Académique Documents
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Jose
Postdoctoral Fellow, Department of Biology, IISER Pune, Pune- 411008, India
Email: gregor.jos@gmail.com, gregor@iiserpune.ac.in │ Phone: +91 9836256709
Academic Qualifications:
PhD, Dept. of Biological Sciences, IISER Kolkata, West Bengal, India 2008-2014
M.Sc., Biochemistry, Bharathiar University, Tamil Nadu, India 2004-2006
B.Sc., Biochemistry, University of Calicut, Kerala, India 2001-2004
Research experience:
Post-Doctoral Fellow, Department of Biology, IISER Pune, India 2015-Present
PLiMAP Technology: Peripherally-associated membrane proteins (MAPs) function in a variety of cellular
pathways ranging from cell signaling, host immunity, host-pathogen interaction and vesicular transport.
Identification of MAPs from a complex mix of proteins and functional characterization necessitate
methods that report on binding to membranes of a defined lipid composition. We have developed a
photoactivatable fluorescent phospholipid as a generic reporter of peripheral protein-membrane
interactions. When incorporated into liposomes and exposed to UV, proteins bound to liposomes
become crosslinked with the fluorescent lipid and can be readily detected and quantitated by in-gel
fluorescence analysis. This modification obviates the requirement for high-speed centrifugation
common to most assays that detect liposome-bound proteins. We refer to this assay as Proximity-based
Labeling of Membrane-Associated Proteins (PLiMAP). We are extending this methodology to enrich
membrane associated proteins and identify novel membrane remodeling proteins.
Industrial experience:
Scientist (R& D), Biocon Limited, Bangalore, India 2006-2008
Contributed to the development of Insulin Glargine (BASALOGTM) from laboratory to industrial scale,
which is the first long acting insulin developed and marketed by Biocon Limited.
Publications:
Jose, G. P., Gopan, S., Bhattacharyya, S., Pucadyil, T. J., A facile, sensitive and quantitative
membrane-binding assay for proteins (Manuscript under revision in Traffic)
Nisal, R., Jose, G.P., Shanbhag, C., and Kalia, J., (2018) Rapid and reversible hydrazone
bioconjugation in cells without the use of extraneous catalysts, Org. Biomol. Chem. 16, 4304-
4310.
Bhattacharyya, S., Sarkar, A., Dey, S. K., Jose, G. P., Mukherjee, A., and Sengupta, T. K. (2013)
Copper(II) complex of methionine conjugated bis-pyrazole based ligand promotes dual pathway
for DNA cleavage, Dalton Trans. 42, 11709-11719.
Hatai, J., Pal, S., Jose, G. P., and Bandyopadhyay, S. (2012) Histidine Based Fluorescence Sensor
Detects Hg2+ in Solution, Paper Strips, and in Cells, Inorg. Chem. 51, 10129-10135.
Hatai, J., Pal, S., Jose, G. P., Sengupta, T., and Bandyopadhyay, S. (2012) A single molecule multi
analyte chemosensor differentiates among Zn2+, Pb2+ and Hg2+: modulation of selectivity by
tuning of solvents, RSC Adv. 2, 7033-7036.
Soni, J., Jose, G. P., Ghosh, S., Pradhan, A., Sengupta, T. K., Panigrahi, P. K., and Ghosh, N.
(2011) Probing tissue multifractality using wavelet based multifractal detrended fluctuation
analysis: Applications in precancer detection, In Biomedical Engineering and Informatics
(BMEI), 2011 4th International Conference on, pp 448-452, IEEE
Jose, G. P., Santra, S., Mandal, S. K., and Sengupta, T. K. (2011) Singlet oxygen-mediated DNA
degradation by copper nanoparticles: potential towards cytotoxic effect on cancer cells, J.
Nanobiotechnology 9, 9.
Patents:
Jose, G. P., and Pucadyil, T. J., (2019) A novel bifunctional lipid probe for Proximity Labelling
based Identification of Membrane-Associated Proteins, Application no: 201921022074
(Provisionally patented in India)
Technical Expertise:
Synthesis of UV-activatable and clickable phospholipids
Metabolic labelling of phospholipids with clickable analogs and click reaction-based pull-down of
lipid-binding proteins
Mass spectrometry: QTRAP 4500 MS/MS (SCIEX) for lipidomics and Triple TOF 6600 (SCIEX) for
proteomics
Cell Biology: Cell culture, confocal microscopy, FACS, lentiviral transduction
Molecular Biology: Gene cloning, protein expression and purification, in vitro transcription
Software skills: MaxQuant, LipidView, Image J, Graphpad Prism, Adobe Illustrator
Invited talks:
Presented an invited talk at IISER Kolkata, West Bengal, India entitled “Proximity Labeling-based
Identification of Membrane-associated Proteins” on 11.09.2019.
Professional memberships:
Life member of The Society of Biological Chemists (India)
Life member of Proteomics Society, India
Member of International Chemical Biology Society
REFEREES