3

Journal of Environmental Sciences Volume 26 Number 11 2014

JOURNAL OF ENVIRONMENTAL SCIENCES

www.jesc.ac.cn

2139 Review of the progress in preparing nano TiO2: An important environmental engineering material
Yan Wang, Yiming He, Qinghua Lai, and Maohong Fan
2178 Utilization of plant-based natural coagulants as future alternatives towards sustainable water clarification
Sook Yan Choy, Krishna Murthy Nagendra Prasad, Ta Yeong Wu, Mavinakere Eshwaraiah Raghunandan,
and Ramakrishnan Nagasundara Ramanan
2190 Effect of different molecular weight organic components on the increase of microbial growth
potential of secondary effluent by ozonation
Xin Zhao, Hong-Ying Hu, Tong Yu, Chang Su, Haochi Jiang, and Shuming Liu

2198 Perfluoroalkyl substances and organochlorine pesticides in sediments from Huaihe watershed in China
Jing Meng, Tieyu Wang, Pei Wang, John P. Giesy, and Yonglong Lu
2207 Effects of pH on photochemical decomposition of perfluorooctanoic acid in different atmospheres by 185
nm vacuum ultraviolet
Yuan Wang and Pengyi Zhang
2215 Aerobic granules cultivated and operated in continuous-flow bioreactor under particle-size
selective pressure
Hongbo Liu, Hang Xiao, Shuai Huang, Huijun Ma, and He Liu

2222 Attenuation of arsenic in a karst subterranean stream and correlation with geochemical factors:
A case study at Lihu, South China

VOLUME 26
Liankai Zhang, Hui Yang, Jiansheng Tang, Xiaoqun Qin, and Au Yik Yu

2231 Nitrogen removal from coal gasification wastewater by activated carbon technologies
combined with short-cut nitrogen removal process

NUMBER 11
Qian Zhao, Hongjun Han, Baolin Hou, Haifeng Zhuang, Shengyong Jia, and Fang Fang

2240 Evaluating the emission status of light-duty gasoline vehicles and motorcycles in Macao with real-
world remote sensing measurement
Yu Zhou, Ye Wu, Shaojun Zhang, Lixin Fu, and Jiming Hao

Pages 2139–2368
2249 Experimental study of NO2 reduction in N2/Ar and O2/Ar mixtures by pulsed corona discharge
Xinbo Zhu, Chenghang Zheng, Xiang Gao, Xu Shen, Zhihua Wang, Zhongyang Luo, and Kefa Cen

2257 Absorption characteristics of elemental mercury in mercury chloride solutions

Yongpeng Ma, Haomiao Xu, Zan Qu, Naiqiang Yan, and Wenhua Wang

2014
2266 Variations in stable hydrogen and oxygen isotopes in atmospheric water vapor in the marine
boundary layer across a wide latitude range
Jingfeng Liu, Cunde Xiao, Minghu Ding, and Jiawen Ren
2277 Effect of ammonium sulfate and urea on PCDD/F formation from active carbon and possible
mechanism of inhibition
Mi Yan, Zhifu Qi, Jie Yang, Xiaodong Li, Jianli Ren, and Zhang Xu

2283 Theoretical study on the degradation reaction of octachlorinated dibenzo-p -dioxin with atomic
oxygen O(3P) in dielectric barrier discharge reactor
Chen Gong, Xiaomin Sun, Chenxi Zhang, Jingtian Hu, and Chuansong Qi

2290 An assessment of the effectiveness and impact of electrokinetic remediation for

pyrene-contaminated soil
Sujuan Xu, Shuhai Guo, Bo Wu, Fengmei Li, and Tingting Li
ELSEVIER
 CONTENTS

Editorial Board of Journal of Environmental Sciences

Editor-in-Chief
Hongxiao Tang Chinese Academy of Sciences, China, E-mail: tanghx@rcees.ac.cn

Associate Editors-in-Chief
2298 Effect of surfactant on phenanthrene metabolic kinetics by Citrobacter sp. SA01
Feng Li, Lizhong Zhu, and Dong Zhang
Nigel Bell Imperial College London, UK, E-mail: n.bell@imperial.ac.uk
Shu Tao Peking University, China, E-mail: taos@urban.pku.edu.cn 2307 Cytochrome P450 BM3 of Bacillus megaterium — A possible endosulfan biotransforming gene
Jiuhui Qu Chinese Academy of Sciences, China, E-mail: jhqu@rcees.ac.cn Muhil Vannan Seralathan, Saravanadevi Sivanesan, Amit Bafana,
P.K. Wong The Chinese University of Hong Kong, Hong Kong, China, E-mail: pkwong@cuhk.edu.hk Sanjay Madanchand Kashyap, Arrigo Patrizio, Kannan Krishnamurthi, and
Tapan Chakrabarti1
Editorial Board
Aquatic environment Michael Schloter Environmental toxicology and health 2315 Phosphatase activity and culture conditions of the yeast Candida mycoderma sp. and analysis of
Baoyu Gao German Research Center for Environmental Health Jingwen Chen organic phosphorus hydrolysis ability
Shandong University, China Germany Dalian University of Technology, China Mang Yan, Liufang Yu, Liang Zhang, Yuexia Guo, Kewei Dai, and Yuru Chen
Maohong Fan Xuejun Wang Jianying Hu
University of Wyoming, USA Peking University, China, Peking University, China 2322 Microencapsulated chlorpyrifos: Degradation in soil and influence on soil microbial community structures
Chihpin Huang Lizhong Zhu Guibin Jiang
Liezhong Chen, Yanli Li, Ting Wang, Yali Jiang, Kai Li, and Yunlong Yu
National Chiao Tung University, Taiwan, China Zhejiang University, China Research Center for Eco-Environmental Sciences,
Ng Wun Jern Atomospheric environment Chinese Academy of Sciences, China 2331 Molecular characterization and mRNA expression of ribosomal protein L8 in Rana nigromaculata
Nanyang Environment & Jianmin Chen Sijin Liu during development and under exposure to hormones
Water Research Institute, Singapore Fudan University, China Research Center for Eco-Environmental Sciences,
Clark C. K. Liu Abdelwahid Mellouki Chinese Academy of Sciences, China
Qinqin Lou, Shan Cao, Wei Xu, Yinfeng Zhang, Zhanfen Qin, and Wuji Wei
University of Hawaii at Manoa, USA, Centre National de la Recherche Scientifique, Tsuyoshi Nakanishi
2340 Spatial–temporal distribution and potential ecological risk assessment of nonylphenol and octylphenol in
Hokyong Shon France Gifu Pharmaceutical University, Japan
University of Technology, Sydney, Australia, Yujing Mu Willie Peijnenburg
riverine outlets of Pearl River Delta, China
Zijian Wang Research Center for Eco-Environmental Sciences, University of Leiden, The Netherlands Ru Chen, Pinghe Yin, Ling Zhao, Qiming Yu, Aihua Hong, and Shunshan Duan
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, China Bingsheng Zhou
Chinese Academy of Sciences, China Min Shao Institute of Hydrobiology, 2348 Synthesis and characterisation of potassium polytitanate for photocatalytic degradation of crystal violet
Zhiwu Wang Peking University, China Chinese Academy of Sciences, China Mohammad Shahid, Ibrahim El Saliby, Andrew McDonagh, Leonard D. Tijing, Jong-Ho Kim,
The Ohio State University, USA James Jay Schauer Environmental catalysis and materials and Ho Kyong Shon
Yuxiang Wang University of Wisconsin-Madison, USA Hong He
Queen’s University, Canada, Yuesi Wang Research Center for Eco-Environmental Sciences, 2355 Ultrasonic preparation of nano-nickel/activated carbon composite using spent electroless nickel plating
Min Yang Institute of Atmospheric Physics, Chinese Academy of Sciences, China bath and application in degradation of 2,6-dichlorophenol
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, China Junhua Li Jingyu Su, Guanping Jin, Changyong Li, Xiaohui Zhu, Yan Dou, Yong Li, Xin Wang, Kunwei Wang,
Chinese Academy of Sciences, China Xin Yang Tsinghua University, China and Qianqian Gu
Zhifeng Yang University of Cambridge, UK Wenfeng Shangguan
Beijing Normal University, China, Environmental biology Shanghai Jiao Tong University, China 2362 Photocatalytic degradation of endocrine disruptor Bisphenol-A in the presence of prepared
Han-Qing Yu Yong Cai Yasutake Teraoka CexZn1 − xO nanocomposites under irradiation of sunlight
University of Science & Technology of China Florida International University, USA Kyushu University, Japan
Kamaraj M., Ranjith K.S., Rajeshwari Sivaraj, Rajendra Kumar R.T., and Hasna Abdul Salam
Terrestrial environment Henner Hollert Ralph T. Yang
Christopher Anderson RWTH Aachen University, Germany University of Michigan, USA
Massey University, New Zealand Jae-Seong Lee Environmental analysis and method
Zucong Cai Hanyang University, South Korea Zongwei Cai
Nanjing Normal University, China Christopher Rensing Hong Kong Baptist University,
Xinbin Feng University of Copenhagen, Denmark Hong Kong, China
Institute of Geochemistry, Bojan Sedmak Jiping Chen
Chinese Academy of Sciences, China National Institute of Biology, Ljubljana, Slovenia Dalian Institute of Chemical Physics,
Hongqing Hu Lirong Song Chinese Academy of Sciences, China
Huazhong Agricultural University, China Institute of Hydrobiology, Minghui Zheng
Kin-Che Lam Chinese Academy of Sciences, China Research Center for Eco-Environmental Sciences,
The Chinese University of Hong Kong Chunxia Wang Chinese Academy of Sciences, China
Hong Kong, China National Natural Science Foundation of China Municipal solid waste and green chemistry
Erwin Klumpp Gehong Wei Pinjing He
Research Centre Juelich, Agrosphere Institute, Northwest A & F University, China Tongji University, China
Germany Daqiang Yin Environmental ecology
Peijun Li Tongji University, China Rusong Wang
Institute of Applied Ecology, Zhongtang Yu Research Center for Eco-Environmental Sciences,
Chinese Academy of Sciences, China The Ohio State University, USA Chinese Academy of Sciences, China

