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Infection Control &

Hospital Epidemiology
( http://www.shea-online.org/)

Volume 14, Issue 9

September 1993 , pp. 527-529

Inactivation of Human Immunodeficiency Virus by a

Medical Waste Disposal Process Using Chlorine
Dioxide
R. Wesley Farr (a1) and Cheryl Walton (a1)
DOI: https://doi.org/10.1086/646798
Published online by Cambridge University Press: 08 April 2017

Abstract
Objective:
To study the ability of a medical waste disposal process using chlorine dioxide to
inactivate human immunodeficiency virus type 1 (HIV 1).
Design:
Stock HIV-1 (HTLV-IIIB strain) was treated with chlorine dioxide under the
following settings: cell culture medium alone, culture medium with 25% blood,
culture medium with medical supplies treated by the Condor machine (Winfield
Environmental Corp., Escondido, CA). MT-2 cells in 96-well tissue culture plates
were inoculated with serial tenfold dilutions of treated and untreated HIV-1.
Cytopathic effect was read on day five, and the TCID50 (50% tissue culture
infectious dose) was calculated.
Results:
Treatment of HIV-1 with chlorine dioxide in culture medium alone resulted in a
5.25 log10 reduction in TCID50. Treatment of HIV-1 with chlorine dioxide in the
presence of 25% blood caused a 6.25 log10 reduction in HIV-1 infectivity Treatment
of HIV-1 with chlorine dioxide in the presence of medical supplies treated in the
Condor machine resulted in a 4.75 log10 reduction in HIV infectivity.
Conclusions:
Chlorine dioxide inactivated HIV-1 in vitro. Chlorine dioxide inactivated HIV-1 in the
presence of blood and in the presence of medical supplies under conditions that
simulated the conditions existing in the Condor machine.
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Copyright
COPYRIGHT: © The Society for Healthcare Epidemiology of America 1993

Corresponding author

Section of Infectious Diseases, Department of Medicine, PO Box 9163, West

Virginia University, Morgantown, WV 26506-9163

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