CHAPTER 23

Biochemical Energy Production

Test Bank

TYPE I MULTIPLE-CHOICE QUESTIONS

In each of the following multiple-choice questions, place the letter of the correct response in the blank
at the left. There is only one correct response for each question.

23.1 a [Algorithmic]Which of the following statements about metabolism is correct?

a) Catabolism includes all metabolic reactions in which large molecules are broken
down into smaller ones.
b) Catabolic reaction sequences usually consume energy.
c) Anabolism is the sum total of all chemical reactions that take place within a cell.
d) The process of digestion is an anabolic process.

23.2 d [Algorithmic]Which of the following statements about mitochondria is correct?

a) Mitochondria contain a multi-folded outer membrane and an unfolded inner
membrane.
b) The space between the inner and outer mitochondrial membrane is called the
matrix.
c) The inner mitochondrial membrane separates the matrix and the ATP-synthase
regions.
d) Mitochondria are located within the cellular cytosol.

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260 Chapter 23: Biochemical Energy Production Test Bank

23.3 c Which of the following diagrams gives the correct identities and arrangement of the
structural subunits in coenzyme A?

a) pantothenic acid 2-aminoethanethiol phosphorylated ATP

b) pantothenic acid 2-aminoethanethiol ADP

c) 2-aminoethanethiol pantothenic acid phosphorylated ATP

d) 2-aminoethanethiol pantothenic acid ADP

23.4 c The missing structural subunit in the following diagram for flavin adenine
dinucleotide is

flavin ? phosphate

adenine ribose phosphate

a) phosphate.
b) ribose.
c) ribitol.
d) deoxyribose.

23.5 c The correct notation for the reduced form of nicotinamide adenine dinucleotide is
a) NAD+.
b) NAD.
c) NADH.
d) NADH2.

23.6 c Which of the following is a correct skeletal equation for a hydrolysis reaction
involving adenosine phosphates?
a) ATP  ADP + 2Pi
b) ADP  AMP + 2Pi
c) ATP  AMP + PPi
d) ATP  ADP + PPi

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Test Bank Chapter 23: Biochemical Energy Production 261

23.7 a The “active” portion of coenzyme A is the

a) sulfhydryl group of 2-aminoethanethiol.
b) B vitamin pantothenic acid.
c) phosphate group on carbon 3' of ribose.
d) amino group of 2-aminoethanethiol.

23.8 a The “fuel” for the citric acid cycle is

a) acetyl CoA.
b) citric acid.
c) citrate ion.
d) oxaloacetate ion.

23.9 a Which of the following is a correct general description of the reaction that occurs in
the first step of the citric acid cycle?
a) C2 + C4  C6
b) C3 + C3  C6
c) C2 + C2 + C2  C6
d) C4 + C4  C6 + C2

23.10 c In which two steps of the citric acid cycle are molecules of carbon dioxide produced?
a) steps 2 and 5
b) steps 3 and 5
c) steps 3 and 4
d) steps 4 and 6

23.11 b How many NADH and FADH2 molecules are produced, respectively, during one turn
of the citric acid cycle?
a) 2, 2
b) 3, 1
c) 1, 3
d) 2, 3

23.12 c [Algorithmic]Which of the following citric acid intermediates is not a C4 species?

a) oxaloacetate
b) succinate
c) -ketoglutarate
d) malate

23.13 b [Algorithmic]In which of the following listings of citric acid cycle intermediates are
the compounds listed in the order in which they are encountered in a turn of the
cycle?
a) isocitrate, oxaloacetate, succinate
b) succinate, malate, oxaloacetate
c) fumarate, oxaloacetate, malate
d) malate, fumarate, oxaloacetate

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262 Chapter 23: Biochemical Energy Production Test Bank

23.14 d At which step in the electron transport chain does O 2 participate?

a) first step
b) second step
c) next to last step
d) last step

23.15 c The number of membrane-bound protein complexes associated with the electron
transport chain is
a) one.
b) two.
c) three.
d) four.

23.16 b Which of the following electron carriers is not part of a membrane-bound protein
complex in the electron transport chain?
a) FeSP
b) CoQ
c) cyt c1
d) cyt a3

23.17 b Which of the following sets of electron carriers is associated with the electron
transport chain reactions that occur at protein complex I?
a) CoQ, FeSP, cyt b, cyt c
b) NADH, FMN, FeSP, CoQ
c) cyt c, cyt a, cyt a3
d) FADH2, FeSP, CoQ

23.18 c ATP synthesis via oxidative phosphorylation depends on the passage of which of the
following species through membrane-bound ATP synthase?
a) NADH
b) FADH2
c) H+
d) NAD+

23.19 b [Algorithmic]How many moles of ATP result from the entry of one mole of FADH 2
into the electron transport chain?
a) 1
b) 1.5
c) 2
d) 2.5

23.20 c [Algorithmic]How many ATP molecules result from the “processing” of one acetyl
CoA molecule through the common metabolic pathway?
a) 2
b) 8
c) 10
d) 24

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Test Bank Chapter 23: Biochemical Energy Production 263

TYPE II MULTIPLE-CHOICE QUESTIONS

In each of the following multiple-choice questions, place the letter of the correct response in the blank
at the left. There may be more than one correct response (choice d) or no correct response for a
question (choice e).

