Stain Purpose Fixation Result Section

Verhoeff Van Gieson stain is most Formalin Elastic fibers black Paraffin
commonly used because it is Nuclei gray to black
quick and produces intense Collagen Red
staining of elastic fibers that are Cytoplasm and muscle
not easily distinguished by H&E yellow
staining.

Verhoeff’s Elastic Method

Orcein is used to stain for elastic Formalin Elastic fibers stain dark Paraffin
fibers, especially in dermatology brown while nuclei are
due to demonstration of the finest stained blue
and most delicate fibers in the
skin

Orcein (Taenzer-Unna Orcein

Method)
Rapid method for staining elastic Elastic fibers appear bright Paraffin
fibers, fibrin, and amyloid red
employing congo red. Fibrin and Connective Tissue
will appear dark blue
RBC will be colored orange-
yellow
Krajian’s Technique
The MSB (Martius, Scarlet Mercuric Chloride Nuclei blue Paraffin
and Blue) method for fibrin is a Erythrocytes yellow
reliable technique. It is more Muscle red
automatic than other Collagen blue
methods, i.e. it is less dependent Fibrin red (early fibrin may
on skill and experience and is stain yellow and very old
consequently suitable for a routine fibrin may stain blue)
laboratory.

MSB Technique