Editorial office staff

Managing editor Qingcai Feng
Editors Zixuan Wang Suqin Liu Zhengang Mao
English editor Catherine Rice (USA)

Aims and scope

The Journal of Environmental Sciences, an international monthly journal established since 1989 and sponsored by the Research
Center for Eco-Environmental sciences, the Chinese Academy of Sciences. Journal of Environmental Sciences is devoted to
publish original, peer-reviewed research papers and reviews on main aspects of environmental sciences, such as environmental
chemistry, soil chemistry, atmosphere chemistry, environmental biology, ecotoxicology, geosciences. The aim of the journal is to
provide a platform for the latest research advancement.
Copyright © 2014, The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Published by Elsevier
B.V. All rights reserved.
 J O U R NA L OF EN V I RO N M EN T A L S CI EN C ES 2 6 ( 20 1 4 ) 2 1 78–2 1 8 9

Available online at www.sciencedirect.com

ScienceDirect
www.journals.elsevier.com/journal-of-environmental-sciences

Invited article

Utilization of plant-based natural coagulants as future

alternatives towards sustainable water clarification

Sook Yan Choy1 , Krishna Murthy Nagendra Prasad1 , Ta Yeong Wu1 ,

Mavinakere Eshwaraiah Raghunandan2 , Ramakrishnan Nagasundara Ramanan1,3,⁎
1. Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, 47500 Bandar Sunway, Selangor, Malaysia.
E-mail: ramanan@monash.edu
2. Civil Engineering Discipline, School of Engineering, Monash University Malaysia, 47500 Bandar Sunway, Selangor, Malaysia
3. Advanced Engineering Platform, School of Engineering, Monash University Malaysia, 47500 Bandar Sunway, Selangor, Malaysia

AR TIC LE I N FO ABS TR ACT

Available online 11 October 2014 Rapid industrial developments coupled with surging population growth have complicated
issues dealing with water scarcity as the quest for clean and sanitized water intensifies
Keywords: globally. Existing fresh water supplies could be contaminated with organic, inorganic and
Chemical coagulant biological matters that have potential harm to the society. Turbidity in general is a measure
Commercialization of water cloudiness induced by such colloidal and suspended matters and is also one of the
Constraints major criteria in raw water monitoring to meet the stipulated water quality guidelines.
Fruit waste Turbidity reduction is often accomplished using chemical coagulants such as alum. The use
Protein of alum is widely associated with potential development of health issues and generation of
Turbidity reduction voluminous sludge. Natural coagulants that are available in abundance can certainly be
considered in addressing the drawbacks associated with the use of chemical coagulants.
Twenty one types of plant-based natural coagulants categorized as fruit waste and others
are identified and presented collectively with their research summary in this review. The
barriers and prospects of commercialization of natural coagulants in near future are also
discussed.
© 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.
Published by Elsevier B.V.

Contents

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2179
1. History of coagulants used in water clarification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2179
2. Downsides of chemical coagulants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2179
3. Advantages of plant-based natural coagulants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2180
4. Classification of plant-based coagulants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2180
4.1. Fruit waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2180
4.2. Others . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2181

⁎ Corresponding author. E-mail: ramanan@monash.edu (Ramakrishnan Nagasundara Ramanan).

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http://dx.doi.org/10.1016/j.jes.2014.09.024
1001-0742/© 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
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 JO U R N A L OF EN V I RO N M EN T A L S CI E NC ES 2 6 ( 20 1 4 ) 2 1 7 8–2 1 8 9 2179

5. Constraints in commercialization of natural coagulants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2184

6. Prospects of natural coagulants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2184
7. Limitations and further improvements on the current natural coagulant studies . . . . . . . . . . . . . . . . . . . 2185
8. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2187
Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2187
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2187

