Académique Documents
Professionnel Documents
Culture Documents
1
From the Key Laboratory for Clinical Cardiovascular Genetics and
INTRODUCTION Sino-German Laboratory for Molecular Medicine (X-XZ, S-HL, X-XL, and
RH) and the Department of Cardiology (X-XZ, Y-LX, S-HL, RH, and
Cardiovascular disease (CVD) is a leading cause of morbidity,
X-HH), Cardiovascular Institute and FuWai Hospital, Chinese Academy of
mortality, and disability worldwide (1). Hyperlipidemia, which Medical Sciences and Peking Union Medical College, Beijing, China.
results from abnormalities in lipid metabolism, leads to the 2
X-XZ and Y-LX contributed equally to this article.
development of atherosclerotic plaques and is one of the key risk 3
Supported by the Ministry of Science and Technology of China with
factors of CVD (2). Risk of heart attack is 3-fold higher in a grant of the National High-Tech Research and Development Program of
subjects with hyperlipidemia than in subjects with normal lipid China (to X-HH).
4
status (3), whereas a 1% decrease in serum cholesterol has been Address correspondence to X-Ho Huang, Cardiovascular Institute and Fu-
shown to reduce risk of CVD by 3% (1). With the increasing Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Med-
ical College, 167 Beilishilu, Beijing 100037, China. E-mail: huangxhong12@
incidence of hyperlipidemia, more and more consumers are
gmail.com; or R Hui, Cardiovascular Institute and FuWai Hospital, Chinese
aware of the effects of what they eat and drink on their blood lipid Academy of Medical Sciences and Peking Union Medical College, 167
profiles. Beilishilu, Beijing 100037, China. E-mail: huirutai@gmail.com.
Green tea is a widely consumed beverage worldwide and is Received December 21, 2010. Accepted for publication May 16, 2011.
traditionally used in Asian countries as a medication. Green tea is First published online June 29, 2011; doi: 10.3945/ajcn.110.010926.
Am J Clin Nutr 2011;94:601–10. Printed in USA. Ó 2011 American Society for Nutrition 601
602 ZHENG ET AL
Data quality
Results of the validity of included trials are presented in Table
Batista et al, 2009 (11) Double-blinded 33 (5/28) Hypercholesterolemic 8 wk 250 mg GTE capsules Placebo capsules NR Maintain low-fat diet; no vitamin
crossover patients, 21–71 y (catechins NR) supplement
of age
Chan et al, 2006 (12) Double-blinded 34 (0/34) Obese women with 3 mo Green tea capsules Placebo capsules No Limit caffeine; nutritional consults
parallel PCOS, 25–40 y (661 mg catechins)
of age
Diepvens et al, 2006 Double-blinded 46 (0/46) Overweight women, 12 wk GTE capsules (1207 Placebo (maltodextrin) NR Maintain low-energy diet; limit 3
(13) parallel 19–57 y of age mg catechins) capsules cups of coffee/d
Frank et al, 2009 (14) Double-blinded 33 (33/0) Healthy men, 18–55 y 3 wk Aqueous GTE capsule Placebo (maltodextrin) No Maintain usual diet and exercise,
parallel of age (714 mg catechins) capsules limit tea and coffee 3 cups/d
Freese et al, 1999 (15) Double-blinded 20 (0/20) Healthy nonsmoking 4 wk 3 g GTE capsules Placebo (saccharose, No Rich in linoleic acid diet
parallel women, 23–50 y (810 mg catechins) microcrystalline,
of age cocoa) capsules
Fukino et al, 2008 (16) Open-label 60 (51/9) Patients with diabetes 2 mo GTE powder packets No intervention NR Maintain usual diet
crossover or prediabetes, 32–73 y (456 mg catechins)
of age
Hsu et al, 2008 (17) Double-blinded 78 (0/78) Obese women, 16–60 y 12 wk 1200 mg GTE capsules Placebo (cellulose) Yes Maintain normal diet
parallel of age (491 mg catechins) capsules
Maki et al, 2009 (18) Double-blinded 128 (67/61) Overweight or obese 12 wk 500 mL green tea Placebo beverage Yes Maintain normal diet, limit 2
parallel adults, total cholesterol beverage (625 mg caffeinated beverages/d
200 mg/dL, 21–65 y catechins)
of age
Maron et al, 2003 (19) Double-blinded 220 Patients with mild- 12 wk 375 mg GTE capsule Placebo (inert No Maintain low–saturated fat diet
ZHENG ET AL
Jadad score (,4 or 4). Significant reductions in TC and LDL Sensitivity analysis showed that the significance in the pooled
cholesterol were shown in the low- and high-score subgroups. No changes in TC, LDL cholesterol, and HDL cholesterol were not
significant changes in HDL cholesterol were observed in any altered after the imputation correlation coefficient of 0.5. Sensitivity
subgroup. Results are summarized in Table 3. analysis that excluded low-quality studies (11, 13–16, 18–22, 24)
FIGURE 2. Meta-analysis of effects of green tea consumption on total cholesterol (TC) compared with control arms. Sizes of data markers indicate the
weight of each study in the analysis. WMD, weighted mean difference (the result was obtained from a fixed-effects model).
606 ZHENG ET AL
showed that significant results were not influenced concerning TC, showed that these changes were not influenced by the type of
LDL cholesterol, and HDL cholesterol [TC concentration: 23.05 intervention, treatment doses of green tea catechins, study du-
mg/dL (95% CI: 26.62, 0.53 mg/dL), P 0.758; LDL-cholesterol ration, individual health status, or quality of the study.
concentration: 25.25 mg/dL (95% CI: 28.54, 21.95 mg/dL), A large population-based study that involved .40,000 middle-
P = 0.053; HDL-cholesterol concentration: 2.36 mg/dL (95% aged Japanese revealed that, compared with no tea drinking,
CI: 20.36, 5.08 mg/dL); P = 0.833]. The removal of 2 trials (19, habitual green tea consumption [an average of .2 cups (’17
20) with industry funding did not change the final results [TC oz)/d for 10 y] was associated with a lower risk of death from
concentration: 27.16 mg/dL (95% CI: 28.16, 26.17 mg/dL); CVD (5). The beneficial effects of green tea on cardiovascular
P = 0.359; LDL-cholesterol concentration: 22.19 mg/dL (95% health may be due to the high concentration of green tea cat-
CI: 23.16, 21.21 mg/dL); P = 0.201; HDL-cholesterol con- echins, which have been proven to favorably modulate the
centration: 0.06 mg/dL (95% CI: 20.98, 1.11 mg/dL); P = plasma lipid profile. These small molecules exert a variety of
0.263]. The results are also shown in Table 3. Overall, no sig- physiologic actions and, thus, affect lipid metabolism.
nificant heterogeneity was shown for TC, LDL cholesterol, and Animal experiments indicated that the inhibition of cholesterol
HDL cholesterol, and the results were reported on the basis of absorption may be the mechanism to explain the cholesterol-
fixed-effects models. lowering effects of green tea. Catechins with gallate esters were
shown to interfere with the biliary micelle system in the lumen of
the intestine by forming insoluble co-precipitates of cholesterol
Publication bias and increasing the fecal excretion of cholesterol (42). This ap-
Funnel plots and Egger’s tests indicated no significant publi- parent decrease in cholesterol absorption and reduction in liver
cation bias in the meta-analyses of TC, LDL cholesterol, and HDL cholesterol concentrations lead to an increase of LDL-receptor
cholesterol (TC Egger’s test: P = 0.148; LDL cholesterol Egger’s expression and activity (9). This cell-surface protein is present on
test: P = 0.385; HDL cholesterol Egger’s test: P = 0.679). the outer surface of most cells, but in particular liver cells, it can
remove cholesterol-carrying LDL from the circulation. Studies in
animals have provided evidence that green tea extracts and their
DISCUSSION catechin constituents can reduce plasma, liver, and thoracic aorta
Our meta-analysis showed that both green tea beverages and cholesterol and up-regulate hepatic LDL receptors (9, 10). Bursill
green tea extract supplementation significantly reduce blood TC and Roach (9) and Bursill et al (10) have concluded that the
and LDL-cholesterol concentrations but did not affect HDL administration of green tea extract was able to significantly in-
cholesterol concentrations. Subgroup and sensitivity analyses crease both the LDL-receptor binding activity and relative
GREEN TEA LOWERS CHOLESTEROL 607
amounts of LDL-receptor protein. In addition, there is another trations (12, 13, 16, 24). To clarify the precise effect of green tea on
