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Now, some of you have done strawberries before. So, today you will do strawberries as well as an alternative
food choice. Please discuss with your lab group and determine this AHEAD OF TIME. If you give me enough
notice, I can purchase this, or you may bring from home.
Take a look at the sketch of the plant cell below. The chromosomes (which
are made of DNA) are in the nucleus. This is the place where most of the
DNA is located. (There are also small amounts of DNA in the chloroplast and
mitochondria.)
4. What do you think the DNA will look like when you extract it from the
plant cell? (Write a brief description.)
5. List the order of organization from DNA to chromosome. **You will
need to get this from me because it is in the notes that we have not
covered yet.
Write a Hypothesis: What do you think DNA will look like when it is extracted from the strawberry? Why?
Draw your strawberry and alternative food choice prior to starting the lab! This goes down as observational notes.
Materials:
Procedure:
Working together in your pairs, read through the entire procedure & follow the steps below.
REPEAT STEPS 1-8 with your alternative food choice as well. You can have both going on the same time if there are
enough materials.
As you wait for your solution to filter, complete the following questions:
Part I: Questions
Watch where the alcohol and extract layers come in contact with each other. Keep the tube at eye level so you
can see what is happening.
What do you see appearing? (Sketch what you see and note any other observations.)
1. It is important that you understand the steps in the extraction procedure and why each step was necessary. Each
step in the procedure aided in isolating the DNA from other cellular materials. Match the procedure with its
function:
PROCEDURE FUNCTION
A. Filter strawberry slurry through coffee filter ___ To precipitate DNA from solution
B. Mush strawberry with salty/soapy solution ___ Separate components of the cell
C. Initial smashing and grinding of strawberry ___ Break open the cells
D. Addition of ethanol to filtered extract ___ Break up proteins and dissolve cell membranes