CONCENTRACIÓN

Muestras
CONCENTRACION DE GLUCOSA
CONCENTRACION
CONTROL DE GLUCOSA SAL CONTROL (μg/ml)
0.202 0.197 1 387.67
0.173 0.14 2 339.33
0.181 0.167 3 352.67
0.16 0.171 4 317.67
0.183 0.16 5 356
0.138 0.146 6 281
0.142 0.145 7 287.67
0.141 0.143 8 286
0.145 0.172 9 292.67
0.171 0.159 10 336
0.153 0.162 11 306
0.131 0.128 12 269.33
0.272 0.271 13 504.33
0.142 0.162 14 287.67
0.13 0.132 15 267.67
 CONCENTRACIÓN MUESTRA CONTROL
ESTRÉS SALINO (μg/mL) TALLO RAIZ ENVERGADURA TALLO RAIZ ENVERGADURA
379.33 6 7.2 5.5 8 7.5 3.5
284.33 9 6.5 5 8.5 9 6.5
329.33 6.5 16.8 4 6.7 16.7 4.5
336 5.8 16.5 3 6 16 3.5
317.667 4.6 3.3 1 4.4 4.7 1.8
294.33 4.6 4.5 1.5 4.8 3.6 2
292.67 3.5 4.3 2 2.9 2.7 1.4
289.33 4.2 3.3 15 3.2 3.4 0.3
337.67 4 4.3 0.5 2.8 2.2 0.4
316 6.5 7.5 2 9 7 1.5
321 8 10 1.5 9 10 2
264.33 8 8 2 9 10.5 2
502.67 21 28.5 8 25 18.5 10
321 6.7 11 2 9.2 8 3
271 13 10.6 6.1 12 10.5 5.2
18 15 7.8 20.2 16 7.6
8.1 2.5 0.5 7.6 2.5 0.8
6.6 7.9 0.5 6.9 6.8 0.7
8 11.5 0.5 4.5 5.2 0.5
5 8.6 0.4 5 1.7 1.8
5.5 14.5 0.6 4.5 1.1 1
3 7 3 5.5 10.2 1
7 1.5 1 6 2 0.5
5.2 1.4 1.5 9.5 4 1
5.5 1.5 0.6 4.5 2.3 0.7
4.8 1 0.5 3.8 1.7 1
11 4.5 14 23 9 12.8
9 5.5 14 19 7.3 14.8
4.7 4.5 2 11.1 7 2.5
19 8 24 18 19 23
CONCENTRACION DE CONCENTRACION DE
PROTEINAS MUESTRA PROTEINAS CONTROL MUESTRAS CONCENTRACION
(mg/ml) (mg/ml) CONTROL (ug/ml)
0.113 0.122 1 2090
0.039 0.07 2 1032.86
0.088 0.098 3 1732.87
0.071 0.1 4 1490
0.235 0.185 5 3832.86
0.206 0.195 6 3418.57
0.095 0.041 7 1832.86
0.142 0.117 8 2504.29
0.081 0.056 9 1632.86
0.245 0.213 10 3975.71
0.114 0.117 11 2104.29
0.087 0.097 12 1718.57
0.064 0.024 13 1390
0.056 0.156 14 1275.71
0.141 0.074 15 2490
0.069 0.083 16 1461.43
 MUESTRA CONTROL

CONCENTRACION
ESTRÉS SALINO (ug/ml) TALLO RAIZ ENVERGADURA TALLO RAIZ ENVERGADURA
2218.57 7.3 6.5 3.4 17.5 10 8.5
1475.71 5 7.9 3 6.9 16.9 5.5
1875.71 6.4 16.3 5 3 6 1.5
1904.29 3.6 4 0.9 4.5 3.3 1
3118.57 4 2.9 0.5 2.6 3 2
3261.43 4.2 3 1.6 4 5 2
1061.43 3.2 3.2 2.2
2147.14 8.5 14 2.5 18 16 16
1275.71 7.5 11.5 1.5 14.5 20 3
3518.57 4.2 3 1.8 4 3.2 1.5
2147.14 58.5 57.5 12 32 38 11.5
1861.43 20 16 8.5 22 18 8.3
818.57 10.8 2.7 0.6 10.6 2.7 0.8
2704.29 7.8 9.5 1.2 8 6.7 1
1532.86 8.5 8 3.8 6.5 12.5 4
1661.43 7.5 0.5 0.3 13 2 3.2
5 2.5 0.6 10.8 2.4 2.5
5.1 2.2 1 5.7 1.8 1.5
6.5 1.6 0.8
18 19 23 19 8.5 24
4.7 4.5 2 11.1 7 2.5
mg/uL

