio Lab C
1st Semester, SY 2019-20
Nursing Biochemistry
Laboratory Report

RATIONALE
This activity on carbohydrates will involve two parts: its common and specific reaction it will entail two-
two laboratory periods.

Considered as one of the main biomolecules, carbohydrates are also considered as the most
abundant class of compounds in the biological world, coincidentally making up more than 50% of the dry
weight of the Earth’s biomass. These biomolecules is basically needs, and important component of all
living organism and have variety of different function. The term “carbohydrate” is derived from the
French “hydrate de carbon” and is a term that is also a synonym of “saccharide”, inclusive of sugars,
starch and cellulose. Both of these terms are actually used interchangeably when referring to them by
modern scientific standards.

Objective:

I. The use of chemical tests to identify some physical and chemical characteristics of typical
carbohydrates
II. Differentiate between monosaccharides, disaccharides and polysaccharides using the tests
included above

DATA INTERPRETATION:
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eu feugiat nulla facilisis at vero eros et accumsan.

Table No. 1

A. Molisch Test
Test Solutions Result Explanation

1% Glucose

1% Xylose

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Nursing Biochemistry
Laboratory Report

1% Lactose

1% Sucrose

1% Starch

1% Furfural

Unknown Sugar

DATA INTERPRETATION:

Table No. 2

B. Action of Alkali (Search for Theoretical Result Only)

Alkali Test Result Explanation

Solution

10% NaOH 1%
Glucose

1%
Fructose

1%
Galactose

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

1% Ba(OH)2 1%
Glucose

1%
Fructose

1%
Galactose

DATA INTERPRETATION:

Table No. 3

C. Reduction Test
Name of Test Test Result Explanation
Solution
1%
Fehling’s Test
glucose
1%
xylose
1%
fructose
1%
lactose

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

1%
maltose
1%
sucrose
1%
starch
1%
glycogen
Unknown
sugar
1%
Benedict’s
glucose
Test
1%
xylose
1%
fructose
1%
lactose
1%
sucrose
1%
starch
1%
Barfoed’s Test
gluctose
1%
fructose
1%
xylose
1%
lactose
1%
maltose
1%
sucrose
Unknown
sugar

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

DATA INTERPRETATION:

Table No.4

Name of Test Test Result Explanation

Solution

Nylander’sn 1%glucose
Test

(Search for 1%fructose

Theoretical
Result Only)

1% xylose

1%lactose

1%
maltose

Picric Acid 1% glucose

Test

1%
fructose

1% xylose

1% lactose

1%maltose

Tollen’s Test 1% glucose

Classification of unknown sugar________________________________________

ANSWERS TO QUESTIONS

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

1. Why do all sugars give a positive result for molisch’s test?

ANSWER:
2. What compound other than α- naphthol can be used as a reagent for the test??

ANSWER:

3. Why is it important to use freshly prepared Fehling’s reagent?

ANSWER:

4. Compare the action of a strong and a weak alkali on a reducing sugar?

ANWERS:
5. How are the conditions for barfoed’s test different from those for Benedict ’s test?
ANSWER:
6. Account for the black precipitate in Nylander’s test?
ANSWERS:

CONCLUSION

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laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation
ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat.

REFERENCES
http://www.namrata.co/category/practical-biochemistry/reactions-of-carbohydrate
• "Laboratory Tests for Cabohydrates"
http://www.slideshare.net/katealyssacaton/seliwanoff-benedicts-test
• "Testing for Cabohydrates"
https://biokamikazi.files.wordpress.com/2013/10/merged_document.pdf
• "Benedict's Test"
www.harpercollege.edu/tm-ps/chm/100/dgodambe/thedisk/carbo/bened/benedict.htm
• "Drawing Sugar Structures"
www.chtf.stuba.sk/~szolcsanyi/education/files/Chemia%20heterocyklickych%20zlucenin/Pred
naska%206/Odporucane%20studijne%20materialy/Drawing%20sugar%20structures.pdf
• "Carbohydrates"
chemed.chem.purdue.edu/genchem/topicreview/bp/1biochem/carbo5.html
• "Amylose"

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University
Nursing Biochemistry
Laboratory Report

en.m.wikipedia.org/wiki/Amylose
• "Benedict's Test"
www.harpercollege.edu/tm-ps/chm/100/dgodambe/thedisk/carbo/bened/benedict.htm
• "Benedict's Reagent"
www2.sunysuffolk.edu/sabatil/by14/by14lab3/benedict.htm
• "Reducing Sugars"
en.m.wikipedia.org/wiki/Reducing_sugar
• "Reducing Sugar"
www.ausetute.com.au/redsugar.html
• "Barfoed's Test"
www.harpercollege.edu/tm-ps/chm/100/dgodambe/thedisk/carbo/barf/barfoed.htm
• "Functional Tests for Cabohydrates"
https://www.msu.edu/course/lbs/145/luckie/Lab1.html
generalchemistrylab.blogspot.in/2011/12/iodine-test-for-starch-and-glycogen.html?m=1

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Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga University