COURSE STRUCTURE

MSc, Microbiology

SEM COMPONENT SUB CODE TITLE OF THE PAPER HRS/ CREDIT

WEEK
Core І 09PMBC11 General microbiology 6 4

Core І І 09PMBC12 Microbial Diversity And Classification 6 4

І Core III 09PMBC13 Bacteriology 6 4

Elective І 09PMBE11 Bioinstrumentation and Techniques 6 5

Practical І General Microbiology, Microbial Diversity 6 -

and Bioinstrumentation

Total 30 17

Core І 09PMBC21 Microbial Physiology and Biochemistry 4 4

Core І I 09PMBC22 Immunology 4 4

Core ІII 09PMBC23 Medical Microbiology 4 4

Core І V 09PMBC24 Microbial Genetics and Molecular Biology 4 4

Elective І 09PMBE21 Computers and Biology 4 4

ІI Practical Exam І 09PMBCR1 Practical- І - 4

Practical Exam 09PMBCR2 Practical- ІI 5 4

ІІ Techniques in Microbial Physiology
Biochemistry and Molecular Biology
Practical Exam
09PMBCR3 Practical- ІII 5 4
ІІІ
Techniques in Immunology and Medical
Microbiology

Total 30 32
 GENERAL MICROBIOLOGY

UNIT : I
Evolution of microbiology- contributions of Van Leeuwenhoek, Joseph Lister, Louis Pasteur
, Robert Koch, Edward Jenner, Winogradsky and Beijernick- Further developments in
microbiology- identification, characterization and classification of microorganisms- Distinguishing
characteristics between prokaryotic and eukaryotic cells- Phenotypic characters- Taxonomic
characters- Distinctive characters of major groups of microorganisms- Principles of classification-
Numerical taxonomy and taxonomic structuring- Genetic relationship- DNA homology- 16S rRNA
Sequencing.

UNIT : II
Microscopy- It’s principles and applications in the field of microbiology including the
following, Dark field, phase contrast, fluorescence microscopy, transmission and scanning electron
microscopy, confocal microscopy- colorimeter, spectrophotometer and lyophilizers- Staining
methods- Gram’s acid- fast, met-achromatic granules, nuclear, capsule, flagella, silver
impregnation and other special staining methods.

UNIT : III
Methods of sterilization: Physical and chemical agents, radiation and filtration- Indicator
microorganisms for sterilization methods- Cultivation of microorganisms- Microbiological media,
enrichment media, enriched media, transport media, selective media and pure culture technique-
Methods of preservation and maintenance of cultures- Role of disinfectants.

UNIT : IV
Bacterial anatomy, structure, properties and bio-synthesis of cellular components of
bacteria- Sporulation and it’s mechanism- Growth and nutrition- Nutritional requirements-
Autotrophs- Heterotrophs- Enrichment cultures- Growth curve- Kinetics of growth- Batch culture-
Synchronous growth- Measurement of growth and enumeration of cells- Techniques of pure
culture.

UNIT : V
Laboratory uses of animals with special reference to microbiology pathogenicity testing,
antibody production, and toxin/ toxoid testing, hypersensitivity testing, and maintenance of
microbes in animals- Care breeding and maintenance of laboratory animals: Rabbits, rats, mice,
guinea pigs, monkeys etc, Disposal of animal house wastes.
 MICROBIAL DIVERSITY AND CLASSIFICATION

UNIT : I

Classification of microorganisms- Introduction- Haeckel’s three kingdom concept-

Whittaker’ five kingdom concept- Three domain concept of Carl Woese basis of microbial
classification, Salient features of bacteria according to Bergey’s manual of determinative
bacteriology- Cyanobacteria- Archaebacteria evolution of archaebacterial membranes, cell wall and
metabolism.

UNIT : II

Fungi: Morphology, taxonomy and classification- detection and recovery fungi from clinical
specimens- Dermatophytes and agents of superficial mycoses- Yeast of medical importance –
Mycotoxins- Anti-fungal agents- Testing methods and quality control. Dimorphic fungi causing
systematic Mycoses- Dermataceous fungi, opportunistic hyaline hypomycetes, agents of
Zygomycoses- Fungi causing eumycotic mycetoma- Immunity to fungal infections.

UNIT : III

Mycology: Historical introduction, structure and cell differentiation- Lichens: Ascolichens,

basidolichens and deuterolichens. Mycorrhiza- Ectomycorrhizae, endomycorrhizae, vesicular
arbuscular mycorrhizae- Fungi as symbionts.

UNIT : IV

Algae: Distribution, general characters, thallus and its structure, classification, nutrition and
reproduction- Characters of selected groups- Blue green algae, Dinofalgellates, Euglenophyta,
Chrysophyta, Phaeophyta and Rhodophyta- Economic importance of alga biotechnology.

UNIT : V

Protozoa Distinguishing characters, classification, host-parasite relationship, pathogenic

mechanism, transmission, life cycle, lab diagnosis, treatment for the following: Entamoeba sp
(Aerobic and anaerobic), Leishmania sp, Trypanasoma sp, Giardia sp, Trichomonas sp and
Balantidium sp. Helminthes: Classification, lifecycle, pathogenesis, transmission, lab diagnosis,
treatment for Cestodes (Taenia solium)- Trematodes ( Fasciola hepatica), Nematodes (Ascaris
lumbricoides, Wuchereia bancrofti)- Arthropod vectors: Tick, mites, fleas, mosquitoes and flies-
Biology, life cycle and transmission diseases to human.
 BACTERIOLOGY

UNIT : I

Classification and identification of bacteria- Bacterial Nomenclature- Bergey’s of

Classification.

UNIT : II

Bacterial Photosynthesis- classification of photosynthetic bacteria- purple bacteria- purple

Sulphur bacteria- purple non sulphur bacteria- Green bacteria- Photosynthetic pigments-
Bacteriochlorophyll metabolism in photosynthetic bacteria.

UNIT : III

Bacteria with unusual properties filamentous bacteria of complex morphology.

UNIT : IV

Bacterial Pathogens- Bacterial toxins- Examples of toxins- caused pathogenesis- bacterial

colonization and invasion.

UNIT : V

Epidemiology of infections diseases- disease reservoirs of infection- modes of transmission-

diseases caused by endospore forming bacteria- enteric bacteria- Mycobacterial disease-
Legionaries disease- listeriosis- brucellosis- Tularemia.
 BIOINSTRUMENTATION AND LABORATORY TECHNIQUES

UNIT : I

pH meter- Titration curve an measurement- Principles, laws of absorption and radiation,

visible, ultraviolet, infrared and mass spectrophotometry- Absorption spectrum, flame photometry-
Principles of colorimetry, turbidometry and viscometry.

UNIT : II

Principles of centrifugation- Centrifugation techniques- Preparative and analytical methods,

density gradient centrifugation- Types of rotors.

UNIT : III

General principle and applications of chromatography- Paper, thin layer, column, gel
filtration, affinity and ion exchange chromatography- Advanced techniques (GC/ HPLC).

UNIT : IV

Electrophoresis- Horizontal and vertical- Moving boundary, zone (paper and gel)
electrophoresis- Immunoelectrophoresis- Rocket electrophoresis- Isoelectric focusing- Application
of each.

UNIT : V

Advanced instrumentation- IR spectroscopy, Raman spectroscopy, x-ray spectroscopy,

NMR, AAS- Principles, component structure and applications. Radio isotopic tracers:
Methodology, problem of experimental design, radiometric analysis, stable and radioactive
isotopes, preparation, labeling, detection and measurement- Autoradiography.