1000 0.04
2000 0.1
3000 0.18

Curva de calibración de proteínas totales

0.2
0.18
0.16 f(x) = 0.00007x - 0.0333333333

0.14
Absorbancias

0.12
0.1
0.08
0.06
0.04
0.02
0
500 1000 1500 2000 2500 3000 3500

Concentración de las proteínas (ug/ml)

 PROTEINAS
CONCENTRA
CONCENTRA CIÓN
CIÓN
Muestras ESTRÉS
CONTROL
(μg/ml) SALINO
MUESTRA PROBLEMA CONTROL (μg/mL)
2090.4285714286 2219 C 387.67 379.33
1033.2857142857 1476.142857143 C 339.33 284.33
1733.2857142857 1876.142857143 C 352.67 329.33
1490.4285714286 1904.714285714 C 317.67 336
3833.2857142857 3119 C 356 317.667
3419 3261.857142857 C 281 294.33
1833.2857142857 1061.857142857 C 287.67 292.67
2504.7142857143 2147.571428571 C 286 289.33
1633.2857142857 1276.142857143 C 292.67 337.67
3976.1428571429 3519 C 336 316
2104.7142857143 2147.571428571 C 306 321
1719 1861.857142857 C 269.33 264.33
1390.4285714286 819 C 504.33 502.67
1276.1428571429 2704.714285714 C 287.67 321
2490.4285714286 1533.285714286 C 267.67 271
1461.8571428572 1661.857142857
476.1428571429 476.1428571429
 MUESTRA CONTROL

TALLO RAIZ ENVERGADU TALLO RAIZ

7.3 6.5 3.4 17.5 10

5 7.9 3 6.9 16.9
6.4 16.3 5 3 6
3.6 4 0.9 4.5 3.3
4 2.9 0.5 2.6 3
4.2 3 1.6 4 5
3.2 3.2 2.2
8.5 14 2.5 18 16
7.5 11.5 1.5 14.5 20
4.2 3 1.8 4 3.2
58.5 57.5 12 32 38
20 16 8.5 22 18
10.8 2.7 0.6 10.6 2.7
7.8 9.5 1.2 8 6.7
8.5 8 3.8 6.5 12.5
7.5 0.5 0.3 13 2
5 2.5 0.6 10.8 2.4
5.1 2.2 1 5.7 1.8
6.5 1.6 0.8
18 19 23 19 8.5
4.7 4.5 2 11.1 7
19 8 24 18 19
CONTROL GLUCOSA MUESTRA

ENVERGADURA TALLO RAIZ ENVERGADURA

8.5 6 7.2 5.5

5.5 9 6.5 5
1.5 6.5 16.8 4
1 5.8 16.5 3
2 4.6 3.3 1
2 4.6 4.5 1.5
3.5 4.3 2
16 4.2 3.3 15
3 4 4.3 0.5
1.5 6.5 7.5 2
11.5 8 10 1.5
8.3 8 8 2
0.8 21 28.5 8
1 6.7 11 2
4 13 10.6 6.1
3.2 18 15 7.8
2.5 8.1 2.5 0.5
1.5 6.6 7.9 0.5
8 11.5 0.5
24 5 8.6 0.4
2.5 5.5 14.5 0.6
23 3 7 3
7 1.5 1
5.2 1.4 1.5
5.5 1.5 0.6
4.8 1 0.5
11 4.5 14
9 5.5 14
 CONTROL

TALLO RAIZ ENVERGADURA CONCENTRACION ABSORBANCIA

8 7.5 3.5 100 0.05

8.5 9 6.5 300 0.122
6.7 16.7 4.5 500 0.301
6 16 3.5
4.4 4.7 1.8
4.8 3.6 2
2.9 2.7 1.4
3.2 3.4 0.3
2.8 2.2 0.4
9 7 1.5
9 10 2
9 10.5 2
25 18.5 10
9.2 8 3
12 10.5 5.2
20.2 16 7.6
7.6 2.5 0.8
6.9 6.8 0.7
4.5 5.2 0.5
5 1.7 1.8
4.5 1.1 1
5.5 10.2 1
6 2 0.5
9.5 4 1
4.5 2.3 0.7
3.8 1.7 1
23 9 12.8
19 7.3 14.8
 Curva de calibración Azucar reductor
0.4
Absorbancias

0.2 f(x) = 0.0006275x - 0.0305833333

0
50 100 150 200 250 300 350 400 450 500 550
Concentración de glucosa (μg/mL)