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ERI-SILK WEAVING: Unique craftsmanship – warmth of people – versatility – economy of the region
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Thursday, December 5, 2019 18 PHMG 04
P R O T E C T I N G FA R M E R S ’ L I V E S A N D T H E E N V I R O N M E N T
C R E AT I N G S O C I A L VA L U E
I N N O VAT I O N → T R A D I T I O N A L P R A C T I C E
This study was conducted to evaluate the chief chemical components in the final product of Eri-Silk from
Assam with reference to their summer production
Aqueous phase
Flash Chromatography
was lyophilized
(Yamazen, Japan)
7.05 g of lyophilized
onion peel extract
(powder form)
(4% W/W)
Flash Chromatography Parameters
Gradient separation of Onion peel extract Column Specification: ODS column (L size),
Time Solvent A Solvent B Flow Rate 50µm
(mins) (Milli Q) % (Methanol) % (ml/min) Bed Size: 35g
Solvents: Mobile phase A (MilliQ), Mobile
0-2 80 20 30 phase B (Methanol)
Flow rate: 30 ml/min.
2-7 80 to 10 20 to 90 30
Wave length : 254nm
Lyophilizer 7-12 10 90 30 AUFS: 0.16
(Operon, Korea) Sample: 100 mg of lyophilized extract was
12-14 10 to 80 90 to 20 30 dissolved in 1 ml of MilliQ and injected into
14-16 80 20 30
column
Flash Chromatography-
Gradient
Peaks
Fraction collection
Five Fractions were selected in regard with the peak obtained (F3, F4, F6, F8 & F9)
Subjected for Mass spectrometry analysis for phytochemical constituents
Mass parameters
Mass spectrometer parameters Gradient Separation
Time Solvent A Solvent B Flow
Ionization Source: Electrospray (min) (%) (%) (µl/min)
ionization (ESI)
0.00 70 30 500
Polarity: Positive ion polarity
1.00 70 30 500
Scan: Enhanced Mass Spectrum (EMS)
12.00 20 80 500
Mass Range: 150-600Da
Rate: 1000Da/sec 15.00 20 80 500
16.00 10 30 500
20.00 10 30 500
Standard Preparation
F4 F6
F8 F9
Quantification of quercetin
Actual Quantity
Sample name RT (min) Peak height (cps) Quantity (ng/mL) µg/mL (18*Fractions)
Quercetin std
10.63 19800000 1000.00 1.00
(1ug/mL)
F3 9.04 1270000 64.14 0.06 1.15
Cynadin-3-glucoside
Cynadin-3(6-Malonyl glucoside)
Delphinidin-3 glucoside
Quercetin
• generating community-based livelihood, strengthening the village economy and supporting clean and green environment
• Onion peels shows the presence of quercetin (the key component for the color), cyanidin 2- glucoside, cyanidin – 3 (6-malonyl
glucoside) and delphinidin 3- glucoside. Critical concentration of these natural dyes are responsible for the creation of finer
shades
• Further analysis has shown the possibility of creating much finer shades
• This study aims at giving information to the concerned authorities for filing geographical indication (GI) for the benefit of BODO
artisans to get the ethnic patent
• Expected to improve the income and add newer markets for better acceptability
Creating a SOCIAL IMPACT ENTERPRISE that is distinctively progressive in its approach towards
community development, combining traditional skills, current scientific advances, design expertise,
and has a pulse of changing fashion trends for both individual and commercial consumers
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Thursday, December 5, 2019 18 PHMG 04
THANK YOU