Biochemistry

1. Under Expanded Program on Immunization (EPI) against which of the following

infection/infections a vaccine is administered to neonates:
A. Measles
B. Hepatitis B
C. Poliomyelitis
D. Poliomyelitis and tuberculosis
E. Poliomyelitis and meningitis
2. Communicable diseases are transmitted from one person to another through one or other
route of transmission. Which of the following is the communicable disease?
A. Diabetes mellitus
B. Hypertension
C. Diptheria
D. Cancer
E. Heart disease
3. Which of the following nutritional non essential amino acids is formed from citric acid cycle?
A. Glycine
B. Cysteine
C. Tyrosine
D. Serine
E. Glutamic acid
4. Animation of glutamate to glutamine is catalyzed by:
A. Glutamine synthetase
B. Glutamine dehydrogenase
C. Glutamine oxidase
D. Glutamine transferase
E. Glutamine catalase
5. Urea biosynthesis occurs in:
A. One stage
B. Two stage
C. Three stage
D. Four stage
E. Five stage
6. Enzyme which forms urea is:
A. Ornithine transcarboxylase
B. Arginosuccinate synthetase
C. Arginosuccinase
D. Arginase
E. Urease
7. Reaction of urea cycle takes place in liver cells are:
A. In cytosol
B. Only in lysosomes
C. In mitochondrial matrix
D. Both cytosol and mitochondrial matrix
 E. Both cytosol and lysosomes
8. Which of the following is not used in the synthesis of fatty acids?
A. ATP
B. Cobalamin
C. FADH
D. HCO3
E. NADH
9. Phenylketonuria is an inherited disorder due to deficiency of the enzyme:
A. Transaminase
B. Homogentisic oxidase
C. Phenylalanine hydroxylase
D. Isomerase
E. None of above
10. Long chain fatty acids are synthesized from which of the following:
A. Acetyl CoA
B. Glucose
C. Glycerol
D. Glycogen
E. Succinyl CoA
11. Which of the following enzyme is specific for triacylglycerol synthesis?
A. Acyl CoA synthetase
B. Diacylglycerol acyl transferase
C. Glycerol kinase
D. Glycerol-3-phosphate acyl transferase
E. Monoacyl glycerol acyl transferase
12. Which type of hyperlipoproteinemia is initiated with pancreatic insufficiency?
A. Type I
B. Type II
C. Type III
D. Type IV
E. Type V
13. Amount of ammonia normally present in peripheral blood is:
A. 10-20 mg/dl
B. 5-10 mg/dl
C. 30-40 mg/dl
D. 40-50 mg/dl
E. 1-5 mg/dl
14. The primary substrate for lipogenesis in most mammals is:
A. Acetate
B. Acetyl CoA
C. Citrate
D. Glucose
E. Malonyl CoA
15. Following factors cause hypocholestrolemia:
 A. Mal absorption syndrome and thyroid stimulating hormone
B. Smoking
C. Obstructive jaundice
D. Diabetes mellitus
E. Glucocorticoids
16. Ketone bodies are transported to tissues:
A. Complexed to serum albumin
B. Freely dissolved in blood without attachment to a carrier
C. In chylomicrons
D. In VLDL and LDLs
E. On carnitine
17. The rate limitation step in cholesterol biosynthesis is:
A. Squalne synthetase
B. Mevalonate kinase
C. HMG-CoA synthetase
D. HMG-CoA reductase
E. Thiolase
18. Coding sequence in human RNA are called:
A. Exons
B. Genes
C. Introns
D. mRNA
E. RNA
19. The free fatty acids of plasma are mainly bound to:
A. Albumin
B. Alpha-lipoprotein
C. Beta-lipoprotein
D. Globulin
E. Prothrombin
20. The major source of cholesterol in arterial smooth muscle cell is from:
A. IDL
B. LDL
C. HDL
D. VLDL
E. Chylomicrons
21. Cholesterol is transported in the blood mainly by:
A. Albumin
B. Chylomicrons
C. High density lipoprotein
D. Low density lipoprotein
E. Very low density lipoprotein
22. Regarding De-novo synthesis of purine:
A. Extrahepatic tissues are the main site for formation
B. Formed as adenine and guanine
