Germination rate

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
1 1 1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1 1 1

100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
Total 0 29
No of leaves

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
5 0 0 0 0 2 2 0 0 0
4 0 0 0 0 2 2 0 0 0
4 0 0 0 0 0 1 0 0 0

Hormone treatment (mg/L) against root proliferation (cm)

Length of root (cm)

7.5 7.1
7
6.5
6
5.5
5
4.5
Length (cm)

4
3.5
3
2.5
2
1.5
1
0.5 0.2333333333
0
Control 1 20 30 40 50 60 70 80 90
Treatment
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance Average
Control 3 13 4.333333 0.333333 7.1
1 3 0 0 0 0.233333
2 3 0 0 0 0
3 3 0 0 0 0
4 3 0 0 0 0
5 3 4 1.333333 1.333333 0
6 3 5 1.666667 0.333333 0
7 3 0 0 0 0
8 3 0 0 0 0
9 3 0 0 0 0

Hormone tre
ANOVA
5
Source of Variation SS df MS F P-value F crit
Between Groups 53.86667 9 5.985185 29.92593 1.09E-09 2.392814
4.3333333333
Within Groups 4 20 0.2
4
Total 57.86667 29

Number of leaves
2

0
Control 1
0
 Treatment Mean Variance
Control 4.333 ± 0.58a 0.3333
Treatment 1 = 1.0 NAA + 0.1 Kinetin 0.000c 0
Treatment 2 = 0.5 NAA + 0.5 Kinetin 0.000c 0
Treatment 3 = 0.1 NAA + 1.0 Kinetin 0.000c 0
Treatment 4 = 1.0 NAA + 0.1 BAP 0.000c 0
Treatment 5 = 0.5 NAA + 0.5 BAP 1.333 ± 1.15b 1.3333
Treatment 6 = 0.1 NAA + 1.0 BAP 1.667 ± 0.58b 0.3333
Treatment 7 = 1.0 2,4-D + 0.1 BAP 0.000c 0
Treatment 8 = 0.5 2,4-D + 0.5 BAP 0.000c 0
Treatment 9 = 0.1 2,4-D + 1.0 BAP 0.000c 0

Values followed by the same letter within the same column do not differ significantly at p < 0.05 by Duncan Multiple Range

Hormone treatment (mg/L) against number of leaves

5

4.3333333333

3
Number of leaves

2
1.6666666667
1.3333333333

0
Control 1
0 2
0 3
0 4
0 5 6 7
0 8
0 9
0

Treatment
0.05 by Duncan Multiple Range Test (DMRT).
Control T1 T2 T3 T4 T5 T6 T7 T8 T9
1 1 1 0 0 1 1 0 0 1
1 1 1 0 0 1 1 0 0 1
1 1 1 0 0 1 1 0 0 1
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Control 3 3 1 0 100%
Treatment 1 3 3 1 0 100%
Treatment 2 3 3 1 0 33.33%
Treatment 3 3 0 0 0 100%
Treatment 4 3 0 0 0 66.67%
Treatment 5 3 3 1 0 66.67%
Treatment 6 3 3 1 0 66.67%
Treatment 7 3 0 0 0 100%
Treatment 8 3 0 0 0 66.67%
Treatment 9 3 3 1 0 66.67%

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 7.2 9 0.8 65535 #DIV/0! 2.392814
Within Groups 0 20 0

Total 7.2 29
Root length (cm)

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
7.7 0.3 0 0 0 0 0 0 0 0
6.4 0.4 0 0 0 0 0 0 0 0
7.2 0 0 0 0 0 0 0 0 0

Hormone treatment (mg/L) against root length (cm)

8.5

7.5

6.5

5.5

4.5

3.5

2.5

1.5

0.5

0
10
1

9
n

n
n

n
um

um

um

um

um

um

um
um

um

um
l

l
Co

Co

Co

Co

Co

Co

Co

Co

Co

l
Co
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 1 3 21.3 7.1 0.43
Column 2 3 0.7 0.233333 0.043333
Column 3 3 0 0 0
Column 4 3 0 0 0
Column 5 3 0 0 0
Column 6 3 0 0 0
Column 7 3 0 0 0
Column 8 3 0 0 0
Column 9 3 0 0 0
Column 10 3 0 0 0

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 135.26 9 15.02889 317.5117 1.41E-19 2.392814
Within Groups 0.946667 20 0.047333