Introduction resulted in the introduction of polymerized aluminium coagu-

Water, air and food are among some of the essential elements in life.
Yet, these important commodities have been taken for granted as
continuous industrial development becomes the frontier of today's
world. As a result, environmental pollutions and the shrinkage of
precious commodities have affected the lives of many. The world
consists of a major portion (about 71%) of water but freshwater
contributes to only a minor fraction of 2.5%. However, more than
60 billion m3 a year of freshwater is needed to cope with the annual
global population growth of 80 million people. In Asia alone, urban
populations can be expected to double in the year 2000–2030
(Martine, 2007) and water scarcity is now a global pandemonium
not limited to arid zones (Wu et al., 2013). Continuous population
growth, increasing standard of living, climate change, industrializa-
tion, agriculture and urbanization are triggering the decrease in
water resource worldwide (Wu et al., 2013). The remaining propor-
tion of water sources could be contaminated to certain extents
rendering them to be unsafe for direct consumptions. The contam-
ination of raw water could be due to human intervention,
geopogenic source (water-rock interaction) or climate conditions in
one country (Nasrabadi and Abbasi Maedeh, 2014).
As such, adequate water treatment and sanitation are essen-
tial to remove turbidity, impurities and other pathogenic bacteria
which can be guided through the addition of coagulants. Due
to its simplicity and effectiveness, coagulation and flocculation
are one of the most widely adopted techniques to restore the
palatability and improve the esthetic appearance of turbid
water. This technique results in the destabilization of colloidal
particles and subsequently, the increment in particle size for the
ease of sedimentation. The figurative illustration of the coagu-
lation and flocculation process via the charge neutralization
mechanism has been shown elsewhere (Choy et al., 2013).
Despite that, 67% of the worldwide population could still be
deprived to the access of adequate sanitation in the year 2030
(World Water Assessment Programme, 2009).
1. History of coagulants used in water clarification
Coagulation and flocculation could be achieved using either
natural coagulants or chemical-based coagulants. Among the
two, natural coagulants have long been acknowledged for
their application in traditional water purification which was
evident from various ancient records cited (Dorea, 2006;
Bratby, 2006). On the other hand, the global dissemination of
alum as a coagulant for public water supply occurred back in
the 19th century (Jahn, 2001). Since then, China has become
the world's earliest alum user for the clarification of water
followed by other nations globally. In the United States, metal
lants. Polyaluminium chloride (PACl) has garnered a growing
market owing to its higher superiority and lower consumption
of alkalinity. Organic synthetic polymer flocculants such as the
more common polyacrylamide are also available in the market;
offering a wider selection of chemical coagulants to cater for the
diverse requirements of the individual water treatment plants
(Bolto and Gregory, 2007).
With the invasion of chemical coagulants, traditional
water clarification methods using natural coagulants are no
longer practiced except in rural and developing countries
which have limited accessibility to these chemicals. This
marked the beginning of a paradigm shift towards the
dependence of chemical coagulants in treating turbid water.
Over the years, this scenario has gradually led to stagnation in
the development of natural coagulants. An infinite gap has
since existed drawing obvious lines between these two
coagulant variants as the traditional water clarification
method becomes obsolete.
2. Downsides of chemical coagulants
Despite the superiority of chemical coagulants in treating turbid
water, they are still lacking in terms of green chemistry. In the
1960s, detrimental effects of chemical coagulants on the human
health were published (Simate et al., 2012). Residual aluminium
in alum treated water has been the center of debate as it is
linked to serious health issues such as the development of
Alzheimer's disease (AD) (McLachlan, 1995; Flaten, 2001;
Gauthier et al., 2000; Walton, 2013; Rondeau et al., 2000) and
senile dementia (Rondeau et al., 2001). The results from a
number of epidemiological studies and clinical observations
have suggested at least 70% of positive correlations between the
presence of aluminium in drinking water and AD (Doll, 1993;
Flaten, 2001).
However, contradictory results have also been reported due
to the absence of substantial evidence in several instances
(Martyn et al., 1997; Forster et al., 1995; Wettstein et al., 1991).
Thus, a conclusive link between aluminium and the progres-
sion of AD could not be determined at this point of time.
Nevertheless, these positive results obtained should not be
dismissed where attention must be given to control the amount
of residual aluminium in treated water (WHO, 2011). To
minimize this risk factor over prolonged duration of consump-
tion, threshold aluminium concentration values in treated
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coagulants such as ferric salts have their reign in water water have been reported to be 0.02 mg/L (Doll, 1993) and
treatment since 1880. As the performance of these customary 0.1 mg/L (McLachlan, 1995; Rondeau et al., 2000). While the
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chemical coagulants is dubious in certain working conditions suggested aluminium concentration by WHO is 0.2 mg/L,
such as low temperatures, on-going developments have certain countries have adhered to much stricter guidelines
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2180 J O U R NA L OF EN V I RO N M EN T A L S CI EN C ES 2 6 ( 20 1 4 ) 2 1 78–2 1 8 9
(Simate et al., 2012). Even so, a mean aluminium concentration
ranging from 0.01 to 0.15 mg/L is common (Doll, 1993), slightly
above the threshold values which could increase the probability
of AD incidence with respect to drinking water.
Other concerns over the presence of residual aluminium in
drinking water such as reduced water disinfection efficiency
have been well documented (Driscoll and Letterman, 1995).
Synthetic organic polymer for instance acrylamide has strong
neurotoxic and carcinogenic effects. Unreacted chemical mono-
mers and the by-products of polymers in water could also
threaten the human health (Bratby, 2006). As for iron salts,
careful process controls are necessary as excessive iron residual
in the treated water will lead to highly visible rust or blood
colored stains (Gebbie, 2005) caused by the hydrolysis of iron
salts.
Another major drawback of hydrolyzing metal coagulants is
the generation of voluminous hydrous oxide sludge which is
non-biodegradable due to the nature of the coagulant. For
instance, 99% of the alum sludge is made up of water (Gregory,
2006) and alum sludge is rather hard to dewater. Alum sludge
also has the disadvantage of causing phosphorus uptake from
plants due to inorganic phosphorus absorption which leads to
aluminium phytotoxicity (Cornwell, 1999). It has been estimat-
ed that a typical water treatment plant with the treatment
capacity of 190 million L per day using alum would result in at
least 3 tonnes of dry solid waste; accumulating to more than
1000 tonnes annually (Gruninger, 1975). This finding indirectly
translates to the excessive sludge input to existing landfills
which are currently being reported to be insufficient to cater for
the rapidly growing amount of sludge produced. Moreover,
treatment of highly turbid water requires several proteolytic
additives along with alum making it an expensive process. Such
complicated methods for treatment of highly turbid water are
difficult to adopt in poor or developing countries, where
cost-effective and simple drinking water treatment methods
are needed (Bodlund et al., 2014). Owing to the downfalls of
chemical coagulants, there is a need to consider other potential
alternatives for water clarification to minimize the environ-
mental damages and to safeguard the wellbeing of human
population.
3. Advantages of plant-based natural coagulants
In contrast to chemical coagulants, plant-based natural coagu-
lants are safe (Asrafuzzaman et al., 2011), eco-friendly and
generally toxic free (Bratby, 2006; Swati and Govindan, 2005).
Natural coagulants have been found to generate not only a
much smaller sludge volume of up to five times lower
(Ndabigengesere et al., 1995) but also with a higher nutritional
sludge value. As such, sludge treatment and handling costs are
lowered making it a more sustainable option. The raw plant
extracts are often available locally and hence, a low cost
alternative to chemical coagulants. Since natural coagulants
do not consume alkalinity unlike alum, pH adjustments can be
omitted and this provides extra cost savings. Natural coagu-
lants are also non-corrosive (Swati and Govindan, 2005) which
eliminate the concerns of pipe erosions. Fig. 1 summarizes the
benefits of using natural coagulants as an alternative to
chemical coagulants in water clarification process.
Till date, reviews on plant-based natural coagulants were
mainly focused on the four most common natural coagulants
which included Moringa oleifera seeds, Nirmali seeds, tannin and
Opuntia ficus indica cactus in the water and wastewater
treatments (Yin, 2010; Vijayaraghavan et al., 2011). In addition
to the current database of studied natural coagulants, an
extensive overview on the indigenous plants used by rural
Africans in water purification has been provided (Yongabi,
2010). Recently, an extensive review covering the studied
vegetables and legumes has provided an insight to the less
commonly known water clarifying agents (Choy et al., 2013).
Apart from the mentioned plant-based coagulants, little is
known about other plant categories with notable water
clarification properties. Hence, there is a need to account for
such coagulants and to identify the key findings obtained from
various coagulation studies conducted previously. This review
work can serve as a compendium of 21 types of plant-based
coagulants grouped under two main categories namely fruit
waste and others (cactus, cereals, fungus, nuts, shrubs and
spice). Further, the challenges which kept the commercializa-
tion of natural coagulants at bay are also highlighted.
4. Classification of plant-based coagulants
A majority of the plant-based coagulants incorporated in this
current review is made up of fruit waste which ultimately
forms a viable source of raw materials for further applications.
The remaining coagulants fall under various categories of
cactus, cereals, fungus, nuts, shrubs and spice. However, they
will be jointly reviewed as others to provide a general
overview on the coagulation studies conducted. The back-
ground information and physicochemical properties for the
respective plant-based coagulants could be beneficial to
provide an insight to its nature and the probable active
coagulation agents present (refer to supporting information).
4.1. Fruit waste
Fruits are rich sources of vitamins and nutrients which can be
consumed fresh, dried, or processed with diverse demands in
the food industries. A significant amount of up to 50% of the
total fruit weight is made up of fruit waste such as peels and
seeds that are commonly non-edible. These large proportions
of waste are generally discarded into the environment as they
lack in commercial values. In Malaysia, the habit of com-
posting and waste recycling is not yet a widely adopted
method and ultimately resorting to the easiest disposal
method; landfilling (APO, 2007). Close to 1000 million tonnes
of agricultural waste are being generated internationally in a
yearly basis and of this, about 1.2 million tonnes are being
discarded into landfills in Malaysia alone (Agamuthu, 2009).
The mishandling of fruit waste could be detrimental to the
environment due to potential leaching into soil and water
sources leading to further pollution (Hacker et al., 2009).
Although fruit waste is generally discarded, it is worth-
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while to note that some waste such as seeds and barks have
diverse folkloric medicine applications. Findings till date have
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suggested the use of 9 fruit waste in treating turbid synthetic
water, raw surface water and waste water. These are the
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 JO U R N A L OF EN V I RO N M EN T A L S CI E NC ES 2 6 ( 20 1 4 ) 2 1 7 8–2 1 8 9
Fig. 1 – Advantages of natural coagulants (NCs) over chemical coagulants.
seeds of Carica papaya, Feronia limonia, Mangifera indica, Persea
americana, seeds and pollen sheath of Phoenix dactylifera,
Prunus armeniaca, Tamarindus indica, the peels of Citrus sinensis
and the foliage of Hylocereus undatus (Table 1). Among these,
P. armeniaca has been used as primary coagulants during the
ancient times in China and Egypt with histories dating back to
the 1100s (Jahn, 2001). Generally, turbidity reduction of greater
than 80% for most of the fruit waste coagulants can be
achieved although several exceptions exists which is likely
unfavorable for the treatment of drinking water (Table 1). The
various forms of natural coagulants used could also have
influenced the turbidity removals reported. For instance, the
blending of T. indica seeds in water (Thakre and Bhole, 1985)
could be more efficient compared to aqueous extraction using
the powdered seeds (Swati and Govindan, 2005); underlying
the importance of the pre-treatment step.
The colloids present in foliage of H. undatus are cationic
which is similar to the seeds of M. oleifera. Thus, adsorption
and charge neutralization have been proposed as the possible
coagulation mechanism leading to formation of flocs (Som et
al., 2007; Idris et al., 2013). Proteins and polysaccharides are
among some of the natural polymers present in fruit waste.
The size of these polymers can be expressed in terms of
molecular weight where a larger value would correspond to a
longer polymeric chain. This would be advantageous for
coagulation as the number of active sites available for particle
adsorption will be increased. Although the polysaccharide of
T. indica has a higher molecular weight of around 720–880 kDa,
the obtained turbidity removals were no greater than that of
M. oleifera with dimeric proteins of 13 kDa (Mirhosseini and
Amid, 2012; Ndabigengesere et al., 1995). The proximate
compositions of protein and carbohydrate between these
seed types could be the underlying factor leading to the
variations in treatment efficiencies as observed.
Apart from turbidity, commonly present bacteria notably
Escherichia coli and Staphylococcus aureus in surface water must
also be removed to meet the stipulated drinking water
standards. Some fruit waste has notable coagulation activities
2181
while others could be more superior in terms of bacteriostatic
activities. An example of such coagulants is the extracts of
P. americana which is rich in antimicrobial agents such as
saponins, phenols and flavonoids (Arukwe et al., 2012). Most
of the fruit waste coagulants were also found to exhibit potent
bacteriostatic properties which retarded the proliferation in
bacteria (Osato et al., 1993; Dubey et al., 2011; Ashok kumar et
al., 2011; Engels et al., 2009;Perveen et al., 2012; Al-daihan and
Bhat, 2012; Bhat and Al-Daihan, 2012; Yiğit et al., 2009).
Nevertheless, secondary disinfection would be crucial to
attain a fecal coliform level of zero colony forming units
(cfu)/100 mL in the treated water to meet the quality of the
drinking water standards (Yongabi et al., 2011b; Pritchard et
al., 2009). Based on the worthwhile usage of fruit waste as
potential natural coagulants along with the potency against
selected bacteria, the overall economics as well as sustain-
ability of the fruit farming and juicing industries can be
enhanced.
4.2. Others
An assortment of plants other than fruit waste has also been
discovered over the recent years for treating both raw surface
and synthetic turbid water. This 12 other plants can be further
categorized into the various classifications of cactus, cereals,
fungus, nuts, shrubs and spice (Table 2). A majority of these
plants are familiar as commercial food sources while some
are distinguished for their traditional medicine applications
such as Strychnos potatorum (Ruijter, 2008) and Cassia alata
(Aweng et al., 2012).
Table 2 shows the summary of research highlight for
each of the plant-based coagulants classified as others: cacti
mucilage and extract from the pads of Cereus repandus,
Stenocereus griseus and O. ficus indica, the seeds of Oryza sativa
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and Zea mays which form the backbone of agricultural
industries, the sclerotium of Pleurotus tuber-regium or King
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Tuber Mushroom which is the only fungus tested till date, the
hard nuts of Jatropha curcas and S. potatorum, several species of
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Table 1 – Research summaries of fruit waste used as plant-based natural coagulants.

