Group #8 Section : 8 – Humility Date Performed: 8/13/19

Members: Date Submitted: 8/23/19

Rubin, Khloe
Dytioco, Jason
Tabernilla, Jorel
Samra, Sahibjot

Experiment #2
“Miniature World of Plants and Animals”

I. Objectives
1. Prepare specimen slides of onion skin cells and human cheek cells.
2. Differentiate plant cells from animal cells.

II. Estimated Time Frame

- Examining Plant Cells: 40-50 minutes
- Examining Animal Cells: 40-50 minutes
III. Materials
Glass slides Mounting Needle Scalpel
Coverslips Filter Paper Fresh Onion
Compound Microscope Clean Toothpick Diluted Iodine Solution or Methylene
Dropper Forceps Blue Solution

IV. Procedure
1. Cut a fresh onion into two and take out a piece from the outer purple scale lead of the
onion bulb. Using forceps, peel off a thin, white layer of the onion skin from the inside of
the scale leaf.
2. Lay the onion skin on a clean glass slide as flat as possible. Add a drop of iodine solution
or methylene blue solution on the onion skin. Be careful when working with stains.
Avoid contact with skin, clothing, or table surfaces.
3. Carefully place a coverslip from the side of the specimen. Lowering it gently to avoid
trapping air bubbles.
4. Wipe off the excess stains around the coverslip with filter paper.
5. Use the microscope to observe your prepared specimen slide under LPO, and the under
HPO.
6. Draw the plant cells and label the parts that you can see under the microscope. Indicate
the magnification.
B. Examining Animal Cells
1. Use the end of a clean toothpick to gently scrape some cells from the inside of your
cheek.
2. Smear the scrapings onto a glass slide to allow them to dry. Be sure to throw away the
toothpick after use. Do not share it with your groupmates.
3.Place a drop of iodine or methylene blue solution on the smear and leave it for one to two
minutes. Be careful when working with stains. Avoid contact with skin, clothing, or table
surfaces.
4. Mount the cells on the glass slide. Carefully lay one side of the coverslip close to the drop
of the solution, while supporting the other side of the coverslip with the mounting needle.
Gently and lowly lower the coverslip over the specimen to avoid trapping air bubbles.
5. Wipe off the excess stain around the coverslip with filter paper.
6. Observe your cheek cells under the microscope. Move the slide to find a good spot were
the cells do not clump or overlap. Examine your specimen slide under the LPO, and the
under HPO.
7. Draw the animal cell and label the parts that you can see under the microscope. Indicate
the magnification.

V. Illustrations.
 Onion skin cells under LPO
LPO (Magnification: 10x) HPO (Magnification: 40x)

Cheek cells under LPO

LPO (Magnification: 10x) HPO (Magnification: 40x)

VI. Conclusion:
We conclude that cells can be seen in the microscope and that proves that his findings were
correct because cells can be seen as monk quarters.

VII. Post-Laboratory Questions

1. What structures were you able to observe in both the prepares slides? What structures were
you able to observe in onion skin cells that were not found in human cheek cells?
A: There were like rooms/monk quarters, but the cheek cell has only one. We observed that they are not
the same in structures, but they are both cells.

2. Are all human cheek cells the same or different in structure from one another? Are all onion skin cells
the same or different in structure from another? Give a reason for your answer.
A: The cheek cells are different in structure. The onion skin cells are also different in structure because,
the onion Is different, some onions are white while some are purple.

3. Can you clearly see the cell membrane n the human cheek cells and the cell wall in the onion skin
cells? How do you determine their location in the cell? Describe their shape and appearance.

A: For us, you can only see the structure of it but cannot see clearly the specific parts. The Human cheek
cells only composes an oval/circle structure and inside it is another circle structure.

4. What did you observe about the nucleus in both the onion skin cell and the human cheek cells?
Describe its shape, color, and location.

A: For the onion cell, we saw box and circle shapes, while for the cheek cell we saw a circle and around it
is a big circle shape. The onion cell is color violet/red. While the cheek cell is white.

5. How many cells were you able to mount from your inner cheek cell scrapings? What can you infer
from this observation?

A: There are too many cells that can be found in the inner cheek.