 C. Carbon dioxide (CO2) fixation reaction requires biotin /ATP
D. Aspartic acid and glycine are completely utilized
E. Inosine monophosphate is converted to AMP and GMP
23. Both DNA and RNA polymerase are able to:
A. Initiate polynucleotide synthesis
B. Remove mononucleotides from 3’ end
C. Remove mononucleotides from 5’ end
D. Synthesize polynucleotides in 5’-3’ direction
E. Catalyze the hydrolysis of pyrophosphate (PP)
24. Which of the following is not characteristic of bacterial ribosome?
A. Sensitivity to streptomycin
B. ITwo subunits
C. SS RNA
D. 16 S RNA
E. 18 S RNA
25. IV pyelography is contraindicated if a person is suffering from:
A. Renal calculi
B. Urinary infection
C. Hematuria
D. Prostatic enlargement
E. Acute nephritis
26. Polymerase chain reaction involves three basic steps in which of the following orders:
A. Anealing denaturation extension
B. Anealing extension denaturation
C. Denaturation anealing extension
D. Extension anealing denaturation
E. Extension denaturation anealing
27. The enzyme involved in differential diagnosis of jaundice is:
A. AST
B. ALT
C. ALP
D. SDH
E. OCT
28. Termination codons recognized by release factor during translation are UGA, UAG and:
A. AUG
B. GAG
C. GGC
D. GUC
E. UAA
29. Which enzyme assay is performed for investigation of extra-hepatic obstruction?
A. Aspartate transaminase
B. Alanine tranaminase
C. Ornithine carbonyl transferase
D. Alkaline phosphatase
 E. Sorbitol dehydrogenase
30. Which test measures the renal plasma flow?
A. Urea clearance test
B. Creatinine clearance test
C. Para amino hippurate test
D. 15 min PSP excretion test
E. None of above
31. Enzyme which joins DNA molecule it is called:
A. DNA ligase
B. DNA lyase
C. DNA polymerase
D. Restriction endonuclease
E. Reverse transcriptase
32. Filtration fraction decreases in:
A. Glomerulonephritis
B. Congestive cardiac failure
C. Essential hypertension
D. Malignant hypertension
E. Severe hemorrhage
33. Transcription:
A. A process catalyzed by DNA polymerase
B. A process that takes place in mitochondria
C. A process of formation of mRNA from DNA
D. A process of formation of all types of RNA from DNA
E. Three different RNA polymerase take part in prokaryotes
34. Sickle cell anemia is the clinical manifestation of homozygous genes for an abnormal Hb
molecules, the mutational event responsible for nutrition in the beta chain is:
A. Deletion
B. Insertion
C. Point mutation
D. Crossing over
E. Non-dysfunction
35. Transcription is the process of synthesis of:
A. mRNA
B. tRNA
C. hnRNA
D. rRNA
E. RNA
36. Post transcriptional modification takes place in:
A. Nucleas
B. Cytoplasm
C. Endoplasmic reticulum
D. Golgi apparatus
E. Lysosomes
37. How many proteins are involved in the replication of E-coli chromosome?
A. 30 proteins
B. 50 proteins
C. 70 proteins
D. 20 proteins
E. 100 proteins
38. Genetic code for methionine is:
A. ACU
B. ACC
C. ACA
D. ACG
E. AUG
39. Which of the following DNA dependent DNA polymerase is for mitochondrial synthesis:
A. Alpha
B. Beta
C. Gamma
D. Delta
E. Epsilon
40. The nearly formed protein in translation containing:
A. Primary structure
B. Secondary structure
C. Tertiary structure
D. Quaternary structure
E. Pentanary structure
41. Translocase is required in the process of:
A. Replication
B. Transcription
C. Initiation of protein synthesis
D. Elongation of peptides
E. Termination of protein synthesis
42. Which of the following condition leads to fat accumulation in the liver, hyperlipidemia and
ultimately cirrhosis?