No. Coagulant Type of sample Initial conditions Optimized parameters

Name Form used Turbidity pH Others Dosage pH Turbidity

(NTU) (mg/L) reduction

1 Carica papaya A, F, J RSW (storm water) 119 100 seeds/100 L 90%

seeds
F, H1, K, E SW (metal 5 0.5 g/100 cm3
aqueous solution)

2 Citrus sinensis C1/C2, F RSW 32 9.4 (H4) Alkalinity: 195 mg/L 4000 ~85%
peel/skin
3 Feronia E, F, H2, I, L1 SW (clay) 100 20 8.0 ~77%
limonia seeds
E, F, H2, I, L1 SW (clay) 100 9 + 20 alum ~97.5%
(as CA which
is added first)
4 Hylocereus E, F LCE 7243 7.3 COD: 7857 mg/L 300 10 99.5%
undatus foliage BOD:
1327 mg/L
SS: 1208 mg/L
B, D, E, F LCE 7243 7.3 COD: 7857 mg/L 500 10 99.7%
SS: 1208 mg/L

5 Mangifera E, F, G RSW (Nile River) 6.5 TC: 5000 MPN/100 mL 30 54% (at clarri-
indica seeds flocculator)
Mangifera A, E, F, G SW (Kaolin) 90 0.5 mL/L 13 98.65%
indica kernels
6 Persea F, J RSW (storm water) 119 100 seeds/100 L 64.2%
americana seeds

7 Phoenix dactylifera C3, E, F, L2, G1 SW (Bentonite) 75 ± 10 30 94% (at

date seeds 120th min)
Pollen sheath C3, E, F, L2, G SW (bentonite) 300 ± 10 90 93% (at
120th min)

8 Prunus E, F, G RSW (Nile River) 6.5 TC: 7000 MPN/100 mL 30 54% (at clarri-
armeniaca seeds FC: 800 MPN/100 mL flocculator)
9 Tamarindus E, F, H2, I, L1 SW (clay) 100 mg/L 20 7.5 80%
indica seeds
E, F, H2, I, L1 SW (clay) 100 mg/L 3 + 20 alum 98% (as CA
which is
added first)
E, F, G, L1 SW (Kaolin) 76–79 5 8 69%

E, F, G, L1 RSW (Pond) 94.7 8 2 96% (as CA)

E, F TWW TSS: 425 mg/L 14,000

DO: 1.8 mg/L
A: deshelled, B: removal of thorns, C: washed with: (1. formaldehyde, 2. acid-alkaline, 3. water), D: cut, E: dried, F: pulverized, G: extracted with
water, H: soaked in: (1. HNO3, 2. water), I: blended in water, J: sprinkled onto samples, K: filtered and rinsed with water, L: added with: (1. HCl, 2.
NaOH).
RSW: raw surface water, SW: synthetic water, CA: coagulant aid, LCE: latex concentrate effluent, COD: chemical oxygen demand, BOD: biological
oxygen demand, SS: suspended solids, MPN: most probable number, TC: total coliform, FC: fecal coliform, TWW: tofu waste water, TSS: total
suspended solid, DO: dissolved oxygen.

shrubs including leaves from the ornamental shrub of Cassia (Al-Samawi and Shokralla, 1996). This marked the first
alata, juice from the roots of Maerua subcordata and seeds of reported natural plant-based coagulant used for water treat-
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Prosopis juliflora as well as the seeds from the conventional ment worldwide (Yin, 2010).
spice of Trigonella foenum-graecum. Among them, S. potatorum Generally, turbidity reduction of at least 80% has been
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has been used as water clarifiers since over 4000 years reported when these plant-based coagulants are used under
according to the historical records of Sanskrit writings optimized working conditions although few exceptional
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Findings/claims References

• E. coli counts reduced by 75.3% Yongabi et al. (2011b)

1 • Total heterotrophic bacterial counts reduced by 71.7%
• The maximum metal adsorptions at the optimized contact time of 90 min: Egila et al. (2011)
→Lead, Pb: 4.1 mg/100 cm3
→ Manganese, Mn: 3.6 mg/100 cm3
• Smaller ionic size of Mn resulting in faster adsorptions
2 • Highly efficient in the removal of alkalinity Sowmeyan et al. (2011)
• Not effective for the removal of iron and chlorides
3 • Maximum turbidity removal of ~88% at a dosage of 60 mg/L for initial turbidity of 1600 NTU Bhole (1995)
• Maximum turbidity removal of ~99.8% at a dosage of 30 mg/L + 60 mg/L alum for initial turbidity of 1600 NTU
• Efficiency at different sequence of coagulant addition:
→ ~95% with the addition of alum followed by coagulant aid
→ 93% with the simultaneous addition of both coagulants
4 • Removal of COD by 97.7% Som et al. (2007)
• Removal of BOD by 86.9%
• Removal of SS by 94.5%
• Efficiency removals below 90% and 80% for turbidity and SS respectively at lower pH (2–9)
• Removal of COD by 94.7% Idris et al. (2013)
• Removal of SS by 88.9%
• 2 ‘peak’ values existed at pH 3 and pH 10 indicating the higher removal efficiencies
5 • At the plant outlet, total coliform counts reduced by 99.6% Ali et al. (2008)
• Treated water has a greenish color despite the high removal of various algae groups
• Coagulation activity is comparable to alum at 99% Qureshi et al. (2011)
• Removal efficiency generally increased with pH increment of the water; lowest at pH 7
6 • E. coli counts reduced by 84.9% Yongabi et al. (2011b)
• Total heterotrophic bacterial counts reduced by 75.5%
• Coagulants exhibited antimicrobiological properties (reduction in microbes count) but insufficient to meet the
WHO standards
7 • 90% turbidity reduction at 60 mg/L for initial turbidity of 150 ± 10 NTU Al-Sameraiy (2012)
• 83% turbidity reduction at 80 mg/L for initial turbidity of 300 ± 10 NTU
• Performed better than pollen sheath at an initial turbidity of 75 ± 10 NTU
• 91% turbidity reduction at 20 mg/L for initial turbidity of 75 ± 10 NTU
• 92% turbidity reduction at 50 mg/L for initial turbidity of 300 ± 10 NTU
• More superior than date seeds at initial turbidities of 150–300 ± 10 NTU
8 • At the plant outlet, total coliform counts reduced by 99.7% Ali et al. (2008)
• Fecal coliform counts reduced by 99.5%
9 • Maximum turbidity removal of 92.3% at a dosage of 60 mg/L for initial turbidity of 1000 mg/L Thakre and Bhole (1985)
• Maximum turbidity removal of 99.8% at a dosage of 10 mg/L + 60 mg/L alum for initial turbidity of 1600 mg/L
• Efficiency at different sequence of coagulant addition:
→ 97% with the addition of alum followed by coagulant aid
→ 96% with the simultaneous addition of both coagulants
• Aging seed extracts are detected from the generation of foul smell or the formation of a floating slime layer Swati and Govindan (2005)
• Optimum shelf life of 10 days
• Bacterial reduction by 99.4%
• 1:10 is the ratio of alum to coagulant aid used
• 85% turbidity removal at 5 mg/L as primary coagulant
• Removal of TSS by 67.3% Nurika et al. (2007)
• The DO value increased by 53.9%

