Loi ndi dau TCVN 8210 : 2008 do Ban KY thuat tiu chudn 10TC-02 Nd cdc va déu dé (B6 Néng nghiép va Phat trién néng than) bién soan, Bd Nang nghiép va Phat trién néng thén dé nghj, Tang cuc Tiéu chudn Bo lveng Chat lueng thdm dinh, B6 Khoa hoc va Céng nghé céng bé.Ngii céc va san pham nghién tir ng coc — Xac dinh d6 axit chuadn 46 Cereals and milled cereal products — Determination of titratable acidity 1 Pham vi ap dung Tiéu chuan nay quy dinh phuwong phap xac dinh d@ axit chudn d ella cde hat ngii céc va cde san phdm nghién ctia ching. 2 Tal ligu vién dan Cac tai liéu vién dan sau rat can thiét cho viée 4p dung tiéu chuan nay. Béi voi cac tai liu vién dan ghi nam céng bé thi 4p dung phién ban duge néu, Déi vai cdc tai liu vién ddan khang ghi nim cang bé thi ap dung phién ban moi nhat, bao gém ca cac stra déi, bé sung (néu ca) TCVN 4851 (ISO 3696), Nudc su dung dé phan tich trong phdng thi nghiam — Yéu cdu Ky thudt va Phwong phap the. TCVN 6451 : 2008 (ISO 13680 : 1999), Nga ode, déu d3 va sdn phdm aghién — Léy mau tie khdi hang tinh, ISO 712 Cereals and cereal products — Datermination of moisture content = Routine reference method (Noi cdc va sdn phdm ngé cdc — Xde dinh dé am — Phuong phap chudn thudng qui}. 3) Thuat ng@ va dinh nghia Trong tigu chuan nay sir dung thuat ngir va dinh nghia sau: 3.4 BG axit chuan dé (Titratable acidity) Thé tich dung dich natri hydraxit (NaQH) can dé trung hoa cac axit cé trong mét Iveng mau tht. CHU THIGH: 6 axit chudn 46 duee biéu thi bang mililil dung dich (NaOH) 0,1 moll trén 100 g mau thé tinh thee chat kha.4 Nguyén tac Chiét tach cdc axit cé trang mau ther bang nue cét trung tinh. Chuan dé phan dich chiét thu duge bang dung dich natri hydroxit 0,1 mall vei chat chi thi phenolphtalein dén khi xuat hién mau hang bén trong 30 s. 5 Thuéc ther Tat cd thude thy dec sir dung phai ld loai khiét phan tich, trir khi cé quy dinh khac. 5.1 Nude, phi hos voi loai 1 cla TCVN 4851 (ISO 3695), d3 loai cacbon dioxit bang cach dun sdéi trong 10 min va dé ngudi truée khi sl dung. §.2 Dung dich natri hydroxit (NaOH) 0,1molil, khang chira cacbonat. Nang 46 chinh xac efia dung dich natri hydroxit dure kiem tra ngay trvde mai day phan tich bang cach si dung dung dich chuan géc axit oxalic hode bang cae dung dich axit chudn khae nhu axit clohydric, axit sulfuric. Tlp dé xac dinh hé sé hiéu chinh néng dé etia dung dich natri hydroxit 0,1 mall. Dung ach da chuan bi duge baa quan trong lo thy tinh mau nau cé nut kin dé tranh su hap thy khi cacbon dioxit trang khang khi. §.3 Chat chi thj phenolphtalein, dung dich 4 % trang etanal Hoa tan 1g phenolphtalein trong 100 ml etanal 95 % thé tich. 6 Thidt bi, dung cu Str dung cac thiét bi, dung cu cla phong thir nghiém théng thudng va cu thé nhw sau: 6.1 Gan phan tich, od thé can chinh xac dén 0,001 g. 6.2. May nghién, cd kha nang nghién mau the lot qua sang cd duéng kinh 16 1,0 mm. 6.3 Sang, co duding kinh 16 1,0 mm. 6.4 May lac, cé tan sé tiv 30 r/min dén 60 cimin, 6.5 Buret, dung tich 25m dure chia 46 0,1 mi va co d6 chinh xac dén 0,05 ml. 6.6 Pipet, dung tich 25 mi va 50 mi. 6.7 Binh dinh murc, dung tich 250 ml. 6.8 Gidy loc, loai trung tinh.6.9 Phéu loc, cd dvdng kinh 7 cm. 6.10 Hép dung mau, co nap day kin. 6.11 Binh nén, dung tich 250 mi. 7 Lay mau Mau goi dén phong thir nghiém phdi la m4u dai dién va khéng bj suy gidm chat lung hay bj thay déi trong qua trinh van chuyén hoac bao quan. Vige lay mau khéng quy dinh trong tiéu chudn nay, Nén lay mau theo TCVN 5451 - 2008 (ISO 13690 : 4999) CHU THICK; Bé axil tang trong qué trinh bao quén, do dé cdc mau thik phai guys gitr trong cac Ip kin, & nhiél dé thich hop va gi ngay dén phang thd nghiém. 