Biochemical oxygen demand (BOD)

2.2 APPARATUS AND MATERIAL

i. Sampling bottles

ii. Measuring cylinder

iii. pH meter

iv. DO meter

v. Distilled water
3.0 METHODOLOGY

3.2 Procedure BOD test

3.2.1 100ml of waste water is collected from a specified source which is lake
in front FKAAS using a bottle.
3.2.2 The waste water is then being tested by using pH meter. The pH should
be in between 6.5-8.2 and the reading was recorded.

3.2.3 The water is then being divided by using measuring cylinder which is
10ml

3.2.4 The sample is poured into first, second and third bottle. The water is the
is fulled with deionized water until 300ml. We used 3 sample to
calculate its average.
3.2.5 The fourth bottle is blank water. We prepared by poured 300ml of
deonized water until its full.

3.2.6 Next, we got the DO reading by using DO meter. Same procedure for the
other bottles.
 3.2.7 After we achieved the DO readings, the bottles were kept in BOD
incubator and remain around 20℃

3.2.8 The final DO is recorded after 5 days of the experiment.

4.0 RESULTS AND CALCULATIONS

Sample:
pH: 7.98
Source: Lake water in front FKAAS

BOD data table

Date: 5 March 2019
Sample of 10ml of waste water
Initial DO (mg/l) Final DO(mg/l)
Blank 8.79 8.74
Sample 8.77 8.76

10𝑚𝑙
Dillution factor, P = = 0.03
300𝑚𝑙

Calculation of BOD

𝐷1−𝐷2
BOD,mg/l =
𝑃

Sample 10ml of waste water

 8.77−8.76
BOD,mg/l =
0.03
=0.33

Blank sample
8.79−8.74
BOD,mg/l =
0.03
=1.67

5.0 DISCUSSION
The expriment result that we obtained is consistence with the theory. BOD will getting higher
if the number of microorganisms increase in the water sample. From the experiment, we can
see that the lower the dillution factor, the higher the BOD.

6.0 CONCLUSION
From the experiment, we can conclude that the water sample is in the class I. It is good good
conservation of natural environment and practically no treatment is necessary.