BIOTECHNOLOGY

MULTIPLE CHOICE
QUESTIONS
 APPLICATIONS
OF
BIOTECHNOLOGY

IN AGRICULTURE & IN MEDICINE

In this section we have studied
the following:
 Genetically modified crop
plants
 Genetically modified animals
 Biofortification
 Molecular diagnosis
 Gene therapy
 Gene manipulation through
interference RNA
What is not true about the
genetically modified ‘Bt crops’?
1) The technique has been applied
to several species of crops
2) The source organism for the gene
is a soil bacterium
3) The transferred gene exists in
several allelic forms
4) Bt brinjal has been commercially
exploited
What is not true about the
genetically modified ‘Bt crops’?
1) The technique has been applied
to several species of crops
2) The source organism for the gene
is a soil bacterium
3) The transferred gene exists in
several allelic forms
4) Bt brinjal has been commercially
exploited
Golden rice is a genetically
modified crop plant where the
incorporated genes are meant
for biosynthesis of -------.

1) vitamin A
2) vitamin C
3) vitamin B
4) Beta-carotene
Golden rice is a genetically
modified crop plant where the
incorporated genes are meant
for biosynthesis of -------.

1) vitamin A
2) vitamin C
3) vitamin B
4) Beta-carotene
Which organism among the following
was not used in the genetic
engineering programme leading to
the development of Golden Rice?

1) Escherichia coli
2) Erwinia uredovora
3) Agrobacterium tumefaciens
4) Narcissus pseudonarcissus
Which organism among the following
was not used in the genetic
engineering programme leading to
the development of Golden Rice?

1) Escherichia coli
2) Erwinia uredovora
3) Agrobacterium tumefaciens
4) Narcissus pseudonarcissus
------- is the product of RNAi
technique in genetic engineering.
1) Cry proteins of Bt cotton

2) Cry proteins of Bt brinjal

3) Beta carotene enriched Golden

rice

4) Nematode resistance in tobacco

------- is the product of RNAi
technique in genetic engineering.
1) Cry proteins of Bt cotton

2) Cry proteins of Bt brinjal

3) Beta carotene enriched Golden

rice

4) Nematode resistance in tobacco

Interference RNA was discovered by

1) Cohen & Boyer

2) Fire & Mello
3) Yonath & Ramakrishnan
4) Holley & Khorana
Interference RNA was discovered by

1) Cohen & Boyer

2) Fire & Mello
3) Yonath & Ramakrishnan
4) Holley & Khorana
Identify the organism in which RNAi
was first discovered.

1) Meloidegyne incognitia
2) Escherichia coli
3) Bacillus thuringiensis
4) Coenorhabditis elegans
Identify the organism in which RNAi
was first discovered.

1) Meloidegyne incognitia
2) Escherichia coli
3) Bacillus thuringiensis
4) Coenorhabditis elegans
The function of Interference RNA to
prevent a gene from functioning is
also known as
1) Reverse transcription
2) Epigenetics
3) PTGS (Post Transcriptional
Gene Silencing)
4) RNA splicing
The function of Interference RNA to
prevent a gene from functioning is
also known as
1) Reverse transcription
2) Epigenetics
3) PTGS (Post Transcriptional
Gene Silencing)
4) RNA splicing
This is not a product of
recombinant DNA technology:

1) Golden rice
2) Tracy
3) Bt cotton
4) Dolly
This is not a product of
recombinant DNA technology:

1) Golden rice
2) Tracy
3) Bt cotton
4) Dolly
 This is not a GMO.

1) Bt cotton
2) Golden rice
3) Tracy
4) Pomato
 This is not a GMO.

1) Bt cotton
2) Golden rice
3) Tracy
4) Pomato
In the famous rDNA experiment to
produce humulin, the substance
was produced attached with
another protein called -------.

1) beta-galactosidase
2) beta-galactoside
3) ampicillin
4) tetracycline
In the famous rDNA experiment to
produce humulin, the substance
was produced attached with
another protein called -------.

1) beta-galactosidase
2) beta-galactoside
3) ampicillin
4) tetracycline
In the famous rDNA experiment to
produce humulin, the substance
was produced attached with
another protein called -------.

1) beta-galactosidase
2) beta-galactoside
3) ampicillin
4) tetracycline
The active form of human insulin
consists of these segments of
polypeptide chains.