A. Hepatitis
B. Alcoholism
C. Oxidation of ethanol
D. Acidemia
E. Uremia
43. Which of the following diagnostic enzyme generate phosphate ions from organic phosphate in
osteoblastic mineralization:
A. SGPT
B. SGP1T
C. Alkaline phosphatase
D. Acid phosphatase
E. Creatinine phosphokinase
44. In glomerular filtration, which of the major plasma proteins passes through the normal
glomerulus:
A. Albumin
B. Globulin
C. Local protein
D. Casein
E. Glycoprotein
45. On complete oxidation one mole of palmitic acid gives:
A. 5 ATPs
B. 28 ATPs
C. 106 ATPs
D. 108 ATPs
E. 131 ATPs
46. Maple syrup urine disease is the disease of:
A. Methionine
B. Tryptophan
C. Tyrosine
D. Phenylalanine
E. Branched chain amino acids
47. Which of the following is the fate of NH3:
A. Formation of urea
B. Formation of glutamine
C. Formation of non-essential amino acids
D. All of above
E. None of above
48. Neimann-Pick disease is due to deficiency of the enzyme:
A. Ceramidase
B. Ceramid lactosidase
C. Hexosaminidase A
D. Hexosaminidase B
E. Sphingomyelinase
49. Which one of the following compound is synthesized from Tryptophan?
A. Catecholamines
B. Melanin
C. Melatonin
D. Phenol and cresol
E. Tyramine
50. Which of the following amino acids become essential in phenylketonuria (PKU)?
A. Threonine
B. Tyrosine
C. Phenylalanine
D. Methionine
E. Cysteine
51. Lovastatin lowers the cholesterol level by:
 A. Activating HMG CoA reductase
B. Activating HMG CoA synthetase
C. Inhibiting Acetyl CoA carboxylase
D. Inhibiting HMG CoA reductase
E. Inhibiting HMG CoA synthetase
52. Urea cycle:
A. Is energy supplying mechanism
B. Converts urea into uric acid
C. Converts ammonia into urea
D. Provides arginine in infants
E. Takes place in liver and brain
53. Which of the following organ has a low activity of glycerol kinase?
A. Adipose tissue
B. Brain
C. Intestine
D. Kidney
E. Liver
54. In De-novo synthesis of fatty acids, end product is:
A. Acyl CoA
B. Acyl enzyme
C. Free palmitate
D. Palmitoyl CoA
E. Triacylglycerol
55. The major source of cholesterol in arterial smooth muscle cell is from:
A. IDL
B. LDL
C. HDL
D. VLDL
E. Chylomicrons
56. Neimann-Pick disease is due to deficiency of the enzyme:
A. Ceramidase
B. Ceramid lactosidase
C. Hexosaminidase A
D. Hexosaminidase B
E. Sphingomyelinase
57. Beta oxidation of odd carbon fatty acids chain produce:
A. Succinyl-CoA
B. Propionyl-CoA
C. Acetyl-CoA
D. Malonyl-CoA
E. Acetoacetyl-CoA
58. The free fatty acids in blood are:
A. ………. Is fatty
B. Mainly bound to beta-lipoprotein
 C. Mainly bound to serum albumin
D. Metabolically more active
E. All of the above
59. The immediate substrate for lipogenesis in most mammals is:
A. Acetate
B. Acetyl CoA
C. Cerrate
D. Glucose
E. Malonyl CoA
60. On complete oxidation, one mole of palmitic acid gives:
A. 5 ATPs
B. 28 ATPs
C. 106 ATPs
D. 108 ATPs
E. 131 ATPs
61. APO C-II acts as:
A. Cholesterol ester transfer protein
B. Co factor for LCAT
C. Co factor for lipoprotein lipase
D. Inhibitor for LCAT
E. Inhibitor for lipoprotein lipase
62. The end product of oxidation of long chain fatty acids is:
A. Acetyl CoA
B. Malonyl CoA
C. Methyl malonyl CoA
D. Propionyl CoA
E. Succinyl CoA
63. Hepatic lipase:
A. Is concerned with chylomicron remnant and HDL metabolism