cases existed: O. ficus indica (Yang et al., 2007) and Z. mays (Fuentes et al., 2011; Karawya et al., 1980). The mucilage which
(Gunaratna et al., 2007; Subramanium et al., 2011). The presence consists of galacturonic acids is generally the predominant
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of active agents such as carbohydrates and proteins has been active coagulation agent present in cacti mucilage regardless of
linked to the observed purification effects in the various plants. species. The enhanced turbidity removal for Opuntia cactus
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For instance, uronic acids; a class of sugar acids detected at could be attributed to the much higher total compositions of
around 11% in both the mucilage of S. griseus and O. ficus indica arabinose, galactose and rhamnose which have been found to
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have synergistic effects with galacturonic acids in promoting
coagulation (Miller et al., 2008). The coagulation mechanism
was then deduced to be adsorption and bridging based on the
characteristics of the mucilage which is an anionic polyelectro-
lyte. Also negatively charged in nature, the main functional
groups of COO− and OH− in the polyelectrolyte of the seeds of S.
potatorum have been found to be responsible for the reported
coagulation activities (Subbaramiah and Rao, 1937; Tripathi
et al., 1976).
In contrast, the coagulation mechanisms in the seeds of J.
curcas were believed to be adsorption and charge neutraliza-
tion due to close similarities of the cationic active coagulation
agent to that in M. oleifera (Abidin et al., 2011). Unlike most of
the plant-based coagulants listed (Table 2), cereal crops are
largely made up of carbohydrates with up to 94% of the total
dry weight (Oko and Ugwu, 2011; Sobukola and Abayomi,
2011). Starch which is a form of carbohydrate has been
identified as the possible active agent leading to coagulation
in such coagulants (Raghuwanshi et al., 2002). Likewise, the
excellence of M. subcordata in removing turbidity could also be
due to the presence of polysaccharides mainly amylopectin
(Mavura et al., 2008). As they served as bridges to link colloidal
particles together, the increment in floc size would improve
the overall precipitation process (Göttsch, 1992). Apart from
starch, phytochemicals such as tannins in plants have also
been recognized as active agents in promoting coagulation;
suggesting the same for P. tuber-regium which contained this
active agent (Ikewuchi and Ikewuchi, 2008; Akindahunsi and
Oyetayo, 2006).
Besides notable turbidity removal properties, selected plant-
based coagulants are also capable of removing heavy metals
(Table 2). Studies have also demonstrated that Z. mays were able
to remove at least 15% of lead, chromium and hardness from
river water with larger fractions of cadmium and zinc removals
(Subramanium et al., 2011). However, the results obtained were
less superior in comparison to M. oleifera. The formaldehyde
washed seeds of S. potatorum displayed superiority with
alkalinity removals up to 70% while the acid-alkaline washed
have successfully removed more than 25% of magnesium and
hardness (Sowmeyan et al., 2011). Much smaller removal
proportions for other contaminants such as fluoride and iron
have also been obtained. Additional treatment benefits such
as the removal of bacteria (Shilpa et al., 2012; Swati and
Govindan, 2005): E. coli (Tripathi et al., 1976) and fecal coliform
(Yongabi, 2006; Yongabi et al., 2011a; Pritchard et al., 2009)
have been demonstrated. The presence of phytochemical
constituents such as alkaloids and tannins (Ejelonu et al.,
2010; Mallikharjuna et al., 2007) could most likely have
contributed to the observed antimicrobial activities in plant-
based coagulants.
5. Constraints in commercialization of
natural coagulants
Although various indigenous plant extracts have been proven
to possess coagulative properties in removing turbidity
(Sowmeyan et al., 2011), not many has reached the phase of
commercialization. The four main constraints hindering the
commercialization of natural coagulants: financial, research
and development, market awareness as well as regulatory
approval have been well documented in the literature for the
case of M. oleifera seeds (Sutherland et al., 2001). On top of the
scientific aspects, the problems encountered with the har-
vesting and processing of the plant extracts were also
highlighted. Financial freedom is a common barrier faced as
potential investors are usually hesitant to invest in new
products. By understanding the vulnerabilities and risks
involved, the development of natural coagulants would be
improved.
The research and development area holds the key in
understanding the scientific aspects of the natural coagu-
lants of interest. It also serves as a platform and backbone for
the identification of strengths, limitations, areas which
require fine-tuning as well as process improvements. The
existing results reported are largely confined to laboratory
scale which may be lacking in terms of process scalability
for real time applications. A breakthrough in this matured
market can begin by emphasizing the blending of natural and
chemical coagulants. Natural coagulants, a kind of polymers
are more than often used in conjunction with chemical
coagulants as they exhibited restricted turbidity removal
efficiencies when used alone. Likewise, the feasibility of
using plant derived coagulants may also be questionable due
to their suitability under various working conditions.
In addition, the response and needs of the market will
affect the outlook and demands of natural coagulants as a
probable replacement of chemical coagulants. This is so as
the misconception on the feasibility of natural coagulants by
both consumers and suppliers would lead to an inaccurate
prediction of the realistic target market. An evaluation on the
economic, social and environmental aspects which could be
used as a sustainability indicator would be useful with the aid
of a pilot plant study. Approval from the local governing
bodies must also be granted for successful commercialization
of any new products. However, it may not be easy to obtain an
approval as stringent screenings and documentations could
be needed to ensure product compliance to the respective
standards (Sutherland et al., 2001). Tax rebates and subsidy
schemes could be offered as a form of driving incentive to
motivate water treatment operators to adopt the use of
natural coagulants.
While the commercialization of natural coagulants will not
be an overnight success, these small steps taken would
progressively bridge the gaps and limitations encountered.
Fig. 2 summarizes these factors which must be taken into
considerations for successful commercialization of natural
coagulants.
6. Prospects of natural coagulants
Despite the constraints faced in commercialization, tannin
extracts have been used extensively in the potable and
wastewater treatment industries (Bratby, 2006). The tannin
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extract from the barks of Acacia mearnsii tree have been
successfully commercialized as Floccotan (Bratby, 2006).
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Another tannin commercialization is evident under the
ECOTAN series (Servyeco Group, 2008). Tannins which are a
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Table 2 – Highlights of plant-based natural coagulants classified as others.

No. Plant name Highlights Reference

Cactus
1 Cereus repandus Similar residual turbidity with alum (< 10 Nephelometric Turbidity Units, NTU) but Diaz et al. (1999)
at a much
lower coagulant dosage of 20 mg/L
2 Stenocereus griseus Exhibited color reduction from 50 to 25 units in treated water Fuentes et al. (2011)
3 Opuntia ficus indica Turbidity removal lowest at pH 6 Zhang et al. (2006)
Coagulation properties might be contributed by the presence of Miller et al. (2008)
mucilage, especially by the galacturonic acid component Mane et al. (2011)
Demonstrated nickel and chromium removal by at least 65% Shilpa et al. (2012)
Reduction of bacteria up to 20%
Cereals
4 Oryza sativa Maximum turbidity removal of 96.4% is achievable for initial Thakre and Bhole (1985)
turbidity of 1000 mg/L
5 Zea mays Exhibited more than 20% of cadmium and zinc removal Subramanium et al. (2011)
Fungus
6 Pleurotus tuber-regium Extremely low sensitivity to Escherichia coli; suggesting the absence of Yongabi et al. (2011a),
notable antibacterial activity Yongabi (2006)
Nuts
7 Jatropha curcas Removal of Escherichia coli and fecal coliforms by at least 60% Yongabi et al. (2011a),
Similar turbidity removal (~ 99%) in both the conventional and Yongabi
the assisted blending methods (2006), Pritchard et al. (2009)
Abidin et al. (2013)
8 Strychnos potatorum Reduction of E. coli and bacteria by at least 40% Tripathi et al. (1976), Swati
Removal of hardness and magnesium up to 30% and
Govindan (2005)
Sowmeyan et al. (2011)
Shrubs
9 Cassia alata No potential for iron and manganese removal but commendable Aweng et al. (2012)
for suspended solids
10 Maerua subcordata Contains relatively large amounts of proteins and polysaccharides Mavura et al. (2008)
(mostly amylopectin which is responsible for the coagulation properties)
11 Prosopis juliflora Works best at pH 11 Diaz et al. (1999)
Spice
12 Trigonella Lowest suspended solids removal at pH 4 Mishra et al. (2003)
foenum-graecum Active coagulant is temperature and pH stable Ramamurthy et al. (2012)

