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Laboratory testing guiding principles for patients Specimen collection and shipment
who meet the suspect case definition. Safety procedures during specimen collection
The decision to test should be based on clinical and Ensure that adequate standard operating procedures (SOPs)
epidemiological factors and linked to an assessment of the are in use and that staff are trained for appropriate specimen
likelihood of infection. PCR testing of asymptomatic or collection, storage, packaging, and transport. All specimens
mildly symptomatic contacts can be considered in the collected for laboratory investigations should be regarded as
assessment of individuals who have had contact with a potentially infectious.
COVID-19 case. Screening protocols should be adapted to Ensure that health care workers who collect specimens adhere
the local situation. The case definitions are being regularly rigorously to infection prevention and control guidelines.
reviewed and updated as new information becomes available. Specific WHO interim guidance has been published.16
For the WHO suspected case definition see: Global
Surveillance for human infection with coronavirus disease
Box 1. Biosafety practices in the laboratory
(COVID-2019).8 Testing on clinical specimens from patients meeting the
Rapid collection and testing of appropriate specimens from suspected case definition should be performed in appropriately
patients meeting the suspected case definition for COVID-19 equipped laboratories by staff trained in the relevant technical
and safety procedures. National guidelines on laboratory
is a priority for clinical management and outbreak control and
biosafety should be followed in all circumstances. There is still
should be guided by a laboratory expert. Suspected cases limited information on the risk posed by COVID-19, but all
should be screened for the virus with nucleic acid procedures should be undertaken based on a risk assessment.
amplification tests (NAAT), such as RT-PCR. Specimen handling for molecular testing would require BSL-2
or equivalent facilities. Attempts to culture the virus require
If testing for COVID-19 is not yet available nationally,
BSL-3 facilities at minimum.
specimens should be referred. A list of WHO reference For more information related to COVID-19 risk assessment, see:
laboratories providing confirmatory testing for COVID-19 WHO interim guidance for laboratory biosafety related to 2019-
and shipment instructions are available. nCoV. Samples that are potentially infectious materials (PIM)
for polio need to be handled and stored as described in WHO
document Guidance to minimize risks for facilities collecting,
handling or storing materials potentially infectious for
polioviruses (PIM Guidance) . For general laboratory biosafety
guidelines, see the WHO Laboratory Biosafety Manual, 3rd
edition before the 4th edition is released.
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Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance
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Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance
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Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance
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Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance
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Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance
Wenqing Zhang, Lisa Stevens, Chris Oxenford, Sebastian Céline Barnadas, Maria Van Kerkhove, Lisa Carter,
Cognat, Kazunobu Kojima, Carmen Dolea, Caroline Brown, Mark D Perkins and Karin von Eije.
WHO continues to monitor the situation closely for any changes that may affect this interim guidance. Should any factors change,
WHO will issue a further update. Otherwise, this interim guidance document will expire 2 years after the date of publication
Annex I
COVID-19 VIRUS LABORATORY TEST REQUEST FORM1
Submitter information
Phone number
Specimen information
Type ☐ Nasopharyngeal and oropharyngeal swab ☐ Bronchoalveolar lavage ☐ Endotracheal aspirate ☐
Nasopharyngeal aspirate ☐ Nasal wash ☐ Sputum ☐ Lung tissue ☐ Serum ☐ Whole blood ☐ Urine
☐ Stool ☐ Other: ….
All specimens collected should be regarded as potentially infectious and you must contact the reference laboratory
before sending samples.
All samples must be sent in accordance with category B transport requirements.
Has the patient had contact with a confirmed case? ☐ Yes ☐ No ☐ Unknown ☐ Other exposure:
Additional
Comments
© World Health Organization 2020. Some rights reserved. This work is available under the CC BY-NC-SA 3.0 IGO
licence.
1
Form in accordance with ISO 15189:2012 requirements
2
World Health Organization. Global Surveillance for human infection with coronavirus disease (COVID-19)
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