1) A & B Chains
2) A & C Chains
3) B & C Chains
4) A, B & C Chains
The active form of human insulin
consists of these segments of
polypeptide chains.

1) A & B Chains
2) A & C Chains
3) B & C Chains
4) A, B & C Chains
In the famous example of human
gene therapy for SCID disease,
the procedure adopted was:

1) ex vivo germ cell therapy

2) ex vivo somatic cell therapy
3) in vivo germ cell therapy
4) in vivo somatic cell therapy
In the famous example of human
gene therapy for SCID disease,
the procedure adopted was:

1) ex vivo germ cell therapy

2) ex vivo somatic cell therapy
3) in vivo germ cell therapy
4) in vivo somatic cell therapy
What is the advantage in clinical use
of humulin (human insulin produced
through rDNA technique) over use of
conventional ox or pig insulin?
1) It does not cause immunological
problems
2) It is cheaper for the patient
3) It is produced by E.coli in our
own intestine
4) There is no advantage
What is the advantage in clinical use
of humulin (human insulin produced
through rDNA technique) over use of
conventional ox or pig insulin?
1) It does not cause immunological
problems
2) It is cheaper for the patient
3) It is produced by E.coli in our
own intestine
4) There is no advantage
Gene therapy for SCID disease in
man involves the transfer of the
gene for this enzyme

1) Beta-galactosidase
2) Thrombokinase
3) Adenosine deaminase
4) Phenylalanine hydroxylase
Gene therapy for SCID disease in
man involves the transfer of the
gene for this enzyme

1) Beta-galactosidase
2) Thrombokinase
3) Adenosine deaminase
4) Phenylalanine hydroxylase
Spot the mismatch between the
organisms with the actual
modifications done:
1) Rosie – Lactalbumin enriched
milk
2) Tracy – Human AAT protein
enriched milk
3) GM Mice - Cry protein
enrichment
4) Golden rice – beta-carotene
enrichment
Spot the mismatch between the
organisms with the actual
modifications done:
1) Rosie – Lactalbumin enriched
milk
2) Tracy – Human AAT protein
enriched milk
3) GM Mice - Cry protein
enrichment
4) Golden rice – beta-carotene
enrichment
One of the advantages of
developing transgenic mice is it
is very useful in:

1) Keeping mice population under

control
2) Producing new varieties of mice
3) Developing a show piece
example
4) Gene targeting
One of the advantages of
developing transgenic mice is it
is very useful in:

1) Keeping mice population under

control
2) Producing new varieties of mice
3) Developing a show piece
example
4) Gene targeting
When these parts of a plant are
used as explants in a tissue culture
experiments the newly generated
plants could be virus-free.

1) Pollen grains
2) Meristems
3) Parts of the embryo
4) Flower buds
When these parts of a plant are
used as explants in a tissue culture
experiments the newly generated
plants could be virus-free.

1) Pollen grains
2) Meristems
3) Parts of the embryo
4) Flower buds
Haploid plants could be produced
from tissue culture of the following
parts of a healthy plant:

1) Pollen grains
2) Meristems
3) Parts of the embryo
4) Flower buds
Haploid plants could be produced
from tissue culture of the following
parts of a healthy plant:

1) Pollen grains
2) Meristems
3) Parts of the embryo
4) Flower buds
Normally this is not expected in the
new plants produced through
tissue culture programmes:

1) Vigorous growth
2) Quicker maturity
3) Formation of embryoids
4) Variations in genotype
Normally this is not expected in the
new plants produced through
tissue culture programmes:

1) Vigorous growth
2) Quicker maturity
3) Formation of embryoids
4) Variations in genotype
Tissue culture experiments have to be
conducted in extremely aseptic
conditions. Therefore all things used in
the tissue culture programme must be
properly sterilized before use with the
exception of --------------.

1) The explant
2) The culture vessel
3) The various instruments used
4) The nutrient medium
Tissue culture experiments have to be
conducted in extremely aseptic
conditions. Therefore all things used in
the tissue culture programme must be
properly sterilized before use with the
exception of --------------.

1) The explant
2) The culture vessel
3) The various instruments used
4) The nutrient medium
 THANK YOU

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shivashankarblore@gmail.com