23.21 a In a mitochondrion the

a) inner membrane separates the matrix from the intermembrane space.
b) the inner membrane is more permeable than the outer membrane.
c) ATP-synthase complexes are found on the folds of the outer membrane.
d) more than one correct response
e) no correct response

23.22 e Which of the following substances does not contain a B vitamin as a structural
subunit?
a) flavin adenine dinucleotide
b) nicotinamide adenine dinucleotide
c) coenzyme A
d) more than one correct response
e) no correct response

23.23 b Which of the following substances has two unsubstituted ribose molecules as
structural subunits?
a) FADH2
b) NAD+
c) CoA
d) more than one correct response
e) no correct response

23.24 a Which of the following substances contains two or more high energy phosphate
bonds?
a) ATP
b) ADP
c) AMP
d) more than one correct response
e) no correct response

23.25 b Which of the following is a correct letter designation for the reduced form of a
coenzyme?
a) NAD+
b) FADH2
c) NADH2
d) more than one correct response
e) no correct response

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264 Chapter 23: Biochemical Energy Production Test Bank

23.26 e Which of the following are products of the citric acid cycle?
a) acetyl CoA and NAD+
b) acetyl CoA and CO2
c) CO2 and H2O
d) more than one correct response
e) no correct response

23.27 b Which of the following citric acid cycle intermediates is decarboxylated during the
operation of the cycle?
a) oxaloacetate
b) isocitrate
c) succinate
d) more than one correct response
e) no correct response

23.28 d Which of the following citric acid cycle intermediates is a C 4 species?

a) fumarate
b) citrate
c) malate
d) more than one correct response
e) no correct response

23.29 b In which of the following citric acid cycle reactions does the coenzyme FAD
participate?
a) citrate  isocitrate
b) succinate  fumarate
c) malate  oxaloacetate
d) more than one correct response
e) no correct response

23.30 d Which of the following statements concerning citric acid cycle reactions is correct?
a) Two secondary alcohol oxidations occur.
b) Water adds to a carbon-carbon double bond once.
c) Two phosphorylation reactions occur.
d) more than one correct response
e) no correct response

23.31 c Which of the following carry electrons from the citric acid cycle to the electron
carriers of the electron transport chain?
a) CoA
b) NAD+
c) FADH2
d) more than one correct response
e) no correct response

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Test Bank Chapter 23: Biochemical Energy Production 265

23.32 e Which of the following carriers transports electrons from protein complex I to protein
complex III during operation of the electron transport chain?
a) cyt a3
b) cyt c1
c) FMN
d) more than one correct response
e) no correct response

23.33 c Which of the following substances is both a reactant and a product in the operation of
the electron transport chain?
a) O2
b) FADH2
c) CoQ
d) more than one correct response
e) no correct response

23.34 d At which protein complex site in the electron transport chain does proton pumping
occur?
a) protein complex I
b) protein complex II
c) protein complex III
d) more than one correct response
e) no correct response

23.35 d Oxidative phosphorylation is the process in which ATP is synthesized

a) from ATP synthase.
b) from ADP.
c) using energy from the dissipation of a “proton gradient.”
d) more than one correct response
e) no correct response

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266 Chapter 23: Biochemical Energy Production Test Bank

MULTIPLE-CHOICE FORMAT TRUE-FALSE QUESTIONS

In each of the following multiple-choice questions characterize EACH of the three given statements as
being TRUE or FALSE and then indicate the collective true-false status of the statements using the
choices a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.36 c - TFF Statements:

(1) In the last step of the ETC, molecular oxygen is converted to water.
(2) The B vitamin portion of the structure of coenzyme A, which involves
riboflavin, is the reactive site in the molecule.
(3) All of the reactions of the CAC take place in the mitochondrial matrix.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.37 d - FFF Statements:

(1) FADH2 is the oxidized form of the coenzyme flavin adenine dinucleotide.
(2) All of the electron carriers of the ETC contain iron, and one of them also
contains copper.
(3) One turn of the CAC generates two CO2 molecules, two NADH molecules and
two FAD2 molecules.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.38 a - TTT Statements:

(1) Both ribosomes and mitochondria are examples of organelles.
(2) Cytochromes are heme-containing proteins that can undergo reversible
oxidation and reduction.
(3) The electrons that pass through the various steps of the ETC lose some energy
with each transfer along the chain.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