8 Chuan bj mau thi» 8.1 84i voi mau dang bét 8.1.1 Mau dang bét cé kich thue lot hoan toan qua sang cé dudng kinh 16 1,0 mm Trén mau da lay theo Biéu 7, chia du va lay knoang 50 g bét va sang qua sang 66 dudng kinh 16 1,0 mm (6.3). Lam vun tat ca nhting phan bot von cue va tron dau. 8.1.2 Mau dang bat tho Trén mau da lay theo Biéu 7, chia ééu va lay it nhdt khodng 100 g dem nghién can than trong may nghién (6.2) che dén khi mu lot hoan toan qua sang co duéng kinh 16 1.0 mm (8.3). 8.2 Bi vei mau dang hat Trgn mau da lay theo Biéu 7, chia déu va ldy it nhat khodng 100 g dem nghién cdn than trong may nghién (6.2) cho dén khi mu I9t hoan toan qua sang cd duéng kinh 18 1,0 mm (6.3). Néu mau cd dé 4m cao, khé nghién can say mau & 80°C trong thdi gian do dé mu tre vé dé 4m thich hop cho viée nghién mau, Trang truéng herp nay, edn str dung hé sé higu chinh vé 66 dm vdi két qua thu dus. 8.3 Mau duoc baa quan trang cac lp dung mau khé sach va cé ndp aay kin, 9 Cach tién hanh 9.4 Trn that kj mu ther dug chuan bi theo Biéu 8, cAn ty 10 g dén 20 g mau, chinh xac dén.0,001 g cha vao Binh dinh mee dung tich 250 ml (6.7). Thém khodng 100 ml nuréc (5.13, day nut, lac tron déutrang 5 min trén may lde (6.4). sau G6 dé yén trang 1h, hoeac trong 7h thinh thaang [dc nhe bang tay Sau dé thém nue oat (5.1) dén vach. Lac déu va loc qua gidy loc (6.8). 9.2. Ding pipet (6,6) ly chinh xac 25 ml hoa 50 ml dich loc (xem §.1) cho vao binh nén dung tich 250 ml (6.11). Thém 5 giot dung dich chi thi phenclphtalein (5.3). Via lac déu binh nan, vira chudn dé lveng axit trong binh nén bang dung dich natri hydroxit 0,1 moll (5.2) én khi xudt hién mau hong bén trong 30 s.. Ghi lai s4 mililit dung dich natri hydroxit 0.1 mol/l da ding dé chudn dé. 9.3 Tién hanh hai pnép xac dinh song song trén cting mét mau the. 10 Tinh kat qua 10.1 6 axit chudn dé tinh thea mau, x), biéu thi bang sé mililit dung dich natri hydroxit 0,1 moll! dé trung héa 100g mau, durc'c tinh theo cong thirc: gio Mea 465 Vyxm trang dd: Vo la thé tieh dung dich natri hydroxit 0,1mell da dting dé chuan d6, tinh bang mililit (mi); Vo la thé tich cla mau thay, tinh bang mililit (ml); Vy lA thé tich mau My dé chudn 44, tinh bang mililit (ml); & la hé sé hiéu chinh nong dé cla dung dich natri hydroxit 0,1 mall: m ta khéi lu@ng mau the, tinh bang gam (g): 100 [4 hé $6 chuyén vé 100 g mau. CHU THICH: Néu mau thé cd a6 dim cao phai say tde khi nghién (8.2) thi cong thies tinh phai quy cdi thee a6 dm cho pho hop wai mau ban dau, Két qua ela phép thé la trung binh céng tia hai ln xac dinh song song trén cling mat mau tht khi sy sai khac clla ching khéng vuot qua 2 % gia tri trung binh. Bao cdo KAt qua chinh xde dén mat chi sd thap phan. 10.2 Bé axit chuan dé tinh theo chAt khd, %}, tinh bang s4 mililit dung dich natri hydroxit 0,1 moll! dé trung héa 100 g mau due tinh thea céng thire:xX «100 et = 100 -w trong dé: Xy 14.60 axit chudn dé dé trung hda 100 g mu & dang phan tich (xem 10.1); w la dg am ela mau thir, tinh bang ghan tram khdi lang (%), xac dinh duc theo ISO 712. 11 Bao cao thiy nghiém Bao cdo thik nghiém phai ghi rd: = moi théng tin can thiet.deé nhan biét dy ai vé mau tha: - phuong phdp lay mau da si dung, néu biét - phuiong phap thy da str dung va vién dan tiéu chudn nay, — két qua thu direc; mei chi tiét thao tac khong quy dinh trang tiéu chudin nay, cing vél cde chi tiét bat thudng khac cé thé anh hung t6i kAt qua.