Académique Documents
Professionnel Documents
Culture Documents
Peristaltic pump
Syringe pump
Separations in FIA
D = 1 / (1 – e-VK)
V: sample volume
K: a constant related to the experimental configuration
65%
35%
Stopped Flow Method Using FIA
LDH
Pyruvate + NADH + H+ Lactate + NAD+
The dispersed zone (top left) contains two equivalence points (A and B),
the distance between them, Dt, being proportional to the acid
concentration in the injected sample zone.
All FIA titrations are based on peak-width (Dt) measurement.
Flow Injection Titration of HCl with 1 mM NaOH
molarity of HCl
The time interval between the points is a measure of the acid concentration
Merging Zone FIA
pump
sample
Carrier fluid
reagent