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Test Bank Chapter 23: Biochemical Energy Production 267

23.39 b - TFT Statements:

(1) In the first step of the CAC, a C2 molecule and a C4 molecule combine to
produce a C6 molecule.
(2) ATP, ADP, and AMP differ from each other in the number of adenine
subunits present.
(3) The process by which ATP is produced during the operation of the ETC is
called oxidative phosphorylation.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.40 a - TTT Statements:

(1) High-energy phosphate compounds contain one or more strained bonds which
release above-average amounts of energy when broken.
(2) In the ETC, NADH is oxidized to NAD+.
(3) The “fuel” for the CAC is acetyl CoA.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.41 c - TFF Statements:

(1) The CO2 molecules produced in the citric acid cycle are the CO 2 molecules we
exhale in the process of respiration.
(2) The oxidized form of nicotinamide adenine dinucleotide is NAD + and the
reduced form in NADH2.
(3) During ETC operation, a buildup of protons occurs on the matrix side of the
inner mitochondrial membrane.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.42 c - FTF Statements:

(1) Two ribose subunits are present in the coenzyme flavin adenine dinucleotide.
(2) In the common metabolic pathway, 10 molecules of ATP are produced per
acetyl CoA catabolized.
(3) The purpose of step 2 in the CAC is to convert a secondary alcohol to a
tertiary alcohol.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

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268 Chapter 23: Biochemical Energy Production Test Bank

23.43 a - TTT Statements:

(1) Some, but not all, protein complex sites associated with the ETC serve as
proton pumps.
(2) The rate at which the CAC operates is controlled by the body’s need for ATP.
(3) Anabolic reactions usually require energy input in order to proceed.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.44 b - FTT Statements:

(1) Mitochondria contain a multifolded outer membrane and an inner membrane.
(2) Two of the electron carriers of the ETC transport change are “mobile,”
moving between protein complex sites.
(3) The NADH and FAD2 formed during the CAC carry electrons and H+ ions to
the ETC.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

23.45 b - TFT Statements:

(1) Inhibition of the ETC is the mechanism by which cyanide exerts its deadly
effect.
(2) Brown fat cells contain the uncoupling agent thermogenin, a protein that
creates a proton channel through the outer mitochondrial membrane.
(3) One of the body’s mechanisms for adaptation to cold is the production of
brown fat tissue.
a) All three statements are true.
b) Two of the three statements are true.
c) Only one of the statements is true.
d) None of the statements is true.

MATCHING QUESTIONS

For each of the molecular structural characteristics on the left, select the correct molecule from the
response list on the right. Responses on the right may be used more than once or need not be used at
all.

23.46 b Contains a pantothenic acid subunit ATP

CoA
23.47 b Contains a phosphorylated ribose subunit FAD
NAD+
23.48 a Does not contain a B vitamin as part of its structure

23.49 b Reactive site in the molecule is a –SH group

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Test Bank Chapter 23: Biochemical Energy Production 269

23.50 c Contains a ribitol subunit

For each of the citric acid cycle reactions on the left, select the type of molecules involved (in terms
of number of carbon atoms present) using the response list on the right. Responses on the right may
be used more than once or need not be used at all.

23.51 a Citrate  isocitrate C6  C6

C6  C5
23.52 d Succinate  fumarate C5  C4
C4  C4
23.53 b Isocitrate  -ketoglutarate

23.54 d Malate  oxaloacetate

23.55 d Fumarate  malate

Characterize each of the citric acid cycle reactions on the left as to type of reaction using the response
list on the right. Responses on the right may be used more than once or need not be used at all.

23.56 a Isocitrate  -ketoglutarate a) oxidation of a 2º alcohol

b) oxidation with FAD as the oxidizing agent
23.57 b Succinate  fumarate c) hydration reaction
d) hydrolysis reaction
23.58 d Succinyl CoA  succinate

23.59 a Malate  oxaloacetate

23.60 c Fumarate  malate

For each of the ETC electron carrier characterizations on the left, select the electron carrier’s name
using the response list on the right. Responses on the right may be used more than once or need not
be used at all.

23.61 b Interacts with molecular O2 CoQ

cyt a3
23.62 c Interacts with NADH FMN
FeSP
23.63 d Interacts with FADH2

23.64 b Contains Fe and Cu

23.65 d Contains Fe but not Cu

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270 Chapter 23: Biochemical Energy Production Test Bank

For each of the ETC electron carriers on the left, select the number of ETC protein complexes with
which it is associated using the response list on the right. Responses on the right may be used more
than once or need not be used at all.

23.66 a FADH2 a) one protein complex

b) two protein complexes
23.67 a NADH c) three protein complexes
d) four protein complexes
23.68 c CoQ

23.69 b cyt c

23.70 a cyt b

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