form of natural high molecular weight polysaccharide is made
as a natural water clarifier and has been reported to work in
all types of waste waters. Hence, it is not surprising that
tannins became the first commercialized natural coagulant
instead of other plant sources.
Compared to alum, enhanced turbidity and color removal
from water sources have been reported owing to the use of a
weakly basic polymer which is formed by reacting tannin with
formaldehyde and aminoethanol (Bolto and Gregory, 2007).
Besides that, the use of tannin compounds is advantageous
over the studied natural coagulants as it requires no further
purification and isolation processes which could be both time
and economically challenged. Its relatively simple extraction
methods made it highly favored over other mucilaginous
plant extracts such as the cactus species which is beneficial to
the research and development aspect. Tannins which are
polyphenol compounds extracted from plants are also com-
monly ingested through dietary intakes and are ‘Generally
Recognized as Safe’ (GRAS) for the use as food additives in
various products (United States Environmental Protection
Agency, 2006). Despite that, contradictory effects on the
The features highlighted could be viewed favorably as a
selling point to develop and promote tannins as a commercialized
plant-based natural coagulant in line with the urgency towards
sustainable water treatment. As potential investors, stake-
holders and suppliers discover the social-economic and envi-
ronmental values in developing tannins, the financial aspect
and market awareness are resolved easily; contributing to
successful commercialization.
7. Limitations and further improvements on the
current natural coagulant studies
Natural coagulants are known for their efficiency in reducing
turbidity but little is known about the characteristics and
properties of the resultant flocs formed. The knowledge on
these parameters such as floc strength, structure and com-
pactness could be the key in establishing the probable
coagulation theory (Li et al., 2006). The scanning of flocs
generated upon coagulation could be performed via various
microscopy imaging techniques such as Transmission Elec-
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human health have been reported when tannins are consumed tron Microscopy. This could provide an overview on the
in large quantities (Chung et al., 1998). However, the acceptable structure and surface characteristics which may lead to better
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daily intake (ADI) for tannins has yet to be established according understanding on the bonding of colloidal particles (Idris et
to the toxicological evaluation report (FAO, 1970). al., 2013).
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Fig. 2 – Factors hindering the commercialization of natural coagulants.
Extensive studies to conclude the respective coagulation
mechanism involved in each of the plant-based coagulants
are also limited. The aggregation of colloidal particles leading
to water clarification could be a result of one or more
coagulation mechanisms. Determination of the exact chem-
ical constituent responsible in inducing particle agglomera-
tions is extremely difficult owing to complexity of the process
and probable synergistic effects among the components
present. Further work involving isolation, purification and
characterization of the active agents would be beneficial to
identify the underlying compounds leading to coagulation. Only
a handful of such studies have been performed over the years
which include the studies on seeds of M. oleifera (Ghebremichael
et al., 2005; Okuda et al., 2001), Moringa concanensis (Sathiyabama,
2012), Vigna unguiculata and Parkinsonia aculeata (Marobhe et al.,
2007) and more recently, endosperm of Cocos nucifera (Fatombi
et al., 2013). Direct extraction of these compounds could
enhance the effectiveness of the overall coagulation process
owing to increased relative concentration of the agents. In fact,
the coagulant dosage required to achieve optimum coagulation
was also significantly reduced by 5 to 10 times when purified
protein of V. unguiculata was used instead of the crude extract
(Marobhe et al., 2007).
Although natural coagulants are green in nature, the use of
crude extracts has been related to the leaching of organic
matters into treated water; quantifiable in terms of total
organic carbon (TOC), dissolved organic carbon (DOC) and
others. Crude extracts contained various inorganic and organ-
ic biomolecules such as lipids which do not participate in
active coagulation (Ghebremichael, 2007). Increased levels of
organic matters in treated water would have serious implica-
tions on the subsequent disinfection process using chlorine.
These organic matters can act as precursors to the formation
of trihalomethanes (THMs) in which many are carcinogenic
(Duan and Gregory, 2003) and might also increase the required
chlorine demand for adequate disinfection (Anastasakis et al.,
2009). The use of purified active agents could tackle this
undesirable and problematic issue (Ghebremichael, 2007;
Sathiyabama, 2012). Protein purification via ion exchange
has successfully eliminated the additional nutrient and
organic loading introduced by the seeds of M. oleifera
(Ghebremichael, 2007). Partial purification of active agents in
legume; Phaseolus vulgaris has also been rewarding as the
organic loading content has been reduced by at least 16 times
in contrast to the use of crude extracts (Antov et al., 2010).
However, extraction methods that are economically feasible
would be needed for successful commercialization of natural
coagulants.
While the reviewed plant-based coagulants could be
considered as potential alternatives to chemical coagulants,
several issues such as the feasibility and sourcing of the
plants could be challenging. For instance, the toxicity of the
J. curcas seeds has raised safety issues for its application as a
natural coagulant for the treatment of drinking water
(Pritchard et al., 2009). One probable solution to counter for
the potential of poison leaching into water is to opt for the
non-toxic variety of seeds as they are safe for human
consumptions. Even so, further coagulation studies would be
necessary as this seed variety has yet to be tested for its
potential in water clarifications although both variants have
similarly high protein contents. As certain fruits are seasonal,
the availability of these plant-based coagulants throughout
the year could be dubious. The respective parts of the studied
coagulants such as foliage and pollen sheath would also be
difficult to collect for mass consumptions in real time
applications. Perhaps, plant-based coagulants that are avail-
able yearly with notable coagulation activities can be consid-
ered for industrial scale applications. The use of cereal crops
as natural coagulants could be complicated by the escalating
prices due to the growing world population and challenges in
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keeping up with the agricultural productivity. In fact, the
universal rice utilization is forecasted to rise by 1.4% in years
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2012 and 2013 although shrinkage by 2.7% in world cereal
production can be expected (FAO, 2012). In some cases, the
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sole use of natural coagulants may be inadequate to achieve
the desired turbidity removal. However, they can certainly be
added as an aid to the latter (Bhole, 1995; Thakre and Bhole,
1985; Raghuwanshi et al., 2002; Swati and Govindan, 2005).
The blending of both coagulant types can reduce the reliance
on chemical coagulants while addressing the lower turbidity
removal and unavailability of certain plant-based coagulants
which may be seasonal.
8. Conclusions
Plant-based natural coagulants have garnered growing inter-
ests from researchers over the years due to their biodegradabil-
ity and environmental friendly nature. The diverse types of
plant-based coagulants including fruit waste and others (cac-
tus, cereals, fungus, nuts, shrubs and spice) as discussed in this
work have demonstrated promising coagulation activities in
treating turbid water. In addition, a number of these coagulants
can also provide primary disinfection against some of the
commonly present bacteria in surface water while others have
been found to be capable of removing heavy metals. For
commercialization, natural coagulants must be able to pene-
trate into the well-established market highly empowered by
chemical coagulants among the local communities. Although
natural coagulants are generally less superior in contrast to
alum, the blending of both coagulants in certain proportions
may prove beneficial in maintaining the water treatment
efficiency while gradually reducing the reliance on chemical
coagulants. Unlike chemical coagulants, studies on natural
coagulants have mainly focused on the turbidity removal from
synthetic and raw surface water with ample room for further
research. However, in-light with the health and environmental
concerns of chemical coagulants, there is an immediate need
for detailed research to identify the coagulation mechanism
and developing feasible methods for pretreating natural coag-
ulants as a sustainable alternative to chemical coagulants.
Acknowledgments
The authors are grateful to Monash University Malaysia for
providing the research scholarship and essential support for