B. Is concerned with chylomicron remnant and VLDL metabolism
C. Is concerned with IDL and HDL metabolism
D. Reacts readily with chylomicron
E. Reacts readily with VLDL
64. Which of the following compound is produced in oxidation of odd chain fatty acids along with
Acetyl CoA as end product:
A. Acetoacetyl CoA
B. Malonyl CoA
C. Methyl malonyl CoA
D. Propionyl CoA
E. Succinyl CoA
65. Fatty acid synthase complex:
A. Is dimer of unsimilar subunits
B. Is composed of six enzymes and an acyl carrier protein
C. Acyl carrier protein contains a phosphate group
 D. Is active when it is dephosphorylated
E. All the components are covalently linked in a single polypeptide chain in dimer form
66. Alkaptonuria is due to the absence of enzyme:
A. Alkaline phosphatase
B. DOPA decarboxylase
C. Homogentisic acid oxidase
D. Phenylalanine hydroxylase
E. Tyrosinase
67. The drug allopurinol is beneficial for the patient with gout because it inhibits the enzyme:
A. Hypoxanthine-guanine phospho reductase transferase (HGPRT)
B. Xanthine reductase
C. Phosphoribosyl pyrophosphate……. Synthetase
D. Purine nucleoside phosphoribosylase
E. Xanthine oxidase
68. The anticodon is an important part of the structure of:
A. r-RNA
B. t-RNA
C. m-RNA
D. hn-RNA
E. DNA
69. Partial defect in enzyme of urea cycle leads to:
A. Decrease in aspartate concentration
B. Excess accumulation of urea
C. High concentration of uric acid
D. High blood level of ammonia
E. Increase in the amount of alpha-ketoglutarate
70. RNA primers are short strands needed for DNA replication. These primers are synthesized by:
A. DNA polymerase
B. Helicase
C. Primase
D. RNA polymerase
E. Topoisomerase
71. Formation of melanin from tyrosine requires:
A. Diamino oxidase
B. Dopa decarboxylase
C. Melanase
D. Peroxidase
E. Tyrosinase
72. Gene determines the behavior of cell by directly control the synthesis of:
A. Cholesterol
B. Carbohydrates
C. Lipids
D. Nucleic acids
E. Proteins
73. Regarding oxidation of fatty acids:
A. Alpha oxidation produces di carboxylic acid
B. Beta oxidation is a process that exclusively oxidizes fatty acids containing even number
of carbon atoms
C. Carnitine palmitoyl transferase 1 (CPT-I) is inhibited by malonyl CoA
D. Oxidation of unsaturated fatty acids occurs in peroxisomes
E. Wellweger's syndrome is caused by deficiency of alpha oxidase enzyme
74. The plasma FFA level may rise to as high as 2.0 mu/Eq /min in:
A. Fasting condition
B. Fully fed state
C. Normal condition
D. Uncontrolled diabetes mellitus
E. Well controlled diabetes mellitus
75. In transmethylation, methyl group is transferred from:
A. Cystine
B. Methionine
C. S-adenocyl ccystine
D. S-adenocyl homocysteine
E. S-adenocyl methionine
76. Triacylglycerol present in plasma lipoproteins can be hydrolyzed by:
A. Colipase
B. Heparin
C. Hormone sensitive lipase
D. Lipoprotein lipase
E. Pancreatic lipid
77. The carbon and nitrogen atoms for the synthesis of pyrimidine ring are derived from:
A. Aspartate and carbonmyl phosphate
B. Aspartate and glycine
C. Glutamate and carbamoyl phosphate
D. Glutamine and glycine
E. Glycine and carbamoyl phosphate
78. Synthesis of 1 mole of urea requires:
A. 1 mole of ATP
B. 2 moles of ATP
C. 3 moles of ATP
D. 4 moles of ATP
E. 5 moles of ATP