Total 136.2067 29
Treatment Mean Variance
Control 6.9000 ± 0.98a 0.97
Treatment 1 = 1.0 NAA + 0.1 Kinetin 0.000b 0
Treatment 2 = 0.5 NAA + 0.5 Kinetin 0.000b 0
Treatment 3 = 0.1 NAA + 1.0 Kinetin 0.000b 0
Treatment 4 = 1.0 NAA + 0.1 BAP 0.000b 0
Treatment 5 = 0.5 NAA + 0.5 BAP 0.000b 0
Treatment 6 = 0.1 NAA + 1.0 BAP 0.000b 0
Treatment 7 = 1.0 2,4-D + 0.1 BAP 0.000b 0
Treatment 8 = 0.5 2,4-D + 0.5 BAP 0.000b 0
Treatment 9 = 0.1 2,4-D + 1.0 BAP 0.000b 0
Values followed by the same letter within the same column do not differ significantly at p < 0.05 by Duncan Multiple Range Tes
ncan Multiple Range Test (DMRT).
Control T1 T2 T3 T4 T5 T6 T7 T8 T9
0 0 0 1 1 0 0 1 1 0
0 0 0 1 1 0 0 1 1 0
0 0 0 1 0 0 0 1 0 0

No. of s hoot No. of cal l us

1 1 1 1 1 1 1 1
1

0.8
0.6666666667 0.6666666667
Percentage (%)

0.6

0.4

0.2

0
Control
0 10 20 03 04 50 60 07 08 90
Hormone treatment
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance Average
Control 3 0 0 0 1 1
1 3 0 0 0 1 1
2 3 0 0 0 1 0.33
3 3 3 1 0 0 1
4 3 2 0.666667 0.333333 0 0.6667
5 3 0 0 0 1 0.6667
6 3 0 0 0 1 0.6667
7 3 3 1 0 0 1
8 3 2 0.666667 0.333333 0 0.6667
9 3 0 0 0 1 0.6667

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 5.333333 9 0.592593 8.888889 2.78E-05 2.392814
Within Groups 1.333333 20 0.066667

Total 6.666667 29
Callus Fresh Weight (g)

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
0 0 0 0.2233 0.6029 0 0 2.33 1.1975 0
0 0 0 0.1305 0.3685 0 0 0.9248 0.6739 0
0 0 0 0.1251 0 0 0 0.2943 0 0

Hormone treatment (mg/L) against diameter of callus (cm)

2
1.8

1.6
1.4

1.2333333333
1.2
Axis Title

0.8666666667

0.8

0.4

0 0
Control 10 20 3 4 50 60 7 8 90
Axis Title
 Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance Average
Control 3 0 0 0 0
1 3 0 0 0 0
2 3 0 0 0 0
callus (cm) 3 3 0.4789 0.159633 0.003047 0.866667
4 3 0.9714 0.3238 0.092371 1.233333
5 3 0 0 0 0
8 6 3 0 0 0 0
7 3 3.5491 1.183033 1.086032 1.8
8 3 1.8714 0.6238 0.360384 1.4
9 3 0 0 0 0
1.4

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 4.183474 9 0.46483 3.014789 0.019088 2.392814
Within Groups 3.083668 20 0.154183

Total 7.267142 29

8 90
Treatment Mean Variance
Control 0.000b 0
Treatment 1 = 1.0 NAA + 0.1 Kinetin 0.000b 0
Treatment 2 = 0.5 NAA + 0.5 Kinetin 0.000b 0
Treatment 3 = 0.1 NAA + 1.0 Kinetin 0.1596 ± 0.06b 0.003
Treatment 4 = 1.0 NAA + 0.1 BAP 0.3238 ± 0.30b 0.0924
Treatment 5 = 0.5 NAA + 0.5 BAP 0.000b 0
Treatment 6 = 0.1 NAA + 1.0 BAP 0.000b 0
Treatment 7 = 1.0 2,4-D + 0.1 BAP 1.1830 ± 1.04a 1.086
Treatment 8 = 0.5 2,4-D + 0.5 BAP 0.6238 ± 0.60ab 0.3604
Treatment 9 = 0.1 2,4-D + 1.0 BAP 0.000b 0
Values followed by the same letter within the same column do not differ significantly at p < 0.05 by Duncan Multiple Range

Hormone treatment (mg/L) against fresh weight of callus (g)

1.4 1.183033333
3
1.2
Fresh weight (g)