this work. Sook Yan Choy is a recipient of the Higher Degree
by Research Scholarship (HDR) awarded by Monash University
Malaysia.
REFERENCES
Abidin, Z.Z., Ismail, N., Yunus, R., Ahamad, I.S., Idris, A., 2011. A
preliminary study on Jatropha curcas as coagulant in
wastewater treatment. Environ. Technol. 32 (9), 971–977.
Abidin, Z.Z., Mohd Shamsudin, N.S., Madehi, N., Sobri, S., 2013.
Optimisation of a method to extract the active coagulant agent
from Jatropha curcas seeds for use in turbidity removal. Ind.
Crop. Prod. 41 (1), 319–323.
Agamuthu, P., 2009. Challenges, opportunities in agro-waste
management: an Asian perspective. Inaugural Meeting of First
Regional 3R Forum in Asia. Tokyo, Japan.
2187
Akindahunsi, A.A., Oyetayo, F.L., 2006. Nutrient and antinutrient
distribution of edible mushroom, Pleurotus tuber-regium (fries)
singer. LWT 39 (5), 548–553.
Al-Daihan, S., Bhat, R.S., 2012. Antibacterial activities of extracts
of leaf, fruit, seed and bark of Phoenix dactylifera. Afr. J.
Biotechnol. 11 (42), 10021–10025.
Ali, G.H., Hegazy, B.E., Fouad, H.A., Rehab, M.E., 2008. Comparative
study on natural products used for pollutants removal from
water. J. Appl. Sci. Res. 5 (8), 1020–1029.
Al-Samawi, A.A., Shokralla, E.M., 1996. An investigation into an
indigenous natural coagulant. J. Environ. Sci. Health A 31 (8),
1881–1897.
Al-Sameraiy, M., 2012. A novel water pretreatment approach for
turbidity removal using date seeds and pollen sheath. J. Water
Res. Prot. 4 (2), 79–92.
Anastasakis, K., Kalderis, D., Diamadopoulos, E., 2009.
Flocculation behavior of mallow and okra mucilage in treating
wastewater. Desalination 249 (2), 786–791.
Antov, M.G., Šćiban, M.B., Petrović, N.J., 2010. Proteins from
common bean (Phaseolus vulgaris) seed as a natural coagulant
for potential application in water turbidity removal. Bioresour.
Technol. 101 (7), 2167–2172.
APO, 2007. Solid Waste Management: Issues and Challenges in
Asia, (Tokyo).
Arukwe, U., Amadi, B.A., Duru, M.K.C., Agomuo, E.N., Adindu, E.A.,
Odika, P.C., et al., 2012. Chemical composition of Persea americana
leaf, fruit and seed. Int. J. Res. Rev. Appl. Sci. 11 (2), 346–349.
Asrafuzzaman, M., Fakhruddin, A.N.M., Alamgir Hossain, M., 2011.
Reduction of turbidity of water using locally available natural
coagulants. ISRN Microbiol. 632189. http://dx.doi.org/10.5402/
2011/632189.
Ashok kumar, K., Narayani, M., Subanthini, A., Jayakumar, M.,
2011. Antimicrobial activity and phytochemical analysis of
citrus fruit peels — utilization of fruit waste. Int. J. Eng. Sci.
Technol. 3 (6), 5414–5421.
Aweng, E.R., Anwar, I.A., Siti Rafiqah, M.I., Suhaimi, O., 2012.
Cassia alata as a potential coagulant in water treatment. Res. J.
Recent Sci. 1 (2), 28–33.
Bhat, R.S., Al-Daihan, S., 2012. Antibacterial properties of different
cultivars of Phoenix dactylifera L and their corresponding
protein content. Ann. Biol. Res. 3 (10), 4751–4757.
Bhole, A.G., 1995. Relative evaluation of a few natural coagulants.
J. Water Supply Res Technol. 44 (6), 284–290.
Bodlund, I., Pavankumar, A.R., Chelliah, R., Kasi, S., Sankaran, K.,
Rajarao, G.K., 2014. Coagulant proteins identified in Mustard: a
potential water treatment agent. Int. J. Environ. Sci. Technol.
11 (4), 873–880. http://dx.doi.org/10.1007/s13762-013-0282-4.
Bolto, B., Gregory, J., 2007. Organic polyelectrolytes in water
treatment. Water Res. 41 (11), 2301–2324.
Bratby, J., 2006. Coagulation and Flocculation in Water and
Wastewater Treatment, 2nd ed. IWA Publishing, UK.
Choy, S.Y., Prasad, K.M.N., Wu, T.Y., Ramanan, R.N., 2013. A
review on common vegetables and legumes as promising
plant-based natural coagulants in water clarification. Int. J.
Environ. Sci. Technol. 1–24. http://dx.doi.org/10.1007/s13762-
013-0446-2.
Chung, K.T., Wong, T.Y., Wei, C.I., Huang, Y.W., Lin, Y., 1998.
Tannins and human health: a review. Crit. Rev. Food Sci. Nutr.
38 (6), 421–464.
Cornwell, D.A., 1999. Water treatment plant residual
management. In: Letterman, R.D. (Ed.), Water Quality and
Treatment: A Handbook of Community Water Supplies.
McGraw-Hill, USA, pp. 16. 11–16. 51.
Diaz, A., Rincon, N., Escorihuela, A., Fernandez, N., Chacin, E.,
.cn
Forster, C., 1999. A preliminary evaluation of turbidity removal
by natural coagulants indigenous to Venezuela. Process
Biochem. 35 (3–4), 391–395.
.ac
Doll, S.R., 1993. Review: Alzheimer's disease and environmental
aluminium. Age Ageing 22 (2), 138–153.
sc
je
2188 J O U R NA L OF EN V I RO N M EN T A L S CI EN C ES 2 6 ( 20 1 4 ) 2 1 78–2 1 8 9
Dorea, C.C., 2006. Use of Moringa spp. seeds for coagulation: a
review of a sustainable option. Water Sci. Technol. Water
Supply 6 (1), 219–227.
Driscoll, C.T., Letterman, R.D., 1995. Factors regulating residual
aluminium concentrations in treated waters. Environmetrics 6
(3), 287–309.
Duan, J., Gregory, J., 2003. Coagulation by hydrolysing metal salts.
Adv. Colloid Interf. Sci. 100–102, 475–502.
Dubey, D., Balamurugan, K., Agrawal, R.C., Verma, R., Jain, R., 2011.
Evalution of antibacterial and antioxidant activity of
methanolic and hydromethanolic extract of sweet orange
peels. Recent Res. Sci. Technol. 3 (11), 22–25.
Egila, J., Dauda, B., Iyaka, Y., Jimoh, T., 2011. Agricultural waste as
a low cost adsorbent for heavy metal removal from
wastewater. Int. J. Phys. Sci. 6 (8), 2152–2157.
Ejelonu, B.C., Oderinde, R.A., Balogun, S.A., 2010. Chemical and
biological properties of Jatropha curcas and Mucuna solan seed
and seed oil. Libyan Agric. Res. Center J. Int. 1 (4), 263–268.
Engels, C., Knödler, M., Zhao, Y.Y., Carle, R., Gänzle, M.G., Schieber,
A., 2009. Antimicrobial activity of gallotannins isolated from
mango (Mangifera indica L.) kernels. J. Agric. Food Chem. 57
(17), 7712–7718.
FAO, 1970. Tannin (food grade). FAO Nutrition Meetings Report
(Geneva).
FAO, 2012. Food Outlook: Global Market Analysis. FAO, (www.fao.
org. Accessed 19 February 2013).
Fatombi, J.K., Lartiges, B., Aminou, T., Barres, O., Caillet, C., 2013. A
natural coagulant protein from copra (Cocos nucifera): isolation,
characterization, and potential for water purification. Sep.
Purif. Technol. 116, 35–40.
Flaten, T.P., 2001. Aluminium as a risk factor in Alzheimer's disease,
with emphasis on drinking water. Brain Res. Bull. 55 (2), 187–196.
Forster, D.P., Newens, A.J., Kay, D.W.K., Edwardson, J.A., 1995. Risk
factors in clinically diagnosed presenile dementia of the
Alzheimer type: a case–control study in northern England. J.
Epidemiol. Community Health 49 (3), 253–258.
Fuentes, S.L.C., Mendoza, S.I.A., López, M.A.M., Castro, V.M.F.,
Urdaneta, M.C.J., 2011. Effectiveness of a coagulant extracted
from Stenocereus griseus (Haw.) Buxb in water purification. Rev.
Téc. Ing. Univ. Zulia 34 (1), 48–56.
Gauthier, E., Fortier, I., Courchesne, F., Pepin, P., Mortimer, J.,
Gauvreau, D., 2000. Aluminum forms in drinking water and
risk of Alzheimer's disease. Environ. Res. 84 (3), 234–246.
Gebbie, P., 2005. A dummy's guide to coagulants. Proc. of the 68th
Annual Water Industry Engineers, Operators' Conference.
Bendigo, Australia.
Ghebremichael, K., 2007. Overcoming the drawbacks of natural
coagulants for drinking water treatment. Water Sci. Technol.
Water Supply 7 (4), 87–93.
Ghebremichael, K.A., Gunaratna, K.R., Henriksson, H., Brumer, H.,
Dalhammar, G., 2005. A simple purification and activity assay
of the coagulant protein from Moringa oleifera seed. Water Res.
39 (11), 2338–2344.
Göttsch, E., 1992. Purification of turbid surface water by plants in
Ethiopia. Walia 14, 23–28.
Gregory, J., 2006. Floc formation and floc structure. In: Newcombe,
G., Dixon, D. (Eds.), Interface Science in Drinking Water
Treatment vol. 3. Academic Press, UK, pp. 26–43.
Gruninger, R.M., 1975. Disposal of waste alum sludge from water
treatment plants. Water Pollut. Control Fed. 47 (3), 543–552.
Gunaratna, K.R., Garcia, B., Andersson, S., Dalhammar, G., 2007.
Screening and evaluation of natural coagulants for water
treatment. Water Sci. Technol. Water Supply 7 (5–6), 19–25.
Hacker, J., Mattingly, J., Caputo, J., 2009. Food and Crop Waste: A
Valuable Biomass. Environmental and Energy Study Institute,
Washington, DC.
Idris, J., Md Som, A., Musa, M., Ku Hamid, K.H., Husen, R., Muhd
Rodhi, M.N., 2013. Dragon fruit foliage plant-based coagulant
for treatment of concentrated latex effluent: comparison of
treatment with ferric sulfate. J. Chem. http://dx.doi.org/10.
1155/2013/230860 (Article ID 230860).
Ikewuchi, C.C., Ikewuchi, J.C., 2008. Chemical profile of Pleurotus
tuberregium (Fr) Sing's sclerotia. Pac. J. Sci. Technol. 10 (1),
295–299.
Jahn, S.A.A., 2001. Drinking water from Chinese rivers: challenges
of clarification. J. Water Supply Res Technol. 50 (1), 15–27.
Karawya, M.S., Wassel, G.M., Baghdadi, H.H., Ammar, N.M., 1980.
Mucilages and pectins of Opuntia, Tamarindus and Cydonia. J.
Med. Plant Res. 40, 68–75.
Li, T., Zhu, Z., Wang, D.S., Yao, C.H., Tang, H.X., 2006.
Characterization of floc size, strength and structure under
various coagulation mechanisms. Powder Technol. 168 (2),
104–110.
Mallikharjuna, P.B., Rajanna, L.N., Seetharam, Y.N.,
Sharanabasappa, G.K., 2007. Phytochemical studies of
Strychnos potatorum Lf—a medicinal plant. J. Chem. 4 (4),
510–518.
Mane, P.C., Bhosle, A.B., Jangam, C.M., Mukate, S.V., 2011.
Heavy metal removal from aqueous solution by Opuntia:
a natural polyelectrolyte. J. Nat. Prod. Plant Resour. 1 (1),
75–80.
Marobhe, N.J., Dalhammar, G., Gunaratna, K.R., 2007. Simple and
rapid methods for purification and characterization of active
coagulants from the seeds of Vigna unguiculata and Parkinsonia
