Bioprocess Engineering

Materi 12
Pengantar Mikrobiologi
  Bioprocess is a specific process that uses complete
living cells or their components (e.g. bacteria,
enzymes, chloroplasts) to obtain desired products
What is a  Bioprocess engineering is the alteration or significance
of renewable thing to create value-added products
Bioprocess  A specialization of Biological Engineering,
Engineering Biotechnology, Chemical Engineering or of Agricultural
Engineering
?  It deals with the shape or style and development of
equipment and processes for the creation of products
such as pharmaceuticals, food, feed, chemicals, and
many polymers and paper from biological materials
  It also deals with studying different biotechnological
processes used in bio industries for large scale
manufacturing of biological product for optimization of
yield and quality in the end product
 Bioprocess Engineers work at the frontiers of biological and
What is a engineering sciences to “Bring Engineering to Life” through
the conversion of biological materials into other forms
Bioprocess needed by mankind
Engineering
In other words:
? Usage of engineering principles or terms to analysis of and
development and design of methods using biological
catalysts such as enzymes, living cells and genetically
engineered cells
  Bread and beer have been
produced since 2400 BC in Egypt

→ The oldest protocol of bread and beer

protocol using yeast

Did you
know?

 In ancient Egypt,
workers recieve beer
as part of their salary
The journey
The journey
 • Therapeutic proteins, antibiotics, antibodies,
Biopharmaceuticals polysaccharides, hormones, vaccines and
diagnostic agents
• Alcohols, organic acids, fuels, several chemicals
and fibers from renewable resource
Industrial chemicals • It is an essential component for rapid transition of
& Energy byproducts from the lab to a manufacturing scale
at a reasonable cost
Current • Alcoholic beverages and dairy products, several
novel foods (soya sauce) and musrooms (starch
Bioprocess Food industries and vitamins) and food additives (anti-oxidants)
and value-added products
Technology • Single cell protein, veterinary vaccine and
& Products Agriculture antibiotics, use of nitrogen fixing bacteria, to
produce a transgenic plants and genetic
modifications of foods

• Wastewater treatment plans to provide clean and

safe drinking water, bio composting methods for
Environmental- waste management
management • Bioprocessing technology on the transformation
of hazardous wastes and the use of biological
process that produce desired products with little
or no waste byproducts
 Major
Products
 From small
scale to large
scale
 Determination of process

Bioprocess optimization in lab scale

(Development of cultivation strategy to reach the maximal productivity)

The Steps
Scaling up of the process

Down stream
(Separation/Isolation/Purification and incerase of products stability)
 Biomass
Enzyme Organisms
feedstock
Example of
food
bioprocessing Pre-treatment Hydrolysis Fermentation Separation Products

Wastewater

Lignin Cells Byproducts

In and Out
Medium should resulted
in :

1) max yield of products

2) max concentration of
products
3) cause min problems
4) cause min yield of
undesirable products
Scaling up
 Plant
Metabolites
Old fashion
way
 New
fashion way
Photobioreactor is
a bioreactor that
utilizes a light source
to cultivate
phototrophic micro
organisms
Bioprocess
in animal
cells
From small
scale to
large scale
Examples
 Insulin
production
in E. coli
Bioreactor
 Examples of bioreactors and product development

Examples
 Some applications of submerged bioreactors

Examples
 Stirred tank bioreactors

Stirred Tank 15 L

Bioreactors
(STR)

150 L bioreactor
1500 L bioreactor
 Bubble column bioreactors

Bubble column
reactor for
algal culture
 Airlift Reactors

Airlift
reactors for
plant and
animal cell
culture
Cost values
 Plant
factory