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Cell respiration modeling

I want to make sure you understand the details of cellular respiration

C6H12O6 + 6O2 6H2O + 6O2+energy
Oxidized reduced
1. Write the full, balanced chemical equation for respiration at the top of the page.
a. Draw an arrow to show which reactant becomes which product.
b. Label which reactant is oxidized (loses electrons) and which reactant is reduced
(gains electrons).

2. I have labeled the cytoplasm outside the mitochondrion in this diagram and the mitochondrial
inner and outer membranes. Only some of the folded inner membrane is shown.

Name the two regions inside the mitochondrion (the innermost region and the region between
the two membranes).
mitochondria matrix and the intermembrane space

3. Identify the broad steps of respiration and label where they take place in these different
regions (assume oxygen is available … you are not showing fermentation in this activity)
1-glycolysis-cytoplasm 2-citric acid cycle 3-electron transport chain
For the first two broad steps of respiration, review what chemicals go in to the process, and
what they turn into at the end of the process.
Glycolysis: glucose takes in 2atp and 2phosphates. This is created into 4 ATP’s, two
pyruvates, and 2NADH’s.
Citric Acid Cycle: One carbon from the pyruvates are released to create CO2 and a NADH.
Then it is turned to Acetyl-CoA. Then it attaches to another 4-carbon molecule, and then
released is (2)CO2 and (3)NADH and (1)FADH and 1(ATP). This process is x2. So in goes:
the 2 pyruvates, the 8NAD+, the 2 FAD, the 2ADP and out comes 6CO2, 2 ATP, 8NADH,
and 2FADH.
For the products made in any step, draw arrows to show where that product will go next.
There are two exceptions where you do NOT have to draw arrows:

a. ATP – assume that it just diffuses around the cell, phosphorylating proteins and
chemicals that need energy
b. Water – it just joins the rest of the H2O molecules already present

4. For the electron transport chain and chemiosmosis steps, I have included the proteins
responsible for the process in the mitochondrial inner membrane (with some clues as to
where to start). For these processes, make sure to indicate:

a. where high-energy electrons come from that enter the electron transport chain
(draw dots to represent the electrons as they travel from protein to protein complex in
the chain) … also indicate what happens to the molecule that delivers the high-energy
electrons after it has done its job
b. what those high-energy electrons power as they travel to different protein complexes
c. which direction the protons flow in electron transport chain and chemiosmosis
d. what happens to the low-energy electrons after their energy has been spent
e. name the protein involved in chemiosmosis
f. indicate where a lot of ATP is generated by helping ADP + Pi combine

(note: you only have to do this once … I showed many protein complexes to make
sure you understand that there are LOTS of these proteins embedded in the inner
mitochondrial membrane, not just one set)

5. This modeling activity showed respiration in a eukaryotic cell. Briefly discuss where these
same steps would take place in a prokaryotic bacterium.

Respiration in a eukaryotic cell
mitochondrion outer membrane 2 NADH


\ mitochondrion inner membrane

ADP + Pi enter (x2)
here 1Pyruvate

NADH and CO2

e-s 2Co2 3NADH and
ADP + Pi enter 1FADH
4 carbon used

ADP + Pi H+ H+ pumped to intermembrane.

Electrons move to next protein because each protein
has a higher affinity, they finally get pulled to the O
after energy lost. the energy consumed is used to
pump the H+ up.

NADH & FADH(turn
into FAD and NAD+ O2 2H+ 2e- =electron
ADP + Pi hereand are reused) s
(imagine inner membrane continuing below)