aculeata. Environ. Technol. 28 (6), 671–681.
Martine, G., 2007. State of World Population 2007: Unleashing the
Potential of Urban Growth, (New York).
Martyn, C.N., Coggon, D.N., Inskip, H., Lacey, R.F., Young, W.F.,
1997. Aluminum concentrations in drinking water and risk of
Alzheimer's disease. Epidemiology 8 (3), 281–286.
Mavura, W.J., Chemelil, M.C., Saenyi, W.W., Mavura, H.K., 2008.
Investigation of chemical and biochemical properties of
Maerua subcordata plant extract: a local water clarification
agent. Bull. Chem. Soc. Ethiop. 22 (1), 143–148.
McLachlan, D.R., 1995. Aluminium and the risk for Alzheimer's
disease. Environmetrics 6 (3), 233–275.
Miller, S.M., Fugate, E.J., Craver, V.O., Smith, J.A., Zimmerman, J.B.,
2008. Toward understanding the efficacy and mechanism of
Opuntia spp. as a natural coagulant for potential application in
water treatment. Environ. Sci. Technol. 42 (12), 4274–4279.
Mirhosseini, H., Amid, B.T., 2012. A review study on chemical
composition and molecular structure of newly plant gum
exudates and seed gums. Food Res. Int. 46 (1), 387–398.
Mishra, A., Agarwal, M., Yadav, A., 2003. Fenugreek mucilage as a
flocculating agent for sewage treatment. Colloid Polym. Sci.
281 (2), 164–167.
Nasrabadi, T., Abbasi Maedeh, P., 2014. Groundwater quality
degradation of urban areas (case study: Tehran city, Iran). Int.
J. Environ. Sci. Technol. 11 (2), 293–302.
Ndabigengesere, A., Subba Narasiah, K., Talbot, B.G., 1995. Active
agents and mechanism of coagulation of turbid waters using
Moringa oleifera. Water Res. 29 (2), 703–710.
Nurika, I., Mulyarto, A.R., Afshari, K., 2007. Utilization of tamarind
(Tamarindus indica) seed for coagulation of tofu industry
wastewater (study on tamarind seed powder concentration
and stirring time). J. Teknol. Pert. 8 (3), 215–220.
Oko, A.O., Ugwu, S.I., 2011. The proximate and mineral
compositions of five major rice varieties in Abakaliki,
South-Eastern Nigeria. Int. J. Plant Physiol. Biochem. 3 (2),
25–27.
Okuda, T., Baes, A.U., Nishijima, W., Okada, M., 2001. Isolation and
characterization of coagulant extracted from Moringa oleifera
seed by salt solution. Water Res. 35 (2), 405–410.
.cn
Osato, J.A., Santiago, L.A., Remo, G.M., Cuadra, M.S., Mori, A., 1993.
Antimicrobial and antioxidant activities of unripe papaya. Life
Sci. 53 (17), 1383–1389.
.ac
Perveen, K., Bokhari, N.A., Soliman, D.A.W., 2012. Antibacterial
activity of Phoenix dactylifera L. leaf and pit extracts against
sc
je
 JO U R N A L OF EN V I RO N M EN T A L S CI E NC ES 2 6 ( 20 1 4 ) 2 1 7 8–2 1 8 9
selected Gram negative and Gram positive pathogenic
bacteria. J. Med. Plants Res. 6 (2), 296–300.
Pritchard, M., Mkandawire, T., Edmondson, A., O'neill, J.G.,
Kululanga, G., 2009. Potential of using plant extracts for
purification of shallow well water in Malawi. Phys. Chem.
Earth 34 (13–16), 799–805.
Qureshi, K., Bhatti, I., Shaikh, M.S., 2011. Development of
bio-coagulant from mango pit for the purification of turbid
water. Sind. Univ. Res. J. (Sci. Ser.) 43 (1), 105–110.
Raghuwanshi, P.K., Mandloi, M., Sharma, A.J., Malviya, H.S.,
Chaudhari, S., 2002. Improving filtrate quality using agrobased
materials as coagulant aid. Water Qual. Res. J. Can. 37 (4),
745–756.
Ramamurthy, C., Maheswari, M.U., Selvaganabathy, N., Kumar,
M.S., Sujatha, V., Thirunavukkarasu, C., 2012. Evaluation of
eco-friendly coagulant from Trigonella foenum-graecum seed.
Adv. Biol. Chem. 2 (1), 58–63.
Rondeau, V., Commenges, D., Jacqmin-Gadda, H., Dartigues, J.F.,
2000. Relation between aluminum concentrations in drinking
water and Alzheimer's disease: an 8-year follow-up study. Am.
J. Epidemiol. 152 (1), 59–66.
Rondeau, V., Jacqmin-Gadda, H., Commenges, D., Dartigues, J.F.,
2001. Re: aluminium in drinking water and cognitive decline in
elderly subjects: the Paquid cohort. Am. J. Epidemiol. 154 (3),
288–290.
Ruijter, A. de, 2008. Strychnos potatorum L.f. In: Schmelzer, G.H.,
Fakim, A.G. (Eds.), Plant Resources of Tropical Africa 11 (1).
Medicinal Plants 1. PROTA Foundation/Backhuys Publishers/
CTA, Wageningen, Netherlands.
Sathiyabama, M., 2012. Purification of a coagulant protein from
seeds of Moringa concanensis. Water Sci. Technol. Water Supply
12 (3), 329–333.
Servyeco Group, 2008. Natural plant coagulants for wastewater
treatment: natural plant based coagulants derived from Acacia
for wastewater treatment, series ECOTAN. http://servyeco.
com/530055_en/Natural-coagulants-for-wastewater-
treatment-plant/ (Accessed 1 November 2012).
Shilpa, B.S., Akanksha, K., Girish, P., 2012. Evaluation of cactus and
hyacinth bean peels as natural coagulants. Int. J. Chem.
Environ. Eng. 3 (3), 187–191.
Simate, G.S., Iyuke, S.E., Ndlovu, S., Heydenrych, M., Walubita, L.F.,
2012. Human health effects of residual carbon nanotubes and
traditional water treatment chemicals in drinking water.
Environ. Int. 39 (1), 38–49.
Sobukola, O.P., Abayomi, H.T., 2011. Physical properties and
rehydration characteristics of different varieties of maize (Zea
mays L.) and cowpea (Vigna unguiculata L. Walp) seeds. J. Food
Process. Preserv. 35 (3), 299–307.
Som, A.M., Idris, J., Hamid, K.H.K., 2007. Dragon fruit foliage as a
low cost plant-based coagulant in latex concentrate
wastewater treatment. Malay. J. Chem. Eng. 49–59.
Sowmeyan, R., Santhosh, J., Latha, R., 2011. Effectiveness of herbs
in community water treatment. Int. Res. J. Biochem.
Bioinforma. 1 (11), 297–303.
Subbaramiah, K., Rao, B.S., 1937. The mechanisms of the
clarification of muddy water by Strychnos potatorum seeds.
Proceedings of the Indian Academy of Sciences. Indian
Academy of Sciences, pp. 59–70.
Subramanium, S., Nand, V., Maata, M., Koshy, K., 2011. Moringa
oleifera and other local seeds in water purification in
developing countries. Res. J. Chem. Environ. 15 (2), 135–138.
2189
Sutherland, J., Folklard, G., Poirier, Y., 2001. Moringa oleifera. The
constraints to commercialization. International Workshop:
29th October–2nd November, Dar es Salaam, Tanzania, 2001.
Swati, M., Govindan, V.S., 2005. Coagulation studies on natural
seed extracts. J. Indian Water Works Assoc. 37 (2), 145–149.
Thakre, V.B., Bhole, A.G., 1985. Relative evaluation of a few natural
coagulants. J. Inst. Eng. (India) Environ. Eng. Div. 65, 89–92.
Tripathi, P.N., Chaudhuri, M., Bokil, S.D., 1976. Nirmali seed—a
naturally occurring coagulant. Indian J. Environ. Health 18 (4),
272–281.
United States Environmental Protection Agency, 2006.
Reassessment of One Exemption From the Requirement of a
Tolerance for Tannin (CAS Reg. No. 1401-55-4), (Washington,
DC).
Vijayaraghavan, G., Sivakumar, T., Vimal Kumar, A., 2011.
Application of plant based coagulants for waste water
treatment. Int. J. Adv. Eng. Res. Stud. 1 (1), 88–92.
Walton, J.R., 2013. Aluminum's involvement in the progression of
Alzheimer's disease. J. Alzheimer's Dis. 35 (1), 7–43.
Wettstein, A., Aeppli, J., Gautschi, K., Peters, M., 1991. Failure to
find a relationship between mnestic skills of octogenarians
and aluminum in drinking water. Int. Arch. Occup. Environ.
Health 63 (2), 97–103.
WHO, 2011. Guidelines for Drinking-water Quality, 4th ed.,
(Geneva).
World Water Assessment Programme, 2009. The United Nations
World Water Development Report 3: Water in a Changing
World. UNESCO, Paris.
Wu, T.Y., Mohammad, A.W., Lim, S.L., Lim, P.N., Hay, J.X.W., 2013.
Recent advances in the reuse of wastewaters for promoting
sustainable development. In: Sharma, S.K., Sanghi, R. (Eds.),
Wastewater Reuse and Management. Springer, Netherlands,
pp. 47–103.
Yang, Y.C., Abdul-Talib, S., Pei, L.Y., Ismail, M.S.N., Abd-Razak,
S.N.A., Mohd-Mohtar, A.M., 2007. A study on cactus Opuntia
as natural coagulant in turbid water treatment. CSSR 6, 1–7.
Yiğit, D., Yiğit, N., Mavi, A., 2009. Antioxidant and antimicrobial
activities of bitter and sweet apricot (Prunus armeniaca L.)
kernels. Braz. J. Med. Biol. Res. 42 (4), 346–352.
Yin, C.Y., 2010. Emerging usage of plant-based coagulants for
water and wastewater treatment. Process Biochem. 45 (9),
1437–1444.
Yongabi, K.A., 2006. Studies on the potential use of medicinal
plants and macro-fungi (lower plants) in water and waste
water purification. Tree Life J. 10, 1–14.
Yongabi, K.A., 2010. Biocoagulants for water and waste water
purification: a review. Int. Rev. Chem. Eng. 2 (3), 444–458.
Yongabi, K.A., Lewis, D.M., Harris, P.L., 2011a. Application of
phytodisinfectants in water purification in rural Cameroon.
Afr. J. Microbiol. Res. 5 (6), 628–635.
Yongabi, K.A., Lewis, D.M., Harris, P.L., 2011b. Indigenous plant
based coagulants/disinfectants and sand filter media for
surface water treatment in Bamenda, Cameroon. Afr. J.
Biotechnol. 10 (43), 8625–8629.
Zhang, J.D., Zhang, F., Luo, Y.H., Yang, H., 2006. A preliminary
study on cactus as coagulant in water treatment. Process
Biochem. 41 (3), 730–733.
.cn
.ac
sc
je
 Editorial Board of Journal of Environmental Sciences
Editor-in-Chief
Hongxiao Tang Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, China

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Shu Tao Peking University, China
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Aquatic environment Michael Schloter Environmental toxicology and health
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University of Wyoming, USA Peking University, China Peking University, China
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National Chiao Tung University Zhejiang University, China Research Center for Eco-Environmental Sciences,
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