possible major mechanism by which green tea lowers cholesterol: serum cholesterol, we conducted the current meta-analysis of
catechins have direct inhibitory effects on cholesterol synthesis. published RCTs. The results indicated that green tea beverages and
A recent in vitro study has revealed that green tea catechins were green tea extract supplementation significantly reduced TC and
potent and selective inhibitors of squalene epoxidase, which is LDL-cholesterol concentrations. The results of this study were
likely a rate-limiting enzyme of cholesterol biosynthesis (43). consistent with a recently published meta-analysis that showed that
These effects of green tea are similar to hypocholesterolemic green tea significantly reduced LDL-cholesterol concentrations
drugs such as statins, which reduce cholesterol synthesis and (45). Furthermore, a recent study conducted in Japanese children
increase the LDL receptor (44). showed that the consumption of a catechin-rich beverage for 24 wk
The effect of green tea beverages and green tea extract on blood significantly decreased LDL cholesterol after a 12-wk follow-up
lipid profiles has been investigated in vitro and in vivo, including in (46). To our knowledge, this study provided new evidence, which
studies in both animals and humans, by many researchers. Hsu et al supported the conclusion of our meta-analysis, that green tea has
(17) have revealed that green tea intakes significantly decreased hypocholesterolemic properties.
LDL-cholesterol concentrations and markedly increased concen- These results suggested that green tea may be incorporated into
trations of HDL cholesterol. Consistent with this study, other a targeted dietary program as part of public health policy to im-
studies showed that green tea consumption was able to reduce prove cardiovascular health. Because most Americans drink high-
serum cholesterol concentrations (11, 14, 19, 21, 23). In contrast, calorie beverages or alcohol on a daily basis, and only 20% of
several studies reported that there were no positive correlations Americans consume low-calorie green tea (47), the potential for
between green tea intake and reduced blood cholesterol concen- meaningful intervention is real.
608
TABLE 3
Subgroup analyses of total, LDL, and HDL cholesterol stratified by previously defined study characteristics
Total cholesterol LDL cholesterol HDL cholesterol
No. of Mean difference P for No. of Mean difference P for No. of Mean difference P for
Variables trials (95% CI) heterogeneity trials (95% CI) heterogeneity trials (95% CI) heterogeneity
With cardiovascular risks 8 27.58 (28.62, 26.54) 0.519 7 21.85 (22.88, 20.83) 0.246 8 0.40 (20.68, 1.48) 0.106
Duration
,12 wk (low median) 7 23.19 (26.40, 0.02) 0.899 5 24.77 (27.77, 21.77) 0.742 5 0.05 (21.80, 1.90) 0.650
12 wk (high median) 7 27.63 (28.67, 26.58) 0.489 6 21.88 (22.91, 20.85) 0.142 7 0.32 (20.83, 1.48) 0.094
Jadad score
Low (2 or 3) 11 27.55 (28.58, 26.52) 0.617 8 21.89 (22.92, 20.87) 0.789 10 20.07 (21.12, 0.98) 0.300
High (4 or 5) 3 23.05 (26.62, 0.53) 0.758 3 25.25 (28.54, 21.95) 0.053 3 2.36 (20.36, 5.08) 0.833
Sensitivity analysis
High-quality studies 3 23.05 (26.62, 0.53) 0.758 3 25.25 (28.54, 21.95) 0.053 3 2.36 (20.36, 5.08) 0.833
Studies did not receive industry funding 13 27.16 (28.16, 26.17) 0.359 11 22.19 (23.16, 21.21) 0.201 11 0.06 (20.98, 1.11) 0.263