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Aim: To investigate a new range of Pepsi Cola drinks: “Pepsi Mix”, “Pepsi Max”, “Pepsi Mox”.
Your task is to find out which sugar is used in each drink.
Introduction
You are working as a biochemist for a rival soft drinks company
and you need to find out which sugars are used in the new
recipies for three Pepsi products.
Hypothesis
The sugar could be glucose (a monosaccharide found in all cells), maltose (a disaccharide extracted
from seeds), sucrose (disaccharide, the sugar found in plant stems and cane sugar) or starch (a
polysaccharide found in plants). Two simple food tests can be used to find out. Benedict's Reagent
to test for glucose (reducing sugars) and Iodine to test for starch.
Starch has no effect on Benedict's reagent because all the reducing groups are used to hold the chain
of glucose molecules together. It does change the colour of iodine. None of the other carbohydrates
cause the iodine to change colour from brown to "blue-black".
Apparatus
Each group of students will need the following apparatus:
Safety
Dropping pipettes Wear eye protection
Measuring cylinder
Benedict's Reagent (dropping bottle) Tie back long hair
Iodine Solution (dropping bottle)
Water bath set to 80°C
Stopwatch
Dilute solutions of sucrose, glucose, starch, maltose (for testing)
Sample of Pepsi MIX, 100ml
Sample of Pepsi MAX 100ml
Sample of Pepsi MOX 100ml
(see teacher’s notes for “Pepsi” recipes)
Compare the drink samples with the sugar samples. Explain any similarities and differences.