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ACIDS.
STRUCTURE
RNA
DNA
1
Friedrich Miescher in 1869
isolated what he called nuclein from the nuclei of
pus cells
Nuclein was shown to have acidic properties,
hence it became called nucleic acid
2
Two types of nucleic acid are
found
Deoxyribonucleic acid (DNA)
Ribonucleic acid (RNA)
3
The distribution of nucleic
acids in the eukaryotic cell
DNA is found in the nucleus of eukaryotic cells
(with small amounts in mitochondria and
chloroplasts)
RNA is found throughout the cell
4
DNA as genetic material: The
circumstantial evidence
1. Present in all cells and virtually restricted to the nucleus
2. The amount of DNA in somatic cells (body cells) of any given
species is constant (like the number of chromosomes)
3. The DNA content of gametes (sex cells) is half that of somatic
cells.
In cases of polyploidy (multiple sets of chromosomes) the DNA
content increases by a proportional factor
4. The mutagenic effect of UV light peaks at 253.7nm - the peak for
the absorption of UV light by DNA
5
NUCLEIC ACID STRUCTURE
Nucleic acids are polynucleotides
Their building blocks (monomers) are
nucleotides
6
NUCLEOTIDE STRUCTURE
NUCLEOTIDE
7
Ribose is a pentose (5 C sugar)
C5
C4 C1
C3 C2
9
P
THE SUGAR-PHOSPHATE
BACKBONE P
10
P
G
ADDING IN THE
BASES P
C
P
T
11
Hydrogen bonds
P
DNA IS MADE OF G C
P
TWO STRANDS OF P
POLYNUCLEOTIDE C G
P
P
C G
P
P
A T
P
P
T A
P
P
T A
P 12
DNA IS MADE OF TWO STRANDS OF
POLYNUCLEOTIDE
The sister strands of the DNA molecule run in opposite
directions (antiparallel double helix)
They are joined by the hydrogen bonds between bases
Each base is paired with a specific partner:
A is always paired with T
G is always paired with C
(Purine with Pyrimidine)
Thus the sister strands are complementary but not
identical
The bases are joined by hydrogen bonds, individually
weak but collectively strong
13
THE BASES CAN
ONLY PAIR T-A & C-G
14
WEAK HYDROGEN BONDS LINK THE
BASES AND THEREFORE JOIN THE
TWO STRANDS. THE H BONDS CAN ZIP
UNDONE EASILY.
15
Purines Pyrimidines
Adenine Thymine
Guanine Cytosine
16
Wilkins & Franklin (1952): X-ray
crystallography
18
The Double Helix (1953)
19
More about the bases
Hydrogen bonds
20
3′ and 5′ Ribose
22
How is it all
held
together?
23
Histone proteins
support the DNA
The nucleosome is an
8-protein core,
wrapped around a
single protein strand.
The DNA is wrapped
around these
proteins.
24
25
Genes and Bases
26
Exons and Introns
27
Single copy genes
&
Highly repetitive sequences
28
Highly Repetitive Sequences
•The 'gene coding region' (about 1.5 % of our DNA) codes for
a polypeptide (around 25, 000 proteins).
•Around 3% of the human genome is regulatory coding for
genetic switches which control development.
•The non-coding region function remains unclear but can be as
much as 5-45% of the total genome. Not correct to call it
‘Junk DNA’!
•These regions are often made of highly repetitive
sequences of bases each some 5-300 bases long. These are
referred to as satellite regions.
•Due to the combination of bases in the repeating regions they
tend to create dense and less dense DNA regions. These show
as bands of DNA, which are used in finger print technologies.
29