1
0.8
0.6238
0.6
0.4 0.3238
0.159633333
0.2 3
0
Control
0 1
0 2
0 3 4 5
0 6
0 7 8 9
0
Treatment
0.05 by Duncan Multiple Range Test (DMRT).
Diameter callus (cm)

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
0 0 0 0.5 2.3 0 0 2.5 2.3 0
0 0 0 1.2 1.4 0 0 1.8 1.9 0
0 0 0 0.9 0 0 0 1.1 0 0

1.8

1.6

1.4

1.2

0.8

0.6

0.4

0.2

0
l
tro t1 t2 t3 t4 t5 t6 t7 t8 t9
n en en en en en en en en en
Co tm tm tm tm tm tm tm tm tm
a a a a a a a a a
Tre Tre Tre Tre Tre Tre Tre Tre Tre
Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Control 3 0 0 0
Treatment 1 3 0 0 0
Treatment 2 3 0 0 0
Treatment 3 3 2.6 0.866667 0.123333
Treatment 4 3 3.7 1.233333 1.343333
Treatment 5 3 0 0 0
Treatment 6 3 0 0 0
Treatment 7 3 5.4 1.8 0.49
Treatment 8 3 4.2 1.4 1.51
Treatment 9 3 0 0 0

ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 13.98967 9 1.554407 4.483868 0.002508 2.392814
Within Groups 6.933333 20 0.346667

Total 20.923 29
Treatment Mean Standard deviation
Control 0.000b 0
Treatment 1 = 1.0 NAA + 0.1 Kinetin 0.000b 0
Treatment 2 = 0.5 NAA + 0.5 Kinetin 0.000b 0
Treatment 3 = 0.1 NAA + 1.0 Kinetin 0.867 ± 0.35ab 0.1233
Treatment 4 = 1.0 NAA + 0.1 BAP 1.233 ± 1.16a 1.3433
Treatment 5 = 0.5 NAA + 0.5 BAP 0.000b 0
Treatment 6 = 0.1 NAA + 1.0 BAP 0.000b 0
Treatment 7 = 1.0 2,4-D + 0.1 BAP 1.800 ± 0.70a 0.49
Treatment 8 = 0.5 2,4-D + 0.5 BAP 1.400 ± 1.23a 1.51
Treatment 9 = 0.1 2,4-D + 1.0 BAP 0.000b 0
Values followed by the same letter within the same column do not differ significantly at p < 0.05 by Duncan Multiple Range Tes
Duncan Multiple Range Test (DMRT).
Percentage survival

Control T1 T2 T3 T4 T5 T6 T7 T8 T9
1 1 1 1 1 1 1 1 1 1
1 1 0 1 1 1 1 1 1 1
1 1 0 1 0 0 0 1 0 0

100% 100% 33.33% 100% 66.67% 66.67% 66.67% 100% 66.67% 66.67%

100% 73% 17% 100% 57% 29% 22% 86% 57% 66.67%

Percentage of survival (%)

100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
l
tro t1 t2 t3 t4 t5 t6 t7 t8 t9
n en en en en en en en en en
Co tm tm tm tm tm tm tm tm tm
a a a a a a a a a
Tre Tre Tre Tre Tre Tre Tre Tre Tre
Control 100%
Treatment 1 33.33%
Treatment 2 100%
Treatment 3 66.67%
Treatment 4 66.67%
Treatment 5 66.67%
Treatment 6 100%
Treatment 7 66.67%
Treatment 8 66.67%
Treatment 9 100%

Treatment Percentage of germination

Control 100%
Treatment 1 = 1.0 NAA + 0.1 Kinetin 100%
Treatment 2 = 0.5 NAA + 0.5 Kinetin 100%
Treatment 3 = 0.1 NAA + 1.0 Kinetin 100%
Treatment 4 = 1.0 NAA + 0.1 BAP 100%
Treatment 5 = 0.5 NAA + 0.5 BAP 100%
Treatment 6 = 0.1 NAA + 1.0 BAP 100%
Treatment 7 = 1.0 2,4-D + 0.1 BAP 100%
Treatment 8 = 0.5 2,4-D + 0.5 BAP 100%
Treatment 9 = 0.1 2,4-D + 1.0 BAP 100%
Percentage of survival
100%
100%
33.33%
100%
66.67%
66.67%
66.67%
100%
66.67%
66.67%
Observation

Observation
Type of callus Compact, friable
Colour of callus White creamish and white greenish, purple pigmentation in T3